Urial Research Articles

Modeling the Distribution and Environmental Preferences of the Ladakh Urial in the Arid Himalayas.

Mountains play a crucial role in shaping the climate of an area and subsequently, the environments and species that are suited to those particular conditions. Understanding the relationships between environmental conditions and their influence on the occurrence of a species is necessary to make informed decisions when conserving relevant habitats. This study evaluated and compared the potential for different interactions between climatic variables to describe the environmental preferences and the range of the Ladakh urial, a narrowly distributed sub-species of Ovis vignei, in the arid Himalayas. Species distribution models were created from spatially thinned presence-only occurrence data of 50 locations using a Maximum Entropy (Maxent) model. For this, we subdivided the modeled distribution into environmental categories with varying degrees of suitability. We identified a high cold precipitation to annual precipitation ratio typical of cold desert climates well suited to describe the highest quality environments for urial. The inverse, low warm precipitation to annual precipitation ratio was found to better describe the urial's range at the lower end of potential habitat. In this comparison, we observed that with the exclusion of unsuitable cold steppe habitat with a higher warm precipitation to annual precipitation ratio, we were able to refine the extent of this species range but at a trade-off to the accurate description of high-quality environments. This study demonstrated the strong potential of cold arid climatic conditions to describe the distribution of urial in India, which have implications for identifying important areas for the conservation and management of the species in the high altitude rangelands of Ladakh.

<b> </b>SEASONAL<b> </b>VARIATION AND PREVALENCE OF GASTROINTESTINAL PARASITES IN CAPTIVE MAMMALS AT LAHORE ZOO

This study was conducted at Lahore Zoo, Punjab, Pakistan from January to December 2023 to assess the prevalence of gastrointestinal parasites in 10 different captive wild mammalian species. The mammals including girrafe (Giraffa), vervet monkey (Chlorocebus pygerythrus), zebra (Equus quagga), lion (Panthera leo), leopard (Panthera pardus), Rhinoceros (Rhinocerotidae), wild boar (Sus scrofa), Chinkara deer (Gazella bennettii), urial (Ovis vignei) and nilgai (Boselaphus tragocamelus) were selected. A total of 100 fresh fecal samples were collected from the animal enclosures in polythene bags (6×4 inches) 2 times during study period. Coprological examination was done using qualitative analysis such as direct smear method, sedimentation method and flotation methods. The parasitic prevalence was as follows zebra 44.5%, followed by giraffes, rhinoceroses, and wild boars at 27.8%, nilgai, urial, lion and vervet monkey at 22.3%, chinkara 16.7% and leopard 11.2%. The prevalence of gastrointestinal parasites was as Eimeria spp. (3.9%), Strongyloides spp. (3.4%), Trichuris spp. (2.8%), Giardia spp., Ascaris spp., and Strongyle spp. (2.3%), Entamoeba histolytica (1.12%), Trematode eggs (1.12%), Nematodirus (1.12%), Balantidium coli (0.6%), Haemonchus contortus (0.6%), Paramphistomum cervi (0.6%), Parascaris equorum (1.12%) and Cryptosporidium (1.12%). The overall parasitic prevalence was 23.9%, with a noticeable seasonal variation, being higher in summer (31.2%) compared to winter (17.79%). These findings highlight the significant parasitic burden in captive wild mammals. It can be concluded that regular de-worming sessions, cleaning, disinfection of enclosures and proper disposal of fecal matter should be done to reduce the overall parasitic burden.

Ungulates conservation in the face of human development: Mining and roads' influences on habitat and connectivity in Iran's central plateau

Anthropogenic activities such as mining and road construction pose significant threats to ungulate species in the central Iranian plateau. We conducted a comprehensive study focusing on three key ungulates: wild goat (Capra aegagrus), urial (Ovis vignei), and jebeer gazelle (Gazella bennetti). These species rely heavily on migration corridors for functional connectivity, making them vulnerable to the habitat fragmentation caused by human development. Our research integrates ensemble habitat suitability modelling and connectivity analysis to assess the impacts of mining and road construction on the core habitats of these ungulates and their habitat connectivity. Ensemble models predicted large suitable habitats for all three species; however, a considerable portion of these habitats was compromised by the development of mining roads, highlighting the urgency of conservation actions. We found that wild goat habitats were patchy and fragmented, whereas urial and jebeer habitats were more expansive and better connected. Analysis of core habitats identified critical areas for the species survival. For instance, an important core area for wild goats was located in the central part of the landscape, whereas the core habitat for urials was situated in the eastern part. Multiple core areas were identified for jebeer gazelles, highlighting the diverse habitat preferences of the species. We found extensive overlap between the core habitats of the three species and conservation areas (approximately 80%). Connectivity simulations revealed strong conservation network coverage (between 81 and 91%), emphasizing the importance of conservation areas. Our findings underscore the immediate need for conservation interventions to mitigate the impacts of road development on ungulate habitats and migration corridors in central Iran. This research contributes vital information for informed decision-making in conservation planning and sustainable development in the region.

Safeguarding the last stronghold: Ecology and conservation of Asiatic Cheetah's prey species in Turan Biosphere Reserve (Iran)

Identifying suitable habitats for wild felids is essential for effective area-based conservation efforts. However, modeling habitat suitability for extremely rare and endangered taxa such as the Asiatic Cheetah (Acinonyx jubatus venaticus) is challenging due to limited data availability. To address this, our study focused on analysing the distribution factors of the Asiatic Cheetah's main prey species, including the Wild Goat (Capra aegagrus), Urial (Ovis vignei), Goitered Gazelle (Gazella subgutturosa), Jebeer Gazelle (Gazella bennettii), and Cape Hare (Lepus capensis) in the Turan Biosphere Reserve (TBR), a critical Cheetah habitat in Iran. To determine habitat suitability for these prey species, we conducted a systematic field survey along 30 transects measuring 16 km each by collecting data in each 1 km interval, resulting in 1172 direct observations of their presence. These observations were then correlated with 44 variables grouped into four categories: bioclimatic, topography, vegetation, and distance to anthropogenic and natural features. Suitability maps were generated using a hierarchical modeling approach. Among the observed prey species, Urial had the highest frequency of sightings (675 visual observations over eight seasons), followed by Goitered Gazelle (564 observations), Cape Hare (278 observations), Jebeer Gazelle (232 observations), and Wild Goat (208 observations). The most influential predictors affecting prey species distribution were mean summer temperature (with optimal ranges at lower temperatures), terrain relief roughness (with optimal ranges at intermediate roughness), distance to wells (with optimal ranges at shorter distances), and distance to corrals (with variable optimal ranges across species). Our findings indicate that the western and eastern mountainous areas, along with their hilly surroundings, as well as the northwest and western plains of TBR, constitute the most suitable habitats for these five prey species. These models and suitability maps provide crucial evidence for implementing conservation measures in the vulnerable habitats of the Asiatic Cheetah and its primary prey species. Environmental conditions can be enhanced through the management of water sources, roads, corrals, and livestock grazing. In addition, the fragmentation of suitable areas within TBR highlights the importance of preserving corridors between these fragmented habitats. We recommend re-evaluating and expanding the boundaries of Turan National Park, Turan Wildlife Refuge, and Turan Biosphere Reserve to ensure the long-term conservation of this threatened felid species and its associated ecosystems.

Gastrointestinal parasites of the wild ungulates (Mammalia: Cetartiodactyla) in the Hemis National Park, Ladakh, India.

We evaluated the gastrointestinal parasitic prevalence and intensity of wild ungulates in the Hemis National Park of Ladakh by examining their fresh faecal samples. A total of 327 fresh faecal samples from three wild ungulates, blue sheep Pseudois nayaur (n = 127), Ladakh urial Ovis vignei vignei (n = 110), and Himalayan ibex Ibex siberica (n = 90) were collected between June 2021 and May 2022. The techniques of flotation and sedimentation were used to find parasite eggs and oocysts from the faecal samples. Out of 327 samples examined, 165 samples were infected with gastrointestinal parasites making an overall prevalence of 50.45%. Seven parasitic taxa, including one protozoan (Eimeria spp.), five nematodes (Nematodirus spp., Strongyloides spp., Haemonchus sp., Trichuris sp., and Trichostrongylus spp.), and one cestode (Monezia spp.), were found during the current investigation. Mixed infection was reported in 36 (11%) of the total examined samples. The prevalence of gastrointestinal parasites was found to be highest in blue sheep (55.11%), followed by Ladakh urial (49.09%) and Himalayan ibex (45.55%). The highest prevalence was recorded in the summer (64.42%), and the lowest in the winter (33.82%). A significant difference in parasitic prevalence was observed across seasons in each of the three wild ungulate hosts. However, there was no significant difference in the prevalence of parasites between these hosts.

Demographic history of the Punjab urial and implications for its management

AbstractThe Punjab urial (Ovis vignei punjabiensis) is endemic to Northern Punjab, Pakistan, and is categorized as vulnerable by the International Union for Conservation of Nature Red List of Threatened Species. The urial population has declined by 30% over the last 3 generations. We used non‐invasive fecal samples to identify individuals and estimate population size of Punjab urial in the Kalabagh Game Reserve, Pakistan. We genotyped samples using 12 microsatellite markers to assess genetic variation, population structure, and demographic changes. Microsatellite analysis revealed high levels of genetic variation in urials in terms of expected and observed heterozygosity and allelic diversity. The population structure of the Punjab urial in the Kalabagh Game Reserve, based solely on microsatellite variation using Bayesian clustering, indicated 3 different clusters in the reserve. Results revealed that the urial population may be facing inbreeding pressure because its ancestral effective population size has declined from between 20,000 and 50,000 to ≤1,000 animals today. This reduction has partly occurred because of a bottleneck that occurred about 10,000 years ago. Results also indicate that 1 urial population cluster has the signature of a bottleneck, which may be due to population isolation. The 3 urial clusters are small and broadly dispersed in a large territory, meaning they could be extirpated without any opportunity for natural re‐population through dispersion. The results of our study support a management strategy that encourages maintaining connectivity between urial localities within the Kalabagh Game Reserve, increased diversity so the effective population size may recover from the historical decline, and the use of data generated here as a baseline of urial genetic diversity in the reserve for monitoring diversity over the long term.

Animal behavior informed by history: Was the Asiatic cheetah an obligate gazelle hunter?

Understanding key ecological adaptations, such as foraging, when a predator is almost extinct is complex. Nonetheless, that information is vital for the recovery of the persisting individuals. Therefore, reviewing historical, ethnobiological and recent records can assist in exploring the species behavioral ecology. We applied this approach to Asiatic cheetahs (Acinonyx jubatus venaticus), which once roamed most west and central Asian countries but now is confined to a few dozens in Iran, at historical (pre-1970) and recent (post-1970) scales. We addressed a widely popular perception that Asiatic cheetahs were subjected to prey shifts from gazelles (Gazella spp.) in open plains areas to urial (Ovis vignei) in mountains because of gazelle populations declines due to anthropogenic influences. We also quantified recent prey choice of Asiatic cheetahs and their behavioral plasticity in foraging different prey species types. Although ethnobiological and historical records suggested that gazelle species were the main prey for cheetahs across their Asian range. However, urial were also commonly reported to be hunted by cheetahs across their historical Asian range, showing that the predation on mountain ungulates is not an emerging hunting behavior in Asiatic cheetahs. We found spatiotemporal plasticity in recent hunting behavior of cheetahs with selective predation on adult urial males. There was temporal overlap in hunting times for plains dwelling versus mountain ungulates, albeit with some minor differences with morning mostly for gazelles while the predation on mountain ungulates was predominantly post-midday. We provided three management implications for the recovery and restoration of cheetahs in Asia. Our work highlighted the importance of historical studies in informing the behavioral ecology of rare species.

Using double-observer surveys to monitor urial and ibex populations in the Hindu Kush of Wakhan National Park, Afghanistan

AbstractWe surveyed the urial Ovis vignei and Siberian ibex Capra sibirica in the Hindu Kush mountain range of Wakhan National Park in north-eastern Afghanistan to determine their population status and identify potential drivers of population change. We conducted two double-observer ground surveys, in April–May 2015 and 2018, in 10 areas (total = 288 km2). Urial herds were mostly composed of both sexes (78% of observed herds), the mean adult sex ratio (females:males) was 100:70, and the mean female:juvenile ratio was 100:53. In 2018 we calculated a urial density of 35/100 km2, compared to 72/100 km2 in 2015. Ibex herds were mostly (79%) composed of both sexes, the mean adult sex ratio (females:males) was 100:103, and the mean female:juvenile ratio was 100:58. Ibex density estimates were similar in 2015 and 2018 (c. 250/100 km2). We discuss the usefulness of the double-observer methods for ungulate surveys, highlight the value of viewshed calculations and discuss the possible causes of urial population decline. To ensure the conservation of these ungulate populations, we recommend continued regular monitoring, measures to address poaching and research to clarify the taxonomical status of urials in Wakhan.

Monitoring post-release behavioural activity of captive-bred urial (Ovis vignei punjabiensis) at Togh Managara Safari Park Khyber Pakhtunkhwa, Pakistan.

The Punjab urial (Ovis vignei punjabiensis) is a globally threatened wild sheep species. In Pakistan the species is legally protected and bred in captivity to release into natural habitats to reinforce wild populations. Vigilance and feeding are critical to survival, though the degree to which captive-bred animals exhibit these behaviours post-release has rarely been investigated. We monitored the daily behaviours of five adult urial after release and classifying behaviours from 0600 hrs to 1800 hrs, replicating each one-hour period twice and recording four observations of each animal per hour. At the herd level, vigilance and feeding behaviours were exhibited equally. Males and females invested equal time in feeding, males were more vigilant (p = 0.001) and more aggressive (p = 0.010), and females were more restful (p = 0.019) or engaged in grooming (p = 0.044) or locomotion (p = 0.002). Females spent more time feeding than being vigilant (p = 0.002). Males maintained high levels of vigilance throughout the day. Patterns of resting 1300 hrs to 1500 hrs and feeding in early morning and late afternoon were common for both sexes. Behaviours classified as 'other' were exhibited equally between sexes. Our results reveal positive indications of captive-bred urial balancing threat detection and energy acquisition post-release and exhibiting natural behaviours and activity patterns. We encourage assessment of survivorship to evaluate long-term effectiveness of captive breeding and release as a candidate restoration programme.

A security game approach for strategic conservation against poaching considering food web complexities

Mitigating the poaching pressure on food webs under multiple constraints (including financial and ecological ones) remains an open problem within conservation. Within this field, mathematically modeling the effects of poaching threats on managerial decision-making is a novel approach. To fill this scientific gap, the present paper uses a security game approach to model the interactions between an environmental manager (defender) and a group of profit-seeking pursuit poachers (attackers) who target species which are nodes of the food web. Based upon the non-cooperative Stackelberg game, the objective of the defender (as leader) is to keep the food web at or near equilibrium through optimally manipulating the populations of an optimal subset of species. In contrast, each attacker strives to maximize monetary profit by hunting an optimal population size of the selected species. The model is validated by a numerical example examining the food web of the endangered Persian leopard (Panthera pardus saxicolor), which lives in Golestan National Park (GNP), Iran. The model provides an overarching biotic intervention strategy to keep the (1 predator-4 prey) food web near equilibrium, while only 2 prey species (the urial (Ovis vignei) and the red deer (Cervus elaphus)) are directly threatened by poachers. The examination revealed that both species population data and poaching data should be taken into account to set effective multi-species conservation prioritization levels. In a sensitivity analysis approach, it was found that, despite the fact that red deer is endangered and preferred by poachers, the deterrent penalty measure should be 1.5 times greater for poaching urial than red deer. The output analysis illustrated that, in order to bring the urial deterrent penalty measure closer to the red deer one, enforcement measures should be about 2.5 times stricter for poaching urial than red deer. The results specifically yield insight into how to optimally conserve a food web equilibrium under poaching pressure and within several constraints.

Assessment of landscape connectivity indices and habitat quality to identify essential habitat patches for Ovis orientalis × Ovis vignei (a case study: Varjin Protected Area, Tehran)

سابقه و هدف: ارتباطات سیمای سرزمین نقش بسیار مهمی در مدیریت منطقه های حفاظت شده دارد. در منطقه حفاظت شده ورجین گونه غالب قوچ و میش البرز مرکزی است که جمعیت آن در 20 سال گذشته به دلیل تخریب زیستگاه و تکه تکه شدن در حال کاهش بوده است. تاکنون مطالعات بسیاری در رابطه با ارزیابی اثرهای محیط زیستی توسعه نقاط شهری اطراف منطقه حفاظت شده ورجین صورت گرفته است، اما هیچ مطالعه ای در رابطه با وضعیت ارتباطات و کیفیت زیستگاه در این منطقه به صورت همزمان انجام نشده است. با توجه به موقعیت قرارگیری این منطقه حفاظت شده در البرز مرکزی، یکی از راهکارهای بسیار مهم در حفاظت زیستگاه های موردنظر، بررسی ارتباطات بین لکه های زیستگاهی و شناسایی کریدورهای جابجایی گونه است. هدف از پژوهش حاضر، تحلیل مسیرهای ارتباطی بین لکه های زیستگاه قوچ و میش در منطقه حفاظت شده ورجین و در نظرگیری کیفیت زیستگاه به عنوان یکی از عامل های تأثیرگذار بر حضور بیشتر گونه در لکه های منتخب است. با استفاده از این روش می توان لکه های زیستگاهی در منطقه را در راستای حفاظت بیشتر اولویت بندی نمود.مواد و روش ها: تصویرهای ماهواره ای لندست هفت و هشت مربوط به سال 1381 و 1397 به عنوان دو نقشه پایه و زمان حال برای استفاده در مدل کیفیت زیستگاه و تحلیل ارتباطات در این پژوهش انتخاب شد. برای بررسی سنجه ارتباطات زیستگاهی در دو مقیاس لندسکیپ و لکه نیز سنجه های dBC و dPC محاسبه شد. نرم افزار مورداستفاده برای این ارزیابی، نرم افزار Graphab بوده که با استفاده از نظریه گراف به صورت شبکه ای از گره ها و مسیرها، ارتباط بین لکه های زیستگاهی را با درنظرگیری آستانه وارد شده و برمبنای روش اقلیدسی محاسبه می کند. از سوی دیگر کیفیت زیستگاه با تأثیرگذاری عامل های انسانی روی کدهای زیستگاهی در دو بازه زمانی به عنوان خدمت اکوسیستمی منطقه حفاظت شده با استفاده از نرم افزار InVEST به دست آمد. درنهایت خروجی هردو نرم افزار وارد محیط ArcGIS 10.4 شده و با طبقه بندی نرمال، طبقات ارتباطات زیستگاهی و کیفیت زیستگاه به دست آمد.نتایج و بحث: نتایج نشانگر این امر است که سنجه dPC در بازهای بین 0 تا 796 / 0 و سنجه dBC در بازه 0 تا 11 + E 58 / 7 به دست آمده است. این مقادیر در محیط ArcGIS ، به 5 گروه متفاوت اهمیت تقسیم شدند و 23 لکه زیستگاهی هرکدام درجه متفاوتی از اهمیت را به خود اختصاص دادند. دو لکه یک و چهار در سمت راست منطقه حفاظت شده اهمیت بسیار زیادی در حفظ دو سنجه dBC و dPC دارند . همچنین خروجی مدل InVEST نشان می دهد کیفیت زیستگاه در نواحی شمال و شمال شرقی منطقه در طبقات کم و در جنوب منطقه نیز در طبقه بسیار کم قرارگرفته است. با اولویت بندی دو نقشه ارتباطات و کیفیت زیستگاه، لکه های زیستگاهی دارای اولویت حفاظت به دست آمد که لکه های سمت شرقی و جنوبی منطقه را تشکیل می دهند.نتیجه گیری: لکه هایی که ازلحاظ ارتباطات اهمیت ویژه ای داشته و همچنین دارای کیفیت زیستگاه بهتری نسبت به سایر لکه های دیگر است، در قسمت شرق و جنوب شرقی منطقه حفاظت شده ورجین قرار گرفت. این مطالعه با قدمی نوین و با استفاده از سنجه های ارتباطی در دو مقیاس لندسکیپ و لکه و با در نظرگیری خدمت اکوسیستمیِ کیفیت زیستگاه به شناسایی لکه های دارای اهمیت در طرح حفاظتی پرداخته و می تواند برای دیگرگونه های سنجه در مناطق حفاظت شده مورداستفاده قرارگیرد. به طورقطع با تغییر مقیاس مطالعه و در نظرگیری دو منطقه حفاظت شده لار و البرز مرکزی در دو سوی ورجین، نتایج مطالعات ارتباطات و درنهایت اولویت بندی تغییر خواهد کرد.

Hostile Interactions of Punjab Urial (Ovis vignei punjabiensis) towards Indian Gazelle (Gazella bennettii) during Feeding Sessions in Captive Breeding Settings.

Simple SummaryMultiple factors, both natural and anthropogenic, are driving most of the wild species to the verge of extinction across the globe. In order to conserve these threatened species, various conservation interventions and strategies are adopted, among which is re-introduction of captive stocks of species into the wild habitats where they vanished from. Captive breeding is one of the promising tools for endangered species preservation. Providing social enrichment to the captive stocks is an important step in the management, in particular for stocks, which are aimed for re-introduction. The subject species of this study, i.e., Punjab urial (Ovis vignei punjabiensis) and Indian gazelle (Gazella bennettii) are being reared in captivity with the aim of re-introduction. As there is scarcity of information regarding the behavioral aspects of captive species, especially ungulates, this study aimed at understanding the dynamics of their interactions. The current study reported that Punjab urial, being the dominant species, exerts itself on the submissive and subordinate species, the Indian gazelle. This negative interference can possibly lead to negative ramifications in the form of stress and injuries in the short term, while negative effects on population growth in the long term. Thus, this study recommends separate rearing of these species to eliminate the hazardous competition between them.Natural wildlife habitats are regularly subjected to anthropogenic pressures for different purposes, which are heading the biodiversity towards drastic decline. Several endangered wild species are raised in captivity with the aim of re-introduction. In some instances, mixed herds’ rearing approach in captivity is adopted for providing social enrichment to captive stocks; however, the impacts of species on each other are least documented. We tested our prediction that keeping mixed herds of captive wild sheep and antelopes provides adequate social enrichment to the captive stocks: if interspecific interactions are balanced. In the current study, we studied the interspecific competition between mixed herds of captive Punjab urial (Ovis vignei punjabiensis) and Indian gazelle (Gazella bennettii) at Manglot Wildlife Park, Nowshera District, Khyber Pakhtunkhwa Province, Pakistan. We documented the negative effects of behavioural interference by Punjab urial on the feeding behaviour of Indian gazelle. The outcome of the current study revealed that Punjab urial are highly intolerant towards Indian gazelle, with high interference during feeding. Out of the total aggressive events, 77% (N = 1259) of events ended up with win/loss, in which Punjab urial dominated the Indian gazelle 3.5 times. Moreover, lopsided dominance by Punjab urial resulted in increased intraspecific competition among Indian gazelle (p < 0.001). Current study divulged Indian gazelle to be the subordinate species, with less intake of food. Instead of providing social enrichment by heterospecifics, the Punjab urial is negatively affecting the Indian gazelle, therefore, the results of our study discourage the practice of admix captive breeding for wild sheep and antelopes.

Species Distribution Models Unveil Niche Partitioning in Bovid Guilds of Southwestern Asia

Despite the widespread coexistence of three guilds of the bovid family — deer [red deer (Cervus elaphus) and roe deer (Capreolus capreolus)], gazelles [goitered gazelle (Gazella subgutturosa) and chinkara (Gazella bennettii)], and goats [bezoar goat (Capra aegagrus), Armenian mouflon (Ovis gmelini) and urial (Ovis vignei)] — in southwestern Asia, detailed information on their habitat selection and niche partitioning is lacking. We used species distribution models (SDMs) based on MaxEnt to assess the suitability of habitats for these species. The results showed that MaxEnt models with the area under the ROC curve (AUC) > 0.85 had a good predictive value. The highest and lowest habitat overlaps were obtained for red deer/roe deer (35.4%) and goitered gazelle/chinkara (3.6%), respectively. Our results provide information on habitat selection and niche partitioning in the studied bovids which can improve their conservation efforts.

Investigation on Parasites and Some Causes of Mortality in Captive Punjab urial (Ovis vignei punjabiensis), Pakistan.

Background:The present study was conducted in Jul 2019 and Jan 2020 in two wildlife parks of the Nowshera district, Khyber Pakhtunkhwa, Pakistan, where the endangered Punjab urial (Ovis vignei punjabiensis) is successfully bred in captivity. We determined diversity of internal and external parasites that take advantage of the situation of congestion, resulting in massive mortalities of wild animals in captivity.Methods:Internal parasites of living urial were determined by direct wet smear and flotation methods, while dead urial was necropsied for any pertaining observation.Results:All examined fecal samples were found infected with gastrointestinal parasites, which had significant difference in the total abundance in winter and summer. S. papillosus and H.contortus, and a single protozoan, Eimeria spp. were the dominant parasites in fecal samples. Ticks collected from urial enclosures and dead animals were of single species H. anatolicum. Theileria spp. was observed in blood, while hydatid cysts were found in lungs and liver of necropsied urial.Conclusion:The study indicates that internal parasites such as Haemonchus contortus and Strongyloides papillosus, while external parasites as Hyalomma anatolicum ticks played major role in the population decline. Strict veterinary control of infectious diseases, provision of hygienic and supplementary diet, and proper maintenance of urial population are necessary measures for the control of mortalities.

Genetic variability analysis of 26 sheep breeds in the Czech Republic

Genetic variability analysis of 26 sheep breeds in the Czech Republic

Determining Optimal Stock Density of Punjab Urial (Ovis vignei punjabiensis) in Captivity for Breeding, Population Growth and Reintroduction Potential.

The Punjab urial (Ovis vignei punjabiensis) is an endangered wild sheep of Pakistan, raised in captivity with the aim of re-introduction. To date, no information is available about population trends of this species in captivity. The current study was conducted with the aim to evaluate the population trend to better guide captive breeding for improved productivity and conservation value. Annual population data recorded and maintained by the Wildlife Department Khyber Pakhtunkhwa, Pakistan, were used. The data were compiled and analyzed in Microsoft Excel 2010 for determining growth rates and package Growthcurver in R-version 3.5.1 was used to produce a graphical representation of the population trend. The overall average annual population growth rate was rN = 0.22. Results revealed a fast initial growth rate with an average value of rN = 0.4 per year. Birth rates of bN = 0.45 for the first nine years were considerably higher than the death rates dN = 0.22 and the population increased with exponential growth. In the subsequent year, very high mortalities rates (dN = 1.2), likely attributed to the clumping of the population, resulted in the collapse of the population, leaving it in a state of unstable equilibrium. Results support the evaluation of management data to reveal carrying capacity in captive populations, to guide and inform appropriate release of surplus animals into natural habitats.

Parasites of markhor, urial and Chiltan wild goat in Pakistan

Parasites are transferred between domestic and wild animals, when host animals come in contact with each other, particularly while grazing the same pastures, or when using same water bodies for drinking. Chances of parasite transmission and adaptation are high when hosts are genetically related. Afghan urial (Ovis vignei blanfordi), Suleiman markhor (Capra falconeri jerdoni) and Chiltan wild goat (C. aegagrus chialtanensis) are wild kin of domestic sheep and goats, sharing numerous parasitic diseases with each other. The present study was conducted in 2014–2015, to determine parasitic infections of Suleiman markhor and Afghan urial of Torghar Game Reserve, and the endemic wild goat of Chiltan National Park. For comparison, parasites of domestic small ruminants of these areas were also studied. A total of 11 species of helminth and 20 species of protozoa were recorded. Highly prevalent helminth among wild ruminants were Trichuris spp., Nematodirus spp., Protostrongylus rufescens and Moniezia benedeni, while highly prevalent Eimeria were E. arloingi and E. ninakohlyakimovae in caprines and E. ovinoidalis in urial. Chiltan wild goats were also found infected with Entamoeba spp. A short tabulated review of the helminth and protozoan parasites of wild sheep and goats of Pakistan, India, Iran and Turkey has been presented.

Assessment of nematodes in Punjab Urial (Ovis vignei punjabiensis) population in Kalabagh Game Reserve: development of a DNA barcode approach

Punjab urial (Ovis vignei punjabiensis) is a wild sheep of Pakistan, considered a vulnerable species by IUCN. Major threats to urial populations include habitat loss and poaching, causing severe declines in its population. Nematode infections may also compromise urial survival, but little is known about Punjab urial gastrointestinal nematodes. In this study, a novel DNA barcoding approach was developed using ITS-I as a target region, with a primer pair designed to amplify frequently reported nematode species for small ruminants. The novel primer pair was validated in silico and in vitro and subsequently used to determine the presence of nematodes in Punjab urial samples from Kala Bagh Game Reserve, District Mianwali (Pakistan). DNA barcoding revealed a higher prevalence of Haemonchus contortus (73.91%), Trichuris ovis (16.30%) and Trichostrongylus axei (3.26%) in Punjab urial. This study demonstrates that the novel DNA barcoding approach is a robust tool to detect nematode parasites from faecal samples of Punjab urial. This method can be used to detect nematode infections in wild and domestic hosts for surveillance and population conservation.

Complementary endozoochorous seed dispersal by large mammals in the Golestan National Park, Iran

AbstractLarge animals tend to disperse seeds over long distances via ingestion and defecation due to their large home range and capacity to move among different habitats for feeding. The aim of this study was to investigate the potential of endozoochorous seed dispersal by five herbivores: Ovis vignei, Capra aegagrus, Gazella subgutturosa, Cervus elaphus, Capreolus capreolus and two omnivores: Sus Scrofa and Ursus arctos in the Golestan National Park, northeast of Iran, by a greenhouse germination experiment. A total of 3107 seedlings belonging to 154 different plant taxa were germinated from 655 dung samples collected in three different habitats. Plant families that most frequently germinated were Poaceae and Brassicaceae. Urtica dioica was the most abundant germinating seed, accounting for 20% of all the seedlings recorded in our dung samples, whereas the most frequently observed species was Portulaca oleracea, which occurred in 24% of our samples. We showed that 54% of the seeds germinating were dispersed by only one of the mammals studied. Herbs and graminoids were the most frequently dispersed growth forms by the herbivores and the wild boar, whereas brown bears mostly dispersed shrubs. The seedling composition in the dung samples was strongly correlated with the local flora especially for non-selective feeders, like red deer. The differences observed in the number of plants and frequency of different growth forms dispersed among the studied mammals reflect their body size, digestive physiology, and dietary and habitat preferences. Our findings highlight the different and complementary roles of large herbivores and omnivores as long-distance seed dispersal vectors.

Evaluation of the role of the national parks for Persian leopard (Panther pardus saxicolor, Pocock 1927) habitat conservation (case study: Tandooreh National Park, Iran)

Having knowledge of the habitat requirements of top predators is important for their conservation and also for the stability of wildlife communities since they are keystone species of ecosystems. This study aims to predict suitable habitats for the Persian leopard (Panther pardus saxicolor, Pocock 1927) in Tandooreh National Park, Iran. This species is the largest leopard subspecies in the Middle East and is at high risk of extinction. Presence points for the species have been obtained from field data, literature review, atlas data, and IUCN records. Variables related to human disturbance, terrain, land use/land cover, and the leopards’ prey were used to predict the habitat selection of the Persian leopard by employing binary logistic regression. Our model predicted that the presence of Persian leopards is restricted to the southern, north-western, and western areas of the park which are under severe fragmentation threat. Moreover, leopards avoided areas near human development such as roads. We have also found that the availability of prey such as Capra aegagrus, Ovis vignei, and Ovis orientalis is one of the important parameters to predict habitat suitability for leopards. Our model provides a suitable tool for national park managers to identify biodiversity hotspots since leopards are often associated with high biodiversity.