Severity Of Angular Leaf Spot Research Articles

Effects of botanical extracts and antibiotic on incidence and severity of bacterial blight of cotton incited by Xanthomonas axonopodis pv. Malvacearum

A multilocational field trial was conducted to investigate the effect of some plant extracts (Bolanite aegyptica, Eucalyptus camaldulensis and Citrus aurantium) and a synthetic antibiotic (Streptomycin) on the incidence and severity of angular leaf spot on five cotton geno-types namely SAMCOT-8, SAMCOT-9, SAMCOT-10, SAMCOT-11 and SAMCOT-12 in Yola and Jalingo, both located in the northern guinea savannah zone of Nigeria. The experiment was carried out on a split-plot design having three replications with cotton genotypes on the main plots and plant extracts on the sub-plots. Data collected on the incidence and severity of the disease were analysed using the Generalized Linear Model procedure and means separation at 5% level of probability by Duncan Multiple Range Test. Findings of the study revealed that Streptomycin and Bolanite aegyptiaca were able to reduce the disease incidence by 18.12% from 58.69% in Yola at 13 WAS. In Jalingo however, B. aegyptiaca was found to have re-duced the disease severity from 52.12% to 19.69% at 13 WAS. SAMCOT-8 consistently recorded lower incidence and severity means in Yola and Jalingo of 47.96%; 59.12% and 40.82%; 39.70% while SAMCOT-12 recorded higher means of 53.29%; 59.82% and 48.03%; 46.70% respectively.

Efecto del extracto de alga Ulva fasciata sobre Pseudocercospora griseola en el cultivo de frijol

La mancha angular es una de las enfermedades foliares más comunes del cultivo de frijol en el sur de Brasil. El objetivo del trabajo fue evaluar el efecto del extracto de la macroalga Ulva fasciata en la severidad de la mancha angular, los niveles de pigmentos foliares y la productividad de los genotipos de frijol común IPR Uirapuru, CF 22 y Mouro graúdo, durante los años 2005 y 2006. Las plantas tratadas con el extracto de alga o el fungicida azoxistrobina se pulverizaron en los estados fenológicos V3, V5, R6 e R7. Plantas no tratadas se utilizaron como testigos. La severidad (%) de la enfermedad se estimó a partir del estado V3, para un total de seis evaluaciones cada año. Los valores de la severidad se calcularon como área bajo la curva de progreso de la enfermedad (ABCPE). En el 2006 se determinaron los pigmentos foliares (clorofila a, b, total y carotenoides) y en los dos años se evaluó el rendimiento de granos, aunque en el 2005 solamente se evaluó el peso de mil granos. En conclusión, el control que ejerce el extracto de U. fasciata sobre la mancha angular depende de la interacción biológica entre el genotipo de frijol y el ambiente. Por otro lado, el extracto no afectó el contenido de los pigmentos foliares ni la producción de granos.

Increasing the efficiency of recurrent selection for angular leaf spot resistance in common bean

AbstractA common bean (Phaseolus vulgaris L.) breeding program for resistance to Pseudocercospora griseola (Sacc.) Crous & U. Braun (angular leaf spot) using recurrent selection has been conducted since 1998 in Brazil. The selection has been carried out in the field with high natural occurrence of the pathogen. One recurrent selection cycle has been conducted per year. The efficiency of artificial inoculation of P. griseola and early assessment of angular leaf spot severity in common bean progenies of this program were evaluated. For this, 254 and 166 S0:1 progenies from Cycles XVI and XVIII, respectively, were assessed for angular leaf spot severity in field, with natural occurrence of the pathogen and in greenhouse with artificial inoculation of P. griseola. Most progenies showed a high level of resistance to angular leaf spot, indicating the efficiency of recurrent selection. Artificial inoculation of P. griseola in the greenhouse showed promise for the selection of resistant plants in a recurrent selection program allowing breeders to carry out two cycles per year. A scheme was proposed that combined the advantages of early‐generation selection with artificial inoculation of the pathogen in greenhouse with the advantages of selection in field with natural occurrence of P. griseola in the dry season, increasing genetic gain per unit time.

Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance

ABSTRACT: Phenotyping for severity of angular leaf spot (ALS) in common bean is important to identify new sources of resistance and select progenies in conventional and molecular marker-assisted breeding. In this study, three phenotyping methods for ALS severity were assessed and its implications in breeding for resistance to Pseudocercospora griseola are discussed. Reaction of 144 common bean lines to P. griseola was evaluated. Three different experiments were conducted in the greenhouse (V2 and V3 stages) and in the field. Common bean lines were inoculated with a mixture of spores of pathogen races 63-63 and 63-23. We observed that 31 %, 7 % and 10 % of lines were resistant in stages V2, V3 and in the field, respectively. Estimates of coincidence index V2-V3, V2-field and V3-field were 68 %, 69 % and 88 %, respectively. Evaluations in V3 stage and in the field were the phenotyping methods more efficient for ALS severity. However, evaluation in V3 stage is impracticable to assess routinely many genotypes in breeding programs. Evaluation in V2 stage may be used to carry out a first screening, especially in the early stages of breeding programs. Moreover, this methodology may be used in association with evaluation of plants in the field to increase genetic gain.

Management of Snap Bean Insect Pests and Diseases by Use of Antagonistic Fungi and Plant Extracts

Use of synthetic pesticides reduces the competitiveness of Kenyan snap bean pods due to stringent regulations by importers as a result of presence of chemical residues. This study was conducted to determine the effectiveness of local biopesticides in managing insect pests and diseases of snap beans. Field experiments were set up in farmer's field where Trichoderma spp. and Paecilomyce spp. and plant extracts from turmeric, garlic, ginger and lemon were applied weekly as foliar sprays. Plant extracts reduced the population of whiteflies and thrips by up to 58% and 41% while antagonistic fungi had a corresponding 30% and 18% reduction, respectively. Trichoderma spp. reduced severity of angular leaf spot (37.5%), rust (67%) and anthracnose (20.7%). Plant extracts and antagonistic fungi increased marketable pod yield by 25.6% and 17.3%, respectively. Results demonstrated that local environments are potential sources of biopesticides that can be exploited for integrated management of pests and diseases.

Diallel analysis to choose parents for black bean (Phaseolus vulgaris L.) breeding.

In this study, conducted in two different seasons, we aimed to choose parents to obtain promising segregating populations for the extraction of black bean (Phaseolus vulgaris L.) lines that are superior in terms of disease resistance, plant architecture, and grain yield. Twelve parents were arranged in two groups to compose a partial diallel in a 5 x 7 scheme. Group 1 was composed of parents with black grains and erect plant architecture, while group 2 was composed of parents that had carioca grains and were resistant to the main fungal diseases that occur in the common bean. The following traits were evaluated: severity of angular leaf spot (ALS), plant architecture (PAG), and grain yield (YIELD). The data were analyzed according to a partial diallel model using parents and F1 hybrids. In the genetic control of ALS and PAG, additive effects were predominant, while for YIELD, additive effects were predominant in one season and dominance effects were in another season, because it is a more complex trait than ALS and PAG. For YIELD, we observed an interaction between general combining ability and specific combining ability between seasons. The genes that control ALS, PAG, and YIELD were in eight of the 12 parents evaluated in the diallel. The cultivar 'BRS Estilo' is suitable to use as a parent in common bean breeding in terms of ALS, PAG and YIELD. Recurrent selection is the most recommended option for simultaneously breeding for PAG, YIELD, and resistance to angular leaf spot in bean culture.

Diagrammatic scale to evaluate angular leaf spot severity in primary leaves of common bean

The aim of this study was to develop a diagrammatic scale to evaluate angular leaf spot (ALS) severity in primary leaves of common bean. A diagrammatic scale was developed and validated with nine levels of severity: 0 %; 0.1 to 0.5 %; 0.6 to 4.0 %; 4.1 to 7.0 %; 7.1 to 16 %; 16.1 to 26 %; 26.1 to 32 %; 32.1 to 38 % and 38.1 to 60 %. The use of this scale, associated with the early inoculation method, allowed rating of ALS severity in common bean leaves in the V2 stage of development that is, when the primary leaves are fully open. Validation of the scale was performed by 14 Evaluators. These Evaluators estimated the severity in 40 common bean leaves with different levels of severity, previously measured by the software Quant®. The accuracy and precision of each Evaluator were determined by simple linear regression, by comparing between actual severity, measured electronically, and that estimated by the Evaluator. Without the scale, most of the Evaluators overestimated the severity of the disease. With the scale, the Evaluators obtained better levels of accuracy and precision, with absolute errors less than 5 %. The diagrammatic scale proposed proved to be adequate for early performance of the pathogenicity test.

Effect of Some Environmental Factors on Incidence and Severity of Angular Leaf Spot of Cotton in Yola and Mubi, Adamawa State, Nigeria

Environmental factors such as relative humidity and rainfall generally have been found to increase the incidence, rate of spread and severity of diseases thereby reducing yield of crops. Study was conducted on five cotton varieties, which were artificially inoculated with bacterial blight pathogen to determine the effects of rainfall and relative humidity on incidence and severity of angular leaf spot (ALS) and yield of seed cotton in Yola and Mubi. Results showed that the severity of ALS was higher in Yola (58.65%) at 13 WAS assumed to be due to higher relative humidity range of 76% - 87% and low rainfall of 2 - 40.6 mm. This is assumed to have favoured disease development as against that of Mubi location which recorded lower severity (51.11%) due to lower relative humidity (42% - 55%) and rainfall (37 - 73 mm). Results further revealed that at 13 WAS, SAMCOT-8 had low incidence (66%) and severity (39%) in Yola. This was against the much higher corresponding incidence and severity of 82% and 42% respectively that was observed in Mubi during the same period. SAMCOT-10 and SAMCOT-9 varieties were found to be highly susceptible to the disease at the same period. SAMCOT-8 recorded the highest yield of 390.00 kg?ha?1 in Yola and 868.09 kg?ha?1 in Mubi while the lowest yields of 227.17 kg?ha?1 was observed on SAMCOT-10 in Yola while 461.61 kg?ha?1 was obtained on SAMCOT-9 in Mubi. The variation in yield among these varieties might be due to the differences in their reactions to the disease. There is a need to conduct further trials in these locations to confirm the level of resistance or other aspects of these varieties to the disease.

Methodology to Improve the Efficiency of Evaluation of the Severity of Angular Leaf Spot in Common Bean

Disease severity assessment by means of a scoring scale, especially for angular leaf spot (Pseudocercospora griseola) in common bean, is hindered in experiments for assessment of progenies and/or breeding lines due to lack of uniformity of occurrence of the pathogens and segregation within progenies. The purpose of this study was to estimate the efficiency of the use of one plant per plot in assessing the severity of angular leaf spot in experiments for assessment of progenies and/or breeding lines in the common bean crop. To that end, two experimental strategies were used – one of them using one plant per plot and another using a standard size plot (SPP) (2–4-m length rows). The experiments were conducted in the period from November 2011 to May 2012 in the municipalities of Lavras and Lambari, state of Minas Gerais, Brazil. Forty-one lines from the breeding programme of the Universidade Federal de Lavras (UFLA) and from other research institutions were assessed, which differed in regard to their degree of susceptibility to P. griseola. The lines were assessed in regard to the severity of said disease using a five-degree diagrammatic scale. In all the one plant per plot experiments, severity scores of angular leaf spot from the beginning of its occurrence, and later in intervals ranging from 7 to 12 days, were obtained. In the experiment with the SPP, assessment was made a few days prior to grain harvest. Estimates of the correlations between severity scores and grain yield (GY) were mostly of small magnitude. There was good coincidence between the lines classified as more resistant or more susceptible to the pathogen under the two conditions.

Evaluation efficiency of severity of angular leaf spot in common bean based on diseased and healthy leaf area

This study compared severity of angular leaf spot in common bean lines, based on the healthy and diseased leaf area, and the graded scale. We used 12 common bean lines in the dry and rainy seasons. Two contiguous experiments were conducted in each season, with and without chemical control of the pathogen. We evaluated the percentage of the healthy and diseased leaf area; severity based on a graded scale and the area under the disease progress curve; and yield. The diseased or healthy leaf area is efficient to evaluate the severity of angular leaf spot with a sample of 20 to 30 leaflets per plot. For all traits, the results of central and border areas did not differ, indicating that the evaluation of border rows is unnecessary and, finally, the severity assessment of the upper plant half can discriminate the lines more efficiently.

Controle de mancha-angular utilizando-se caldas fertiprotetoras em cultivo orgânico de feijoeiro irrigado

O objetivo deste trabalho foi avaliar a eficácia de caldas fertiprotetoras, em dois intervalos de aplicação, no controle de mancha-angular causada por Phaeoisariopsis griseola, em sistema orgânico de produção, no período de inverno e sob irrigação por aspersão. O experimento foi desenvolvido a campo, com as cultivares de feijoeiro comum (Phaseolus vulgaris L.) Pérola e BRS Valente. Utilizou-se delineamento experimental de blocos ao acaso, em esquema fatorial 2x4, com quatro repetições, em que o primeiro fator correspondeu aos intervalos de aplicação (15 e 30 dias) e o segundo a caldas fertiprotetoras (bordalesa, sulfocálcica e calda elaborada com silicato Rocksil®) e testemunha sem controle da doença. Para a cultivar Pérola, apesar de a eficácia de controle da doença ser semelhante à da calda sulfocálcica, somente a calda bordalesa proporcionou acréscimo no rendimento de grãos de 17,7%, em relação à testemunha, propiciando rentabilidade de R$ 386,40 por hectare. Para a cultivar BRS Valente, as caldas bordalesa e sulfocálcica, aplicadas quinzenalmente, proporcionaram acréscimo ao rendimento de grãos de 25,0% e 21,8%, em relação à testemunha, além de apresentarem eficácia de controle de 62% da mancha-angular, propiciando rentabilidade de R$ 573,15 e R$ 464,10 por hectare, respectivamente.

CONTROLE DE MANCHA-ANGULAR UTILIZANDO-SE CALDAS FERTIPROTETORAS EM CULTIVO ORGÂNICO DE FEIJOEIRO IRRIGADO

A field experiment was carried out with the Pérola and BRS Valente cultivars, aiming to evaluate the efficiency of protective mixtures, in two spraying periods, for controlling angular leaf spot caused by Phaeoisariopsis griseola in common bean, under organic cropping system, in the dry season and under irrigation. A randomized complete block design, in a 2x4 factorial scheme, with four replications, was used, in which the first factor was the spraying intervals (15 and 30 days), and the second the protective mixtures (bordeaux, lime sulfur, and a mixture with silicate Rocksil®) and control without any mixture. To the Pérola cultivar, the bordeaux mixture provided an increase of 17,7% in grain yield, when compared to the non-sprayed control, reducing significantly the angular leaf spot severity, achieving a control efficiency of 75%, and propitiating a profit of R$ 386.40 ha-1, when applied at each 15 days. To the BRS Valente cultivar, the bordeaux mixture and lime sulfur provided an increase of 25% and 21,8% in grain yield, in comparison to the non-sprayed control, achieving a control efficiency of 62% and a profit of R$ 573.15 ha-1 and R$ 464.10 ha-1, respectively, when applied at each 15 days. KEY-WORDS: Phaeoisariopsis griseola; Phaseolus vulgaris; organic agriculture; bordeaux mixture; lime sulfur.

FOLIAR SPRAY OF POTASSIUM SILICATE ON THE CONTROL OF ANGULAR LEAF SPOT ON BEANS

This study aimed to determine if potassium silicate (KSi) sprays could reduce the intensity of angular leaf spot. In field experiment 1, bean plants were sprayed with KSi (pH 10.5) at rates of 8, 20, 40, and 60 g L−1. In field experiment 2, with the same treatments, the pH of the KSi solutions was 5.5. In experiment 3, the treatments were KSi (40 g L−1, pH 5.5), potassium hydroxide (KOH) (6.5 g L−1, pH 5.5), tebuconazole (0.5 L ha−1), and control. In experiment 4, the treatments were the same as in experiment 3, but the pH of the KSi and KOH solutions was 10.5. Plants sprayed with water served as a control treatment for all field experiments. Plants were artificially inoculated with Pseudocercopora griseola before products application. For experiments 3 and 4, the treatment with KOH was included to equalize the amount of potassium (K) with the treatment corresponding to the application of KSi. Disease severity was evaluated using a scale with values ranging from 0.2 to 30.4% at 85 days after seedling emergence. Plant defoliation, Si, and K concentration in the plant tissues, and yield were also determined. There was no relationship between KSi rates and Si concentration in leaf tissues, but Si concentration increased by 58 and 57%, respectively, as the KSi rates increased from 0 to 60 g L−1 regardless of the pH. The K concentration in leaf tissues did not change among the treatments. Disease severity decreased by 42 and 30%, respectively, at the highest KSi rate with pH 5.5 and pH 10.5 over the control. Disease severity levels were similar between the KSi and KOH treatments, but they were significantly higher compared to the tebuconazole. Plant defoliation at the highest KSi rate with pH 5.5 and 10.5 was 17 and 33%, respectively, less than the control. Plant defoliation decreased with the application of KSi with pH 5.5 and 10.5 in 29 and 34%, respectively, compared to the control. Yield increased by 30 and 43%, respectively, as the KSi rates increased from 0 to 60 g L−1with pH 5.5 and 10.5. No statistical difference in yield was detected between the KSi and KOH treatments regardless of the pH used, but both were different from tebuconazole. Significant differences in yield were found only between tebuconazole and the control. Results from this study suggest that foliar application of KSi and KOH has the potential to reduce angular leaf spot severity. The KSi did not offer any advantage over the KOH spray, suggesting a lack of a direct effect of Si accumulated in the leaf tissue on disease control.

Assessment of resurgence of bacterial blight and its effect on cotton yield in northern Nigeria

Cotton production in Nigeria causes fluctuating socio-economic and biotic factors. Bacterial blight induced by Xanthomonas campestris pv malvacearum causes the greatest yield loss annually. A study was therefore carried out to investigate the resurgence of the disease on 10 different cotton genotypes in Dowaya, Kem and Ngurore which are the major cotton areas of Adamawa state of Nigeria under field conditions. Other objectives were to determine the relationship between the different manifestations of bacterial blight and the yield of seed cotton and to identify resistance in the selected genotypes to the disease manifestations. Results revealed the presence of angular leaf spot, vein and boll rot manifestations of the disease in the study areas. The absence of vein blight symptoms in the Kem location did not result in higher yield because the severity of the leaf spot for this location was relatively higher than for the other two locations. Results also revealed that despite the high severity of angular leaf spot, high boll rot and vein blight incidences observed particularly on SAMCOT-11, SAMCOT-13 and ex-Benin in these locations with a high yield of seed cotton was recorded. The three multi-adversity resistant genotypes (MAR), TX-CDP37HH-1-83, TAMCOT SP-21S and TAMCOT CAMD-E recorded relatively lower severity and incidences in both cases. There was, however, a significantly negative correlation between angular leaf spot severity, boll rot incidence and yield at Kem location, significant negative correlation between angular leaf spot severity, boll rot incidence, vein blight and yield at Dowaya location, as well as significant negative correlation between angular leaf spot severity, vein blight and yield at Ngurore location. The tolerant and high yielding SAMCOT-11, SAMCOT-13 and ex-Benin should through breeding work be improved to be used for production in this area and its surroundings.

Suppressive effects of composts on soil-borne and foliar diseases of French bean in the field in the western Indian Himalayas

The high incidence of various soil-borne and foliar diseases is a major production constraint in the profitable cultivation of French bean. Compost and compost teas have been widely explored as an eco-friendly option for controlling plant diseases in container mixes. However, there is limited information regarding their disease suppressive potential under field conditions especially against diseases of French bean. The aim of this study was to investigate the suppressive potential of six composted substrates (farmyard manure, poultry manure, vermicompost, spent mushroom compost, Lantana camara and Urtica sp.), in comparison to a recommended chemical fungicide (carbendazim), against soil-borne and foliar diseases of French bean under the conditions of western Indian Himalayas. The field experiment was conducted over two growing seasons and the composts applied as a soil amendment and later as foliar sprays of their fermented extracts. There was a high level of root rot suppression (>33%) in the poultry manure, Lantana and Urtica compost treatments in both experimental years and these treatments were on par with the chemical seed treatment. All the compost treatments showed a moderate but significant reduction (>20%) in angular leaf spot severity also but none was comparable to that of chemical sprays. There was considerable variability in the suppressiveness of different composts. Overall, poultry manure, composted Urtica sp. and composted Lantana camara treatments were superior to other composts in suppressing both Rhizoctonia root rot ( Rhizoctonia solani Kühn) and angular leaf spot ( Phaeoisariopsis griseola (Sacc.) Ferraris). The different compost treatments exerted a significant effect on the population of Trichoderma spp. and fluorescent pseudomonads in the plant rhizosphere. The highest population of these antagonists was recovered from poultry manure and Urtica compost treatments indicating that it may be a major factor contributing towards the higher disease reduction observed in these treatments. All composts increased yield over the control with maximum yields recorded in poultry manure and Urtica treatments. Although Lantana compost suppressed disease, the yield levels in this treatment were quite low indicating that the compost may be phytotoxic. Our results suggest that application of composts and compost extracts derived from poultry manure and Urtica sp. has the potential to provide effective control of diseases and improve yield in bean under field conditions.

Occurrence and Severity of Angular Leaf Spot of Common Bean in Kenya as Influenced by Geographical Location, Altitude and Agroecological Zones

Occurrence and Severity of Angular Leaf Spot of Common Bean in Kenya as Influenced by Geographical Location, Altitude and Agroecological Zones

Linhagens de feijão do cruzamento 'Ouro Negro' x 'Pérola' com características agronômicas favoráveis

O objetivo desse trabalho foi avaliar e caracterizar, em casa de vegetação e campo, linhagens do cruzamento entre as cultivares Ouro Negro e Pérola, quanto à reação às principais raças de Colletotrichum lindemuthianum e Uromyces appendiculatus. Quatrocentas progênies F7:8, de 40 famílias F3:7 previamente selecionadas na população 'Ouro Negro' x 'Pérola', foram pulverizadas com uma suspensão contendo a raça 89 de C. lindemuthianum. Linhagens resistentes à raça 89 receberam inóculo com as raças 73 e 81 de C. lindemuthianum e com mistura de seis raças de U. appendiculatus. Nas avaliações em campo, realizadas em Viçosa e Coimbra, MG, um látice quadrado triplo 9x9 foi utilizado e foram avaliados produtividade de grãos, severidade de mancha-angular e aspecto do grão. Foram identificadas 42 linhagens resistentes às raças de C. lindemuthianum e U. appendiculatus. Foram selecionadas dez linhagens de grãos tipo 'Carioca', com produtividade igual à da cultivar Pérola e resistentes à antracnose, ferrugem e mancha-angular.

A Semi-Selective agar medium to detect the presence of Xanthomonas axonopodis pv. malvacearum in naturally infected cotton seed

A semi-selective agar medium was developed for detection of Xanthomonas axonopodis pv. malvacearum (Xam) in cotton (Gossypium hirsutum) seed. The basic medium was peptone-sucrose-agar (PSA). Criteria for the semi-selective medium were the typical colony characters of Xam and its pathogenicity on cotton. Several systemic fungicides and antibiotics in different concentrations were tested alone or in combination with others. The final composition of the semi-selective agar medium was established after several attempts in order to inhibit most of the fungal and bacterial saprophytes and favour the development of Xam. It contained PSA + cyclohexamide, cephalexin, pencycuron, triadimenol and tolylfluanid. The bacteria were recovered from naturally infected seeds by the direct plating of 2,000 surface disinfected seeds on the semi-selective medium. The recovery of the pathogen from naturally infected leaf tissues and in dilution plating, on semi-selective medium and on nutrient agar, were comparable. Among the three detection methods tested, the semi-selective medium was found to be the most reliable and quantifiable. Degree of severity of angular leaf spot in the field was not always correlated with the level of infection in the seed. This is the first report of a semi-selective agar medium to detect the presence of Xam in naturally infected cotton seed.

Influência da severidade de mancha angular na eficiência fotossintética da área foliar sadia de feijoeiro, sob condições de campo

This paper aimed to show the relationship among healthy leaf area, angular leaf spot severity and bean yield, under field conditions, indicating the influence of this relationship on photosynthetic efficiency of leaf tissue remaining on plants. Leaf area and severity of angular leaf spot [Phaeoisariopsis griseola (Sacc.) Ferraris] of bean were weekly assessed, beginning at 30 days after emergence (DAE). Different levels of severity were reached varying the timing of fungicide spraying (fentin hydroxide 0.10% + tebuconazole 0.04%). Variations on total leaf area were consequence of natural variation on plants and also due to different physic and chemical soil conditions among plots. The relation between healthy leaf area duration (HAD) and area under disease progress curve (AUDPC) and yield (g m-2) presented R2=74.8% and healthy area absorption (HAA), AUDPC and yield (g m-2) presented R2=74.7%. Results indicated reduction of yield when severity (AUDPC) was high, even in plots with same level of healthy leaf area. So, the disease influenced the photosynthetic efficiency of healthy leaf area, besides avoiding the production of the necrotic tissue.

Quantitative analysis of two important epidemiological features of the common bean: Phaeoisariopsis griseola pathosystem

This work quantifies two important epidemiological features of the bean (Phaseolus vulgaris)/Phaeoisariopsis griseola pathosystem. The first is the effect of the number of nights of leaf wetness on infection efficiency. Infection efficiency was below 10% when inoculated leaflets were exposed to less than two nights of leaf wetness. Optimum infection efficiencies were obtained after three to four nights of leaf wetness, at about 50%. Further nights of leaf wetness did not increase the infection efficiency. The second feature quantified is the relative rate of leaflet defoliation for varying levels of angular leaf spot severity. It increased with disease severity according to a logarithm-like curve, and a relative rate of 0.23 day-1 was estimated for a severity of 18%. The implications of these results on the disease epidemiology are discussed.