Shallot Cultivars Research Articles

Comparative metabolite profiling in single and multiple bulb cultivars of shallot (Allium cepa L. Aggregatum group)

Tuk-Tuk and Sanren F1 are two shallot cultivars that exhibit divergent bulb multiplication traits. Tuk-Tuk typically produces one bulb. In contrast, Sanren F1 tends to produce multiple bulbs. This study used metabolomics during bulb development and harvesting. The objective of the study was to identify the metabolites that might affect the bulb multiplication of shallot. The initial samples of both cultivars were collected during the bulbs developmental stage i.e, 11 weeks after sowing (WAS), while the second sample was gathered during harvesting stage (16 WAS). The Proton Nuclear Magnetic Resonance (1H NMR) analysis was applied to freeze-dried shallot bulbs formetabolomics analysis. Multivariate analysis utilizing Principal Component Analysis (PCA) followed by Orthogonal Projections Discriminant Analysis (OPLS-DA) were conducted using MetaboAnalyst 5.0. The 1H NMR spectra revealed 28 putative metabolites. All data, including both cultivars during development and harvesting, was not clearly separated by PCA. However in the development stage, Sanren F1 was observed to have higher histidine than Tuk-Tuk, indicating the involvement of histidine kinase signaling system in the bulb multiplication. Hence, histidine kinase known for its role in the cytokinin signaling which contributing in the cell division regulation and may affect Sanren F1 bulb multiplication. Four metabolites i.e., sucrose, arginine, acetic acid, and myricetin were higher in Tuk-Tuk than Sanren F1, a cultivar tends to produce a single bulb. Valine, phenylalanine, formic acid, and α-linolenic acid were considerably higher in Sanren F1 than Tuk-Tuk during harvesting. There were also several metabolites with greater relative concentrations than the others. Though not all of those metabolites significantly differed amongst the examined cultivars, it was thought that they had an impact on bulb development. It would be of great interest to ascertain whether metabolites detected in Sanren F1 at higher levels have the potential to function as chemical markers for distinguishing shallot cultivars that are capable of producing multiple bulbs.

Antibacterial activity of Shallots (Allium xwakegi Araki.) cultivars in Palu Valley against Salmonella Typhi ATCC 27870 through in vitro and in silico evaluation

Background and Objectives: Shallots, recognized for their minimal toxicity, cost-effectiveness, and widespread avail- ability, are increasingly considered a viable source of biological activity. This study evaluates the antibacterial efficacy of a specific shallot cultivar from Palu Valley, Indonesia, against Salmonella typhi, the pathogen responsible for typhoid fever. Materials and Methods: Utilizing thin-layer chromatography (TLC-bioautography) and gas chromatography-mass spec- troscopy (GC-MS), the study identifies active compounds in shallot ethanol extract and employs molecular docking to assess interactions between receptors and ligands. Results: Findings indicate significant antibacterial activity, with a notable inhibition zone diameter of 31.5 mm at spot Rf 0.28 in TLC bioautography and an optimum concentration of 2% yielding an average clear zone diameter of 28.27 mm in the agar diffusion test. GC-MS analysis reveals 41 compounds, predominantly dodecanoic acid and 1,2,3-propanetriyl ester. Ad- ditionally, molecular docking reveals the lowest binding affinity (-7.3 kcal/mol) for Ergost-8-En-3-Ol, 14-Methyl-, (3.Beta,5. Alpha.) against DNA gyrase. Conclusion: This study confirms Palu Valley shallot extract's potent antibacterial effect against Salmonella typhi, highlight- ing its therapeutic potential.

Growth, yield, and secondary metabolite responses of three shallot cultivars at different watering intervals

Abstract Indonesia is a significant producer of shallots, and the country frequently cultivates three distinct varieties: Bima, Trisula, and Sumenep. Each cultivar exhibits distinct characteristics, specifically when grown under suboptimal conditions. Several studies showed that insufficient watering intervals (WIs) can lead to drought stress, while excessive levels often lead to inundation. To investigate the effects of cultivar and WI on shallot growth and quality, this study employed a split-plot design with main plots and subplots with four repetitions. The main plots consisted of cultivars (Bima, Trisula, and Sumenep), while the subplots comprised WIs (once in 1 day, 2 days, and 3 days). The results showed that there was an effect of interaction between cultivars and WIs on growth (proline [126.15 mg 100 g−1]) and quality (quercetin [3.8739 mg g−1], rutin [0.2080 mg g−1], and kaempferol [1.209 mg g−1]). However, there was no effect on the number of tillers, shoot/root ratio, water content, number of tubers, tuber weight, and total flavonoid.

Effect of Fobio on Intensity of Moler Disease (Fusarium oxysporum) on Various Shallot Cultivars

Fusarium oxysporum is the pathogen that causes moler disease which can reduce the productivity of shallot plants. The use of cultivars greatly affects the production. Control method that is more environmentally friendly is currently being pursued towards organic farming. Fobio is a biopesticide containing plant root microorganisms as biological control agents. This study aims to determine the effect of Fobio biopesticide on Fusarium wilt (moler) disease incidence and production of three shallot cultivars. The experiment was conducted in the shallot planting area of Sukorejo Village, Rejoso District, Nganjuk Regency, East Java. This experiment was arranged in the split plot design with two factors. The main plot of the concentration of the biopesticide formula (Fobio) consisted of 3 levels namely control (chemical fungicide), 5 ml/liter, and 10 ml/liter, and the subplot of shallot cultivar consisted of 3 types namely Tajuk, Biru Lanchor, and Super Philip. The observation in this study were the incubation period, disease symptoms, disease intensity, and shallot bulb yield. The results showed that Fobio biopesticide at the concentration of 10 ml/liter caused the same disease suppression as chemical fungicide (control) in the three shallot cultivars with the disease incidence of 18.78%. Fobio biopesticide concentration of 10 ml/liter had also the same effect as chemical fungicide on the production of those shallot cultivars, with the average wet weight of 2.25 kg/treatment and dry weight of 1.49 kg/treatment.

BREEDING VALUE OF SHALLOT CULTIVARS AND LOCAL FORMS IN TERMS OF ECONOMIC CHARACTERS FOR THE RIGHT-BANK FOREST-STEPPE OF UKRAINE

Purpose. To indentify the best shallot cultivars and local forms by growing period length, bulb yield, leaf productivity, and bulb weight and number per clump in the Right-Bank Forest-Steppe of Ukraine. Methods. Statistical processing, field assessments, computational and analytical methods. Results. Collection shallot accessions were evaluated for growing period lengthand, bulb weight, number and performance. Earlyripening accession ‘Sh-8’ with a vegetation period of 74 days was selected. Local form ‘Sh-1’ from the Kyivska Oblast region turned out to be the best in terms of the mean bulb weight per clump (26.7 g). Accessions from Kyivska (‘Sh-1’, ‘Sh-2’), Dnipropetrovska (‘Sh-6’, ‘Sh-9’), and Chernihivska (‘Sh-10’) Oblasts yielded the most (30.5, 25.4, 18.9, 18.1, and 19.4 t/ha, respectively). The check cultivar, ‘Lira’, yielded 15.5 t/ha. The smallest yields were harvested from the following accessions: ‘Sh-4’ (12.2 t/ha), ‘Sh-7’ (12.0 t/ha), ‘Sh-11’ (11.9 t/ha), ‘Sh-12’ (13.8 t/ha ha), ‘Sh-13 (13.2 t/ha), and ‘Sh-14’ (13.0 t/ha). Two accessions, which came from the Kyivska (‘Sh-3)’ and Dnipropetrovska (‘Sh-6’) Oblasts were found to be best adapted to the environmental conditions of the Right-Bank Forest-Steppe of Ukraine. Their Lewis’s phenotypic stability factors were close to 1. A local form from the Dnipropetrovska Oblast, ‘Sh 9’ was the worst adapted accessions, as its Lewis’s phenotypic stability factor was as high as 1.2. The maximum leaf length was recorded for accessions from the Kyivska (‘Sh-1’, ‘Sh-2’; 38.0 and 35.8 cm, respectively) and Chernihivska (‘Sh-10’; 36.2 cm) Oblasts. Conclusions. Due to this study, shallot accessions with short growing periods, great weights and numbers of bulbs and yield were selected for the conditions in the Right-Bank Forest-Steppe of Ukraine. ‘Sh-1’ sample originating from the Kyivska Oblast showed the best results in terms of the bulb weight (26.7 g) and yield (30.5 t/ha). However, it turned out to be late-ripening (86 days), and only formed on average 4 bulbs per clump. The maximum leaf length was 38 cm was. ‘Sh-8’, a local form from the Dnipropetrovska Oblast, was the most early-ripening (74 days).

Pengujian Konsorsium Mikroba dalam Upaya Menekan Intensitas Penyakit Hawar Daun (Botrytis squamosa Walker.) pada Tanaman Bawang Merah (Allium ascalonicum L.)

Microbial Consortium Testing to Reduce the Intensity of Leaf Blight (Botrytis squamosa Walker.) on Shallots (Allium ascalonicum L.). Shallots (Allium ascalonicum L.) are a popular horticultural crop that has been grown for centuries. One of the diseases that target the leaves of the shallot plant is Botrytis squamosa Walker. A microbial community is being used to reduce the severity of the fungus Botrytis squamosa Walker's disease. The goal of this study was to see how the microbial consortium affected the severity of leaf blight on shallot plants. The shallot cultivar Batu Ijo was employed in this investigation. This study was carried out at Margaasih Village, Cicalengka District, Bandung Regency, from March to June 2022. The Randomized Block Design (RBD) approach was employed in this investigation, which included 5 treatments and 5 replications. Microbiological consortium package A, microbial consortium package B, microbial consortium package C, microbial consortium package D, and controls were utilized in the treatments. This observation yielded parameters such as attack intensity, plant height, number of tubers, and tuber weight. The results demonstrated that the package B microbial consortium, which included the microorganisms Trichoderma sp. and Gliocladium sp. could suppress the severity of leaf blight (Botrytis squamosa Walker.)

The Potential of Rhizophagus intraradices and Trichoderma asperellum to Induce Shallot Resistance against Twisted Disease

Twisted disease caused by Fusarium spp. is one of the primary diseases on shallots with potency to cause enormous losses by causing stunting and bulb rot. One alternative disease control is the induction of plant resistance since the seed stage. The aim of this study was to determine the content of salicylic acid, jasmonic acid, and phenolic compounds of shallot bulb seeds coated with biological control agents as a resistance response to twisted disease. The shallot cultivar used was Crok kuning. The treatments used in this study were the type of biological control agents, including Rhizophagus intraradices and Trichoderma asperellum, combined with and seed coating application time (one month before planting and simultaneously at planting). Biological control agents in the form of powder formulation applied as seed coating material and seeds were stored for one month before planting. The results showed that application of biological agents delayed the occurrence of the twisted disease symptoms. The salicylic acid content from plant treated with R. intraradices at the time of planting was slightly higher than the control. The jasmonic acid content in T. asperellum plants treated at planting was higher than then control. Total phenolic content from plants treated with T. asperellum at planting time was higher than the control. In general, application of biological control agent as seed coat did not result in significant increase in salicylic acid, jasmonic acid nor the phenolic compounds, compared to the pathogen infected control.

BIO-CATHARANTIN EFFECTS ON PHENOTYPIC TRAITS AND CHROMOSOME NUMBER OF SHALLOTS (ALLIUM CEPA L. VAR. ASCALONICUM 'TAJUK')

The seasonal production of Allium cepa var. ascalonicum causes a rise in its demand during the off-season. Consumers mostly prefer onion cultivars like the 'Super Philip', because of their high productivity, large and round bulbs, shiny appearance, and less spicy taste. In plant breeding, polyploidy induction through mutagens is a technique often used to produce shallot cultivars of better quality. Bio-Catharantin from the leaf extract of Catharanthus roseus L. is used as a polyploid induction agent instead of colchicine. The latest study aimed to determine the effect of Bio-Catharantin concentration (0.2% and 0.4%) on phenotypic traits (plant height, bulb mass, and the number of bulbs), and the chromosome number to determine the minimum concentration that could cause polyploidization in shallots. The research was conducted from December 2020 to February 2021 in a greenhouse in Madurejo, Prambanan, and the Laboratory of Genetics and Breeding, Faculty of Biology, Gadjah Mada University, Indonesia. Bio-Catharantin concentration did not affect plant height which was comparable with the control. Both treatments caused an increase in bulb mass up to 37.7 and 41.76 g at the concentrations of 0.2% and 0.4%, respectively, compared with the control (31.47 g). The number of bulbs increased up to 10.6 and 9.8 g for 0.2% and 0.4% concentrations, respectively, compared with 8.8 in the control. The ploidy level of cells was increased from 2n (16) to 3n (24) at 2% and 4n (32) at 4% Bio-Catharantin.

Karyotype analysis of shallot (Allium cepa L.) cultivars from Southeast Sulawesi for breeding programs and sustainable food availability

This research was motivated by the potential sources of genetic diversity of shallot cultivars, originated from several districts in Southeast Sulawesi. Sources of genetic diversity are one of the defining factors of the success of plant breeding programs to develop new cultivars with the desired traits. The purpose of this study was to identify the genetic characteristics of several shallot cultivars from Southeast Sulawesi through Karyotype Analysis. This research was conducted at the Agrotechnology Laboratory, In Vitro Unit, Agriculture Faculty, Halu Oleo University.This research used methods which were according to Carnoy I in fixating, Gertsel in hydrolysing and La Cour in staining the chromosome. The observed variables in this study were chromosome size, centromere index and chromosome shape. Data analysis was determined based on the results of chromosome length measurements using ImageJ software, Photoshop CS3 and Inkscape. The result showed Wakatobi, Konawe and Buton cultivars had different karyotype formulas, while Bombana and Bau-bau cultivars had similar karyotype formulas.

No Effect of Biostimulants on the Growth, Yield and Nutritional Value of Shallots Grown for Bunch Harvest

Shallots (Allium cepa L. Aggregatum group) are cultivated on small areas, mostly to harvest mature bulbs with dry scales. Due to their exceptional taste and nutritional value, and a short growing season, they can also be grown for early bunch harvest. New shallot cultivation strategies are being sought to meet consumers’ growing expectations regarding the quality of vegetables, and their increasing awareness of global food safety. Therefore, the aim of this study was to evaluate the effect of selected biostimulants on the biometric parameters, yield and nutritional value of shallot bulbs and leaves. The experimental factors were as follows: two biostimulant types—Effective Microorganisms (EM) and Goëmar Goteo (GG), two shallot cultivars—Bonilla F1 and Matador F1, grown for bunch harvest, and year of the study. Shallot leaves had a higher content of L-ascorbic acid, reducing sugars and nitrates than bulbs. Young bulbs had a higher content of DM and total sugars than leaves. The leaves and bulbs of shallot plants treated with EM accumulated the highest amounts of minerals. Macronutrient ratios were closer to optimal in shallot leaves than bulbs. The nitrate content of bulbs was inversely proportional to the nitrate content of leaves. Therefore, an increase in the nitrate content of leaves by around 330% led to an approximately 40% decrease in the nitrate content of bulbs. The correlations between the parameters of the chemical composition of shallots and shallot leaves show that the increase in the dry matter content of the bulbs (by approx. 60%) was accompanied by an increase in the L-ascorbic acid content in the leaves (by approx. 240%). The use of biostimulants in the cultivation of A. cepa L. Aggregatum group contributed to the reduction of L-ascorbic acid content in bulbs and had no positive effect on the leaves. Moreover, no positive effect of biostimulants on the reduction of nitrate content in shallot leaves and bulbs was observed, which is undesirable from the consumer’s point of view. After the use of biopreparations, the yield of shallots was lower than that of the control—by approx. 14% (EM) and approx. 4% (GG). Therefore, the measurable benefits of biostimulants in the cultivation of shallots grown for early bunch harvest do not balance the costs of their purchase and use.

Characterization and expression of the partial flowering locus t-2 gene in the shallot (Allium cepa var. Aggregatum) cultivar ‘Lokananta’

Shallot (Allium cepa var. Aggregatum) is an economically important nutritive vegetable and medicinal plant. Given their low seed production, shallots are vegetatively propagated by using bulb material. Flowering is essential for transferring important traits, such as resistance to Fusarium oxysporum and tolerance to salinity. However, the flowering abilities and times of shallot cultivars are very diverse. Therefore, studying the mechanism and regulatory genes of flowering is mandatory. The AcFT2 gene has a significant correlation with flowering in shallots. The present research aims to obtain information on the gene sequence, relative expression, and correlation of AcFT2 with flowering in shallots under vernalized and nonvernalized conditions. This study was conducted from August 2019 until September 2019 at the Center for Tropical Horticulture Studies, IPB University, Tajur, Bogor, Indonesia. Gene isolation and expression analyses were conducted from October 2019 to July 2020 at the Horticulture Laboratory, Gifu University, Japan. The shallot cultivar ‘Lokananta’ was subjected to vernalization at 8 °C for 6 weeks under nonvernalization treatment and then planted for 30 days for gene isolation. The AcFT2 gene sequence generated from the shallot cultivar ‘Lokananta’ was analyzed by using Geneious, MUSCLE, and Molecular Evolutionary Genetics Analysis. Gene expression was analyzed via qRT-PCR. Results showed that the AcFT2-like gene obtained from shallot had high homology with other FT genes from other plants, especially plants in the Allium genus. The shallot cultivar ‘Lokananta’ showed relatively similar expression as the partial shallot AcFT2-like gene under vernalization and nonvernalization treatments given that the number of umbel flowers did not significantly differ between both treatments.

Preference, population development, and molecular characteristics of Spodoptera exigua (Lepidoptera: Noctuidae) on shallot cultivars: A field trial scale

Abstract. Supartha IW, Susila IW, Sumiartha IK, Rauf A, Cruz LBDC, Yudha IKW, Utama IWEK, Wiradana PA. 2021. Preference, population development, and molecular characteristics of Spodoptera exigua (Lepidoptera: Noctuidae) on shallot cultivars: A field trial scale. Biodiversitas 23: 783-792. The beet armyworm, Spodoptera exigua Hubner (Lepidoptera: Noctuidae), is a polyphagous insect that is the main pest and is responsible for the decline in shallot production in Indonesia. This study aims to (i) analyze preferences; (ii) colonization time; (iii) population development; (iv) attack of armyworms according to plant phenology in the shallot cultivars; and (v) identification species of armyworms. The research was conducted in Bangli Village, Tabanan Regency, Bali Province, Indonesia. The research design used was a randomized block design with the treatment of five shallot cultivars and five replications. Molecular analysis of armyworm larvae was carried out using the Polymerase Chain Reaction (PCR) method using forward Lep_F1 and reverse Lep_R1 primers at the Cytochrome Oxidase Subunit I (COI) gene. Our results indicate that the period of adult pest infestation has been seen since the emergence of plants, namely 7 days after planting (dap). The colonization period begins at 21 (dap), when plants and larvae begin to grow. The development of the egg and larva population was seen when the plants totaled 21 (dap) (for eggs) and 28 dap (for larvae). The most preferred shallot cultivars are "Bali Karet" and "Bima Brebes". Armyworm larvae, including S. exigua, were identified as related to the same group as isolate larvae from China, India, Pakistan, Thailand, and Japan. These findings indicated that S. exigua preferred the two cultivars of shallots in the fields and emerged at 21 dap. This shows that insect management activities must be carried out systematically both when the shallots plants are young, pre- and post-harvest. Control with natural enemies can also be done for further research.

Embryo-gynogenic induction of Indonesia shallot (Allium cepa L. Aggregatum group) cultivars using three different protocol unpollinated flower cultures

Embryo-gynogenic induction was an important step in haploid induction. The research was determined the best method of unpollinated flower culture of Indonesia shallot varieties. Three methods were used including method 1 with two-step cultures using BDS as basic medium supplemented with polyamines 2 mM putrescine on the first step and spermidine 0,1 mM at the second step, method 2 using BDS as basic medium supplemented with 2,4-D 2 mgL−1 and BA 2 mgL−1 on the first step and NAA 1 mgL−1 and 2iP 2 mgL−1 on the second step and method 3 using B5 medium supplemented with 2,4-D 2 mgL−1 and BA 2 mgL−1. Six shallot cultivars used as flower donor were Katumi, Bima Brebes, Tajuk, Trisula, Superphilip, and Bauji. Flower umbel was collected and pretreated using Yoshida liquid medium then placed on incubator at four °C for overnight. The results showed that all flowers cultured could anthesis properly in the first week of cultured. The embryos could emerge from ovaries from week 7th of cultured in all methods. However, the highest percentage of embryos gynogenic developed to seedlings was achieved using method 3 in Superphilip (6.98%) followed by method 2 in Tajuk (4.44%).

Improving seed germination and seedling growth of true seed shallot (TSS) using plant growth regulator seed priming

Seeding is the most important and quite challenging stage in seed/TSS cultivation. Plant growth regulator (PGR) seed priming has the potential to enhance the quality of TSS seedlings. The research had been conducted in the greenhouse of Agriculture Faculty, UGM, from February to April 2020. The factorial treatments of shallot cultivars (Tuk-Tuk, Lokananta and Sanren) and seed treatments (soaking seed with GA3 100 ppm and NAA 50 ppm for 12 hours and untreated seeds as control) were assigned in the RCBD with four replications. Each experimental unit was consisted of 728 seeds per cultivar. Both treated and untreated seeds were sown in soil blocks. The data were analyzed using ANOVA and continued with Tukey HSD procedure at α=0.05. The results showed that PGR priming on seeds significantly increased the germination percentage, plant height, leaf number, leaf area, and hypothetical vigor index of the three cultivars at six weeks after sowing. However, there was no significant difference in the fresh weight and dry weight of seedlings. This research implied that soaking seeds in GA3 100 ppm and NAA 50 ppm for 12 hours could improve seed germination and TSS seedling growth. Additional fertilizers are perhaps needed in soil blocks to increase the effect of hormones on the seedlings.

Variability and relationship of six Indonesian shallots (Allium cepa var. ascalonicum) cultivars based on amino acid profiles and fried shallot’s sensory characteristics

Abstract. Yofananda O, Sobir, Wijaya CH, Lioe HN. 2021. Variability and relationship of six Indonesian shallots (Allium cepa var. ascalonicum) cultivars based on amino acid profiles and fried shallot’s sensory characteristics. Biodiversitas 22: 3327-3332. Shallot is an essential spice in the daily menu and processed food due to its unique flavor. Therefore information regarding the correlated compounds responsible for flavor in several cultivars of shallot will be important to elucidate. Since the amino acids profile is a key component of flavor character, this study aimed to determine the variability of amino acid profiles among six shallot cultivars related to fried shallot’s sensory characteristics. The six shallot cultivars were Bima Brebes, Bauji, Batu Ijo, Tajuk, Super Philip, and Rubaru. The shallot bulb was cultivated in the same area. The bulb size was analyzed using an analytical balance, the amino acids profile was analyzed using HPLC instrument, and sensory characteristics of fried shallots were analyzed using the rate-all-that-apply descriptive method. The result showed that the shallots contained a high content of glutamic acid, arginine, and aspartic acid. Based on fresh weight, Batu Ijo was significantly different than the other cultivars which comprised Tajuk, Rubaru, Bauji, Super Philip and Bima Brebes. Clustering based on amino acid profiles of the fresh bulb, they were divided into three groups. The first group consisted only Bauji, and the second group consisted of Rubaru, Batu Ijo, Super Philip and Tajuk, the third group was only Bima Brebes. Based on the sensory characteristics of fried shallots, they were divided into three groups. The first group comprised of Bauji and Tajuk, the second group comprised of Rubaru alone, and the third group comprised of Batu Ijo, Super Philip, and Bima Brebes. The Pearson correlation analysis showed that L-phenylalanine, L-arginine, and glycine have a significant positive correlation with a sweet taste of fried shallot, indicated that amino acids profile is an important parameter for shallot flavor.

Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance

Abstract. Basundari FRA, Sulistyaningsih E, Murti RH, Nuringtyas TR. 2021. Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance. Biodiversitas 22: 3127-3135. Shallot, an Allium species with high economic value, is widely cultivated in Indonesia. The species includes numerous cultivars with unique characteristics, such as harvesting time, yield, taste, odor, and bulb color. Tuk Tuk and Trisula, the cultivars used in this experiment, have different bulb colors. Tuk Tuk’s bulb color is light red, while that of Trisula is dark red. This color difference may be attributed to variations in the metabolite contents of the shallot bulbs. The present experiment aimed to reveal differences in the metabolite contents of the two shallot cultivars by using Nuclear Magnetic Resonance (NMR) and then determine the specific metabolites contributing to these differences. Bulbs of Tuk Tuk and Trisula were planted in the field under the same conditions and then collected. The bulb samples were powdered, freeze-dried, and subjected to a two-phase extraction method with CDCl3 and D2O as solvents to separate the nonpolar metabolites from polar ones. The extracts were analyzed by 1H-NMR, and the spectra collected were analyzed using MNOVA software and Metaboanalyst.ca. A total of 23 metabolites were successfully identified and characterized in this experiment. The contents of eight of these metabolites, namely, sucrose, glutamine, citric acid, choline, methiin, propiin, threonine, and formic acid, were significantly higher in Trisula than in Tuk Tuk. These differences may be correlated with variations in the color intensity, pungency, and other traits of the cultivars. The results demonstrate that NMR metabolite profiling could effectively differentiate metabolite profile variations among shallot cultivars.

Soil characteristic and shallot growth with gypsum and zeolite amendments in irrigated saline Alfisol and Inceptisol

Salinity of soil and irrigation is a factor that may cause a decrease in shallot productivity, so it requires efforts with amendments. This research aimed to determine the effect of gypsum and zeolite amendments on soil and shallot growth with saline irrigation. A pot experiment was conducted in the field using a completely randomized design with three factors. The first factor was two soil types (Alfisol and Inceptisol); the second factor was three shallot cultivars (Brebes, Purbalingga, and Pemalang); and the third factor was two types of soil amendments. The results showed that gypsum and zeolite reduced pH, sodium adsorption ratio (SAR), electric conductivity paste (ECp) and Na of the soils studied. Gypsum and zeolite increased the uptake of N, P and K by shallot plants. The increase of N uptake by applying gypsum on Inceptisol was more effective to Brebes and Purbalingga cultivars than Pemalang cultivar. Gypsum increased the diameter and number of bulbs in Inceptisol. Zeolite and gypsum increased bulb weight of Purbalingga cultivar in Alfisol.

Application of organic fertilizer and Pseudomonas fluorescens on the growth and yield of shallot cultivar Sabu Raijua (Allium ascalonicum L .) in dry land

The specific objectives of this study were 1 ) knowing certain types of organic fertilizers on the growth of shallots 2 ) knowing the concentrations of Pseudomonas fluorescenss certain the growth of shallots, 3 ) knowing the types of organic fertilizers and the concentrations Pseudomonas fluorescens specificity increase the optimal yield of shallots. To achieve this goal, this research was conducted using factorial experiments with a split Plot Design with 10 treatments and 3 replications. So that there are 10 treatment combinations of a total number of 30 experimental plots. There were 2 factors that were tried, namely the first factor of Organic Fertilizer as the main plot, namely: cow manure 10 ton ha-1 (K1), chicken manure 10 ton ha-1 (K2). While the second factor as the subplot is the concentration of Pseudomonas fluorescens: Watering with water (as a control) 100 ml (P0), Watering with a concentration of Pseudomonas fluorescens 5 ml + normal water 95 ml (P1), Watering with a concentration of Pseudomonas fluorescens 10 + ordinary water 90 ml (P2), sprinkling with a concentration of Pseudomonas fluorescens 15 ml + 85 ml plain water (P3), Flushing with a concentration of Pseudomonas fluorescens 20 + ordinary water 80 ml (P4). The shallot cultivar of Sabu Raijua which was given organic fertilizer of 10 tonnes of chicken manure. Ha-1 and a concentration of Pseudomonas fluorescens 20 + 80 ml of plain water gave the highest growth component at the age of 10 WAP, namely at the age of 10 WAP, namely plant height (37.667cm). Leaves (34, 800 trees), number of tillers (10, 533 trees). The results of shallot bulbs of Sabu Raijua cultivar from organic fertilizer treatment of 10 ton ha-chicken manure1s with a concentration of Pseudomonas fluorescens 20 ml + 80 ml water resulted in components, namely tuber weight per plot (276.70 g ), number of tubers per plot (291, 70 tubers ).

Application of organic fertilizer and Pseudomonas fluorescens on the growth and yield of shallot cultivar Sabu Raijua (Allium ascalonicum L .) in dry land

Application of organic fertilizer and Pseudomonas fluorescens on the growth and yield of shallot cultivar Sabu Raijua (Allium ascalonicum L .) in dry land

The Effects of Biochar and Compost on Different Cultivars of Shallots (Allium ascalonicum L.) Growth and Nutrient Uptake in Sandy Soil Under Saline Water

High salinity irrigation will cause nutrient uptake and shallot growth to deplete due to its sensitivity to salinity. This research aimed to investigate the effect of biochar and compost on shallot growth and nutrient uptake in sandy soil like Entisol when irrigated using salty water. This research carried out some pot experiments and put on the field using a completely randomized design. The first factor was based on the amendment with control of 20 tons ha<sup>-1</sup> of Biochar, 10 tons ha<sup>-1</sup> of compost, and a mixture of 10 tons ha<sup>-1</sup> biochar and 5 tons ha<sup>-1</sup> of compost; the second factor was composed of 3 shallot cultivars (Brebes, Pemalang, and Purbalingga). Each pot was filled with 15 kg of soil; the pot 30 cm in diameter, incubated with saline water and irrigated by 2 dS m<sup>-1</sup>. The results show that irrigation with ground saline water causes Entisol to increase exchangable Na, Ece and SAR, decrease exchangeable Ca and Mg. The application of all treatments decreased SAR. The application of compost significantly increased N and K uptake but made soil pH, EC, and SAR to decrease while fresh weight and dry weight of bulb in Brebes cultivars increased. The combination of biochar with 5 tons ha<sup>-1</sup> of compost produced the highest yield on plant height and number of tuber in Pemalang cultivars and had the largest tuber diameter in Purbalingga cultivars. There is an interaction between amendment and cultivar on plant height, fresh weight of tuber (P<0.01), dry weight of tuber (P<0.01).