Abstract Limited studies have compared the effects of oxygen within a steak on biochemical properties. The objective was to evaluate the effects of oxygen exposure on the biochemical attributes of longissimus lumborum muscle. Steaks were (1.91 cm) sliced from USDA Low Choice beef strip loins (n = 7), packaged in polyvinyl chloride overwrap trays, and randomly assigned to day 3 or day 6 in retail display. During display, instrumental color was evaluated daily using a HunterLab MiniScan spectrophotometer. The display surface was considered as oxygen exposed (OE), while the interior of the steak was denoted as not exposed to oxygen (NOE). NOE was obtained by slicing the steak in half parallel to the previously OE surface. The NOE pieces were evaluated for bloom, while NOE and OE surfaces were used oxygen consumption (OC) and metmyoglobin reducing ability (MRA). Nitrite-induced metmyoglobin reduction was used to measure MRA, while changes in deoxymyoglobin level with vacuum were used as an indicator of OC. The data were analyzed using the GLIMMIX Procedure of SAS (n = 7 replications) and considered significance at P < 0.05. With increased display time, the a* values (redness) decreased (P < 0.05) for instrumental color. NOE steak surface had greater (P < 0.05) MRA compared with OE surfaces. Oxygen exposure affected the OC of the steaks, with the OE surfaces having lower (P < 0.05) OC compared to NOE surfaces on day 6 of display. The exposure of oxygen to the muscle resulted in a decrease in activity, paralleling in decline in retail color and color stability. In conclusion, the presence of oxygen can result in negative impacts on the shelf life of steaks; however, the non-exposed interior of muscle remains more biochemically active.