Sex Pheromone Trap Research Articles

Physiological Status of Rice Leaf-Roller Cnaphalocrocis medinalis (Lepidoptera: Crambidae) Adults Trapped by Sex Pheromone and Floral Odor.

The rice leaf-roller Cnaphalocrocis medinalis is an important migratory pest of rice. We conducted a study to determine the physiological status of adults trapped by a sex pheromone and floral odor. In the immigrant group, the number of males trapped by the floral odor was greater than the number caught by sex pheromone trapping. The volume of testes was similar in the above two trapping methods but was smaller than in the sweep net method. The ovary developmental grade, mating rate, and number of matings of females caught in floral odor trap were higher than in those caught in the sweep net. In the local breeding group, the number of males trapped by sex pheromones was greater than the number trapped by the floral odor. The volume of testes was smaller in the floral odor trap compared to the pheromone trap group, with the largest in the sweep net group. The ovarian developmental grade, mating rate, and number of matings of females were significantly higher in the floral odor trap group than in the sweep net group. In the emigrant group, the adult olfactory response to the sex pheromone and floral odor was low. The volume of testes was larger in the sweep net group compared to the moths caught by floral odor trapping. The number of eggs laid by female immigrants trapped by the floral odor and sweep net was similar, while the number in the local breeding group was greater in moths caught with the sweep net in comparison with those caught by the floral odor trap. The difference in egg hatchability between the two trapping methods in both immigrants and local breedings was not significant.

Functional Characterization of Peripheral Neurons and a Receptor Recognizing Sex Pheromones in Hyphantria cunea (Erebidae).

Hyphantria cunea (Lepidoptera: Erebidae) is difficult and costly to control as a quarantine pest found globally. Sex pheromone trapping is an effective measure for its population monitoring and control; however, the peripheral neural mechanism of sex pheromone recognition in H. cunea remains unclear. An electrophysiological analysis showed that both male and female moths of H. cunea responded to four components of sex pheromones and the responses of male moths were stronger than those of the female moths. We identified three types of trichoid sensilla (ST) responsive to sex pheromones using the single sensillum recording technique. Each type was involved in recognizing 9R, 10S-epoxy-1, Z3, Z6-heneicosatriene (1, Z3, Z6-9S, 10R-epoxy-21Hy). Four peripheral neurons involved in the olfactory encoding of sex pheromones were identified. Four candidate pheromone receptor (PR) genes, HcunPR1a, HcunPR1b, HcunPR3, and HcunPR4, were screened by transcriptome sequencing. All of them were highly expressed in the antennae of males, except for HcunPR4, which was highly expressed in the antennae of females. Functional identification showed that HcunPR1a responded to sex pheromone. Other HcunPRs were not functionally identified. In summary, neurons involved in sex pheromone recognition of H. cunea were located in the ST, and HcunPR1a recognized secondary pheromone components 1, Z3, Z6-9S, 10R-epoxy-21Hy. Interestingly, PRs that recognize the main components of the sex pheromone may be located in an unknown branch of the olfactory receptor and merit further study. Our findings provide a better understanding of the peripheral neural coding mechanism of type II sex pheromones, and HcunPR1a may provide a target for the subsequent development of highly effective and specific biopesticides for H. cunea.

Non-target Organisms Captured in the Sex Pheromone Traps of Yellow Stem Borer of Rice

Non-target Organisms Captured in the Sex Pheromone Traps of Yellow Stem Borer of Rice

Assessment of Safe Vegetable Cultivation at Jashore in Bangladesh: Evidence from Country Bean, Cucumber and Pointed Gourd Cultivation

The study was designed to assess the present status and methods of safe vegetable production at farm level in selected areas of Jashore during January-May, 2023. For the study ninety sample farmer were selected by stratified sampling from three sub-district of Jashore. Average farm size was 0.79 ha in study area. Farmer doing different agronomic management such as weeding, irrigation, spraying, de-topping, stalking, earthing up etc. Farmer used different methods simultaneously such as insecticide spray, bio-pesticide, sex pheromone, yellow trap, white trap, hand picking etc. for controlling insect pest. About sixty-four percent farmer sprayed synthetic pesticide and rest of them used bio control method such as bio-pesticide (bio-chamak, eco-mac, bio-envir, bio-cin), sex pheromone trap, yellow trap and hand picking for control insect pest in their fields. About fifty-eight percent farmer used bio-fertilizer along with organic fertilizer (manure, vermin compost) and seventy-eight percent farmer used chemical fertilizer for vegetable cultivation. Total production cost of country bean was Tk.3,36,579 ha-1, cucumber was Tk.3,25,915 ha-1 and pointed gourd was Tk.4,40,106 ha-1. Among the cost item labor cost was highest. Average yield of country bean was 30.60 tha-1, cucumber was 30.08 tha-1 and pointed gourd was 41.60 tha-1. Highest benefit cost ratio was cucumber (2.13) followed by pointed gourd (2.07) and country bean (1.50), that means safe vegetable cultivation also profitable. Farmer awareness, havoc attack of pest and insect were the main problem for safe vegetable cultivation. Market facilities, demand, value chain, proper price for organic vegetable, and farmer motivation can promote and enhance safe vegetable cultivation.

Pheromone traps and climate variations influence populations of Sahlbergella singularis (Hemiptera: Miridae) and associated damage of cocoa in Cameroon.

Knowledge of insect pest ecology and biology is important for maximizing crop protection and reducing crop losses. Currently, we lack an efficient control program for the cocoa mirid Sahlbergella singularis Haglund (Hemiptera: Miridae), the principal insect pest of cocoa in West and Central Africa. A 2-yr study was conducted in 11 plantations across Ayos and Konye, two of the largest cocoa growing areas of Cameroon. We evaluated the effects of mirid sex pheromone and climatic variations on mirid population dynamics and their associated cocoa damage. Sex pheromone traps caught 1.5-fold higher mirids in Ayos than in Konye, with more overall counts in 2015 than in 2016. Cocoa pod counts were also significantly higher in 2015 than in 2016 and were negatively correlated with temperature and relative humidity. In both localities, mirid populations and associated cocoa pod damage were suppressed in plantations where sex pheromone traps were used. Damage incidence was positively correlated with mirid counts, confirming that the cocoa pod is the preferential site for mirid feeding and reproduction. As such, damage incidence could be used as proxy for comparative mirid population level due to the mirid's cryptic habit. Of the recorded weather variables, only relative humidity was correlated (negatively) with damage severity. Our data on the relationships between damage caused by mirids and their population dynamics and sex pheromone trap catches suggest that an effective control strategy using mass trapping could be developed for mirid management in cocoa plantations.

Evaluation on Integrated Method of Fall Army Worm Pest Management in Maize Hybrid in Namakkal District of Tamil Nadu, India

Maize (Zea mays L.) on farm trials were conducted by Krishi Vigyan Kendra, Namakkal to assess the management modules against Fall Army Worm (FAW) in maize crop in terms of plant height, number of cob/ plant, cob length (cm), number of grain row/cob, 100 grain weight (g), grain yield, stalk yield and net return, first incidence of FAW days after sowing (no.), damage percentage, natural enemies occurrence (no./sq.m), number of adult moth trapped in sex pheromone trap per day per hectare during Kharif 2019 in Namakkal district. The treatment T6 followed is summer ploughing, seed treatment with Fortezaduo (Cyantraniliprole +Thiamethoxam) @ 4ml/kg, Border crop with grain sorghum as trap crop (advance sowing) and legume intercrop (cowpea) to promote natural enemies (few rows at interval), Collection and destruction of egg masses, installation of sex pheromone trap @ 10 numbers per hectare, Azadirachtin 10000 ppm @ 1 per cent spray at 10 to 15 days after sowing; spraying of Emmamectin Benzoate 5SG @ 0.4g/ litre on 24 days after sowing and Metarhizium anisophilae(1x107) @ 2 ml/ litre on 35 days after sowing and farmers practice on application of pesticides four to five times in crop period revealed that maize hybrid (NK 6240) recorded highest plant height of (105.7 and 194 cm) on 60 and 90 DAS, yield attributes viz., number of cobs per plant is 1.7; cob length is 14.4 cm; number of grain row/cob is 10.8; number of adult moth trapped in pheromone trap (1.3 number), natural enemies occurrence 3.2 number per m2, and found to be non significant in plant height and growth attributes of Maize followed by Treatment T4 and T5, whereas higher grain yield of 64.25 quintal ha-1 and Stalk yield of 94.8 tonnes ha-1, and first incidence of fall army worm larvae 17 days after sowing, lesser damage of fall army worm percentage (7.2%), were found to be significant as compared to farmers practice. The lesser plant height of 89 cm, 171.3 cm, number of cob/ plant 1.3, cob length 11.4 cm, number of grain row/ con is 8.5, and 100 grain weight is 19.1 gm and grain yield of 56.6 quintal ha-1, stalk yield of 61.5 quintal ha-1 and net return of Rs. 10550 /hand first incidence of FAW days after sowing on 11 days, and damage percentage of 11.4 % was recorded under treatment T7. Treatment T6 plot recorded higher net return of Rs.21,575/ ha, respectively by cultivating maize hybrid (NK 6240) as against the T7 with net return of Rs. 10, 550/- with higher damage of cob, respectively by cultivating Maize hybrid (NK 6240). Farmers were very satisfied with Maize hybrid (NK 6240), as crop find fewer incidences of fall army worm and higher return compared to other practices. Maize hybrid (NK 6240) followed under T6 recommended practices would be a better option for management of Fall Army Worm in kharif season in Namakkal district of Tamil Nadu. The aim of this study was to determine the effect of cultural practices, insecticide application at different plant growth stages between seedling emergence and flowering on yield loss caused by FAW in maize.

Prediction of seasonal population dynamics of Grapholita molesta (Busck) and Adoxophyes orana (Fischer von Röslerstamm) in peach orchards using sex pheromone trap and degree-days and its implications in pest management

The successful management of lepidopteran moths in orchards usually depends on the precise forecast of adult activity. However, the seasonal phenology of moths varies between crop cultivars and years, making it difficult to schedule the control measures. Here, we monitored male flight activity of oriental fruit moth Grapholita molesta and summer fruit tortrix moth Adoxophyes orana by using sex pheromone traps in peach orchards of three different cultivars for three successive years. We developed a logistic multiple-peaks model to fit data and then calculated degree-days (DD) required for male activity and neonate emergency. Results show that G. molesta and A. orana males had 4–5 and 3 flight peaks per year, respectively. The seasonal phenology of G. molesta or A. orana was quite stable with an identical timing of each flight peak between cultivars in a year. The flight activity was usually higher in the second and third peaks for both moths, with a higher cumulative number of G. molesta males captured than that of A. orana. Compared to A. orana, G. molesta emerged early in spring and required lower degree-days to reach the subsequent flight peaks and for neonate emergency. Our results suggest that to decline the possibility of outbreaks of moths during the growing seasons, pheromone traps should be scheduled in April with a cumulative DD between 49.6 and 207.1 for G. molesta and in mid-May–early June with a cumulative DD between 450.4 and 866.7 for A. orana, aiming to trap the newly emerged male adults or disrupting female mating success of overwintered moths in orchards. Based on the thermal requirement for egg hatching (i.e., 79.4 DD for G. molesta and 90.0 DD for A. orana), insecticide treatments would be applied in late-April–early May and late May–early June to reduce the field population density of neonates of G. molesta and A. orana, respectively, to reduce fruit damage in orchards. Furthermore, pheromone traps set up in late July–early August (573.8–1025.2 DD) for G. molesta and in mid-September (1539.7–1788.9 DD) for A. orana may suppress overwintering populations and thus decrease pest infestation in next year.

Field Efficacy Testing of Sex Pheromone Trapping with Alternative Trap Desing-Lure Options for Brinjal Shoot and Fruit Borer in Srilanka

Collaborative field studies were carried out to evaluate the effectiveness of improved pheromone trapping system against brinjal shoot and fruit borer Leucinodes orbonalis Guenee in Sri Lanka. First field study comparing five alternative trap designs in two locations for eight weeks confirmed that moth catch was more in funnel trap with two holders (3.08), with the next being funnel trap with three holders (2.09). The catches in delta trap were intermediate (1.81), while other two funnel traps caught 1.05 to 1.84 moths. The differential efficacy of moth catches may be attributed to trap colour, shape, size and other features enhancing arrivals and/or minimising moth escape. The second field study was on pheromone loadings in lures which showed non -significant differences among 3, 5, 7 and 10 mg loadings. These studies confirmed the superiority of promising trap designs for trapping of the brinjal shoot and fruit borer moth in Sri Lanka. Scope for further research to identify more efficient trap designs and optimum pheromone loadings is indicated.

Bats are sentinels for invasive pest surveillance based on DNA metabarcoding

Loss of crop yield due to non-native invasive pests is a global phenomenon with profound population, biodiversity, ecosystem, and economic impacts. The polyphagous and migratory fall armyworm (FAW) Spodoptera frugiperda (Lepidoptera, Noctuidae) has become a major invasive pest around the globe in the past decade, posing a great threat to global food security. Early warning and timely detection of this pest and its associated agricultural risks are essential for preventing, intervening, controlling, and mitigating invasion threats, which could reduce crop losses and economic damage. However, one key challenge is how to effectively and accurately monitor pest invasion as soon as possible prior to their establishment. Here, we aimed to determine the effectiveness of four different methods for identifying the presence of FAW, namely DNA metabarcoding, field survey, light trap, and sex pheromone trap. We collected fecal samples of six bat species in the FAW colonization area, including Rhinolophus pearsonii, Rhinolophus rex, Ia io, Myotis altarium, Myotis pilosus, and Myotis chinensis. We then utilized bat fecal samples to monitor FAW through DNA metabarcoding during its early establishment stages and compared this approach to three traditional surveillance methods. The results revealed interspecific differences in the occurrence frequency of FAW in different bat species. Furthermore, bat fecal DNA metabarcoding successfully monitored the occurrence of this pest nearly one month in advance compared with traditional approaches, which coincides with the adult migration time of larval counterparts found in the field based on effective accumulated temperature calculations. Our results showed that DNA metabarcoding dietary analysis of generalist insectivorous bats can be an effective, noninvasive, and economically viable tool for invasion pests monitoring to inform management decisions.

Determination of Hourly Distribution of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) Using Sex Pheromone and Ultraviolet Light Traps in Protected Tomato Crops

Tuta absoluta (Meyrick), a leafminer that damages tomato leaves, terminal buds, flowers, and fruits, is a destructive tomato pest and is responsible for 80–100% of tomato yield losses globally. Different insect species have different courtship responses and phototropic flight rhythms. Improving the trapping effects of the sex pheromone and light traps is important for constructing an IPM system for T. absoluta. The present study explored the hourly distribution of T. absoluta adults caught by the sex pheromone (on the ground) and UV light (380 nm) traps in greenhouses over 24 h. The responses of males to sex pheromone (false female) lures were detected at dawn and early morning. The responses lasted for 3 h, from 05:30 (1 h before sunrise) to 08:30 (2 h after sunrise), and 95.8% of the males were caught during this period. The peak of the male responses to the sex pheromone was detected at 07:30 (from 06:30 to 07:30, 1 h after sunrise), and 80.8% of the males were caught during this period. The flight of male (proportion of 54.3%) and female (45.7%) adults toward the UV light traps occurred from 19:30 (time of sunset) to 06:30 (time of sunrise), lasted for 11 h, and exhibited a scotophase rhythm; 97.4% of the adults were caught during this period. The peak of adults flying toward the UV light traps occurred at 21:30 (from 20:30 to 21:30, 2 h after sunset). The rhythms of males’ responses to the sex pheromone and of the adults’ flight toward the UV lights can help to reveal the mechanisms of chemotactic and phototactic responses and may play a significant role in constructing an IPM system for this pest.

Effect of pheromone blend sources and loadings on sugarcane internode borer moth catches in Sri Lanka

The internode borer, Chilo sacchariphagus indicus Kapur is an important moth borer on sugarcane in South Asia including India and Sri Lanka. Collaborative studies to evolve locally optimum sex pheromone trapping system for this pest were undertaken in Sri Lanka during 2019-20. The first study comparing alternative pheromone blend sources showed significant differences in moth catches, with the best source consistently attracting twice or more moths than the others. The next study on pheromone lure loadings showed non-significant differences in moth catches, clarifying the adequacy of the widely adopted loading (3 mg/ septa). While these findings could guide in adopting locally optimum blend source and loading for pheromone trapping of the borer moths, the scope of future R&D to further enhance the impact potential of pheromone-based mass trapping of sugarcane internode borer moths in Sri Lanka is indicated.

Evaluation of Rice Genotypes Resistance to Yellow Stem Borer, Scirpophaga incertulas (Walker) through Sex Pheromone Trap

Yellow stem borer, Scirpophaga incertulas (Walker) is monophagous insect pest causing serious damage to rice growers of plain regions of Nepal. This study was done to evaluate the host preference of yellow stem borer to the various genotypes of rice. The research design employed was Randomized Complete Block Design (RCBD) with seven treatments and three replications and done in kharif season of 2021. Rice varieties selected were Sabitri, Sarju 52, US 312, Radha 4, Sawa Mansuli, Silki and Hardinath 3, which are popularly cultivated in Kailali. Sex pheromone trap (scirpolure) was installed to monitor the preference of yellow stem borer for various rice varieties, especially at the panicle initiation stage of rice. Statistical analysis of four weeks data of adult male moths trapped on different cultivars of rice revealed that the yellow stem borer adult moths were highly attracted to the US 312 and less attracted to Sarju 52 and Silki varieties. This research implied that the adoption of popular rice variety Sarju 52 could safeguard the farmers from the notorious pest i.e. yellow stem borer of rice.

Moth sex pheromones affect interspecific competition among sympatric species and possibly population distribution by modulating pre-mating behavior.

Premating behaviors mediated by pheromones play pivotal roles in animal mating choices. In natural populations of the striped stem borer Chilo suppressalis and the rice leaf roller Cnaphalocrocis medinalis in the rice field habitat, we discovered that Z11-16:Ald, a major component of the C. suppressalis pheromone, modulated the premating behavior of C. medinalis. Z11-16:Ald evoked a strong olfactory response in male antennae and strongly inhibited the sex pheromone trapping of male C. medinalis in the field. The functions of three C. medinalis sex pheromone receptor genes (CmedPR1-3) were verified through heterologous expression in Xenopus oocytes. CmedPR1 responded to Z11-18:OH and Z11-18:Ald, as well as the interspecific pheromone compound Z11-16:Ac of sympatric species; CmedPR2 responded to Z13-18:OH and Z13-18:Ald, as well as the sex pheromone compounds Z11-16:Ald and Z9-16:Ald of sympatric species; and CmedPR3 responded to Z11-18:OH and Z13-18:OH, as well as the interspecific pheromones Z11-16:OH, Z9-16:Ald, Z11-16:Ac, and Z11-16:Ald of sympatric species. Thus, CmedPR2 and CmedPR3 share the ligand Z11-16:Ald, which is not a component of the C. medinalis sex pheromone. Therefore, the sex pheromones of interspecific species affected the input of neural signals by stimulating the sex pheromone receptors on the antennae of male C. medinalis moths, thereby inhibiting the olfactory responses of the male moths to the sex pheromones. Our results demonstrate chemical communication among sympatric species in the rice field habitat, the recognition of intra- and interspecific sex pheromones by olfactory receptors, and how insect premating behaviors are modulated to possibly affect resource partitioning.

Refinement of different combinations of (Brinjal Shoot and Fruit Borer) in Brinjal

BSFB (Brinjal shoot and fruit borer) Lesinoides orbonalis is the most destructive insect for brinjal production. It reduces the value of market price. The experiment comprised eight (8) treatments including one untreated plot. The treatments comprised different rational combinations of mechanical control involving collection and destruction of infested plant parts, use of sex pheromone trap in the centre of the plot and at the canopy level and judicious application of Marshal 20 EC @ 2ml per litter of water based on adult monitoring or routine spray. The treatments differed among themselves in efficacy, and treatment that comprised pheromone trap placed at the plant canopy + mechanical control + spray of Marshal 20 EC @ 2ml per litter of water 2 days interval just from the flowering was adjudged showed significantly lower fruit infestation (different fruiting stage 6.43%, 13.70%, 8.90% respectively) compared to other treatments. Among all the treatment T3 (pheromone trap placed at the plant canopy + mechanical control + spray of Marshal 20 EC @ 2ml per litter of water 2 days interval just from the flowering) was the best option. This treatment also provided the highest benefit cost ratio (2.09) among all the treatments.

Forecasting the insecticide spraying periods of the first generation of Japanese mealybug using sex pheromone trap catches of the overwintering generation of adult males

ISHS VII International Symposium on Persimmon Forecasting the insecticide spraying periods of the first generation of Japanese mealybug using sex pheromone trap catches of the overwintering generation of adult males

Prediction of Tuta absoluta (Meyrick) Generations Using Cumulative Degree-Days Model

Faskha, S.M., R. Darwish and A. Dayoub. 2022. Prediction of Tuta absoluta (Meyrick) Generations Using Cumulative Degree-Days Model. Arab Journal of Plant Protection, 40(4): 299-306. https://doi.org/10.22268/AJPP-40.4.299306 The seasonal changes in the population density of tomato leafminer, Tuta absoluta, and its relationship to cumulative degree-days was studied using a sex pheromone trap in a plastic house located in Tartous Governorate, Syria during the spring season of two successive years 2020 and 2021. The results obtained showed that the male T. absoluta activity extended from late February to the end of the season in July. The highest density for males recorded in May was 207 and 213 males/trap in both seasons, respectively. The calculation of the cumulative degree-days showed that the insect has five generations with a thermal constant that reached in the first season: 516.95, 510.24, 512.2, 563.44, 565.94 DD for each generation, respectively, and in the second season 554.52, 533.06, 557.21, 502.55, 508.22 DD for each generation, respectively, without significant differences between the two seasons (x² = 6.02, p = 0.2 and x² = 4.94, p = 0.29). However, according to the model followed, a simplified table was proposed to calculate the cumulative degree-day instead of using mathematical models, based only on the daily maximum and minimum temperatures. Keywords: Tuta absoluta, degree-days, generations, Tartous, Syria

Deterministic and Stochastic Fractional Order Model for Lesser Date Moth

In this paper, a deterministic and stochastic fractional order model for lesser date moth (LDM) using mating disruption and natural enemies is proposed and analysed. The interaction between LDM larvae, fertilized LDM female, unfertilized LDM female, LDM male and the natural enemy is investigated. In order to clarify the characteristics of the proposed deterministic fractional order model, the analysis of existence, uniqueness, non-negativity and boundedness of the solutions of the proposed fractional-order model are examined. In addition, some sufficient conditions are obtained to ensure the local and global stability of equilibrium points. The occurrence of local bifurcation near the equilibrium points is investigated with the help of Sotomayor’s theorem. Numerical simulations are conducted to illustrate the properties of the proposed fractional order model with respect to the intrinsic growth rate of the LDM larvae, natural enemy’s mortality rate, predation rate, sex pheromone trap parameter, fractional order and environmental noise. The impact of mating disruption on lesser date moth is demonstrated. Also, a numerical approximation method is developed for the proposed stochastic fractional-order model.

Assessment of the Attraction Range of Sex Pheromone Traps to Agriotes (Coleoptera, Elateridae) Male Click Beetles in South-Eastern Europe.

Simple SummaryClick beetles are the adults of wireworms, soil-dwelling larvae that damage multiple arable crops. The attraction range of YATLORf pheromone traps to click beetles of four species of Agriotes wireworms was studied to provide additional information about the implementation of integrated pest management against these harmful pests in Europe. This should allow a significant reduction in insecticide use. Male click beetles were marked and released at different distances from a pheromone trap. Recapture rate, maximum sampling ranges, and effective sampling areas were calculated. The recapture rate was significantly affected by distance, species, and wind direction and decreased as distance increased. The majority of beetles were caught from short distances (up to 10 m) within the first five days. The estimated attraction range was low for all the considered Agriotes species, suggesting that pheromone traps were unsuitable for use as mass trapping instruments to disrupt mating. However, after results and previous research outputs were evaluated, it seems possible to use the traps not only as monitoring tools, but also as attract-and-kill strategies for most beetle populations.The attraction range of YATLORf pheromone traps to adults of four species of Agriotes (A. brevis, A. sordidus, A. litigiosus, and A. ustulatus) was studied to provide additional information about the most harmful Agriotes species in Europe. Male click beetles were marked and released at different distances from a pheromone trap. The recapture rate was calculated and analyzed using analysis of variance. The recapture rate was significantly affected by distance, species, and wind direction. The recapture rate decreased as distance increased. The majority of beetles were caught from short distances (up to 10 m) within the first five days. A. brevis, a mainly crawling species, showed the lowest recapture rate. The wind direction affected the recovery rate, with a significantly lower number of beetles moving downwind from the release points. Maximum sampling ranges and effective sampling areas were calculated. The obtained estimations were low (53 to 86 m and 509 to 2602 m2, respectively) for all the considered Agriotes species, suggesting that they were unsuitable for use as mass trapping instruments to disrupt mating. However, it seems possible to use the traps not only as monitoring tools, but also as attract-and-kill strategies for most beetle populations.

Integrated Pest Management Practices Adopted by Mango Growers at Meherpur Region in Bangladesh

A study on integrated pest management (IPM) practices adopted by the mango growers was carried out at Mujibnagar Upazila under Meherpur district of Bangladesh during August-September 2018 using structured questionnaire. Randomly selected 112 mango growers were used for the study. Results indicated that 75.9% of the respondents were highly adopted with IPM practices during mango cultivation. According to IPM Practices Use Index (IPUI), harvesting at right stage was ranked 1st and use of sex pheromone trap was ranked 10th as commonly used IPM tactic by the growers. The use of logistic regression model in this study was identified as the significant factor of IPM adoption, explore several factors, including contact with IPM club, IFMC and Farmers Field School (FFS) and annual income from mango cultivation. SAARC J. Agric., 19(1): 125-138 (2021)

Appropriate Number of Sex Pheromone Trap for Monitoring Spodoptera exigua Hubner (Lepidoptera: Noctuidae) Moths on Shallot Field

The research was carried out to determine the appropriate number of sex pheromone traps for monitoring the Spodoptera exigua moths in shallot crop which was grown in Yogyakarta Special Territory, Bantul Regency (Samas). This research was conducted in August until September 2016. Sex pheromone trap was arranged on shallot aged 3 weeks after planting. Sex pheromone trap with hexadecenat tetra asetat ingredients was placed on sticky yellow trap. This research used RCBD experimental design with single factor experiment namely the number of trap i.e. 3, 5, 6, 8 units/2.000 m2 with sex pheromone, and three traps without sex pheromone as control. Each treatment was repeated 5 times so 125 samples of experiments were obtained. Observation was made by counting the number of moths caught in sex pheromone trap. The result of observation was analysed with analysis of variance (ANOVA) and DMRT α 5.0%. The results show that the appropriate number of trap to monitor the pest infestation was 3 units/2.000 m2.