Event Abstract Back to Event Effect of febrile seizures on newborn hippocampal dentate granule cell morphology Marjolein Raijmakers1, 2*, Elke Clynen1, Nick Smisdom1, 3, Sofie Nelissen1, Bert Brône1, Jean-Michel Rigo1, Govert Hoogland2 and Ann Swijsen1 1 Hasselt University, Biomedical Research Institute BIOMED, Belgium 2 Maastricht University Medical Center, School for Mental Health and Neuroscience, Netherlands 3 Flemish Institute for Technological Research, Environmental Risk and Health Unit, Belgium Febrile seizures (FS) are a risk factor for the development of temporal lobe epilepsy in later life. Previously, we have shown that experimental FS alter inhibitory synaptic input and postsynaptic GABA(A) receptor function of dentate granule cells (DGC). Moreover, we showed that the survival of DGC, born after seizures, increased. Newborn DGC are therefore hypothesized to contribute to the development of a hyperexcitable hippocampal network and to play a role in the development of temporal lobe epilepsy in later life. In this study, we analyzed structural integration of newborn DGC into the existing hippocampal network. We used an established model where FS are induced in 10-day old rat pups by subjecting them to heated air. Newborn DG cells were labelled one day after treatment by a stereotactic injection of eGFP expressing retroviral particles in the dentate gyrus. After one, four and eight weeks, confocal Z-stacks were acquired allowing morphological analyses of GFP positive DG cells. Preliminary results show that one week after FS, dendritic length is significantly increased compared to controls. Moreover, four and eight weeks after seizures, Sholl analysis revealed that dendritic complexity is increased in FS animals. From these data we conclude that experimental FS can alter dendritogenesis in post-FS born DG cells. These changes are expected to result in an increased connectivity of the hippocampal network. Acknowledgements This research was financially supported by a ‘Bijzonder Onderzoeksfonds’ grant from Hasselt University. Keywords: febrile seizures, Neurogenesis, Dentate Gyrus, Epilepsy, Temporal Lobe, integration Conference: 11th National Congress of the Belgian Society for Neuroscience, Mons, Belgium, 22 May - 22 May, 2015. Presentation Type: Oral or Poster presentation Topic: Neuroscience Citation: Raijmakers M, Clynen E, Smisdom N, Nelissen S, Brône B, Rigo J, Hoogland G and Swijsen A (2015). Effect of febrile seizures on newborn hippocampal dentate granule cell morphology. Front. Neurosci. Conference Abstract: 11th National Congress of the Belgian Society for Neuroscience. doi: 10.3389/conf.fnins.2015.89.00039 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Apr 2015; Published Online: 05 May 2015. * Correspondence: Ms. Marjolein Raijmakers, Hasselt University, Biomedical Research Institute BIOMED, Hasselt, Belgium, Marjolein.Raijmakers@uhasselt.be Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marjolein Raijmakers Elke Clynen Nick Smisdom Sofie Nelissen Bert Brône Jean-Michel Rigo Govert Hoogland Ann Swijsen Google Marjolein Raijmakers Elke Clynen Nick Smisdom Sofie Nelissen Bert Brône Jean-Michel Rigo Govert Hoogland Ann Swijsen Google Scholar Marjolein Raijmakers Elke Clynen Nick Smisdom Sofie Nelissen Bert Brône Jean-Michel Rigo Govert Hoogland Ann Swijsen PubMed Marjolein Raijmakers Elke Clynen Nick Smisdom Sofie Nelissen Bert Brône Jean-Michel Rigo Govert Hoogland Ann Swijsen Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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