Serum Melatonin Research Articles

Circadian Gene Expression Rhythms During Critical Illness.

Core clock genes regulate tissue-specific transcriptome oscillations that synchronize physiologic processes throughout the body, held in phase by the central circadian rhythm. The central circadian rhythm rapidly dampens with onset of critical illness, but the effect of critical illness on gene expression oscillations is unknown. The objective of this study was to characterize the rhythmicity and phase coherence of core clock genes and the broader transcriptome after onset of critical illness. Cross-sectional study. ICUs and hospital clinical research unit. Critically ill patients within the first day of presenting from the community and healthy volunteers. Usual care (critically ill patients) and modified constant routine (healthy volunteers). We studied 15 critically ill patients, including 10 with sepsis and five with intracerebral hemorrhage, and 11 healthy controls. The central circadian rhythm and rest-activity rhythms were profiled by continuous wrist actigraphy, and serum melatonin sampled every 2 hours along with whole blood for RNA isolation over 24 hours. The gene expression transcriptome was obtained by RNA sequencing. Core clock genes were analyzed for rhythmicity by cosinor fit. Significant circadian rhythmicity was identified in five of six core clock genes in healthy controls, but none in critically ill patients. TimeSignature, a validated algorithm based on 41 genes, was applied to assess overall transcriptome phase coherence. Median absolute error of TimeSignature was higher in individual critically ill patients than healthy patients (4.90 vs 1.48 hr) and was correlated with encephalopathy severity by Glasgow Coma Scale in critically ill patients (rho, -0.54; p = 0.036). Gene expression rhythms rapidly become abnormal during critical illness. The association between disrupted transcriptome rhythms and encephalopathy suggests a path for future work to elucidate the underlying pathophysiology.

Exposure to Radiation During Work Shifts and Working at Night Act as Occupational Stressors Alter Redox and Inflammatory Markers

Studies of breast cancer etiology suggest evidence that night shift working and occupational exposure to ionizing radiation (IR) are defined risk factors for breast cancer development. There are few studies to clarify neuroendocrine and inflammatory status and the possible consequences particularly in occupational exposure. Our aim was to associate the redox and inflammatory biomarkers with either nightshift working or occupational radiation exposure, and to compare their levels between the two groups at Alexandria University Hospitals, Alexandria, Egypt. We included 150 female nurses at Alexandria University Hospitals: 50 nightshift workers, 50 radiation workers, and 50 dayshift workers as a control group (neither work nightly nor radiation workers). In morning serum sample (7 am), we measured the concentrations of serum melatonin, Cortisol, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) by ELISA; malondialdehyde (MDA) and total antioxidant capacity (TAC) levels colorimetrically, and C-reactive protein (C-RP) levels by turbidimetric method. Nightshift workers had significantly lower levels of melatonin and TAC, and higher levels of serum inflammatory markers and cortisol, than day shift control group of workers. Workers occupationally exposed to IR had significantly higher levels of serum melatonin, MDA and inflammatory markers, lower levels of serum cortisol, and lower TAC than day shift workers. Occupational exposure to IR and working nightly alter circulating redox and inflammatory biomarkers.

Влияние мелатонина на продукцию провоспалительных цитокинов при хирургии катаракты у пациентов с неэкссудативной формой ВМД

Background. In recent years, the studies concerning the role of melatonin and cytokines in the development of various pathological processes have been conducted in various fields of medicine, including ophthalmology. Purpose. To study the effect of melatonin-containing therapy on the concentration of pro-inflammatory cytokines, melatonin in the lacrimal fluid and melatonin in the blood serum in patients with non-exudative AMD in the course of surgery for uncomplicated senile cataract. Material and methods. During the study, 2 groups have been formed: group 1 (main group) – 40 patients who took the drug «Melaxen» (3 mg at night) for 3 months before the surgery; group 2 (control group) – 40 patients who did not take melatonin before the surgery. Laboratory studies of blood samples and tear fluid for the hormone melatonin and cytokines (IL-1β and IL-8) were carried out using enzyme-linked immunosorbent assay. Results. When taking exogenous melatonin in patients of the main group, the concentration of melatonin in blood serum and lacrimal fluid was significantly higher during the entire observation period, compared with the control group patients. Also, melatonin-containing therapy contributed to a decrease in the concentration of pro-inflammatory IL-8 and IL-1β cytokines. A correlation analysis of the level of cytokines and the content of melatonin in lacrimal fluid of the examined groups patients revealed a significantly higher feedback before surgery and significantly weaker and direct after the surgery. Conclusion. An increase in the concentration of melatonin in the lacrimal fluid after surgery for senile cataract in both groups indicates a possible increase in its production in response to surgical treatment and is aimed at reducing the activity of the local inflammatory process. The obtained results allow us to conclude that melatonin has an antiinflammatory effect.

The Role of Melatonin, IL-8 and IL-10 in Intrahepatic Cholestasis of Pregnancy

Intrahepatic cholestasis of pregnancy is a pregnancy-specific liver disease that usually emerges during the third trimester of pregnancy. It is characterized by itching and elevated serum total bile acid levels, and it may lead to severe fetal complications. This study aimed to investigate the role of interleukin-8, a pro-inflammatory cytokine; interleukin-10, an anti-inflammatory cytokine; and melatonin in intrahepatic cholestasis of pregnancy. This prospective, case-controlled study was conducted with 51 women with intrahepatic cholestasis of pregnancy (40 mild and 11 severe cases) and 43 healthy pregnant women. Serum interleukin-8, interleukin-10, and melatonin levels were evaluated. Melatonin and interleukin -10 were significantly lower in subjects with intrahepatic cholestasis of pregnancy (p=0.001; p=0.001, respectively p<0.05). Interleukin-8 levels were found to be significantly higher in the cholestasis group than control group (p=0.001, p<0.05). Because interleukin-8, interleukin-10, and melatonin were found to be significantly correlated with intrahepatic cholestasis of pregnancy, we believe this finding could shed light on the etiology of the disease.

Weighing and modelling factors influencing serum cortisol and melatonin concentration among workers that are exposed to various sound pressure levels using neural network algorithm: An empirical study

BackgroundNoise is one of the most common harmful agents in the workplace. Exposure to excessive noise can lead to complications such as cardiovascular disorders, disturbance of body hormones’ rhythm and hearing loss. This study aimed at weighing and modelling factors influencing serum cortisol and melatonin concentrations of workers that are exposed to various sound pressure levels using neural network algorithm. MethodologyA case-control design was adopted in the current research. The required data were collected from 75 industrial and mining firm staff members. They were assigned to three groups with equal sample sizes (25 workers). In developing the conceptual model in regard to variables that may affect workers’ serum cortisol and melatonin concentration, SPL, age, weight, and height were included. The influence of SPL on serum cortisol concentration as assessed in the three shifts. Moreover, radioimmunoassay (RIA) was utilized to assess serum cortisol and melatonin concentrations. Neural network algorithm was subsequently exploited to weigh and model predictor factors. IBM SPSS Modeler 18.0 was the software program used for data analysis. ResultsThe average cortisol concentration values for administrative, condensing, and pelletizing units respectively were 10.24 ± 2.35, 12.15 ± 3.46, and 14.91 ± 4.16μgdl. On the other hand, the average melatonin concentration values for administrative, condensing, and pelletizing units respectively were 37 ± 12.52, 34 ± 13.15, and 27 ± 9.54μgdl. According to the results of the developed model for cortisol, SPL3 (32%) and age (5%) respectively had the highest and lowest impact. On the other hand, considering the model developed for melatonin, height (27%) and SPL1 (10%) were the most and least influential factors in that order. The accuracy rates of the model were also found to be 95% for cortisol and 97% for melatonin. ConclusionComparing cortisol concentrations during various shifts revealed a significant reduction (from the beginning to the end of the shift) in all the three groups. Further, the rise of SPL would result in higher secretion of cortisol. Moreover, in all the three groups, the average serum melatonin concentration went up from the beginning to the middle of the shift and then declined to the end of the shift. Considering the accuracy rates of the models developed to predict hormones, neural network algorithm is a suitable and powerful tool for weighing and modelling factors influencing serum cortisol and melatonin concentrations.

Single melatonin treatment improves reproductive performance of Singharey goats during non-breeding season under sub-tropical condition of North East India

Abstract The effect of single subcutaneous melatonin (MEL) administration on reproductive performance in Singharey goats during the non-breeding season was studied. Forty clinically healthy female goats were selected and divided into four groups (control, MEL - 10, MEL - 20 and MEL - 40) of 10 animals each. Treatments were given as single subcutaneous injection of melatonin dissolved in one ml sterilized corn oil in different groups as MEL - 10 (10 mg/animal), MEL - 20 (20 mg/animal) and MEL - 40 (40 mg/animal). Control group was injected with placebo (one ml sterilized corn oil per animal). Oestrus induction response was 20 %, 80 %, 100 % and 30 % in control, MEL - 10, MEL - 20 and MEL - 40, respectively. Time interval to oestrus following melatonin treatment was minimum in MEL - 10 (11.25 ± 0.12 days) and maximum in control group (16 ± 5.00 days). Kidding rate was significantly (P 0.05). Serum MEL concentrations in the treated groups of goats increased significantly and the peak concentration was recorded on day 12 post treatments and thereafter it decreased sharply to reach the basal level on day 40 post treatment. The serum progesterone concentration was significantly (P

Serum Melatonin Levels in Patients with Family Mediterranean Fever

Serum Melatonin Levels in Patients with Family Mediterranean Fever

Serum Melatonin Levels in Patients with Behçet’s Disease

Serum Melatonin Levels in Patients with Behçet’s Disease

The relationship between epileptic seizure and melatonin in children

ObjectiveStudies about the relationship between epileptic seizures (ESs) and melatonin are limited in children and have been performed in heterogeneous patient groups and with different methods. In this study, it was planned to investigate this relationship according to seizure and epilepsy characteristics. Material and methodsIn 91 children with ES, serum melatonin levels were measured within half an hour following the seizure and on a seizure-free day. Seizures were categorized according to the diagnosis, semiology, etiology, duration, electroencephalography (EEG) findings, and response to treatment. Melatonin levels were compared between each group and control group. In addition, basal melatonin levels of 21 patients with electrical status epilepticus in sleep (ESES) were compared with a control group. ResultsBasal melatonin levels were found to be lower in children with ESs and ESES group compared with the control group (p < 0.001, p < 0.001). Likewise, similar results were obtained in subgroups except for remote symptomatic etiology, severe EEG findings, and refractory epilepsy. No significant difference was observed between basal and postseizure levels of melatonin. ConclusionThis is the first study to reveal the relationship between ESs and basal melatonin levels according to all the characteristics of seizure and epilepsy in the largest patient group. It also demonstrates the need for more detailed studies on the role of melatonin in the pathogenesis of both ESs and ESES, which may provide a basis for a future treatment.

Effect of diurnal intermittent fasting during Ramadan on ghrelin, leptin, melatonin, and cortisol levels among overweight and obese subjects: A prospective observational study.

Levels of cortisol, melatonin, ghrelin, and leptin are highly correlated with circadian rhythmicity. The levels of these hormones are affected by sleep, feeding, and general behaviors, and fluctuate with light and dark cycles. During the fasting month of Ramadan, a shift to nighttime eating is expected to affect circadian rhythm hormones and, subsequently, the levels of melatonin, cortisol, ghrelin, and leptin. The present study aimed to examine the effect of diurnal intermittent fasting (DIF) during Ramadan on daytime levels of ghrelin, leptin, melatonin, and cortisol hormones in a group of overweight and obese subjects, and to determine how anthropometric, dietary, and lifestyle changes during the month of Ramadan correlate with these hormonal changes. Fifty-seven overweight and obese male (40) and female (17) subjects were enrolled in this study. Anthropometric measurements, dietary intake, sleep duration, and hormonal levels of serum ghrelin, leptin, melatonin, and salivary cortisol were assessed one week before the start of Ramadan fasting and after 28 days of fasting at fixed times of the day (11:00 am-1:00 pm). At the end of Ramadan, serum levels of ghrelin, melatonin, and leptin significantly (P<0.001) decreased, while salivary cortisol did not change compared to the levels assessed in the pre-fasting state. DIF during Ramadan significantly altered serum levels of ghrelin, melatonin, and serum leptin. Further, male sex and anthropometric variables were the most impacting factors on the tested four hormones. Further studies are needed to assess DIF's impact on the circadian rhythmicity of overweight and obese fasting people.

Serum level of melatonin in patients with osteoarthritis and its relation with 8-hydroxy-2-deoxyguanosine and vitamin D

Introduction: This study aimed to evaluate the relationship between the serum level of melatonin, 8-hydroxy-2-deoxy guanosine, and vitamin-D in patients with osteoarthritis (OA). Methods: This study enrolled 47 patients with OA and 40 healthy controls. Serum levels of melatonin and 8-hydroxy-2-deoxy guanosine (8-OH-dG) were assessed. Also, serum levels of bone turnover biomarkers such as calcium, phosphorus, vitamin D, and alkaline phosphatase (ALP) were measured in OA patients and controls. Results: The serum level of melatonin was significantly lower in OA patients than the controls (6.18±2.25 vs. 11.57±3.87 pg/mL, P<0.05). In contrast, the serum level of 8-OH-dG was significantly increased in OA patients compared to controls (65.21±16.12 vs. 22.51±5.3 ng/dL, P<0.001). There was a negative correlation between serum melatonin and 8-OHdG levels in OA patients (P<0.05). There was a positive correlation between serum melatonin and vitamin D levels in OA patients (P<0.05). We found decreased calcium and vitamin D levels, and increased phosphorus and ALP levels in OA patients compared to controls (P<0.05). Conclusion: Decreased levels of melatonin and elevated levels of 8-OH-dG might play a role in the pathogenesis of OA. Therefore, melatonin might be involved in decreasing DNA damage and exerting a preventive function in OA.

Meta-analysis of melatonin levels in cluster headache-Review of clinical implications.

Cluster headache (CH) has been associated with circadian disturbances. The present systematic review examined available evidence for the utilization of melatonin (MT) in CH prophylaxis. First, case-control studies assessing nocturnal MT or its urine-expelled metabolite 6-sulfatoxymelatonin (aMT6s) in CH individuals and healthy controls (HC) were reviewed and meta-analyzed. Secondly, the results from randomized controlled trials (RCTs) and non-randomized studies evaluating MT's use in the prevention of CH were discussed. Literature search included MEDLINE, EMBASE, CENTRAL, PsycINFO, trial registries, Google Scholar, and OpenGrey. Bouts and remissions were assessed separately. Ten case-control studies (adult participants) were retrieved. Seven evaluated serum MT; meta-analysis involved only three of them (due to deficient reporting, n: bout=31, remission=38, HC=31). Results were compatible with lower nocturnal serum MT levels during bouts [bout-HC; FE-MD=-29.89pg/mL, 95% CI=(-46.00, -13.78), remission-HC; FE-MD=-2.40pg/mL, 95% CI=(-16.57, 21.38), bout-remission; RE-MD=-32.10pg/mL, 95% CI=(-56.78, -7.42)]. Nocturnal urinary melatonin was appraised in two studies, but reporting issues impeded the capitalization of the results. Nocturnal urine aMT6s was evaluated by two studies (n: bout=29, remission=22, HC=20), and pooled results were indicative of lower aMT6s concentration in CH individuals during both active and inactive periods [bout-HC; FE-MD=-9.63μg/nocturnal urine collection, 95% CI=(-14.40, -4.85), remission-HC; FE-MD=-9.12μg/nocturnal urine collection, 95% CI=(-14.63,-3.62), bout-remission; FE-MD=-0.58μg/nocturnal urine collection, 95% CI=(-4.58, 3.42)]. Regarding CH prophylaxis, one RCT and two non-randomized trials were retrieved, involving a total of 41 adult CH individuals (11-episodic, 31-chronic) and rendering the deduction of any conclusions precarious. Overall, available data for the role use of MT in CH are insufficient and inconclusive. Complementary studies evaluating endogenous MT concentrations and MT administration to patients with CH are warranted.

Melatonin down-regulates steroidal hormones, thymocyte apoptosis and inflammatory cytokines in middle-aged T. cruzi infected rats

Chagas disease, triggered by the flagellate protozoan Trypanosoma cruzi (T. cruzi) plays a potentially threat to historically non-endemic areas. Considerable evidence established that the immuno-endocrine balance could deeply influence the experimental T. cruzi progression inside the host's body. A high-resolution multiple reaction monitoring approach (MRMHR) was used to study the influence of melatonin on adrenal and plasma steroidal hormones profile of T. cruzi infected Wistar rats. Young (5 weeks) and middle-aged (18 months) male Wistar rats received melatonin (5 mg/Kg, orally) during the acute Chagas disease. Corticosterone, 11-dehydrocorticosterone (11-DHC), cortisol, cortisone, aldosterone, progesterone and melatonin concentration were evaluated. Interleukin-1 alpha and β (IL-1α and β), IL-6 and transforming growth factor beta (TGF-β) were also analyzed. Our results revealed an increased production of corticosterone, cortisone, cortisol and aldosterone in middle-aged control animals, thus confirming the aging effects on the steroidal hormone profile. Serum melatonin levels were reduced with age and predominantly higher in young and middle-aged infected rats. Melatonin treatment reduced the corticosterone, 11-DHC, cortisol, cortisone, aldosterone and progesterone in response to T. cruzi infection. Decreased IL-1 α and β concentrations were also found in melatonin treated middle-aged infected animals. Melatonin treated middle-aged control rats displayed reduced concentrations of TGF-β. Melatonin levels were significantly higher in all middle-aged rats treated animals. Reduced percentages of early and late thymocyte apoptosis was found for young and middle-aged melatonin supplemented rats. Finally, our results show a link between the therapeutic and biological effects of melatonin controlling steroidal hormones pathways as well as inflammatory mediators.

Effects of melatonin administration to cashmere goats on cashmere production and hair follicle characteristics in two consecutive cashmere growth cycles

The objective of the study was to determine the long-term effects of melatonin treatment on cashmere production and hair follicle populations in cashmere goats and their activity in two consecutive cashmere growth cycles. Twenty-four female Inner Mongolian Cashmere goats were randomly allocated to two groups (n = 12), one of which received melatonin implants, the other being an untreated control group. Melatonin implants were subcutaneously inserted behind the ear at a dose of 2 mg/kg live weight on two occasions –April 30 and June 30, 2016. Hair samples were collected by combing in April of 2017 and 2018, and the weight, staple length, and diameter of the cashmere fibers were measured. Blood and skin samples were collected monthly between April and September 2016, and in April and September in 2017 for the analysis of melatonin concentration and the characteristics of secondary hair follicle populations, respectively. The results indicated that serum melatonin concentration in the treated goats was elevated (P < 0.05) relative to that of the control group, but only during the first growth cycle. Melatonin treatment of cashmere goats in one cashmere growth cycle increased the weight, staple length, and density (all P < 0.05) of the cashmere fibers and decreased fiber diameter (P < 0.01), but did not affect the characteristics of cashmere production in the subsequent annual cycle. Melatonin treatment had no effect on the population of skin secondary hair follicles for two consecutive cycles. However, in the first growth cycle after treatment, it advanced the onset of activity of skin secondary hair follicles by 2 mo (P < 0.05), and it increased the number of follicles that were active (P < 0.05). In summary, the melatonin treatment of cashmere goats in one cashmere growth cycle improved cashmere production for that cycle only, with no residual effects on the subsequent cycle, a technique acceptable to the cashmere goat industry. The enhancement of cashmere production after the treatment of goats with melatonin appears to involve the acceleration of the annual regeneration of skin secondary hair follicles and increased population of active secondary hair follicles in the skin of cashmere goats.

Elevated Melatonin Levels Found in Young Myopic Adults Are Not Attributable to a Shift in Circadian Phase

PurposeTo evaluate the relationship between refractive error, circadian phase, and melatonin with consideration of prior light exposure, physical activity, and sleep.MethodsHealthy young myopic (spherical equivalent refraction [SER] ≤−0.50DS) and emmetropic adults underwent noncycloplegic autorefraction and axial length (AL) measures. Objective measurements of light exposure, physical activity, and sleep were captured across 7 days by wrist-worn Actiwatch-2 devices. Questionnaires assessed sleep quality and chronotype. Hourly evening saliva sampling during a dim-light melatonin onset (DLMO) protocol evaluated circadian phase, and both morning serum and saliva samples were collected. Liquid chromatography/mass spectrometry quantified melatonin.ResultsSubjects (n = 51) were aged 21.4 (interquartile range, 20.1−24.0) years. Melatonin was significantly higher in the myopic group at every evening time point and with both morning serum and saliva sampling (P ≤ 0.001 for all). DLMO-derived circadian phase did not differ between groups (P = 0.98). Multiple linear regression analysis demonstrated significant associations between serum melatonin and SER (B = –.34, β = –.42, P = 0.001), moderate activity (B = .009, β = .32, P = 0.01), and mesopic illumination (B = –.007, β = –.29, P = 0.02), F(3, 46) = 7.23, P < 0.001, R2 = 0.32, R2adjusted = .28. Myopes spent significantly more time exposed to “indoor” photopic illumination (3 to ≤1000 lux; P = 0.05), but “indoor” photopic illumination was not associated with SER, AL, or melatonin, and neither sleep, physical activity, nor any other light exposure metric differed significantly between groups (P > 0.05 for all).ConclusionsWhile circadian phase is aligned in adult myopes and emmetropes, myopia is associated with both elevated serum and salivary melatonin levels. Prospective studies are required to ascertain whether elevated melatonin levels occur before, during, or after myopia development.

IMPACT OF SMART PHONE USAGE ON SLEEP, MELATONIN AND ITS CORRELATION WITH ANXIETY AND DEPRRESSION IN FIRST YEAR MEDICAL STUDENTS

Significant increase in the smart phone use had been associated with various effect on the health. Various theories have proposed for sleep, circadian rhythm disturbances and studies have shown effect on melatonin levels and mental health. The objective of this study was to evaluate the effect of smart phone on sleep time, sleep duration and serum melatonin levels. To determine academic and non-academic usage and its correlation with academic performance. Effect of smart phone usage on the mental health i.e. anxiety and depression in young adult first year medical students. Sample size was 100 , 3 were absent. A brief questionaries’ was asked to all the study group regarding usage of mobile phone and sleep hours in night. 9 A.M Morning fasting blood samples were taken and serum melatonin was estimated by ELISA kit. Hamilton’s anxiety scale and Hamilton’s depression scale was applied to the study group. Study had shown the smart phone usage was having an impact on sleep. Study have shown late bedtime, long sleep duration in the students with high user group. This may be result of sleep displacement and circadian rhythm disturbances. Low levels of melatonin in high users as compared to low users, which may be due to exposure EMF leading to circadian rhythm disturbance. The academic and the non- academic usage was significantly high in high users, but with no significant difference in the academic performance. High academic usage of smart phone may lead to better academic performance despite of late bedtime. Non-academic usage of smart phone may cause emotional arousal leading to anxiety. Anxiety and depression score was high in high users group, but the difference was not statistically significant. But it is not clearly understood whether the smart usage is responsible for anxiety and depression or anxiety and depression causes high smart phone usage. Smartphones affect the academic lives of the undergraduate medical students. This study can conclude with the fact that using smartphones made an impact on students both positively and negatively. KEYWORDS: - Smart phone, sleep, Melatonin, Anxiety, Depression

Serum Melatonin with Respect to Mental Health (Anxiety &amp; Depression) Status of First Year M.B.B.S Students

Introduction: Melatonin is a pineal hormone secreted in circadian manner, with a peak during evening and night. Night-time light exposure is a powerful suppressor of nocturnal melatonin secretion. Dopamine and serotonin have complex relationship in melatonin synthesis and secretion. Reduced levels of melatonin have been associated with severe depression. Melatonin exhibits GABA-like effects may be responsible to reduce anxiety.&#x0D; Objectives: To determine the Serum Melatonin levels with respect to bedtime and its correlation with the severity of anxiety and depression. Also, to find the difference in anxiety score, depression score and serum melatonin level with respect to gender.&#x0D; Methods: A cross sectional study was done amongst first year medical students, and anxiety and depression score was calculated using Hamilton’s anxiety and depression scale. Estimation of Serum Melatonin was done on the fasting morning blood samples.&#x0D; Results and Conclusion: The present study shows that there is no significant difference in melatonin secretion in the students with late bedtime. With the increasing severity of the anxiety the serum melatonin was found to be lower, but the difference was not statistically significant. Similar results were observed with depression. The results shows non-significant higher anxiety and depression scores in females as compared to males. The study results also shows the significant high levels of melatonin in males as compared to females. Low melatonin levels in females may be attributed to high anxiety and depression in females.

Melatonin receptor 1A gene polymorphism rs13140012 and serum melatonin in atherosclerotic versus non-atherosclerotic Egyptian ESRD patients: pilot study

AimTo study the relationship between melatonin levels and Melatonin membrane receptor 1A (MTNR1A) SNP (rs13140012) in end-stage renal disease patients (ESRD) in Alexandria, Egypt on maintenance hemodialysis with or without atherosclerosis. Materials and methods40 end-stage renal disease patients on regular hemodialysis were divided into 2 subgroups, one with (n = 20) and one without atherosclerosis (n = 20) and normal subjects (n = 40). Serum melatonin, carotid intimal medial thickness (CIMT) were measured. Melatonin membrane receptor 1A (MTNR1A) SNP (rs13140012) genotyping was done using 5'nuclease Allelic discrimination. ResultsSerum melatonin was significantly lower in ESRD patients [1.6 to 11.30 (pg/mL) with a median of 2.5] than the control group [20.50 to 56.40 (pg/mL) with a median of 35.20]. Serum melatonin was significantly lower in atherosclerotic patients subgroup [1.6–2.50 (pg/mL) with a median value of 2.30] than non-atherosclerotic patients subgroup [2.0–11.30 (pg/mL) with a median of 4.9]. No significant association was found between serum melatonin and (MTNR1A) SNP (rs13140012) (p = 0.633). ConclusionThese results lead us to suggest that melatonin production is impaired in ESRD patients (included in this pilot study), and this impairment is more evident in atherosclerotic ESRD patients.

Serum Melatonin Levels in Patients with Sudden Sensorineural Hearing Loss

Serum Melatonin Levels in Patients with Sudden Sensorineural Hearing Loss

Changes of Serum Melatonin, Interleukin-6, Homocysteine, and Complement C3 and C4 Levels in Patients With Depression

ObjectivesCytokine activation and low complement levels are common in depression patients. This study is aimed at investigating the clinical significance of changes in serum concentrations of melatonin (MT), interleukin-6 (IL-6), homocysteine (hcy), and complement C3 and C4 in depression patients and relationships of them with depression activity.MethodsA total of 95 depression patients, including first-episode group (n = 43) and recurrent group (n = 52), and 45 age- and gender-matched healthy controls (HC) were recruited. Serum levels of MT, IL-6, hcy, C3, and C4 in all samples were measured by using enzyme-linked immunosorbent assay (ELISA), chemiluminescence method, enzyme circulation method, and immuno-scatter turbidimetric assay, respectively.ResultsThe serum MT, IL-6, and hcy levels in the first-episode group (113.08 ± 5.06 pg/ml, 2.06 ± 0.12 ng/L, and 13.87 ± 0.45 μmol/L), and recurrent group (117.63 ± 4.63 pg/ml, 2.20 ± 0.12 ng/L, and 13.61 ± 0.46 μmol/L) were significantly higher than those in the control group (89.50 ± 5.10 pg/ml, 1.57 ± 0.06 ng/L, and 11.34 ± 0.40 μmol/L). The serum levels of C3 in the first-episode group (0.95 ± 0.02 ng/L) were significantly lower than those in the recurrent group (1.05 ± 0.03 ng/L) and control group (1.12 ± 0.03 ng/L). There was no significant difference in serum C4 level between each group.ConclusionThese results suggest that higher serum MT, IL-6, and hcy levels were correlated with pathogenesis of depression.