The paper reports on the use of a developed novel and specific test-rig for the assessment of performance of radial lip seals of automotive transmissions, with particular emphasis on determination of thermal performance. The test-rig can accommodate original components from a donor vehicle, thus accurately represents vehicular conditions. The specific aim is to experimentally determine the effect of shaft surface roughness parameters on the generated contact temperature. Excessive exposure to high temperatures can accelerate degradation and aging of elastomeric seals, resulting in increased wear. Corundum and CBN (Cubic Boron Nitride) ground shafts are used to ascertain the effect of surface roughness parameters in the shaft seal conjunction. An analytical thermal model is also developed in combination with temperature measurements to determine the exact temperature in the lip seal-shaft contact. It is shown that depending on the type of shaft surface grinding process some potential correlation exists between roughness parameters and generated contact temperatures. It is also shown that the contact temperature can be several degrees Celsius higher than those measured in the proximity of the seal contact.