Among the most significant issues with pharmaceutical production in shared facilities is the risk of cross-contamination, which lowers product quality and costs the business a lot of money. This work set out to evaluate a cleaning approach by creating and testing a straightforward UV spectrophotometric method for estimating sildenafil citrate in swab samples. To get a good recovery from a stainless steel surface utilising a sterile cotton swab stick, the swabbing technique was fine-tuned. A wavelength of 292.20 nm was chosen for detection. Linearity, precision, accuracy, detection limit, and quantification limit were the criteria used to validate the suggested approach. A correlation coefficient of 0.9993 was determined for the regression line after studying linearity throughout a concentration range of 5 to 25 μg/mL. An average of 88.23% was recovered.