Short-chain Organic Acids Research Articles

Deep serum lipidomics identifies evaluative and predictive biomarkers for individualized glycemic responses following low-energy diet-induced weight loss: a PREVention of diabetes through lifestyle Intervention and population studies in Europe and around the World (PREVIEW) substudy

BackgroundWeight loss through lifestyle interventions, notably low-energy diets, offers glycemic benefits in populations with overweight-associated prediabetes. However, >50% of these individuals fail to achieve normoglycemia after weight loss. Circulating lipids hold potential for evaluating dietary impacts and predicting diabetes risk. ObjectivesThis study sought to identify serum lipids that could serve as evaluative or predictive biomarkers for individual glycemic changes following diet-induced weight loss. MethodsWe studied 104 participants with overweight-associated prediabetes, who lost ≥8% weight via a low-energy diet over 8 wk. High-coverage lipidomics was conducted in serum samples before and after the dietary intervention. The lipidomic recalibration was assessed using differential lipid abundance comparisons and partial least squares discriminant analyses. Associations between lipid changes and clinical characteristics were determined by Spearman correlation and Bootstrap Forest of ensemble machine learning model. Baseline lipids, predictive of glycemic parameters changes postweight loss, were assessed using Bootstrap Forest analyses. ResultsWe quantified 439 serum lipid species and 9 related organic acids. Dietary intervention significantly reduced diacylglycerols, ceramides, lysophospholipids, and ether-linked phosphatidylethanolamine. In contrast, acylcarnitines, short-chain fatty acids, organic acids, and ether-linked phosphatidylcholine increased significantly. Changes in certain lipid species (e.g., saturated and monounsaturated fatty acid-containing glycerolipids, sphingadienine-based very long-chain sphingolipids, and organic acids) were closely associated with clinical glycemic parameters. Six baseline bioactive sphingolipids primarily predicted changes in fasting plasma glucose. In addition, a number of baseline lipid species, mainly diacylglycerols and triglycerides, were predictive of clinical changes in hemoglobin A1c, insulin and homeostasis model assessment of insulin resistance. ConclusionsNewly discovered serum lipidomic alterations and the associated changes in lipid-clinical variables suggest broad metabolic reprogramming related to diet-mediated glycemic control. Novel lipid predictors of glycemic outcomes could facilitate early stratification of individuals with prediabetes who are metabolically less responsive to weight loss, enabling more tailored intervention strategies beyond 1-size-fits-all lifestyle modification advice.The PREVIEW lifestyle intervention study was registered at clinicaltrials.gov as NCT01777893 (https://clinicaltrials.gov/study/NCT01777893).

Fermentability assessment of selected oligosaccharides by Lactiplantibacillus plantarum BIOTECH 1074 towards short-chain organic acid production with anti-vibrio efficacy

Vibriosis, caused by Vibrio harveyi and Vibrio parahaemolyticus, poses a significant threat to aquaculture, resulting in substantial economic losses. Oligosaccharides are known for their ability to improve gut health and prevent vibriosis in aquaculture species. This study explored the fermentative utilization of galacto-oligosaccharides (GOS), manno-oligosaccharides (MOS), and inulin by Lactiplantibacillus plantarum BIOTECH 1074. GOS and glucose were the preferred substrates for L. plantarum growth, producing high levels of lactic acid, propanoic acid, and acetic acid. These compounds showed substantial anti-Vibrio activities, having inhibitory concentration values of 0.252 ± 0.005 and 0.656 ± 0.015 mg total organic acid/mL against V. harveyi, V. cholerae, and V. parahaemolyticus from GOS fermented supernatant. The fermentative capabilities of L. plantarum BIOTECH 1074, in utilizing specific oligosaccharide substrates to produce organic acids with anti-Vibrio potential, offers a promising strategy for enhancing disease management and overall efficiency in aquaculture systems.

CeO2-CuO composites prepared via supercritical antisolvent precipitation for photocatalytic hydrogen production from lactic acid aqueous solution

The global increase in energy demand requires a continuous search for renewable and clean alternative resources to fossil fuels. Hydrogen is emerging as a promising energy carrier for the future; its production via photocatalysis, driven by sunlight, can directly convert solar energy into a usable or storable energy resource. However, water splitting requires sacrificial agents or electron donors/hole scavengers, such as short-chain organic acids. This research explores the use of lactic acid as a source for photocatalytic hydrogen production, offering valuable alternatives for wastewater management and renewable energy production. This study employed the innovative supercritical antisolvent (SAS) technique to micronize the precursors of both the active phase (CeO2) and co-catalyst (CuO), ensuring rapid and complete solvent removal and size reduction of photocatalyst precursors. The prepared samples were characterized by field emission scanning electron microscopy (FESEM), Fourier transform infrared (FT-IR) spectroscopy, dynamic light scattering (DLS) analysis, Brunauer-Emmett-Teller (BET) analysis and thermogravimetric analysis (TGA). This study has shown that the micronization process resulted in a notable improvement in CeO2 photocatalytic activity, attributed to the reduction of the dimensions of the powders. Hydrogen production was equal to 3989 μmol L−1 for the SAS-produced photocatalyst while using a commercial CeO2 sample resulted in H2 production of 2519 μmol L−1. The enhanced photoactivity of CeO2-CuO composites was found to be related to the presence of CuO. The optimal CuO amount was equal to 0.5 wt%, determining a hydrogen production of 9313 μmol L−1 after 4 h of UV irradiation time. A photocatalytic test carried out with deuterated water (D2O) instead of distilled H2O demonstrated that hydrogen was preferentially produced from water splitting reaction, whereas lactic acid acted as a sacrificial agent being oxidized from positive holes photogenerated in the valence band of CuO.

Effect of a probiotic mixture with lactic acid activity on productive and allometric indicators in broiler chickens.

The feeding and sanitary conditions significantly influence the productivity of farm animals. This study aimed to assess the impact of a lactic acid-producing microbial additive on broiler chicken productivity. A 42-day experimental period utilized 120 1-day-old Cobb 500 chicks with an average weight of 46 g. In groups of 30 each, the chicks were randomly assigned to four experimental designs. The following treatments were assessed: T1 without intervention (control), T2 with bacitracin at a concentration of 0.5 g/L, T3 with a 5% probiotic mixture (PM), and T4 with a 7.5% PM. The birds were fed the commercial balanced feed without anticoccidials daily, while vaccines were administered according to the recommended biosecurity plan by the commercial house. Drinking water was treated with PM containing lactobacilli, yeasts, and short-chain organic acids. In T4, a 7.5% PM resulted in a final weight of 2361.2 g (p < 0.05), a total weight gain of 1412.8 g (p < 0.05), and improved feed efficiency with a feed conversion of 2.00 (p < 0.05), during which feed intake was lower than in the other groups. Microbial additives with lactic acid activity are a cost-effective and feasible solution for broiler chicken productivity.

Pre‐treatment with Trichoderma viride: Towards a better understanding of its consequences for anaerobic digestion

AbstractUnderstanding and optimising biological pre‐treatment strategies for enhanced bio‐methane production is a central aspect in second‐generation biofuel research. In this regard, the application of fungi for pre‐treatment seems highly promising; however, understanding the mode of action is crucial. Here, we show how aerobic pre‐treatment of crystalline cellulose with the cellulolytic Trichoderma viride affects substrate degradability during mesophilic, anaerobic digestion. It could be demonstrated that fungal pre‐treatment resulted in a slightly reduced substrate mass. Nevertheless, no significant impact on the overall methane yield was found during batch fermentation. Short chain organic acids accumulation, thus, overall degradation dynamics including methane production kinetics were affected by the pre‐treatment as shown by Gompertz modelling. Finally, 16S rRNA amplicon sequencing followed by ANCOM‐BC resulted in up to 53 operative taxonomic units including fermentative, syntrophic and methanogenic taxa, whereby their relative abundances were significantly affected by fungal pre‐treatment depending on the duration of the pre‐treatment. The results demonstrated the impact of soft rot fungal pre‐treatment of cellulose on subsequent anaerobic cellulose hydrolysis as well as on methanogenic activity. To the best of our knowledge, this is the first study to investigate the direct causal effects of pre‐treatment with T. viride on basic but crucial anaerobic digestion parameters in a highly standardised approach.

The main factors that improve gut microbiota composition

The aim is to observe and compare the main factors that can improve gut microbiota composition. Materials and methods. The literature study research was performed in PubMed and Google Scholar electronic databases. We assessed more than 300 studies, data from 65 of which were included in this review. They are presented in three tables: nonpharmacological influence on gut microbiota composition, drugs impact on gut microbiota, and medicines prescribed for gut microbiota correction. Results. On the one hand, non-pharmacological methods of gut microbiota improvement are the safest and the most traditional: healthy diet and physical activity, good sleep, avoiding stress and bad habits, but they are the most difficult for patients’ fulfillment and doctors’ observation. All listed are the components of a healthy way of life and should be followed by everybody. The most prescribed drugs have a significant influence on gut microbiota composition, so physicians should consider their effects in prescriptions. They are antibiotics, steroids and non-steroids, proton pump inhibitors, laxatives, antidepressants, etc. On the other hand, despite the diversity of available medicines (prebiotics, probiotics, paraprobiotics, postbiotics, synbiotics, and antibiotics) that can be used for gut microbiota improvement, all of them are under investigation and need further evaluation. The trendiest medicines for today are paraprobiotics and postbiotics. Paraprobiotics are represented by heat / ultraviolet / sonication Lactobacillus spp., Bifidobacterium spp., and Saccharomyces strains. Postbiotics are performed by short-chain fatty acids, secreted biosurfactants, secreted proteins, organic acids, amino acids, bacteriocins, vitamins, and peptides. Most of the data on their pharmacodynamics is based on animal studies or experimental research, so they need further investigations. Fecal gut microbiota transplantation is also an up-to-date method for multiple disease correction but is approved only for the treatment of recurrent and refractory infections caused by Clostridium difficile. Conclusions. Gut microbiota composition improvement methods are an up-to-date topic for practical medicine because gut microbiota changes are closely linked with host health status. Gut microbiota violations lead to metabolic, cardiovascular, neurological, inflammatory disorders, etc. Nowadays the healthy way of life is the best gut microbiota composition improvement method, but prebiotics, probiotics, paraprobiotics, postbiotics, synbiotics, antibiotics supplementation, and fecal microbiota transplantation also take place and have their indisputable advantages in special cases. Unfortunately, most pharmacological methods of gut microbiota modulation have a weak evidence base. Therefore, this question needs further research in appropriate patient groups with long-term monitoring.

Combining pre-fermentation and microbial electrolysis for efficient hydrogen production from food wastewater

Energy-rich food wastewater (FWW) offers a sustainable feedstock for bioelectrochemical hydrogen production. However, its complex characteristics and high suspended solids content hinder its direct utilization in dual-chamber microbial electrolysis cells (MECs) for producing high-purity hydrogen. This study explored pre-fermentation under uncontrolled pH conditions as a method to improve the exoelectrogenic utilization of FWW in dual-chamber MECs. Across 2–5 d hydraulic retention times (HRTs), pre-fermentation was effective in conditioning FWW to a more favorable composition for exoelectrogenic utilization. Short-chain organic acids and ethanol constituted 48–72% of the total chemical oxygen demand (COD) in pre-fermentation effluents, a substantial increase from 27% in raw FWW. In subsequent MEC experiments, the MECs fed with pre-fermentation effluents exhibited significantly higher current-generating and hydrogen-producing performance compared to those fed with raw FWW. The MECs fed with the 2-d HRT pre-fermentation effluent showed the highest hydrogen yield of 911 mL/g COD fed, which represents a 34.4% increase compared to the MECs fed with raw FWW, resulting in a 1.28-fold higher recovery of chemical energy from organic matter in raw FWW as hydrogen. The anode bacterial community was dominated by exoelectrogenic Geobacter in all MECs, with its abundance being significantly higher when utilizing pre-fermentation effluents (69.0–78.0%) compared to raw FWW (20.6–39.2%). Overall, pre-fermentation proved an effective conditioning approach for enhancing the exoelectrogenic utilization of FWW in MECs.

Exploring functional metabolites and proteomics biomarkers in late-preterm and natural-born pigs.

Pigs are often used to study the intestinal development of newborns, particularly as preterm pig models that mimic the intestinal growth of human preterm infants. Neonatology's study of delivery mode's impact on neonatal development is crucial. We established 14 newborn pigs delivered via cesarean sections (C-section, at 113 days of gestational age, CS group) and 8 naturally born pigs were used as controls (at 114 days of gestational age, NF group). The impact of two alternative delivery procedures (C-section and natural birth) on the levels of short-chain fatty acids (SCFAs) and organic acids in the hepatic and intestines of newborn pigs were compared using metabolomics. The underlying molecular pathways are examined at the "protein-metabolite" level by integrating proteomic data. The findings demonstrated that the mode of delivery changed the metabolism of SCFAs in newborn pigs, perhaps by affecting the physiology levels of cyclic intermediates such as lactate and malate in the pyruvate metabolic pathway. Additionally, by participating in the fatty acid metabolism pathway, two distinct proteins (FASN and HSD17B4) may impact the physiological concentration of these tiny metabolites. In conclusion, this study provided reliable animal model data for understanding the physiological SCFA metabolic information and its affecting mechanism of large-gestational age preterm infants.

UV-A assisted catalytic wet peroxide oxidation: Activity of iron minerals in the degradation of DEET

The widespread use of insect repellent N,N’-diethyltoluamide (DEET), along with its low biodegradability, has made this an ubiquitous contaminant in waterbodies. This work explores the elimination of DEET (10 mg/L) in aqueous matrices employing natural iron minerals ilmenite (FeTiO3), hematite (Fe2O3), goethite (Fe(OOH)) and magnetite (Fe3O4) as low-cost catalysts in UV-assisted Catalytic Wet Peroxide Oxidation, using UV-A LEDs (λ: 395 nm) as radiation source in a 500 mL useful-volume reactor. Under the studied conditions, magnetite presented a higher activity, significantly superior to that of the other minerals. A parametric study evaluating the effect of the catalyst load, initial pH, H2O2 dose, UV irradiance and water matrix was performed. Magnetite is able to carry out DEET degradation working at circumneutral pH (pH: 5) with outstanding results in terms of DEET removal even in complex matrices such as river water and WWTP effluent. Furthermore, the catalyst presented an excellent stability. Still, under the optimal operating conditions of this study ([Fe3O4]: 500 mg/L, [H2O2]0: 61.1 mg/L, pH0: 5, T: 25ºC, t: 120 min), only 27.8% mineralization was reached in ultrapure water. Nonetheless, the remaining organic compounds are mainly short-chain organic acids (malonic, oxalic, acetic and formic acids), which account for 98.7% of the residual organic carbon. These compounds, which are readily biodegradable, present a lower phytotoxicity than that of DEET, demonstrating the potential of UV-CWPO for the elimination of this emerging contaminant.

Preparation of novel Fe-containing zeolite-A for KN-R decolorization by Fenton-like reaction.

Herein, novel catalysts of Fe-containing zeolite-A (Fe/zeolite-A) were synthesized by exchanging iron ions into zeolite-A framework, and short-chain organic acids (SCOAs) were employed as chelating agents. Reactive Brilliant Blue KN-R (KN-R) was used as a model pollutant to evaluate the performance of these catalysts based on the heterogeneous Fenton reaction. The results showed that Fe-OA/3A, which applied zeolite-3A as the supporter and oxalic as the chelating agent, presented the most prominent KN-R decolorization efficiency. Under the initial pH of 2.5, 0.4 mM KN-R could be totally decolorized within 20 min. However, the mineralization efficiency of KN-R was only 58.2%. Therefore, anthraquinone dyes were introduced to modify zeolite-3A. As a result, the mineralization efficiency of KN-R was elevated to 92.7% when using Alizarin Violet (AV) as the modifier. Moreover, the modified catalysts exhibited excellent stability, the KN-R decolorization efficiency could be maintained above 95.0% within 20 min after operating for nine cycles. The mechanism revealed that the Fe(II)/Fe(III) cycle was accelerated by AV-modified catalyst thus prompting the KN-R decolorization in Fenton-like system. These findings provide new insights for preparing catalysts with excellent activity and stability for dye wastewater treatment.

Application of catalytic wet peroxide oxidation for sunscreen agents breakdown

Sunscreen agents are chemical compounds widely used nowadays for skin protection from UV sunlight. Recently, their ubiquitous occurrence in aquatic systems has been evidenced, which poses a high risk for the environment and human health as they are associated with endocrine disrupting activity, reproductive toxicity, and genotoxicity. In this work, the feasibility of an economically and environmentally friendly catalytic system based on the thermally modified natural magnetite and hydrogen peroxide (Fe3O4-R400/H2O2), has been evaluated for the degradation of two representative sunscreen agents: benzophenone-3 (BP-3) and 4-aminobenzoic acid (PABA) in wastewater. The experiments were conducted under circumneutral pH (pH0 = 5), with temperature control (25 °C). Both compounds (500 μg L−1) were successfully removed from water by using a relatively low catalyst concentration (0.5 g L−1) and the theoretical stoichiometric H2O2 dose for their complete oxidation (∼2.3 mg L−1). Afterwards, a complete operating condition study was performed with BP-3, given its predominant occurrence in fresh waters, analysing the influence of H2O2 dose (1.2–4.6 mg L−1), catalyst concentration (0.1–0.5 g L−1), and temperature (25–45 °C). From the evolution of the identified by-products, a reaction pathway was proposed according to which oxidation of BP-3 gives rise to several aromatic intermediates, which finally evolve to short-chain organic acids. The generation of such aromatic by-products led to a considerably ecotoxicity increase in the initial stages of the reaction, but non-toxic effluents were ultimately achieved. Notably, the mineralization yield reached was above 60%. As a proof of concept, the feasibility of the system was finally demonstrated in real water matrices (WWTP effluent and surface water).

Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites.

Ulcerative colitis (UC) is a refractory disease worldwide. Liver injury can be found clinically with UC, and now, it is found that gut dysbiosis is an important mechanism in the pathogenesis of UC. Sargentodoxa cuneata has been used as a traditional Chinese medicine and is commonly used clinically for the treatment of UC. The main objective of this study was to investigate the intrinsic mechanisms of Sargentodoxa cuneata in the treatment of UC and its associated liver injuries from the perspective of intestinal flora and related metabolites. Ultra-performance liquid chromatography-mass spectrometry was used to identify the components in the aqueous extract of Sargentodoxa cuneata (AESc). Mice with UC induced by dextran sulfate sodium were used to study the effects of AESc on UC and its associated liver injuries. Furthermore, 16S rRNA gene sequencing and analysis were performed on intestinal contents, and correlation analysis of intestinal flora with short-chain fatty acids (SCFAs) and organic acids was performed. A total of 114 compounds were identified in AESc. AESc improved disease activity index scores, liver index, and colon length in mice with UC and had a good protective effect on intestine and liver injuries. Moreover, the administration of AESc regulated gut microbiota dysbiosis and the levels of a few SCFAs and organic acids in mice with UC. In addition, the correlation analysis results showed that the Megamonas and Bifidobacterium were the key intestinal flora related to the levels of differential SCFAs and organic acids in mice with UC after AESc intervention. AESc has a good protective effect on UC and UC related liver injuries. Modulation of the intestinal flora and its metabolites (SCFAs and a few organic acids) is an important pathway for AESc in the treatment of UC and also provides a rationale for the clinical use of Sargentodoxa cuneata in the treatment of UC.

Exploring the chemical diversity of Capsicum chinense cultivars using NMR-based metabolomics and machine learning methods

The habanero pepper (Capsicum chinense) is a prominent spicy fruit integral to the historical, social, cultural, and economic fabric of the Yucatan peninsula in Mexico. This study leverages the power of 1H NMR spectroscopy coupled with machine learning algorithms to dissect the metabolomic profile of eleven C. chinense cultivars, including those grown by INIFAP (Habanero-Jaguar, Antillano-HRA 1-1, Antillano-HRA 7-1, Habanero-HAm-18A, Habanero-HC-23C, and Jolokia-NJolokia-22) and commercial hybrids (Habanero-Rey Votán, Habanero-Kabal, Balam, USAPR10117, and Rey Pakal). A total of fifty metabolites, encompassing sugars, amino acids, short-chain organic acids, and nucleosides, were identified from the 1H NMR spectra. The optimized machine learning model proficiently predicted the similarity percentage between the INIFAP-grown cultivars and commercial hybrids, thereby facilitating a comprehensive comparison. Biomarkers unique to each cultivar were delineated, revealing that the Habanero-Rey Votán cultivar is characterized by the highest concentration of sugars. In contrast, the Balam cultivar is rich in amino acids and short-chain organic acids, sharing a similar metabolomic profile with the Jolokia-NJolokia-22 cultivar. The findings of this study underscore the efficacy and reliability of NMR-based metabolomics as a robust tool for differentiating C. chinense cultivars based on their intricate chemical profiles. This approach not only contributes to the scientific understanding of the metabolomic diversity among habanero peppers but also holds potential implications for food science, agriculture, and the culinary arts.

Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against Fusarium spp.

Legumes are indispensable crops in sustainable agricultural systems because of their capability for biological nitrogen fixation owing to symbiosis with rhizobia and soil fertility restoration. Fungal pathogens from the genera Fusarium cause rotting and wilting and produce mycotoxins in plant tissues. The use of fungicides in sustainable agricultural systems is limited; therefore, the application of biological agents with antifungal activity against Fusarium spp. is desirable. Lactic acid bacteria (LAB) are promising control agents that produce a wide spectrum of functional metabolites. Lactiplantibacillus plantarum and other lactobacilli are the most intensively studied genera of LAB in relation to antifungal activity against Fusarium spp. However, LAB strains belonging to the lactobacilli and lactococci genera have not yet been isolated and characterised from legumes. Therefore, we aimed to obtain wild strains of LAB from legumes, screen them for functional characteristics with respect to their antifungal activity, and compare their antifungal activity against isolates of Fusarium spp. from legumes. Consequently, 31 LAB isolates belonging to 10 species were obtained and identified from legumes. Their functional properties, including genetics and proteomics, short-chain organic acid production, and antifungal activity against five Fusarium spp., of Lactiplantibacillus plantarum, Lacticaseibacillus paracasei, and Lactiplantibacillus pentosus isolates, were studied. Cell-free supernatants of L. plantarum and L. pentosus showed significant suppression of mycelial growth and conidial germination.

Short and medium chain organic acids production from hydrolyzed food waste: technical–economic evaluation and insight into the product’s quality

Food waste (FW) was subjected to thermal-alkaline hydrolysis before to be used as source for organic acids (OAs) production under different temperatures and hydraulic regime in long-term acidification process. Mesophilic environment led to the highest acidification yield (0.87 g CODOAs/g VS) with chain-elongation process to caproic acid production (>9.0 g CODcap/L) occurring at lower HRT (2 days). The product’s quality, in terms of OAs production and composition, was evaluated through a multivariate approach, which indicated that the mesophilic condition was also appropriate to steadily maintain the requested product’s features.The specific gas production (SGP) from the solid-rich acidified residue was 0.47 Nm3/kg VS and, in a full-scale scenario of 150,000 kg FW/d, it can be potentially converted into 24.9 MWh/d as electrical energy. Also, according to the acidification performances, an overall OAs production of 7.2 ton/y has been estimated, generating a yearly economic outcome higher than 4,800,000 €.

Employing Spent Frying Oil as a Feedstock to Produce Short-Chain Organic Acids Using Mixed Microbial Cultures

Food industry waste and wastewater have been explored in relation to acidogenic fermentation as sources of non-competing food carbohydrates and mixed microbial cultures (MMCs), respectively, with the aim of producing short-chain organic acids (SCOAs) with general applications in polyhydroxyalkanoates (PHAs) production. However, studies on acidogenic fermentation using lipidic substrates are scarce. In this work, it was hypothesized that spent frying oil (SFO) could be used as a substrate for SCOA production via MMCs. In this study, oleic acid was used as a model molecule. The characterization of SFO revealed that it is mainly composed of oleic acid (81%), with minor amounts of palmitic, linoleic, and stearic acids. Different MMCs and food-to-microorganism (F/M) ratios were tested. MMCs collected in the aerobic tank of a municipal wastewater treatment plant (AES), at a 1:1 F/M, allowed to obtain the highest SCOA concentration (1.50 g COD/L) and the most diverse profile of SCOAs, with the production of acetic, propionic, butyric, iso-butyric, and valeric acids at 48:17:9:13:13% on a molar basis, respectively. This variety of odd and even SCOAs is of upmost importance, with potential applications in producing PHAs. This work can be considered a starting point for future acidogenic fermentation studies using lipid-based substrates and for the future production of PHAs.

ОЦЕНКА ЭФФЕКТИВНОСТИ ПРИМЕНЕНИЯ СИНБИОТИЧЕСКОГО КОМПЛЕКСА, СОДЕРЖАЩЕГО STREPTOCOCCUS THERMOPHILUS ЛИЗАТ, У ПОДРОСТКОВ С ВЫСОКИМ РИСКОМ КАРИЕСА

Relevance. Assessment of the risk of occurrence, prevention and treatment of caries and gingivitis in adolescents.&#x0D; Objective. To evaluate the effectiveness of the use of an oral rinse containing Streptococcus thermophilus lysate in adolescents with a high risk of caries.&#x0D; Materials and methods. The examined 25 adolescents aged 11 to 18 years used a mouthwash "Dentabalance ®" synbiotic complex for a month. The effectiveness of preventive and therapeutic measures was evaluated before and after a month of using the rinse aid by the oral hygiene index OHI-S, periodontal index PMA and indicators of mixed saliva by determining the activity of enzymes by spectrophotometric method: aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (alkaline phosphatase) in IU/l; by gas-liquid chromatography, the amount of acetic, propionic and butyric acids in mol/l.&#x0D; Results. High index values were observed in adolescents with a high risk of caries of permanent teeth: CPU (6,7), OHI-S (2,43 ± 0,07), PMA (62,1 ± 3,0%). In the mixed saliva, increased activity of enzymes was detected, the values of which were AST — 42.0 ± 8.1IU/l; LDH — 490 ± 51.2 IU/l; SCHF — 30.6 ± 1.94IU /l, the indicators of acetic and propionic acids were 2.38 ± 0.48 mol/l and 0.25 ± 0.03 mol/l, respectively, and the content of butyric acid was not determined.&#x0D; After the use of a mouthwash containing Streptococcus thermophilus lysate by adolescents, the indicators of the hygiene index OHI-S decreased statistically significantly (p &lt; 0.05) by 54.1% and the values of the PMA index by 53.3%; in mixed saliva, a decrease in the activity of AST was determined by 3.5 times, LDH by 4.7 times, alkaline phosphatase by 1.4 times, and also, the number of short-chain organic acids — acetic by 4.7 times and propionic by 1.4 times.&#x0D; Conclusions. The Dentabalance ® mouthwash is a synbiotic complex containing Streptococcus thermophilus lysate, has an anti-carious and anti-inflammatory effect, which allows it to be recommended for use by adolescents with a high risk of caries and gingivitis.Relevance: Due to the high prevalence of caries and inflammatory lesions of periodontal tissues in adolescents, the assessment of the risk of occurrence, prevention and treatment of these diseases remains relevant.

Modelling the Effect of Water Removal by Reverse Osmosis on the Distillation of Mixtures of Short-Chain Organic Acids from Anaerobic Fermentation

Anaerobic fermentation (AF) to produce sustainable short-chain organic acids (SCOAs) has found no commercial application so far. This is due to several limitations, including the high energy consumption of the SCOAs’ separation from water by distillation. This study used AspenPlus simulations to investigate the benefits of reverse osmosis (RO) to remove water and concentrate the SCOAs from AF before their separation by distillation. The effect of RO on distillation reflux ratio, heat energy requirements, column diameter and equipment costs was simulated for the processing of model SCOA-containing streams, representing AF effluents. A total of 90 simulations were carried out, investigating three different SCOA compositions, corresponding to different ratios of lactic, acetic and propionic acids, three different concentrations of the total SCOAs (10, 50, 100 g/kg in the stream entering RO) and different extents of water removal by RO. RO brought a reduction in the distillation reboilers’ duty of up to more than 90%, with a reduction of column diameter of up to more than 70%. The total energy consumption, equipment cost and NPV (net present value) of the RO plus distillation process were in all cases more favourable than for the process without membranes.

Depicting the landscape of gut microbial-metabolic interaction and microbial-host immune heterogeneity in deficient and proficient DNA mismatch repair colorectal cancers

BackgroundAccumulating evidence has indicated the role of gut microbiota in remodeling host immune signatures, but various interplays underlying colorectal cancers (CRC) with deficient DNA mismatch repair (dMMR) and proficient DNA...

How does the pattern of root metabolites regulating beneficial microorganisms change with different grazing pressures?

Grazing disturbance can change the structure of plant rhizosphere microbial communities and thereby alter the feedback to promote plant growth or induce plant defenses. However, little is known about how such changes occur and vary under different grazing pressures or the roles of root metabolites in altering the composition of rhizosphere microbial communities. In this study, the effects of different grazing pressures on the composition of microbial communities were investigated, and the mechanisms by which different grazing pressures changed rhizosphere microbiomes were explored with metabolomics. Grazing changed composition, functions, and co-expression networks of microbial communities. Under light grazing (LG), some saprophytic fungi, such as Lentinus sp., Ramichloridium sp., Ascobolus sp. and Hyphoderma sp., were significantly enriched, whereas under heavy grazing (HG), potentially beneficial rhizobacteria, such as Stenotrophomonas sp., Microbacterium sp., and Lysobacter sp., were significantly enriched. The beneficial mycorrhizal fungus Schizothecium sp. was significantly enriched in both LG and HG. Moreover, all enriched beneficial microorganisms were positively correlated with root metabolites, including amino acids (AAs), short-chain organic acids (SCOAs), and alkaloids. This suggests that these significantly enriched rhizosphere microbial changes may be caused by these differential root metabolites. Under LG, it is inferred that root metabolites, especially AAs such as L-Histidine, may regulate specific saprophytic fungi to participate in material transformations and the energy cycle and promote plant growth. Furthermore, to help alleviate the stress of HG and improve plant defenses, it is inferred that the root system actively regulates the synthesis of these root metabolites such as AAs, SCOAs, and alkaloids under grazing interference, and then secretes them to promote the growth of some specific plant growth-promoting rhizobacteria and fungi. To summarize, grasses can regulate beneficial microorganisms by changing root metabolites composition, and the response strategies vary under different grazing pressure in typical grassland ecosystems.