The aim of the study was to evaluate the skull shape of Wistar albino rats using the geometric morphometry method. For this purpose, a total of 52 adult rat skulls, 31 female and 21 male, were evaluated. Skulls were photographed from equal distance in dorsal and ventral directions. 13 and 18 landmarks were marked in dorsal and ventral images, respectively. Percentage variation values of principal components were calculated by performing principal component analysis in the MorphoJ program. Average shape graphs showing the positive and negative boundaries of the first three principal components were obtained. It was defined that scatter plot graphic showing the degree of grouping of male and female rats according to the first and second principal component. The study also examined the allometric effect with regression. Discriminant function analysis was performed to examine gender dimorphism. As a result of the study, it was determined that the most significant shape change was in the zygomatic arc and palate regions according to individuals, while it was also in the neurocranium acording to gender. We believe that the study will contribute to anatomy, biology and archeology studies on the shape of skull.