Leaf Blade Shape Research Articles

Clinopodium eplingianum (Lamiaceae), a new species from Oaxaca, Mexico

Recent botanical exploration in grasslands in northwestern Oaxaca have revealed a Clinopodium (Lamiaceae) taxon that cannot be referred to any of the current described species. This plant is most similar to C. hintoniorum from northeastern Mexico, in Nuevo Léon, in general habit and architecture; however, it can be easily distinguished by leaf blade shape and width, calyx teeth shape and length, lack of tufts of hairs between them and a distinction in the relative proportion between calyx and corolla tube. This new species represents an addition to the flora of Oaxaca and to the biogeographic province of Sierra Madre del Sur, being endemic to these two areas. The taxon is here presented as C. eplingianum in honor to Carl Epling, the most prominent scholar in the study of American Lamiaceae. Morphological description, distribution map and illustration of the species are provided.

Begonia isadorae (Begoniaceae), a new endemic species from Ilha Grande, an island in southeastern Brazil, with notes on leaf anatomy

Begonia isadorae is a new species endemic to an island of Brazilian Atlantic Rainforest, a biome rich in endemism and species diversity, and it is described and illustrated herein. Begonia isadorae belongs to B. sect Pritzelia (Klotzsch) A.DC. and it is morphologically similar to B. rubropilosa A.DC. sharing the same leaf blade shape and habit but differs in its puberulous to glabrescent adaxial leaf blades (vs. scabrous), puberulous tepals (vs. squamulose), and ovary with entire placentae (vs. bilamellate). A diagnosis, description, ecological comments, taxonomic and leaf anatomy notes, illustrations, and color photographs are provided. Begonia isadorae is preliminarily classified as Vulnerable (VU) due to its very small populations and restricted distribution.

Genetic diversity of rose apple (Syzygium samarangense) varieties based on ISSR molecular markers

Abstract. Hien PTT, Hang VTT, Cham LTT. 2024. Genetic diversity of Rose apple (Syzygium samarangense) varieties based on ISSR molecular markers. Biodiversitas 25: xxxx. Rose apple (Syzygium samarangense) exhibits a rich diversity in its varieties, metabolic compounds, and biological activities. Genetic diversity studies are crucial in understanding plant species taxonomy and evolutionary history. They provide a roadmap for researchers to trace the relationships between different varieties and offer insights into the evolutionary processes that have shaped the diversity within the species. Many traditional or indigenous varieties of plants may harbor unique traits adapted to specific local conditions. This study, therefore, holds immense value as it aims to evaluate the genetic relationship among rose apple varieties in Vietnam using morphological characteristics and ISSR markers. Sixteen local varieties of rose apple were collected from provinces in Vietnam for this purpose. The morphological characteristics of leaves and fruits were compared among samples, and the genetic diversity was evaluated using ISSR markers. The results revealed significant morphological diversity in the leaf base, blade shape, leaf color, and fruit shape and size. Based on the DNA profiles of 15 ISSR markers, 102 amplicons were produced, and the percentage of polymorphism ranged from 57 to 100%. The dendrogram constructed using the UPGMA method classified these varieties into two main clusters with a genetic diversity of 64%. Such comprehensive information underscores the genetic diversity among S. samarangense, thereby contributing to the germplasm resources for future plant breeding programs.

Morphological and genetic characterization of jackfruit (Artocarpus heterophyllus) in the Kayunga and Luwero districts of Uganda

BackgroundJackfruit (Artocarpus heterophyllus) is an economically valuable fruit tree in Uganda. However, the production of jackfruit in Uganda is low. Additionally, because of deforestation, genetic erosion of the resource is predicted before its exploitation for crop improvement and conservation. As a prerequisite for crop improvement and conservation, 100 A. heterophyllus tree isolates from the Kayunga and Luwero districts in Uganda were characterized using 16 morphological and 10 microsatellite markers.ResultsThe results from the morphological analysis revealed variations in tree height, diameter at breast height (DBH), and crown diameter, with coefficient of variation (CV) values of 20%, 41%, and 33%, respectively. Apart from the pulp taste, variation was also observed in qualitative traits, including tree vigor, trunk surface, branching density, tree growth habit, crown shape, leaf blade shape, fruit shape, fruit surface, flake shape, flake color, flake flavor and pulp consistency/texture. Genotyping revealed that the number of alleles amplified per microsatellite locus ranged from 2 to 5, with an average of 2.90 and a total of 29. The mean observed (Ho) and expected (He) heterozygosity were 0.71 and 0.57, respectively. Analysis of molecular variance (AMOVA) indicated that 81% of the variation occurred within individual trees, 19% among trees within populations and 0% between the two populations. The gene flow (Nm) in the two populations was 88.72. The results from the ‘partitioning around medoids’ (PAM), principal coordinate analysis (PCoA) and genetic cluster analysis further revealed no differentiation of the jackfruit populations. The Mantel test revealed a negligible correlation between the morphological and genetic distances.ConclusionsBoth morphological and genetic analyses revealed variation in jackfruit within a single interbreeding population. This diversity can be exploited to establish breeding and conservation strategies to increase the production of jackfruit and hence boost farmers’ incomes. However, selecting germplasm based on morphology alone may be misleading.

Assessing the risk and morphological characteristics of weedy rice outbreak in Thap Muoi district, Dong Thap province, Vietnam

The Thap Muoi district in Dong Thap province, Vietnam, is renowned as the largest rice producer, cultivating 112,762 hectares and specialising in three rice crops per year, with some areas achieving 3.5 annual crops. However, changing farming practices, low-quality rice seeds, and mechanisation have led to the emergence and spread of weedy rice (Oryza spp.) in Thap Muoi and the broader Mekong delta. This comprehensive assessment of weedy rice outbreak risks in Thap Muoi provides a vital foundation for effective management strategies to prevent its spread in the Mekong delta. Sixty questionnaires were collected from rice-farming households. Approximately 80% of farmers reported the appearance of weedy rice approximately five years ago. Alarmingly, 95% of surveyed farmers (57 individuals) reported weedy rice infestations, with 40% of them encountering infection levels ranging from 11 to 20 plants/m2. To distinguish weedy rice from cultivated varieties, seven distinct types were identified based on various morphological characteristics, including growth time, leaf blade structure, plant height, leaf/stem colour and shape, grain beard/tail, seed/husk colour, grain length, ripening time, and seed dispersal potential. However, all seven weedy rice varieties exhibited short growth cycles and the capacity to produce seeds, posing significant challenges to rice production management in Thap Muoi. This research underscores the critical importance of assessing weedy rice outbreak risks in Thap Muoi and understanding its morphology and prevalence. This understanding is essential for developing effective preventive measures to mitigate the threat of weedy rice outbreaks across the entire Mekong delta in Vietnam.

Genetic diversity of kale (Brassica oleracea L. var acephala) using agro-morphological and simple sequence repeat (SSR) markers

Kale (Brassica oleracea. var. acephala) is a nutrient-rich green leafy vegetable consumed as food and used in traditional medicine worldwide. An essential step in describing the available genetic resources and ensuring their effective use in breeding programs is to characterize the genetic diversity of available germplasm. In this study, the genetic diversity and structure of 26 kale accessions from South-East Europe were examined using 26 agro-morphological traits collected in the field and 12 simple sequence repeat (SSR) markers. Considerable agro-morphological variability was found in most quantitative (CV = 17.26–42.42%) and qualitative (H' = 0.61–1.79) traits. Multifactorial analysis (MFA) showed that country of origin (33.01%) and morphotype (32.30%) significantly influenced kale diversification. Leaf blade shape (20.62%), leaf incision (19.43%), anthocyanin distribution (16.43%), and leaf colour (15.55%) were the traits that most clearly differentiated accessions. The three common commercial kale cultivars were identified as independent outliers that differed from the other kale accessions in both MFA and UPGMA clustering analysis. The SSR markers were highly informative with 108 alleles and polymorphic information content ranging from 0.395 to 0.856. Strong genetic diversity was detected at the accession level (H' = 0.58) while genetic differentiation was low (Fst = 0.05). Similar to UPGMA clustering, Bayesian clustering suggests that the kale collection can be divided into four clusters with a high degree of admixture and no geographic grouping pattern is apparent. Overall, the study showed that the kale collection studied represents a valuable reservoir of genetic and agro-morphological variability that could be used for future breeding initiatives.

Oreocharis chenzhouensis, a new species of Gesneriaceae from southern Hunan, China

Oreocharis chenzhouensis, a new species, is described and illustrated from southern Hunan, China. The new species resembles O. burtii and O. sinohenryi in leaf blade shape and indumentum of the plants, but readily differs from the latter two in its leaf blade with a cuneate and usually asymmetric base, abaxially glabrous corolla tube, narrowly triangular calyx lobes, and one or no staminode. Following the IUCN Red List Criteria, O. chenzhouensis is assessed as “Vulnerable”.

Metapetrocosmea alba (Gesneriaceae), a new species from central Vietnam

Based on morphological comparison and literature consultation, a new species, Metapetrocosmea alba (Gesneriaceae), is confirmed from Bach Ma National Park, central Vietnam. This new species is similar to M. poilanei, but differs by its longer and white funnelform corolla and three staminodes. And it is easy to distinguish the new species from M. cycnostyla by its distinct stem, and the shape and size of leaf blade. A detailed morphological description and photographic illustration of the new species are provided along with ecological information and geographic distribution.

Studi Morfologi Genus Piper dan Variasinya

The Piper genus is a member of the piperaceae family which is spread throughout the tropics and subtropics from the lowlands to the highlands, including Indonesia. Piper is an important spice and phytopharmaca plant that is used by the Indonesian people for their needs for spices and traditional medicine. This research was conducted with the aim of knowing the morphological variations of plants belonging to the genus Piper found in Banyumas, Purbalingga, and Banjarnegara regencies. A total of 30 individuals belonging to the genus Piper were observed using 39 morphological characters. The method used is a survey method with a purposive random sampling technique. The data obtained from morphological observations were analyzed descriptively to obtain morphological variations of members of the genus Piper. The results of research in Banyumas, Purbalingga and Banjarnegara regencies found 6 plants belonging to the Piper genus, namely red betel (Piper crocatum Ruiz & Pav.), green betel (Piper betle L.), yellow betel (Piper sp.), pepper (Piper nigrum L. .), cubeb (Piper cubeba L.f.), Javanese chili (Piper retrofractum Vahl). Variations in morphological characters of 6 plants belonging to the genus Piper were found in plant height, stem color, distance between internodes on the stem, leaf blade shape, leaf blade tip, leaf base. leaf color, color of the upper surface of the leaf, color of the lower surface of the leaf, length of the leaf blade, width of the leaf blade, texture of the upper and lower surface of the leaf, texture of the petiole, length of the petiole, color of the petiole, and aroma on the leaves.

Polar auxin transport modulates early leaf flattening

The flattened leaf form is an important adaptation for efficient photosynthesis, and the developmental process of flattened leaves has been intensively studied. Classic microsurgery studies in potato and tomato suggest that the shoot apical meristem (SAM) communicates with the leaf primordia to promote leaf blade formation. More recently, it was found that polar auxin transport (PAT) could mediate this communication. However, it is unclear how the expression of leaf patterning genes is tailored by PAT routes originating from SAM. By combining experimental observations and computer model simulations, we show that microsurgical incisions and local inhibition of PAT in tomato interfere with auxin transport toward the leaf margins, reducing auxin response levels and altering the leaf blade shape. Importantly, oval auxin responses result in the bipolar expression of SlLAM1 that determines leaf blade formation. Furthermore, wounding caused by incisions promotes degradation of SlREV, a known regulator of leaf polarity. Additionally, computer simulations suggest that local auxin biosynthesis in early leaf primordia could remove necessity for external auxin supply originating from SAM, potentially explaining differences between species. Together, our findings establish how PAT near emerging leaf primordia determines spatial auxin patterning and refines SlLAM1 expression in the leaf margins to guide leaf flattening.

Oreocharisqianyuensis, a new species of Gesneriaceae from Southwest, China based on morphological and molecular evidence.

Oreocharisqianyuensis, a new species of Gesneriaceae from Southwest, China, is described and illustrated based on morphological comparisons and molecular phylogenetic analyses. Phylotranscriptomic analyses of the new species in the context of a comprehensive phylogeny with dense sampling of 88% (111/126) of all species of the genus indicated that the new species was most closely-related to O.fargesii. The new species is morphologically similar to O.fargesii and O.nanchuanica in the shape, color and structure of flowers and the number of stamens, but differs in the leaf blade shape, margin and the indumentum characters of the inflorescence. Its morphological relationship with similar species is discussed, the detailed descriptions, colour photographs, distribution, as well as the IUCN threatened status based on the IUCN Red List Categories and Criteria are also provided.

Three Brazilian species of Begonia (Begoniaceae) from the state of Rio de Janeiro, Brazil

Begonia altimontana, B. inumbrata and B. pedrabrancencis (Begoniaceae) are species endemic to the Brazilian Atlantic Coastal Forest, an area rich in endemism and species diversity, and are described and illustrated herein. Begonia altimontana resembles B. olsoniae L.B.Sm. & B.G.Schub., B. princeps A.DC. and B. ramentacea Paxton in the shape of leaf blades and squamulose petioles, and is found in the same habit (rocky slopes and outcrops). Begonia inumbrata resembles B. fluminensis Brade in the shape of the leaf blades and fruit, and B. pedrabrancencis is morphological similar to B. bidentata Raddi sharing the same shape of the leaf blades and venation. We place both new species in Begonia sect. Pritzelia, based on their similarity to other species in the section, however this section is highly polymorphic, and we highlight that further work is needed on sectional placement. The lectotype of Begonia bidentata Raddi is designated here and we also lectotypified B. ramentacea Paxton using an illustration and designated an epitype.

The methodology for assessing an integral indicator of the degree of favorable weather conditions for plants

The weather and climatic conditions of the periods under study significantly affect the response of biological systems to other environmental factors, including technogenic transformation of the environment. Having a complex of weather factors, it is difficult to unequivocally judge the favorable weather conditions of a particular year of observation. In this paper, an integral indicator of the degree of favorable weather conditions and a technology for its calculation are proposed. The degree of favorable weather is expressed in points on a five-point scale. The calculation uses a set of weather factors that potentially affect the biological object under study (organism, population, community). Weather factors and periods in which they can affect the objects under study are selected taking into account the biological characteristics of the object, for example, in our case, the period of the intrarenal phase (May of the previous year), the stage of active organogenesis, extrarenal growth until the period of collection of biological material (July of the current year). The method of principal components reduces hydrometeorological indicators and determines the factors that make the greatest contribution to weather variability in the periods under study. The absolute values of these factors are converted into points on the basis of a five-point scale for the correspondence of weather factors to the climatic norm and the optimal values of the HTC. Based on the average score for each year, the degree of favorable weather for a particular biological object is determined. The paper contains an example of a technology for calculating the integral indicator of favorable weather for silver birch (Betula pendula Roth), growing in six localities of the Tagil zone of the Middle Urals, in which the variability of leaf morphological features was studied in the period from 2016 to 2019. On the example of the Betula pendula leaf blade shape index, a statistically significant dependence of this feature on the integral indicator of the degree of favorable weather is shown.

Morphological and biological features of species of the genus Echinacea Moench during introduction to the Southern coast of the Crimea

The paper presents the results of an introduction study of four species of the genus Echinacea Moench from the collection of aromatic and medical plants of the Nikitsky Botanical Garden: E. angustifolia DС. , E. pallida (Nutt.) Nutt. , E. tennesseensis (Breadle) Small and E. purpurea (L.) Moench. Morphological and biological features of these species were identified in order to determine the prospects of their cultivation in the conditions of the Southern Coast of the Crimea. A positive interspecific dependence of the number of inflorescences (baskets) on a plant with the length of the growing season (r=0.92) was established. An intraspecific assessment of the morphometry of inflorescences, such as their diameter, the length of the lingual flowers and the height of the flower bed, showed that E. purpurea has a tendency to negatively correlate these indicators with their number on the plant. Among the studied species of the genus Echinacea, according to the complex of thirteen morpho-biological and economically valuable traits, two species differ in adaptive advantage - these are E. purpurea and E. tennesseensis , and the best samples are No. 15309 and No. 6106 with an aboveground mass yield, respectively, 1.20±0.21 kg/plant and 0.92±0.19 kg/plant. E. pallida is characterized by cumulative signs of drought resistance, it is a short growing season equal to 124±4.1 days and a narrow-lanceolate leaf blade shape (index - 9.6), which allows this species to form a significant yield of aboveground mass (0.86±0.17 kg/plant) in the arid conditions of the subtropical climate of the Southern coast of the Crimea.

Анализ изменчивости морфологических признаков сортов камелии японской (Camellia japonica L.) в условиях защищенного грунта г. Москвы

In the present work, the analysis of the variability of quantitative and qualitative morphological traits of Japanese Camellia (Camellia japonica L.) varieties used in greenhouses of botanical institutions in Moscow is considered. The paper considers the following varieties: ‘Governor Mouton’, ‘Madame Lebois’, ‘Covina’, ‘Bella Toscana’, ‘Bonomiana’, ‘Alba Simplex’, ‘Lavinia Maggi’, ‘ Silver Waves ’,‘ Doncklaeri ’. To assess the variability, 10 characters were selected, six of which describe the leaf (leaf blade length, leaf blade width, leaf ovality index, leaf blade base shape, leaf blade apex shape, leaf blade shape) and four – flowers (corolla color, flower diameter, degree of terry and aroma). The variability of quantitative characteristics was assessed by determining the confidence intervals method and calculating the coefficients of variation. The variability of qualitative features was assessed by establishing modalities and determining modes. It is noted that, in terms of quantitative and qualitative characteristics, weak variability is observed in most cases. Thus, the studied varieties of Japanese camellia have stable features that characterize the flower and leaf blade. Keywords: CAMELLIA JAPONICA, JAPANESE CAMELLIA, VARIABILITY, VARIETIES

Species Delimitation of Asteropyrum (Ranunculaceae) Based on Morphological, Molecular, and Ecological Variation.

Objectively evaluating different lines of evidence within a formalized framework is the most efficient and theoretically grounded approach for defining robust species hypotheses. Asteropyrum Drumm. et Hutch. is a small genus of perennial herb containing two species, A. cavaleriei and A. peltatum. The distinction of these two species mainly lies in the shape and size of leaf blades. However, these characters have been considered labile and could not differentiate the two species reliably. In this study, we investigated the variation of the leaf blades of 28 populations across the whole range of Asteropyrum using the landmark-based geometric morphometrics (GMM), sought genetic gaps within this genus using DNA barcoding, phylogenetic reconstruction and population genetic methods, and compared the predicted ecological niches of the two species. The results showed that the leaf form (shape and size) was overlapped between the two species; barcode gap was not detected within the genus Asteropyrum; and little ecological and geographical differentiation was found between the two taxa. Two genetic clusters detected by population genetic analysis did not match the two morphospecies. The results suggest that there are no distinct boundaries between the two species of Asteropyrum in terms of morphology, genetics and ecology and this present classification should be abandoned. We anticipate that range-wide population genomic studies would properly delineate the species boundaries and help to understand the evolution and speciation within Asteropyrum.

Diversity of Morphologically Vegetative Characters of Kepel (Stelechocarpus burahol): Study in Yogyakarta Palace Region, Indonesia

<p class='IJASEITAbtract'>Kepel or <em>Stelechocarpus burahol</em> is a plant member of the <em>Annonaceae</em> family that is very useful as herbal medicines and natural deodorant for the Javanese palace’s monarch. Kepel is an endemic Javanese plant that is currently very rare and endangered, so preservation and conservation are essential. The study aims to characterize vegetative morphology and determine the clustering of Kepel in Yogyakarta Palace Region as the first step for Kepel conservation. The study was carried out by a survey method using purposive sampling in 14 regions in Yogyakarta Palace Region. The plant used as an accession was healthy, grows normally, and has produced fruits. The variable observation of the canopy, stem, and leaf refers to the <em>Annonaceae</em> family’s description based on Bioversity International. Data analysis is performed using cluster analysis based on the coefficient of similarity. The results showed that 46 <em>kepels </em>in Yogyakarta Palace Region had a high diversity based on the analysis of similarities in 16 variables with a similarity coefficient of 0.5-0.91. Analysis of cluster described that there are two clusters with a coefficient similarity of 0.5. Cluster A has 40 accessions (87%); cluster B has six accessions (13%). Leaf shape, leaf size, and stem color have an essential role in clustering the Kepel in Yogyakarta Palace Region. Cluster A relatively has a smaller leaf than the leaf of cluster B. The leaf blade shape of cluster A is elliptic to lanceolate, and the stem colors are beige-green and grey. Cluster B has an elliptical to ovate leaf blade shape, and the stem color is light brown, brown, and reddish-brown. This research data can enrich Kepel germplasm information, which is very useful as basic data for the conservation, development, and breeding of Kepel plants.

Features of Trifolium rubens (Leguminosae) ontomorphogenesis in conditions of Ukraine

The necessary basis for studying the structure and dynamics of populations is an investigation of ontomophogenetic peculiarities and establishment of ontogenetic stages of individuals. Information about ontomorphogenesis is especially important for protected species of plants. The minimal amount of data on morphology of plants in the different periods of ontogenesis complicates plant identification and evaluation of population state conditions in nature and culture. Trifolium rubens L. is a perennial Central European plant listed in the Red Book of Ukraine as a rare species. The objective of this research is to analyze the peculiarities of ontomorphogenesis of T. rubens, to establish diagnostic signs of age stages and the sequence of their passage for identification of the multivariance of development. The results of the study on T. rubens ontomorphogeny at the M. M. Gryshko National Botanical Garden оf the National Academy of Sciences of Ukraine and in the natural population in Zakarpattya are represented in this article. The terms “age stage” and “age period” are used to describe the degree of ontogenetic development of individuals. Diagnostic features of the age periods: latent, pregenerative, generative and senile are established. Detailed morphological descriptions of plants in different stages of ontogenesis are presented. The study shows a multivariate development that reveals different sequences of passing ontogenesis stages by individuals and, accordingly, the rate of their aging and life expectancy. It was found that during the first year of vegetation the plants undergo two periods (latent and pregenerative) and 5 stages of ontogeny (latent, seedlings, juvenile, immature and virginile). The first bloom in plants appears mainly in the second year of vegetation. The research showed the change of the leaf blade shape in ontogeny from simple to compound. There is a difference in the pubescence of leaf parts, and leaf shape indicates that these features are age-old. It was concluded that the aboveground part of the immature, virginile, generative individuals during the flowering period of plants differ from subsenile and senile in shape and number of leaves. In the generative period of ontogeny in dense plantings or in the natural population, it is difficult to reliably determine the boundaries of an individual by the aboveground part. The correlation between vegetative shoots of different type and generative shoots in the individual or group of plants can be used as an indicator to estimate the ontogenetic status of a particular locus or population. Successful growth of T. rubens in culture outside the natural range indicates good prospects for its ex situ protection and the implementation of measures for repatriation and restoration of populations in nature. The data of the research may be used for evaluation of the age structure of T. rubens populations.

Rhododendron kuomeianum (Ericaceae), a new species from northeastern Yunnan (China), based on morphological and genomic data

Rhododendron kuomeianum Y.H. Chang, J. Nielsen & Y.P. Ma, a new species of Rhododendron (Ericaceae) within subsect. Maddenia in sect. Rhododendron from Yiliang County, NE Yunnan, China, is described and illustrated. The new species is similar to R. valentinianum, but it can be easily distinguished by its sparse scales on the abaxial surface of the leaf blade, fewer flowers per inflorescence and white corolla with pale red margins. There are also differences in the widths of calyx lobes, leaf blade shape and indumentum characteristics of the petiole between the new species and Rhododendron linearilobum. We confirmed that R. kuomeianum is a new species closely related to R. valentinianum and R. changii with phylogenomic studies of 10 species within this subsection based on restriction site-associated DNA sequencing (RAD-seq) data. These phylogenomic analyses also clarified additional taxonomic problems in this subsection previously raised by morphological analysis. Our findings make a strong case for using next-generation sequencing to explore phylogenetic relationships and identify new species, especially in plants groups with complicated taxonomic problems.

Morphological and genetic polymorphism of the introduced population of Prunus pumilaL. in Chelyabinsk Province

Background.Studying genetic polymorphism of the Prunus pumilaL. representatives with a wide range of variability in morphological characters will make it possible to identify promising genotypes with high combining ability for hybridization.Material and methods.The study covered 39 genotypes from the Chelyabinsk cultivated population and 6 genotypes from Khakassia, with extreme or intermediate expressions of such characters as fruit size, color and shape; stone shape and surface structure; leaf blade serration and shape; plant habitus, and some others. Genetic distances were calculated using the data matrix for binary states of presence (1) or absence (0) of amplified fragments with the Past Program (Gower). The UPGMA dendrograms were obtained in TREECON (version 1.3b).Results and discussion. The selected 5 ISSR and 2 RAPD primers demonstrated their effectiveness in detecting genetic polymorphism in the studied accessions. In individual cases, a weak connection of separate markers with leaf blade edge serration, stone surface structure, plant habitus, and geographic origin of the genotype was detected. Concurrent inclusion of all primers and genotypes in the cluster analysis failed to group the studied P. pumilasamples into clades according to the presence/absence of any traits, which may attest to their close genetic affinity. An independent clade with reliable bootstrap support (100%) was formed only for the genotypes of the taxonomically close sp. P. tomentosaThunb., analyzed as an external group. Moreover, this clade manifested (with the 86% reliability of bootstrap support) differences between the hybrid genotypes of P. pumila× P. tomentosa(F3) and the genotype of P. tomentosa. Parent pairs promising for fruit quality and shrub shape improvement through breeding were identified using the produced indices of genetic distinction.