Tulsi leaf extracts has been used to produce biosynthesized copper nanoparticles. This biosynthesized nanoparticle is used for the preparation of nanofluid. The performance of nanofluid in a helical coil heat exchanger was studied for heat transfer rate and analyzed using standard correlation at the laboratory of NIT, Raichur. The fluid inlet temperature of the coil was 60 °C and temperatures of fluid inside a shell of 32 °C were maintained. A dean number in the range of 1000 – 10,000 was set on the shell side. The inlet fluid flow was constant at the coil. Thermal analysis has been carried out considering flow rate, overall heat transfer coefficient, and inlet and outlet temperature. When compared to the base fluid, using bio-synthesized Nanoparticles improved the heat transfer coefficient.