Serum Homocysteine Levels Research Articles

Oxidative stress and one carbon metabolism in prostate neoplasm

Carcinoma of the prostate is the most common malignant tumour in men over the age of 65 years with peak age of incidence between 70 and 74 years. Oxidative stress and one carbon metabolism are important contributary factors in etiopathogenesis in Prostate Neoplasm. Significant increase in oxidative stress and serum homocysteine level and no difference of serum vitamin B12 was found in BPH and prostate cancer cases compared to control group. Following review compare level of Oxidative Stress Marker and One Carbon Metabolism markers between Prostate neoplasm patients and control Group. Antioxidant drugs may play a role in influencing outcome in these patients. However, randomized trials are required to investigate.

Personalized Biomarkers and Neuropsychological Status Can Predict Post-Stroke Fatigue

Post-stroke fatigue (PSF) is a common complication of stroke that has a negative impact on prognosis and recovery. We aimed to investigate the relationship between PSF and demographics, mood disorders, sleep disorders, and other clinical characteristics of patients with stroke. In this exploratory cross-sectional study, we collected data on sociodemographic characteristics, biological indicators, and imaging features and evaluated patients using neuropsychological scales. Patients were assessed using the Fatigue Severity Scale, Hamilton Depression Rating Scale, Hamilton Anxiety Scale, and Pittsburgh Sleep Quality Index. Magnetic resonance imaging scans were primarily used to evaluate infarctions and white matter lesions. The correlation between the PSF of patients with stroke and clinical indicators was obtained by logistic regression analysis and power analysis. We observed an independent association between fatigue severity and female sex (odds ratio [OR], 2.12; 95% confidence interval [CI], 1.14-3.94), depressive state (OR, 1.50; 95% CI, 1.01-1.73), and sleep disorders (OR, 1.58; 95% CI, 1.01-1.98). High levels of blood glucose, serum uric acid, and homocysteine and low levels of serum triiodothyronine were strongly associated with poor functional outcomes in patients with stroke. Further studies are needed to elucidate how specific structural lesions and anxiety symptoms are related to early PSF.

A Hospital-Based Case-Control Study on Raised Homocysteine Level in Vitiligo Patients and Its Association With Disease Severity.

Introduction Vitiligo is an acquired pigmentary disorderclinically manifested by circumscribed depigmented macules and often associated with leucotrichia. Not much is known about the biochemical abnormality occurring in vitiligo. Our study aims to determine whether serum homocysteine is raised in vitiligo patients and whether it can be used as a prognostic marker for vitiligo. Material and methods This study is a hospital-based, case-control, analytical study conducted on 70 patients of vitiligo patients. A total of 30 staff of the hospital served as control. Venous blood was withdrawn from the antecubital vein from all study participants using all aseptic precautions. Investigation of blood homocysteine levels was done in all the study participants. Scoring of vitiligo was done based on Vitiligo European Task Force (VETF) criteria which takeinto account body surface area, stage, and spread. Results Mean serum homocysteine level among vitiligo patients was 14.40± 5.80 micromoles/lit as compared to 10.33± 5.05 micromole/lit in control groups, and this difference was statistically significant (t-value = 3.19and p-value = 0.002). The correlation coefficient was statistically significant (correlation coefficient = 0.25 and p-value = 0.03) in between homocysteine level and stage of the disease. On multiple comparisons difference in serum homocysteine level of progressing category is significantlyraised as compared to control, stable, and regressing categories. Conclusion The mean serum homocysteine level among all vitiligo patients was higher as compared to control groups. Moreover, the serum homocysteine level of active cases is significantly higher as compared to control, stable, and regressing categories. Also, serum homocysteine levels showed a positive correlation with the degree of depigmentation, i.e., stage of the disease.

A Comparative Study to Assess the Role of Serum Homocysteine Level in Hypertensive Disorder of Pregnancy

A Comparative Study to Assess the Role of Serum Homocysteine Level in Hypertensive Disorder of Pregnancy

MTHFR C677T polymorphism, homocysteine, burden, and location of AMI and ACI.

We aimed to investigate the relationship between homocysteine levels and MTHFR C677T polymorphisms and acute ischemic vascular events and focused on the differential effects of the MTHFR C677T polymorphisms on the burden and location of AMI and ACI. 102 acute cerebral infarction (ACI) and acute myocardial infarction (AMI) patients who were admitted to the First Hospital of Jilin University in northeast China as the patient group, 83 healthy people who were hospitalized during the same period served as a control group. MTHFR C677T genotypes were identified via Polymerase Chain Reaction (PCR)-Fluorescent Probe Method. Patient group had higher serum homocysteine levels (p=0.013), lower serum folic acid (p<0.001), and Vit B12 levels (p=0.004) compared to the control group. Homocysteine levels in the patient group with the TT genotypes of the MTHFR C677T polymorphisms were higher than those with the CC and CT genotypes (p<0.05). Folic acid levels in the patients with TT genotypes were lower than those with the CC genotypes (p<0.05), but not in the control group (p>0.05). There were negative and significant associations between serum homocysteine levels and serum vitamin B12 levels in the control group (r=-0.234, p=0.033), but not between serum homocysteine levels and serum folic acid levels (r=-0.103, p=0.355). Conversely, there was a negative and significant association between serum homocysteine levels and serum folic acid levels in the patients' group (r=-0.257, p=0.01), but not between serum homocysteine levels and serum vitamin B12 levels (r=-0.185, p=0.64). No statistically significant differences in MTHFR C677T genotype and C/T alleles distribution were investigated between the patient and control group (p>0.05). The MTHFR C677T polymorphism did not differentially affect the burden and location of AMI and ACI. Homocysteine played a common role in atherosclerosis-related acute ischemic vascular events. These correlations were modified by MTHFR C677T polymorphisms and influenced by folic acid levels. The MTHFR C677T polymorphisms were not directly related to acute ischemic vascular events, nor did they differentially affect the burden and location of AMI and ACI.

Association of serum homocysteine levels with intestinal flora and cognitive function in schizophrenia

Some studies have indicated that elevated homocysteine (Hcy) levels and intestinal flora may be involved in schizophrenia (SZ) cognition pathophysiology. This study was the first to investigate the association among Hcy, intestinal flora and schizophrenia cognition. Here, 140 individuals were divided into two groups: SZ patients (N = 68) and healthy controls (HCs, N = 72). Participant data on serum Hcy levels, intestinal flora and cognitive function evaluation using the MATRICS Consensus Cognitive Battery (MCCB) were collected. Clinical symptoms of patients were evaluated using the Positive and Negative Syndrome Scale. Serum Hcy levels and the incidence of hyperhomocysteinaemia were considerably increased in SZ patients compared with HCs. Hcy levels were significantly negatively associated with verbal learning index scores (r = −0.425, p < 0.001) but positively associated with Eubacterium (r = 0.32, p = 0.007), Lactobacillus (r = 0.32, p = 0.008), Corynebacterium (r = 0.26, p = 0.035), Mogibacterium (r = 0.31, p = 0.01), and Bulleidia (r = 0.31, p = 0.01) in SZ patients. Our findings suggest that serum Hcy levels are associated with cognitive function and intestinal flora in SZ patients. However, the mechanism of the interaction between Hcy and intestinal flora and its effects on cognitive function in SZ patients requires further investigation.

STUDY OF PREVALENCE OF HYPERHOMOCYSTEINEMIA IN CHRONIC KIDNEY DISEASEPATIENTS AT TERTIARY CARE HOSPITAL IN NORTHWEST MAHARASHTRA

Introduction-Chronic Kidney Disease is growing problem across all over world and India as well. Chronic kidney disease encompasses a spectrum of different pathophysiological processes associated with abnormal kidney function and a progressive decline in glomerular filtration rate(GFR).The risk for cardiovascular disease (CVD) morbidity and mortality remains high in all stages of CKD, including in patients with hemodialysis. More patients with CKD die of CVD than of the progression of kidney failure.The increase of homocysteine level, referred as hyperhomocysteinemia, is highly prevalent in CKD patients and associated with an increased risk of CVD complications. Proposed mechanisms for hyperhomocysteinemia in kidney failure is due to reduced clearance of total plasma homocysteine (tHcy) secondary to defective kidney and/or extra kidney metabolism of tHcy and deficiencies of folate, vitamin B12, and pyridoxal 5-phosphate (PLP). Aim-To study Serum Homocysteine levels in patients with Chronickidney disease and to estimate Serum Homocysteine levels in different stages of Chronic kidney disease. Materials And Methods- All the patients presenting with clinical, biochemical and ultrasound kidney evidence of chronic kidney disease in department of medicine at tertiary care centre in northern Maharashtra. Normal plasma homocysteine level was considered to bebelow 15 umol/litre. Results- A study of 54 patients with Chronic Kidney disease of both sex were taken as sample. Of the 54 patients 38 were males and 16 were females .36 patients were in age range of 41-60 . 32 patients were in stage 5 CKD , 14 were in stage 4 CKD , 8 were in stage 3 CKD. Hyperhomocysteinemia was observed in 78 % of CKD patients. Serum Homocysteinemia levels were maximum in patients with higher grade of CKD. The associationbetween decreased GFR and increase in serum homocysteine levels was found to be statistically significant. (p&lt;0.05). Conclusion-Study concludes that hyperhomocysteinemia is highly prevalent in CKD patients. Homocysteine is commonly considered as toxicthrombogenic amino acid which causes atherosclerosis and cardiovascular diseases. Mortality due to cardiovascular diseases is high in CKD. Hence early screening and treatment for Hyperhomocysteinemia should be done in CKD to prevent cardiovascular diseases.

Homocysteine Levels in Severe OSA Patients Before and After TORS-OSA Surgery.

The increased risk of cardiovascular diseases owing to a high level of serum homocysteine has been widely reported. Literature has demonstrated that patients with obstructive sleep apnea/hypopnea syndrome (OSA) had a higher homocysteine level than control group. This study aimed to investigate the alteration of serum homocysteine levels in severe OSA patients receiving transoral robotic surgery (TORS). Retrospective chart review. Tertiary academic medical center. Data of polysomnography (PSG) and serum homocysteine levels before and at least 3 months after the surgery were collected and analyzed via paired t tests. A subgroup analysis based on the preoperative homocysteine level (≥15 mcmol/L, as hyperhomocysteinemia group) was conducted to compare the intergroup differences of homocysteine decrease. Pearson's correlation was used to survey the relationships between the changes of major PSG parameters and the levels of homocysteine decrease at baseline and after TORS-OSA surgery. Two hundred sixty-onepatients with severe OSA were enrolled. There were significant improvements in major PSG parameters after TORS-OSA surgery. Homocysteine levels significantly decreased from 12.1 ± 3.9to 11.4 ± 3.7 mcmol/L (difference = -0.7 ± 2.8 mcmol/L, p = .001) postoperatively, which was shown in the hyperhomocysteinemia group (difference = -2.9 ± 4.7 mcmol/L, p = .007) to a greater extent. Pearson's correlation revealed that ΔODI (oxygen desaturation index/h) was the predominant estimate with a positive association with Δhomocysteine (r = 0.525, p = .012). TORS-OSA surgery could decrease homocysteine levels in OSA patients. The effects were more relevant in severe OSA patients with abnormal preoperative homocysteine levels.

The relationship between serum homocysteine levels and sudden sensorineural hearing loss: a meta-analysis.

There was disagreement over the association between serum/plasma homocysteine (HCY) levels and sudden sensorineural hearing loss (SSNHL). Through the use of a meta-analysis, this study aims to determine whether there is a significant difference in serum homocysteine levels between the SSNHL group and the control group. The Cochrane Library, EMBASE, and PubMed databases were all thoroughly searched. The two independent reviewers thoroughly examined the initially searched articles. The data results were calculated by standard mean difference (SMD) or odds ratios (OR). Review Manager (version 5.3) was applied to statistical data. There were 766 participants in the 6 trials with continuous outcomes that were part of the meta-analysis A. In addition, meta-analysis B, which included 961 people, contained a total of 3 studies with dichotomous results. Both meta-analyses revealed the same conclusion that serum/plasma HCY levels in the SSNHL patients are higher than those in the controls (SMD 0.41, 95 % confidence interval (CI) 0.11 to 0.72, P < 0.01; OR 3.27, 95 % CI 2.16 to 4.94, P < 0.01). This study demonstrated that the SSNHL patients' serum/plasma HCY levels were greater than those of the control group.

Biological Role of Folic Acid in Pregnancy and Possible Therapeutic Application for the Prevention of Preeclampsia.

The rationale and importance of folic acid supplementation during pregnancy for fetal congenital defect prevention are accepted worldwide. Moreover, a sufficient plasma concentration of folates can reduce the incidence of spontaneous abortions, and support the normal expansion of placental blood vessels, ensuring physiological placental blood flow, thus promoting appropriate fetal growth and development. Furthermore, there is emerging evidence that long-term supplementation with folic acid can effectively prevent preeclampsia. Preeclampsia is unique to the human species in complications during pregnancy, which contributes to maternal and perinatal mortality worldwide. In the pathogenesis of preeclampsia abnormal placental invasion, the excess of antiangiogenic factors and maternal-placental syndrome play a key role. Increased blood levels of homocysteine during pregnancy are associated with the risk of preeclampsia. Moreover, hyperhomocysteinemia has been proposed to be an independent risk factor for preeclampsia. Folate supplementation helps to decrease elevated levels of homocysteine; thus, the role of folic acid supplementation in pregnancy is even more important. Multiple reports suggest that folate administration decreases the level of serum homocysteine and, therefore, reduce the risk and severity of preeclampsia. However, the association between folic acid supplementation and the decreased risk of preeclampsia has been investigated with controversial conclusions. Currently, the optimal dose of folic acid that is effective for preeclampsia prevention remains uncertain. In this review, we aim to summarize the accumulated knowledge on the role of folic acid in the pathogenesis of preeclampsia, and the possible impact of folate supplementation on the decreased risk of preeclampsia.

Comparison of serum homocysteine level in young and middle-aged adult patients with coronary artery disease

Deaths due to coronary artery disease have been increasing in young people aged &lt;40. Studies have reported an association between these deaths with hyperhomocysteinemia. The aim of this study was to compare serum homocysteine levels in middle-aged patients with coronary artery disease. This cross-sectional study was conducted among 52 cardiology inpatients of Bangabandhu Sheikh Mujib Medical University, Dhaka, from March 2021 to February 2022. Among them, 26 were young adults (&lt;40 years, Group I), and 26 were middle-aged (≥40 years, Group II). Serum homocysteine was measured in all of them using an automated immunoassay analyzer. The younger patients had higher (P=0.001) median homocysteine (interquartile range) level, 13.5 (7.7 –28.4 mmol/L) compared to the middle-aged group, 10.0 (5.9 – 38.5 mmol/L). The hyperhomocysteinemia (&gt;15 mmol/L) was 16.2% in Group I compared to 12.4% in Group II (P=0.001). Therefore, control measures for keeping homocysteine levels within the normal range in young people might be useful. BSMMU J 2022; 15(3): 197-200

EPIDEMIC OF CAD IN YOUNG INDIANS : THE ACCELERATED ATHEROSCLEROTIC INSULIN RESISTANCE SYNDROME (AAIRS)

Introduction: CAD is the leading cause of mortality in India. It affects Indians atleast a decade early compared to the western population. Other than the conventional risk factors, the prevalence of other risk factors like hyperhomocystenemia, lipoprotein(a), metabolic syndrome, insulin resistance and fatty liver was studied in a large registry of documented CAD in patients. To study the prevalence of insulin resi Objectives: stance syndrome and its association with NAFLD, lipoprotein (a) and homocysteine levels in young Indian patients who present with coronary artery disease It is a single centre prospective sub study of the PCAD . Materials and Methods: (Premature coronary artery disease) registry cohort at Jayadeva Institute. : 344 patients and Results 300 controls were studied. The mean age was 32 years. There were 45 females and 299 males in cases, 99 females and 201 males in control group. Smoking was seen in 138 cases compared to 16 controls which was disproportionately high in the cases group. 8% of cases had diabetes whereas only 1.6% of controls had diabetes. 10% of cases had positive family history of CAD compared to 0 in control group. 68% of cases had metabolic syndrome whereas 36% of controls had metabolic syndrome. Elevated serum homocysteine levels were seen in 49 when compared to 46 controls which was statistically signicant. (p &lt;0.001). Homocysteine level more than 15 was seen in 68% of cases compared to 45% in controls which was statistically signicant.Serum lipoprotein a levels was signicantly higher in cases (mean of 52) compared to controls (mean of 26). Lp(a) level more than 30 was seen in 51% of cases compared to 27% of controls. HOMA IR was signicantly high in the cases group when compared to controls which was statistically signicant. Fatty liver grade 0 was seen in 25% of cases compared to 58% in controls. Fatty liver grade 1 was seen in 59% of cases compared to 37% in controls. Fatty liver grade 2 was seen in 15% of cases compared to 3.7% in controls, all were statistically signicant. Novel syndrome of AAIRS Conclusion: incorporating insulin resistance, NAFLD, Lipoprotein a and homocysteine was found to be statistically different between cases and controls. Hence, this can be used to predict the risk of premature coronary disease in young Indians To Aims: formulate a novel clinical syndrome titled Accelerated Atherosclerosis Insulin Resistance Syndrome (AAIRS) which will help to predict the risk of premature coronary heart disease in young Indians To study the prevalence of i Objectives: nsulin resistance syndrome and its association with NAFLD, lipoprotein (a) and homocysteine levels in young Indian patients who present with coronary artery disease

Is Maternal Serum Homocysteine a Novel Diagnostic Biomarker for Predicting Placenta-Mediated Disorders?

Background Uteroplacental insufficiency and related disorders, though a significant cause of undesirable maternal and fetal outcomes, are complex and poorly understood. The newer screening modalities are expensive and difficult to procure for day-to-day use in developing countries. This study aimed to examine the association of mid-trimester maternal serum homocysteine levels with maternal and neonatal outcomes. Methodology This was a prospective cohort study involving 100 participants between 18 and 28 weeks of gestation. The study was conducted at a tertiary care center in south India from July2019 to September 2020. Maternal blood samples wereanalyzed for serum homocysteine levels and correlated with the third-trimester pregnancy outcomes. Statistical analysis was done, and diagnostic measures were computed. Results The mean age was found to be 26.8 ± 4.8 years. Of the participants, 15% (n = 15) were diagnosed with hypertensive disorders during pregnancy, while 7% (n = 7) had fetal growth restriction (FGR) and 7% (n = 7) were complicated by preterm birth. An elevated maternal serum homocysteine level was positively associated with adverse pregnancy outcome measures such as hypertensive disorders (p = 0.001), with sensitivity and specificity of 27% and 99%, respectively, and FGR (p = 0.03) with sensitivity and specificity of 28.6% and 98.6%, respectively. Moreover, a statistically significant outcome was noted with preterm birth before 37 weeks (p = 0.001) and a low Apgar score (p = 0.02). No association was established with spontaneous preterm labor (p = 1.00), neonatal birth weight (p = 0.42), and special care unit admission (p = 1.00). Conclusions Such a simple and affordable investigation has the potential to go a long way in the early diagnosis and management of placenta-mediated disorders in pregnancy during the antenatal period, especially in low-resource settings.

Supplementation of Water-Soluble Vitamins Reduces Hyperhomocysteinemia, Insulin Resistance, and High-Sensitivity C-reactive Protein in Prehypertension Subjects.

Prehypertensives are at higher risk of developing cardiovascular diseases. Hyperhomocysteinemia, insulin resistance, and increased high-sensitivity C-reactive protein (hs-CRP) are independent risk factors for the development of cardiovascular complications. In prehypertensives, specific therapeutic approaches can be implemented at the earliest to prevent the onset of overt hypertension. So the present study was performedto study the effect of supplementation of water-soluble vitamins on cardiovascular risk factors like homocysteine, insulin resistance, and C-reactive protein in prehypertensive subjects. Sixty prehypertensive subjects were recruited into the study based on inclusion and exclusion criteria and randomized into two groups of 30 each. One group was given a placebo and the other was given water-soluble vitamins for four months. Serum homocysteine, insulin, homeostatic model assessment of insulin resistance (HOMA-IR), and hs-CRP were assayed. After four months of treatment with water-soluble vitamins, there was a significant decrease in levels of serum homocysteine, hs-CRP, and HOMA-IR when compared to placebo treatment. After four months of treatment, there was a significant decrease in the levels of hs-CRP, homocysteine, and HOMA-IR in groups treated with water-soluble vitamins compared to the basal levels. In subjects with prehypertension, supplementation of water-soluble vitamins decreases the level of homocysteine, insulin resistance, and hs-CRP.

Using hyperhomocysteinemia and body composition to predict the risk of non-alcoholic fatty liver disease in healthcare workers.

This study investigated associations between serum homocysteine levels, body composition, and the probability of having nonalcoholic fatty liver disease (NAFLD) in Chinese healthcare workers. A total of 4028 healthcare workers were enrolled in this study, and all underwent a physical examination. Body composition was measured using multifrequency bioelectrical impedance analysis. There were 1507 NAFLD patients (72.26% male, 27.74% female) and 2521 controls (39.83% male, 60.17% female). Body mass index (BMI), waistline, neck-circumference (NC), abdominal visceral fat area (AVFA), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), glucose (Glu), homocysteinemia (hcy) were higher in the NAFLD group than controls. Additionally, the skeletal-muscle was associated with a lower risk of NAFLD, whereas BMI, waistline, NC, hyperhomocysteinemia (HHcy) were associated with a higher risk of NAFLD. The best NC cut-off point for NAFLD was 34.45cm (sensitivity 83.3% and specificity 83.9%) in women with HHcy, and the best skeletal-muscle content cut-off point for NAFLD was 41.335% (sensitivity 74.2% and specificity 65.6%) in men with HHcy. Interactions between skeletal-muscle content, NC, and HHcy may affect the incidence of NAFLD in healthcare workers. This may provide a novel approach for diagnosing NAFLD.

Homocysteine increases uterine artery blood flow resistance in women with pregnancy loss

ObjectiveUterine arterial blood flow is an important factor in embryonic development. Increased uterine artery blood flow resistance may be related to vascular damage. Homocysteine (HCY) can induce injury of endothelial through various pathways. Therefore, we investigate the association between serum HCY levels and uterine artery blood flow in the non-pregnant state in women who have experienced pregnancy loss (PL). Methods364 women eligible for PL were included in the study. The detection of HCY was completed by the Laboratory of Lanzhou University Second Hospital. We divided the patients into three groups: Low-HCY (HCY<10 umol/L, n = 144), Medium-HCY (HCY 10∼15 umol/L, n = 174) and High-HCY (HCY>15 umol/L, n = 46). The patients were subjected to vaginal color Doppler ultrasonography to measure bilateral uterine artery resistance index (RI), pulsatility index (PI) and peak systolic velocity/end diastolic velocity (S/D). ResultAmong 364 women, the right uterine artery RI in L-HCY, M-HCY, and H-HCY groups were 0.78±0.08, 0.79±0.07 and 0.81±0.07, respectively (P = 0.04). The left uterine artery RI in L-HCY, M-HCY, and H-HCY groups were 0.78±0.08, 0.81±0.07 and 0.81±0.07, respectively (P = 0.01). The right uterine artery RI level and the left uterine artery RI was significantly associated with HCY level (r = 0.103, P = 0.050; r = 0.104, P = 0.047, respectively). Of these, 177 women experienced their next pregnancy, and 33 patients experienced PL again. The pregnancy rate in l-HCY, M-HCY, and HHCY groups were 47.92% (69/144), 49.43% (86/174) and 47.83% (22/46), respectively (P = 0.95). In next pregnancy, the PL rate in l-HCY, M-HCY, and HHCY groups were 8.70% (6/69), 22.58% (22/86) and 22.73% (5/22), respectively (P = 0.03). ConclusionHCY can increase the uterine artery resistance in the non-pregnant state and is associated with the abortion rate of next pregnancy.

The relationship between hyperhomocysteinemia and total coronary artery occlusion: a cross-sectional study from Southwest China.

Increasing evidence points to hyperhomocysteinemia as an independent risk factor for coronary artery disease in addition to traditional cardiovascular risks, but few have studied the association between hyperhomocysteinemia and total coronary artery occlusion (TCAO). To understand the risk factors for TCAO, we investigated the potential relationship between hyperhomocysteinemia and TCAO, and the interactions between cardiovascular risk factors and hyperhomocysteinemia. A total of 890 adult patients from Southwest China participated in this cross-sectional study between February 2018 and February 2021. TCAO was defined as complete occlusion of more than one of the 15 coronary segments. Hyperhomocysteinemia was defined as serum homocysteine levels ≥15 μmol/L. Multivariable logistic regression models were used to determine the relationship between hyperhomocysteinemia and TCAO. The relationship between homocysteine as a continuous variable and TCAO was also analyzed. Subgroup analyses by sex, age, weight, smoking, hypertension, diabetes, and dyslipidemia were done, and interactions between subgroup variables and hyperhomocysteinemia were performed. Individuals with hyperhomocysteinemia showed an increased risk for TCAO. The adjusted odds ratio for TCAO in individuals with hyperhomocysteinemia was 1.74 (95% confidence interval, 1.28-2.36). When analyzed as a continuous variable, homocysteine was associated with an increased risk for TCAO. Subgroup analysis showed that the association between hyperhomocysteinemia and TCAO was statistically significant in men, elderly, overweight, smokers, and non-diabetic people. Interaction analysis showed no significant interactions between hyperhomocysteinemia and group variables. In Southwest China, hyperhomocysteinemia was significantly associated with TCAO. This association was particularly significant in men, elderly, overweight, smokers, and non-diabetic people.

Diagnostic Value of ATG5, Apo-Lipoprotein B-48, Thyroid Hormones, and Homocysteine in Patients with Alzheimer's Disease.

Alzheimer's disease (AD) is the most common form of dementia. In this study, serum levels of autophagy-related 5 (ATG5), apolipoprotein B-48, thyroid hormones, and homocysteine were examined in patients with AD to determine their diagnostic and predictive value for early diagnosis and prevention of AD. For this study, fifty serum samples were obtained from patients with AD and fifty serum samples from healthy controls. Serum levels of ATG 5, apo B48, thyroid hormones, homocysteine, vitamin B12, and folic acid were determined by ELISA. Spectrophotometry was used to determine serum lipid concentrations. The mean age of the case group was 69 ± 6.4 years and that of the control group was 67 ± 4.2 years. There were differences between the control and case groups in serum levels of homocysteine, apo B48, ATG5, hsCRP, LDL, HDL, cholesterol, and VitB12 (p < 0.05). According to the results of the ROC curve, measurements of serum levels of ATG5, homocysteine, and apo B48 have excellent performance in distinguishing patients with Alzheimer's disease from patients without AD. This study suggests that the measurement of serum levels of ATG5, homocysteine, and apo B48, along with other available biomarkers, can be helpful in the diagnosis and management of patients with AD in the early stages of their disease.

Association between shift work and serum homocysteine level in female electronic manufacturing services workers.

Shift work has been shown to increase the risk of cardiovascular disease (CVD) based on several evidences. The classic risk factors of CVD include age, hypertension, smoking, obesity and diabetes. Recently, the serum homocysteine level has been reported to be a valuable indicator of CVD risk. This study aimed to determine the variation in serum homocysteine level as a cardiovascular risk indicator among female workers according to shift work. The data of regular health examination of workers at an electronic manufacturing services company in Yeongnam region, South Korea in 2019 were examined in this study. The investigation was based on a cross-sectional study conducted on 697 female workers (199 day workers and 498 shift workers). The sociodemographic and biochemical characteristics were compared between day workers and shift workers. Through a logistic regression analysis, the odds ratio (OR) of the increased serum homocysteine level in relation to shift work was determined. Compared to female day workers, female shift workers showed significantly higher level of serum homocysteine (8.85 ± 2.16 vs. 9.42 ± 2.04 μmol/mL; p = 0.001). The OR of day workers against shift workers was 1.81 (95% confidence interval [CI]: 1.25-2.63). With the adjustment of variables that may influence the level of serum homocysteine, the adjusted OR was 1.68 (95% CI: 1.09-2.60). The serum homocysteine level was significantly higher in shift workers than in day workers. It is thus likely to be a useful predictor of CVD in shift workers.

The association of serum folate and homocysteine on venous thromboembolism in patients with colorectal cancer: a cross-sectional study.

Venous thromboembolism is a common complication in patients with colorectal cancer who exhibit high homocysteine and low folate levels. However, whether venous thrombosis is the result of a direct effect of folic acid or the presence of a homocysteine-mediated mediating effect cannot be determined. This study aimed to explore the association and mediating effects of serum folate and homocysteine on venous thromboembolism in patients with colorectal cancer. This study included patients with colorectal cancer who were admitted to the First Hospital of Shanxi Medical University from May 2020 to May 2022. The patients' medical records were reviewed to collect information on general demographic characteristics, the prevalence of venous thromboembolism on admission, laboratory blood indices, serum folate, and serum homocysteine. SPSS 26.0 software was used for data collation and statistical analysis; the χ2 test was utilized for univariate analysis and unconditional logistic regression was applied for multivariate analysis. R 4.1.2 was used to perform the mediating effect test. A total of 236 colorectal cancer patients were investigated. The prevalence of colorectal cancer combined with venous thromboembolism was 15.3%; serum folate was <10.75 nmol/L in 25.4% of patients; and serum homocysteine was ≥22 µmol/L in 30.5% of patients. After controlling for confounding factors, the risk of venous thromboembolism was 2.48 times greater [95% confidence interval (CI): 1.04 to 5.94] in patients with low serum folate (<10.75 nmol/L) than in those with high serum folate (≥10.75 nmol/L). Also, the risk of venous thromboembolism was greater in those with high serum homocysteine (≥22 µmol/L) [odds ratio (OR) =2.99. 95% CI: 1.11 to 8.08]. The mediating effect test showed no direct effect of serum folate on venous thromboembolism combined with colorectal cancer, and a full mediating effect of serum homocysteine between serum folate and venous thromboembolism combined with colorectal cancer, with a mediating effect value of 0.002 and a total effect value of 0.0054. Serum folate influences the formation of venous thromboembolism through serum homocysteine. It is recommended that the nutritional supplementation of patients be enhanced to control serum folate and serum homocysteine levels.