Sewer Overflows Research Articles

Addressing underestimation of waterborne disease risks due to fecal indicator bacteria bound in aggregates.

This study aims to identify and address significant limitations in current culture-based regulatory methods used for monitoring microbiological water quality. Specifically, these methods' inability to distinguish between planktonic forms and aggregates containing higher bacterial loads and associated pathogens may lead to a severe underestimation of exposure risks, with critical public health implications. We employed a novel methodology combining size fractionation with ALERT, an automated rapid method for comprehensive quantification of culturable fecal indicator bacteria (FIB). Our findings reveal a substantial and widespread presence of aggregate-bound indicator bacteria across various water matrices and geographical locations. Comprehensive bacterial counts consistently exceeded those obtained by traditional methods by significant multiples, such as an average of 3.4×at the Seine River 2024 Olympic venue, and occasionally up to 100×in irrigation canals and wastewater plant effluent. These results, supported by microscopic and molecular analyses, underscore a systematic bias in global water safety regulatory frameworks. Our research demonstrates the inadequacy of traditional culture-based techniques in assessing microbiological risks posed by aggregate-bound FIB and associated pathogens, particularly in water matrices affected by FIB-rich fecal particles from recent sewer overflows or sediment, which can carry higher infectious risks. Incorporating comprehensive FIB analysis techniques, including molecular methods and rapid culture-based approaches as shown in this study, offers a promising and effective solution to these risk assessment limitations.

The Characteristics and Traceability Analysis of the Overflow Pollution During the Flood Season in an Urban Area

The issue of combined sewer overflow (CSO) triggered by rainfall has become a significant obstacle to the improvement of water environment quality. This study conducted a long-term monitoring of three types of rainwater outlets, i.e., combined sewer overflows (Test-CSO), separated sewer outlets (Test-SSO), and partially separated sewer outlets (Test-PSSO), to reveal the characteristics of overflow pollution and trace its sources by monitoring the pollutants from different underlying surfaces across various urban functional areas. The results showed that the major pollutants in overflow events exhibited the following order: COD ≥ TSS > TN > TAN > TP. Rainwater elevated COD and TSS in the Test-CSO, while reducing nitrogen and phosphorus concentrations by dilution. The Test-PSSO experienced varying degrees of overflow pollution, primarily due to the sewer sediment. A negative relationship between the rainfall and peak time of overflow pollution was observed. The traceability analysis indicated the overall pollution intensity exhibited the following order: residential areas > industrial parks > commercial areas. In addition to commercial areas, the pollution intensity across underlying surfaces generally exhibited the following order: roofs > roads > grasslands. The roof runoff was an important source of pollutants for overflow pollution, and TSS and COD were the major contributors. Notably, grasslands had a buffering effect on pollutants and pH.

Application of Real-Time Control and Source Control Solutions to Reduce Combined Sewer Overflows: A Review of Approaches and Performances

Application of Real-Time Control and Source Control Solutions to Reduce Combined Sewer Overflows: A Review of Approaches and Performances

Sanitary sewer overflows, household sewage backups, and antibiotic-resistant bacteria: the new frontier of environmental health risks and disasters

Abstract Untreated sewage discharges leading to environmental contamination are increasingly common in communities across the globe. The cause of these discharges range from sewer lines in disrepair, blockages, and in the era of more extreme wet weather events, the infiltration of stormwater into the system during heavy downpours. Regardless of the driver of these events, the aftermath results in raw sewage spilling into local waterways, city streets, and commercial and residential structures. Historical research in public health has thoroughly documented the connection between exposure to untreated sewage and waterborne disease. Recent research has detected antibiotic-resistant bacteria at wastewater treatment facilities at a time when deaths by antibiotic-resistant infections are on the rise. However, no research has explored the exposure pathways of antibiotic resistant bacteria during sanitary sewer overflows and household-level sewage backups. In this commentary, we aim to introduce this new frontier of environmental health risks and disasters. To do this we describe the history of modern sanitation and sewer infrastructure with a particular focus on wastewater infrastructure in the U.S. We also explore emerging risks and current methods for identifying antibiotic-resistant bacteria in the environment. We end with future directions for interdisciplinary scholarship at the nexus of urban planning, engineering, and public health by introducing the Water Emergency Team (WET) Project. WET is a community-based multi-method effort to identify environmental risks in the aftermath of household backups through 1) residential surveys, 2) indoor visual inspections, 3) environmental sampling, and 4) laboratory processing and reporting. Our hope is that by introducing this comprehensive approach to environmental risks analysis, other scholars will join us in this effort and ultimately towards addressing this grand challenge of our time.

Application of a hybrid fuzzy-based algorithm to investigate the environmental impact of sewer overflow

PurposeThis study underscores the critical importance of well-functioning sewer systems in achieving smart and sustainable urban drainage within cities. It specifically targets the pressing issue of sewer overflows (SO), widely recognized for their detrimental impact on the environment and public health. The primary purpose of this research is to bridge significant research gaps by investigating the root causes of SO incidents and comprehending their broader ecological consequences.Design/methodology/approachTo fill research gaps, the study introduces the Multi-Phase Causal Inference Fuzzy-Based Framework (MCIF). MCIF integrates the fuzzy Delphi technique, fuzzy DEMATEL method, fuzzy TOPSIS technique and expert interviews. Drawing on expertise from developed countries, MCIF systematically identifies and prioritizes SO causes, explores causal interrelationships, prioritizes environmental impacts and compiles mitigation strategies.FindingsThe study's findings are multifaceted and substantially contribute to addressing SO challenges. Utilizing the MCIF, the research effectively identifies and prioritizes causal factors behind SO incidents, highlighting their relative significance. Additionally, it unravels intricate causal relationships among key factors such as blockages, flow velocity, infiltration and inflow, under-designed pipe diameter and pipe deformation, holes or collapse, providing a profound insight into the intricate web of influences leading to SO.Originality/valueThis study introduces originality by presenting the innovative MCIF tailored for SO mitigation. The combination of fuzzy techniques, expert input and holistic analysis enriches the existing knowledge. These findings pave the way for informed decision-making and proactive measures to achieve sustainable urban drainage systems.

Editorial: Plugging the data gap to enhance standardization in monitoring combined sewer overflows

Editorial: Plugging the data gap to enhance standardization in monitoring combined sewer overflows

Global metagenomic survey identifies sewage-derived hgcAB+ microorganisms as key contributors to riverine methylmercury production

Methylmercury (MeHg) in aquatic systems poses a serious public health risk through bioaccumulation in the aquatic food web. In recent years, MeHg has been observed to increase to concerning levels globally in rivers near cities; however, the causes of this increase are not well understood. Here, we demonstrate the significant role of sewage contamination by analyzing over 1,300 publicly available metagenomes in urban rivers worldwide, and conducting experiments with water samples across China. We find that sewage contamination significantly increases the abundance of mercury (Hg)-methylating microorganisms in urban rivers globally. This increase is primarily attributed to the high abundance of active Hg-methylating microorganisms in sewage, which migrate to rivers via direct discharge or combined sewer overflows (CSOs), becoming key contributors to elevated riverine MeHg levels. Our findings underscore the importance of effectively eliminating Hg-methylating microorganisms from sewage to mitigate the public health risks associated with MeHg in urban rivers.

Retrofitting sustainable drainage systems in UK schools: assessing the impacts and challenges

Sustainable drainage systems (SuDS) mimic natural processes to manage stormwater runoff close to its source, encouraging infiltration, attenuation and passive treatment. SuDS can be used to mitigate flooding and provide increased climate resilience while simultaneously delivering amenity, biodiversity and economic benefits to local communities. Retrofitting SuDS is a complex process requiring a detailed understanding of localised and catchment-wide drainage issues, and necessitates extensive buy-in from multiple stakeholders. Mutual understanding and recognition of the benefits of SuDS retrofit opportunities is critical to their implementation and success. This paper appraises the key benefits and challenges of SuDS retrofit within schools. A qualitative and quantitative assessment using case studies from over 50 completed SuDS retrofit projects within schools across the UK, for a variety of clients, was conducted. Through implementation of these solutions, hydraulic models have demonstrated a reduction in peak flow rates of up to 87%, providing flood alleviation to the schools’ wider catchments and demonstration that SuDS can be used to provide reductions to combined sewer overflow spills. Barriers to implementation may be minimised by ensuring a client’s aims are fully understood and achievable, combined with a focus on stakeholder engagement throughout the decision-making process.

Capacity Assessment of a Combined Sewer Network under Different Weather Conditions: Using Nature-Based Solutions to Increase Resilience

Severe weather conditions and urban intensification are key factors affecting the response of combined sewer systems, especially during storm events. In this regard, the capacity assessment of combined sewer networks under the impact of rainfall storm events of different return periods was the focus of this work. The selected case study area was a mixed-use catchment in the city centre of Thessaloniki, Greece. The hydraulic performance of the examined sewer network was assessed using an InfoWorks ICM model. The results indicated that mitigation strategies, such as the application of nature-based solutions (NBSs) or low-impact developments (LIDs) are considered essential for controlling combined sewer overflows. A multicriteria analysis was conducted to select the most appropriate NBSs/LIDs to be located in the study area to enhance the system’s capacity. The results of this multicriteria analysis were used to propose a combined sewer overflow mitigation scenario, based on the installation of green roofs, as the most highly ranked solution in the analysis performed. Incorporating the proposed NBS/LID in the hydrologic-hydraulic model significantly increased the performance of the studied combined sewer network.

Assessing human-source microbial contamination of stormwater in the U.S

Stormwater capture and use (SCU) projects have the potential to provide a significant portion of municipal water demand. However, uncertainty about the degree of microbial contamination in stormwater and the required treatment is a barrier for implementation of SCU projects. Stormwater runoff could become contaminated with human fecal matter in areas with deteriorating infrastructure where raw wastewater exfiltrates from sewer networks to stormwater collection networks, homeless encampments exist, or sanitary sewer overflows (SSOs) occur. Estimation of human fecal contamination can inform selection of stormwater treatment targets. This study investigates stormwater microbial contamination originating from human fecal matter using observed detections and concentrations of human microbial source tracking (MST) markers and potentially human-infectious pathogens (PHIPs). First, a systematic review complied measurements of human MST markers in wet and dry weather stormwater flows and influent wastewater. In addition, measurements of viral pathogens (e.g., adenoviruses, norovirus GI+GII, and enteroviruses) and protozoan pathogens (e.g., Giardia lamblia and Cryptosporidium parvum) in wet weather flows and influent wastewater were assessed. Human MST marker and PHIP data were statistically analyzed and applied to estimate a human fecal contamination analog (HFCA) which is an estimate of the amount of human fecal matter based on relative concentrations of microbial contaminants in stormwater compared to municipal wastewater. Human MST-based HFCAs in wet and dry weather flows ranged from <10−7.0 to 10−1.5 (median = 10−4.5) and 10−12 to 10−2.6 (median = 10−7.0), respectively. PHIP-based HFCAs in wet weather flows ranged from ∼10−8 to 10−0.14. Estimates of human MST-based HFCAs are more reliable than PHIP-based HFCAs because the current PHIP datasets are generally limited by the number of data points, percent detection, variability observed within the statistical distributions, and the geographical span of sampling locations. The use of human MST-based HFCAs is recommended to guide the selection of stormwater treatment process trains that are protective of public health based on the intended end use. Application of HFCA 10−1 (i.e., sewage dilution 10−1) remains a reasonable conservative estimate of human fecal contamination in stormwater to inform selection of pathogen log reduction targets based on the data presently available.

Sewage leakage challenges urban wastewater management as evidenced by the Yangtze River basin of China

Sewage leakage, including uncollected, collected but exfiltrated, and collected but discharged with stormwater in combined sewer overflows, becomes a key challenge to clean water around the world. This study proposed a mass balance model to quantify sewage leakage and analyzed its influencing factors. Approximately 56% (53%–59%) of domestic total nitrogen loads in the Yangtze River basin of China leak out without treatment, two-thirds are exfiltrated or overflowed, significantly impacting receiving water quality. Cities with higher precipitation have greater leakage, indicating overflows are important. Pipe density has limited impact, showing higher importance of pipe quality over quantity. The per capita leakage rate first increases with city size and then decreases, showing the economies of scale. Yet uneven distribution of population and economy within megacities leads to greater leakage. Hence, separated sewage systems of rainwater and wastewater, regular maintenance and adapting centralized and decentralized systems are recommended, tailored to city-specific leakage characteristics.

Stakeholder perceptions, challenges, and sustainable solutions for achieving safely managed sanitation in Kazakhstan: a case study from a cold region

ABSTRACT In this paper, we present survey results from 30 sanitation stakeholders across the public and private sectors, professional associations, non-governmental organisations (NGOs), and households in the two coldest cities of Kazakhstan. The objectives were to identify the perceived sanitation challenges, document innovative, and traditional solutions employed by the stakeholders to manage sanitation challenges during the cold season, and to propose initiatives and policy interventions to alleviate pressing sanitation issues. Commonly encountered problems included improper installation of septic tanks (e.g. above the frost line) by non-professionals and the difficulty of households to access latrines during heavy snowfall events. Ninety-three percent of respondents expressed that foul odours and sewage overflows were their primary concerns, with only 7% reporting health impacts as a primary concern. There were significant differences in the viewpoints of the respondents regarding the extent of sanitation infrastructure maintenance or upgrades needed in the two cities, suggesting regional differences across the country rather than a uniform national situation. In the context of Kazakhstan, local NGOs were identified as playing a key role in engaging with households to co-devise affordable and sustainable sanitation solutions that are suitable for cold regions, which can then be communicated to other sanitation stakeholders, including the public sector.

The impact of blue-green infrastructure on trace contaminants: A catchment-wide assessment

Blue-green infrastructure (BGI) reduce urban combined sewer overflows (CSOs) and stormwater outlets (SWOs). However, most conventional BGI are not designed to remove trace organic contaminants. Little is known about the potential of conventional BGI to improve surface water quality by reducing the discharge of trace organic contaminants. We derived wash-off loads for street runoff (6PPD-q, DPG, and HMMM), construction materials (diuron), and wastewater-derived contaminants (diclofenac) based on measurements in the combined sewer system. Subsequently, the performance of four BGI types (bioretention cells, green roofs, porous pavements, and urban wetlands) to reduce the discharge of trace organic contaminants via SWOs and CSOs was quantified with a hydrodynamic SWMM model. Moreover, the catchment-wide impact of SWOs and CSOs on surface water was assessed using risk quotients. We found that the annually discharged load can be considerably reduced by implementing BGI. Among the studied BGI types, bioretention cells are the most effective, with a load reduction of up to 80% to surface waters, mainly due to a larger suitable implementation area and a substantial stormwater infiltration. BGI implemented in the separate sewer system are more effective in reducing stormwater contaminant loads than BGI in the combined system. The assessment of the risk quotient in the surface water showed that the concentrations during SWO and CSO discharges exceed the acute environmental threshold in the surface water for 6PPD-q, DPG, diuron, and diclofenac during several events. The implementation of BGI reduced the hours of exceeded risk quotient in the surface water by 93% for bioretention cells. These findings underscore the need for a catchment-wide assessment of future BGI implementations to quantify, manage, and mitigate the impacts of urban pollution.

Unraveling the synergistic mechanisms of coagulation combined with oxidation for the treatment of sewer overflow: The interaction between iron species and NaClO

During wet weather, sewer overflow pollution can pose a serious threat to surface water. In order to reduce the impact of overflow discharge on receiving waters, ferric chloride (Fe(Ⅲ))/potassium ferrate (Fe(Ⅵ))/polyacrylamide (PAM) coagulation (Fe(Ⅲ)/Fe(Ⅵ)/PAM) combined with sodium hypochlorite (NaClO) oxidation was proposed. Different combinations were constructed, including pre-oxidation coagulation (NaClO-Fe(Ⅲ)/Fe(Ⅵ)/PAM), pre-coagulation oxidation (Fe(Ⅲ)/Fe(Ⅵ)/PAM-NaClO), and synchronous coagulation oxidation (NaClO+Fe(Ⅲ)/Fe(Ⅵ)/PAM). The combined processes achieved efficient removal of conventional contaminants, and the produced byproducts were controlled, especially in the NaClO-Fe(Ⅲ)/Fe(Ⅵ)/PAM. The obvious discrepancy in the sulfamethoxazole (SMX) removal was observed in different processes. NaClO affected the distribution of hydrolyzed iron species, and the proportion of active iron in the NaClO-Fe(Ⅲ)/Fe(Ⅵ)/PAM significantly increased. More complexation sites were generated in the NaClO-Fe(Ⅲ)/Fe(Ⅵ)/PAM, which can complex with the coagulant and then effectively transfer to the flocs. The composition of the flocs further confirmed the differences in coagulation characteristics. The generated·OH played a crucial role in SMX removal in the NaClO+Fe(Ⅲ)/Fe(Ⅵ)/PAM, and ClO·was responsible for partial removal of ammonia nitrogen (NH4+-N). The contribution of high-valent iron species was confirmed, and the introduction of NaClO promoted the generation of iron species. This study may provide an ideal for overflow treatment to improve the urban water environment.

Assessment of wastewater ecotoxicity in a large hybrid sewer system in Lodz (Poland)

ABSTRACT Municipal wastewater may contain toxic substances that have a negative impact on biological treatment processes and receiving waters. This paper presents the wastewater toxicity test results from the Lodz hybrid sewage system. Wastewater is discharged to surface waters through a treatment plant, but also during wet weather, partially without treatment by combined sewer overflows. The research was carried out using the ToxTrakTM method with the use of the microbial community of activated sludge from the wastewater treatment plant in Lodz. The median toxicity, expressed as the degree of inhibition, ranged from 16.0% to 30.7%, but sometimes exceeded 50%. Higher toxicity was observed in wet weather than in dry weather. Seasonal variability was found but typical daily variability was not observed. In the case of sewage samples with the degree of inhibition above 40%, a significant correlation was found with chromium concentration and non-ionic surfactants (Pearson’s coefficient correlation above 0.7).

Phosphorus removal in surface flow treatment wetlands for domestic wastewater treatment: Global experiences, opportunities, and challenges

Treatment Wetlands (TWs) are widely used for the treatment of domestic wastewater, with an increasing emphasis on provision of multiple co-benefits. However, concerns remain regarding achieving stringent phosphorus (P) discharge limits, system robustness and resilience, and associated guidance on system design and operation. Typically, where P removal is intended with a passive TW, surface flow (SF) systems are the chosen design type. This study analysed long-term monitoring datasets (2–30 years) from 85 full-scale SF TWs (25 m2 to 487 ha) treating domestic sewage with the influent load ranging from 2.17 to 54,779 m3/d, including secondary treatment, tertiary treatment, and combined sewer overflows treatment. The results showed median percentage removals of total P (TP) and orthophosphate (Ortho P) of 28% and 31%, respectively. Additionally, median areal mass removal rates were 5.13 and 2.87 gP/m2/yr, respectively. For tertiary SF TWs without targeted upstream P removal, 80% of the 44 systems achieved ≤3 mg/L annual average effluent total P. Tertiary SF TWs with targeted upstream P removal demonstrated high robustness, delivering stable effluent TP < 0.35 mg/L. Seasonality in removal achieved was absent from 85% of sites, with 95% of all systems demonstrating stable annual average effluent TP concentrations for up to a 30-year period. Only two out of 32 systems showed a significant increase in effluent TP concentration after the initial year and remained stable thereafter. The impact of different liner types on water infiltration, cost, and carbon footprint were analysed to quantify the impact of these commonly cited barriers to implementation of SF TW for P removal. The use of PVC enclosed between geotextile gave the lowest additional cost and carbon footprint associated with lining SF TWs. Whilst the P-k-C* model is considered the best practice for sizing SF TWs to achieve design pollutant reductions, it should be used with caution with further studies needed to more comprehensively understand the key design parameters and relationships that determine P removal performance in order to reliably predict effluent quality.

Ecotoxicological assessment of sanitary sewer overflows and rainfall dynamics offers insights into conditions for potential adverse ecological outcomes

Sewer overflows are an environmental concern due to their potential to introduce contaminants that can adversely affect downstream aquatic ecosystems. As these overflows can occur during rainfall events, the influence of rainwater ingress from inflow and infiltration on raw untreated wastewater (influent) within the sewer is a critical factor influencing the dilution and toxicity of the contaminants. The Vineyard sewer carrier in the greater city of Sydney, Australia, was selected for an ecotoxicological investigation of a sanitary (separate from stormwater) sewerage system and a wet-weather overflow (WWO). Three influent samples were collected representing dry-weather (DW), intermediate wet-weather (IWW) and wet-weather (WW). In addition, a receiving water sample was also collected downstream in Vineyard Creek (WW-DS) coinciding with a WWO.We employed direct toxicity assessment (DTA) and toxicity identification evaluation (TIE) approaches to gain comprehensive insights into the nature and magnitude of the impact on influent from rainwater ingress into the sewer. Three standard ecotoxicological model species, a microalga, Chlorella vulgaris, the water flea, Ceriodaphnia dubia and the midge larva, Chironomus tepperi were used for both acute and chronic tests. The study revealed variable toxicity responses, with the sample of influent collected in wet-weather displaying lower toxicity compared to the dry-weather sample of influent. Ammonia, and metals, were identified in dry weather as contributors to the observed toxicity, however, this risk was alleviated through rainwater ingress in wet-weather with further dilution within the receiving water. Based on toxicity data, dilutions of influent to minimise effects on C. vulgaris and C. dubia ranged from 1 in 12 in DW to 1 in 2.8 in WW, and further diminished in the receiving water to 1 in 1.8. The successful application of ecotoxicological approaches enabled the assessment of cumulative effects of contaminants in influent, offering valuable insights into the sanitary sewer system under rainwater ingress.

Microplastic removal and risk assessment framework in a constructed wetland for the treatment of combined sewer overflows

Combined sewer overflows (CSOs) release a significant amount of pollutants, including microplastics (MPs), due to the discharge of untreated water into receiving water bodies. Constructed Wetlands (CWs) offer a promising strategy for CSO treatment and have recently attracted attention as a potential solution for MP mitigation. Nevertheless, limited research on MP dynamics within CSO events and MP removal performance in full-scale CW systems poses a barrier to this frontier of application. This research aims to address both these knowledge gaps, representing the first investigation of a multi-stage CSO-CW for MP removal. The study presents one year of seasonal data from the CSO-CW upstream of the WWTP in Carimate (Italy), evaluating the correlation of MP abundance with different water quality/quantity parameters and associated ecological risks. The results show a clear trend in MP abundance, which increases with rainfall intensity. The strong correlation between MP concentration, flow rate, and total suspended solids (TSS) validates the first flush phenomenon hypothesis and its impact on MP release during CSOs. Chemical characterization identifies acrylonitrile-butadiene-styrene (ABS), polyethylene (PE), and polypropylene (PP) as predominant polymers. The first vertical subsurface flow (VF) stage showed removal rates ranging from 40 % to 77 %. However, the unexpected increase in MP concentrations after the second free water surface (FWS) stage suggests the stochasticity of CSO events and the different hydraulic characteristics of the CW units have diverse effects on MP retention. These data confirm filtration as the main retention mechanism for MP within CW systems. The MP ecological risk assessment indicates a high-risk category for most of the water samples, mainly related to the frequent presence of ABS fragments. The results contribute to the current understanding of MPs released by CSOs and provide insights into the performance of different treatment units within a large-scale CSO-CW system, suggesting the requirement for further attention.

Modelling an urban wastewater system via a space–time multivariate calibration to understand and improve water bodies quality

ABSTRACT The European Water Framework Directive requires good quality status for water bodies remaining solely attainable through a global approach. Urban wastewater integrated modelling is a promising tool for this purpose. However, certain barriers still hinder its application, such as the significant amount of data required and specifically in the sewer system, the issues with data quality or the monitoring and maintenance costs. This study develops an integrated model for Dijon Metropole in France using multiple hydraulic and quality data sources (e.g. sensors, analysers, laboratory measurements) and calibration criteria. The results show that long-term trends can be predicted for hydraulic and quality variables. The scenarios demonstrate that combined sewer overflows are responsible for most of the anoxic and ammonium high concentration periods in the rivers during rain events compared to the Water Resources Recovery Facility (WRRF). Therefore, adding storm tanks in the wastewater system will reduce stress on aquatic organisms during rainfall events. Reducing WRRF discharge during wet weather can also reduce the average and percentile of ammonium in the river. These results indicate that strategies should be adapted depending on the water quality objective (biological and/or physico-chemical) and the sensitivity of the organisms present in the river.

Impact of forced aeration on vertical flow treatment wetland performances for combined sewer overflow

Combined Sewer Overflow Treatment Wetlands (CSO wetlands) are designed to remove pollutants under stochastic events with variable hydraulic loads. Upgrading them with forced aeration promises to increase the effectiveness and resilience of the treatment. We have tested two vertical CSO wetlands with forced aeration (CSOa and CSOb) to understand the effects of aeration on CSO treatment. Both filter beds have 0.95 and 0.80 m of saturated layer. CSOa uses gravel as top filtering layer, while CSOb utilizes sand and an additional transition layer. Tracer tests were conducted in both filters with and without aeration to assess the impact of aeration on hydraulic performance. CSOa operated with four different aeration conditions, with the optimal condition tested on both filters for comparison. Samples were taken for analysis of global parameters and the redox potential was monitored online. In the tracer test, CSOa allowed to observe the mixing impact of aeration, which avoids any preferential path when influent entered the filter. The addition of a sand layer at the surface (CSOb) allows for a more even distribution of water on the top, which limits preferential flows when aeration is off. In both filters, the results showed that aeration increased the residence time and mixing degree (NTIS <3). Testing different aeration strategies revealed the dependence of dissolved pollutant removal on oxygen supply. In CSOa, the median outlet concentration varied from 23 to 6.4 mg.L−1 in TSS, 153 to 32 mg.L−1 total COD (CODt), 124 to 20 mg.L−1 soluble COD (CODs) and 5 to 2.5 mg.L−1 NH4-N according to aeration strategy. The lower outlet concentrations were always under the highest aeration condition. Under the optimal condition (75 min on/15 min off), median removal of CSOa was 97% TSS, 85% CODt, 78% CODs and 75% NH4-N. Besides COD and TSS, outlet concentration and removal efficiency did not significantly differ between CSOa and CSOb. Pollutant removal demonstrated a linear correlation with organic surface load. Overall, forced aeration in CSO-TW distinctly affected filter dynamics and improved its performance.