Serum Biochemical Analytes Research Articles

Haematological, Serum Biochemical and Acute Phase Protein Profiles of Sheep with Footrot Infection caused by <i>Dichelobacter nodosus</i>

Foot-related diseases are among the most common herd health issues worldwide, leading to heavy burden on the sheep and their owner including heavy socio-economic losses. This study investigated the haematological, serum biochemical and acute phase protein profiles of sheep with footrot infection caused by Dichelobacter nodosus. Blood samples from 15 sheep with footrot and 10 healthy controls were collected and analyzed for possible alterations in haematological, serum biochemical parameters and acute phase protein (APPs). Sheep with footrot showed significant changes in haematological parameters including left-shift, neutrophilic leukocytosis, lymphopenia and monocytosis. Serum biochemical assay revealed significant (P<0.05) increase in the concentration of total protein, cortisol, glucose, haptoglobin and fibrinogen while the concentration of albumin was significantly decreased. The finding of this study emphasizes the importance of haematological, serum biochemical and APP analytes in the diagnosis and monitoring of footrot in sheep. The finding of this current study could be used for the development of point-of-care diagnostic markers that would aid prompt diagnosis and treatment of footrot in sheep.

The Effects of Different Levels of Potassium Diformate and Calcium Diformate on Growth, Digestion Enzyme Activity, Antioxidant Capacity, Intestinal Flora, Stress Markers, and Some Serum Biochemical Analytes in Juvenile Beluga Huso huso

The Effects of Different Levels of Potassium Diformate and Calcium Diformate on Growth, Digestion Enzyme Activity, Antioxidant Capacity, Intestinal Flora, Stress Markers, and Some Serum Biochemical Analytes in Juvenile Beluga Huso huso

Biological variation of biochemical urine and serum analytes in healthy dogs.

Biological variation (BV) of urinary (U) biochemical analytes has not been described in absolute terms, let alone as a ratio of the U-creatinine or fractional excretion in healthy dogs. These analytes are potential diagnostic tools for different types of kidney damage and electrolyte disorders in dogs. We aimed to investigate the BV of specific gravity, osmolality, creatinine, urea, protein, glucose, chloride, sodium, potassium, calcium, and phosphate in urine from healthy pet dogs. Blood and urine samples from 13 dogs were collected once weekly for 8 weeks. Samples were analyzed in duplicate and in randomized order. For each sample, U-analyte and serum concentrations were measured, and U-analyte/U-creatinine and fractional excretion (FE) were calculated. Components of variance, estimated by restricted maximum likelihood, were used to determine within-subject variation (CVI ), between-subject variation (CVG ), and analytical variation (CVA ). Index of individuality (II) and reference change values were calculated. CVI for all urine analytes varied between 12.6% and 35.9%, except for U-sodium, U-sodium/U-Cr, and FE-sodium, which had higher CVI s (59.5%-60.7%). For U-protein, U-sodium, U-potassium, U-sodium/U-creatinine, FE-urea, FE-glucose, FE-sodium, FE-potassium, and FE-phosphate II were low, indicating that population-based RIs were appropriate. The remaining analytes had an intermediate II, suggesting that population-based RIs should be used with caution. This study presents information on the biological variation of urinary and serum biochemical analytes from healthy dogs. These data are important for an appropriate interpretation of laboratory results.

Indexing hematological and serum biochemical reference intervals of Himalayan snow trout, Schizothorax esocinus to instrument in health assessment.

Schizothorax esocinus, commonly known as snow trout, is one of the main contributors of food and livelihood in the colder zone of Himalayan region. The comprehensive information on its hematological and serum biochemical reference intervals is not reported yet. In the present study an attempt has been made to elucidate the hematological and serum biochemical reference intervals of S. esocinus from River Jhelum using protocols of the American Society of Veterinary Clinical Pathology (ASVCP). Wild fish were sampled over a period of 2years from the pollution free sites of river Jhelum. Fish blood was harvested through caudal venipuncture and hemato-biochemical analysis performed thereof. Data values from a total of healthy 432 adult fish specimens (216 male, 216 female) were systematically recorded. The reference intervals for hematological and serum biochemical parameters of S. esocinus were established using Reference Value Advisor software v 2.1. RIs for hematological and serum analytes ranged as: hemoglobin (Hb) 78.38-116.35 (g/L); white blood cells (WBC) 10-20 (×109/L); red blood cells (RBC) 1.30-2.15 (×1012/L); packed cell volume 27.00-39.45 (%); total protein 39.21-61.62 (g/L); albumin 8.20-22.02 (g/L); globulin 27.58-49.55 (g/L); glucose 3.25-7.18 (mmol/L); urea 0.96-2.38 (mmol/L); cholesterol 3.80-6.90 (mmol/L). The study also depicted that certain blood measurands were influenced with respect to sex. Significantly (p < 0.05) higher values of Hb, red blood cells count and serum glucose were noted in male as compared to female which, on the other hand, registered higher white blood cells count and serum cholesterol level (Mann Whitney U test, p < 0.05). The work, therefore, provides baseline information on hematological and serum biochemical analytes of this species which holds high commercial importance. RIs reported here can help monitor the health status of fish by improving the use of non-lethal diagnostic methods in piscine medicine.

Effect of Prolonged Serum Storage Time and Varied Temperatures on Biochemical Values in African Savanna Elephants (Loxodonta africana)

Blood samples are routinely collected from wild populations in remote locations with limited electricity, minimal diagnostic capabilities, and extreme environmental conditions. Under these conditions, serum samples may be stored for prolonged time under varied temperatures prior to processing, which could affect the ability to interpretation the results. This study’s objective was to evaluate the effects of delayed processing of serum samples and varied storage temperatures on biochemical values in African savanna elephants (Loxodonta africana). Blood samples were collected from six elephants managed by the North Carolina Zoo. For each elephant, seven red top tubes were collected. One serum sample for each elephant was analyzed on Day 0 (control group). The remaining samples were stored under different temperatures including room temperature (23 °C), refrigeration (2.2 °C), and incubation (32.2 °C), with samples from each temperature group being analyzed on Day 5 and Day 10. Many of analytes (10 out of 18) did not change significantly regardless of storage temperature or time. Refrigeration improved stability in an additional four analytes over prolonged storage. We conclude that if serum is properly separated shortly after collection, many serum biochemical analytes can be accurately measured even after suboptimal serum storage, but refrigeration and prompt evaluation are still required for some analytes.

Interrogation of an ovine serum peptide spectral library to annotate ambiguous clinicopathological biomarkers using data-independent acquisition

Background: The use of data-independent data acquisition mass spectrometry (DIA-MS) on biological samples from domestic animals is still uncommon. Here, sequential window acquisition of all theoretical mass spectra (SWATH-MS) – a variant of DIA-MS was used to analyse serum peptides of healthy sheep as compared with serum of sick sheep by interrogating a novel peptide spectral library (PSL). This approach enabled the detection and annotation of a wide range of proteins, than conventional clinical pathology protein assays. Methods: Serum samples from healthy sheep were obtained from a commercial source and normalised to represent a healthy sheep proteome background and then compared with serum samples of sheep suffering from a range of naturally-acquired illnesses submitted to The University of Queensland, Australia. Purified tryptic peptides were subjected to liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) on a quadrupole time-of-flight instrument (TripleTOF 5600+, SCIEX) set in a cyclic data-independent acquisition (DIA) mode using a generic (SWATH™, SCIEX) acquisition method. Data were processed using PeakView® v2.2 software with SWATH™ Acquisition MicroApp 2.0 (SCIEX) and MarkerView™ v1.3 software (SCIEX) pipeline to generate protein lists for downstream gene ontology annotation and pathway analysis of identified proteins. Results: There were distinct differences in peptide chromatographic features of sick sheep samples compared to those from healthy sheep. Healthy and sick sheep serum samples yielded 335 and 236 protein identifications (IDs), respectively. There were 96 protein IDs unique to sick sheep serum. A total of 431 protein IDs were annotated by combining healthy control and sick sheep protein IDs. Conclusions: SWATH analysis successfully aided in the detection some established clinicopathological serum biochemical analytes. This approach enabled the distinction of protein profiles of sick sheep samples from a healthy control sample, thereby providing a promising future perspective for the application of SWATH analysis in veterinary clinical use.

CLINICOPATHOLOGIC EVALUATION OF AN EX SITU POPULATION OF ADULT AFRICAN WHITE-BELLIED PANGOLIN (PHATAGINUS TRICUSPIS).

Death due to illegal trade and trafficking serves as the largest threat to pangolins in their geographic range countries. Appropriate veterinary care is essential to the welfare of sick or debilitated pangolins. Given the challenge of comprehensive physical examination of this species due to its unique anatomic features and poorly studied physiology, laboratory diagnostics can provide essential information to guide appropriate medical care. This retrospective study included previously analyzed blood and urine samples collected from adult (n = 17) African white-bellied pangolins (Phataginus tricuspis) under managed care that were sampled over a 4-yr period as part of routine veterinary examinations. Samples from clinically normal animals were used to summarize results for this species. Pangolins had a lymphocyte-predominant leukogram with a mean neutrophil to lymphocyte ratio of 0.83 (min-max: 0.13-4.81). Seasonal, sex, and age differences were present for several hematologic and serum biochemical analytes. Urine samples had a median urine specific gravity (USG) of 1.040 (min-max: 1.006-1.060), a neutral-to-basic urine pH (6.0-8.6), and contained mainly struvite crystals or infrequently calcium oxalate dihydrate crystals. Winter samples had higher USG than summer samples. The clinicopathologic data provided herein offers applicable information useful for the clinical management of pangolins.

Age- and sex-related changes in body weights and clinical pathology analytes in cynomolgus monkeys (Macaca Fascicularis) of Mauritius origin.

BackgroundClinical pathology and body weight information for the cynomolgus monkey in the literature is primarily derived from a small number of animals with limited age ranges, varying geographic origins, and mixed genders.ObjectivesThis study aimed to summarize the age‐ and sex‐related changes in clinical pathology analytes and body weights in cynomolgus monkeys of Mauritian origin.MethodsPre‐study age and body weight data were reviewed in 1819 animals, and pre‐study hematologic, coagulation, and serum biochemical analytes were reviewed in 1664 animals.ResultsBody weights were statistically higher (P < 0.01) in males than females in all age groups (2–10 years). These measurements became prominent after 4 years of age and peaked at 7 to 8 years of age in both sexes. Sex‐related differences were noted in reticulocyte (RETIC) counts, creatinine, cholesterol, and triglyceride concentrations, and alkaline phosphatase (ALP) and gamma‐glutamyl transferase (GGT) activities. Age‐related differences were noted in RETIC and lymphocyte counts, creatinine, triglyceride, phosphorus, and globulin concentrations, and ALP and GGT activities. The youngest (2 to <3 year) age group had the fewest number of clinical pathologic analyte differences including ALP and GGT activity differences which occurred in all age groups from 2 to 10 years; they also had age‐related lower globulin concentrations. There were no age‐ or sex‐related differences in coagulation measurands.ConclusionsSexual dimorphism in body weight was apparent for all ages from 2 to 10 years of age. The only difference in clinical pathology analytes unique to the 2 to <3 years of age group were age‐related lower globulin levels.

Seasonal variations in hematological and serum biochemical analytes of snow trout, Schizothorax esocinus inhabiting Dal Lake

Seasonal variations in hematological and serum biochemical analytes of snow trout, Schizothorax esocinus inhabiting Dal Lake

Reference intervals for hematological and serum biochemical analytes in snow trout, Schizothorax esocinus inhabiting Dal Lake of Kashmir Himalaya

Reference intervals for hematological and serum biochemical analytes in snow trout, Schizothorax esocinus inhabiting Dal Lake of Kashmir Himalaya

Effect of residual detergents in specimen collection containers on routine serum biochemical analytes

International Journal of Multidisciplinary Studies (IJMS) is published by the Faculty of Graduate Studies - University of Sri Jayewardenepura. IJMS provides a supportive environment for multidisciplinary researchers to publish their contribution to the academy of knowledge and wisdom. Focus of the journal is on multidisciplinary research which should cover at least two distinct disciplines.

The effect of age and sex on selected hematologic and serum biochemical analytes in 4,804 elite endurance-trained sled dogs participating in the Iditarod Trail Sled Dog Race pre-race examination program.

Endurance-trained sled dogs provide a unique translational model to characterize changes in hematologic and serum biochemical analytes due to the aging process. The primary objective of this study was to determine the effect of age and sex on specific hematologic and serum biochemical parameters in the endurance trained sled dog. Longitudinal and cross-sectional data were analyzed from 9,746 blood and serum samples from 4,804 dogs collected over 7 years as part of the Iditarod Trail Sled Dog Race pre-race examination program. Mixed models analysis was used for statistical analysis and P < 0.01 was considered significant. Dogs ranged from 1–12 years of age and 39% were female. Serum total calcium and phosphorus concentrations and white blood cell count decreased nonlinearly to asymptotic values by 6.6, 3.1, and 6.9 years of age, respectively, equivalent to estimated physiologic ages in human years of 44, 27, and 46 years. Serum glucose concentrations reached their lowest value at 7.8 years of age, equivalent to an estimated human physiologic age of 50 years, after which time the concentration increased. Serum globulin concentrations increased with age, but nonlinearly for females and linearly for males. Most sex-related differences were <5%; however, females had lower serum urea nitrogen (14.7%) and creatinine (7.3%) concentrations, lower serum alanine aminotransferase activity (16.6%), and higher serum total bilirubin concentration (12.8%) and platelet count (6.0%). The endurance-trained sled dog provides an excellent model to separate the physiologic effects of age from those of a sedentary lifestyle on hematologic and serum biochemical analytes.

Hematological and Biochemical Reference Intervals for 5 Adult Hunting Dog Breeds Using a Blood Donor Database.

Simple SummaryHematological and biochemical profiles are essential in the diagnosis and monitoring of disease in veterinary medicine, requiring optimal Reference Intervals (RIs) for accurate interpretation. The aim of this study is to determine hematobiochemical RIs for 5 hunting dog breeds from a blood donor database and to compare them with laboratory established and published RIs to identify possible breed and attitude-related differences. A total of 445 healthy adult hunting dogs (156 Ariégeois, A; 52 Bleu de Gascogne, B; 64 Bracco italiano, C; 123 Segugio italiano, D; 50 Briquet Griffon Vandeen, E) were included in the study. Significant differences in 12 hematologic and serum biochemical analytes, for which a breed-specific variation appears to be the most plausible explanation, were detected, and new RIs for these parameters are provided.Numerous studies have shown the importance of breed-related differences between hematological and biochemical results in veterinary medicine. The aim of this study is to determine hematologic and biochemical Reference Intervals (RIs) for 5 hunting dog breeds from a blood donor database, adopting an indirect sampling method, and to compare them with laboratory established and published RIs to identify possible breed and attitude-related differences. The study analyzed the blood parameters of 445 adults (222 females and 223 male, with age ranging from 2 to 8 years, mean age 5.3 years), client-owned, clinically healthy blood donor dogs of 5 breeds: 156 Ariégeois, 52 Bleu de Gascogne, 64 Bracco italiano, 123 Segugio italiano, and 50 Briquet Griffon Vandeen. Statistical analysis was performed as recommended by the American Society of Veterinary Clinical Pathology (ASVCP) guidelines. RIs for red blood cells (RBC), hematocrit (HCT), hemoglobin (HB), main corpuscular volume (MCV), main corpuscular hemoglobin (MCH), main corpuscular hemoglobin concentration (MCHC), red distribution widht (RDW), white blood cells (WBC), and differential leukocytes count, PLT, Albumin, Total Protein, Urea, Creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) for each of the 5 breeds were performed, and significant differences with the established RIs were detected. We found significant differences in 12 hematologic and serum biochemical analytes for which a breed-specific variation appears to be the most plausible explanation. New RIs for HCT, MCH, MCHC, RDW, PLT, Monocytes, Eosinophils, Albumin, Urea, Creatinine, AST, and ALT are provided for at least 1 breed. Breed-specific RIs for adult hunting dogs will help avoid misinterpretation of laboratory results in these breeds.

Haemogram and Serum Biochemical Values of Four Indigenous Species of Monkeys in South West Nigeria.

Haematological and serum biochemical values are useful guides and biomarkers in health and diseases for reaching a diagnosis, estimating disease prognosis and monitoring treatment progress, in mammals. Reference ranges for some parameters differ among species of mammals and between sexes within a species. There is dearth of information on standard reference value for blood parameters for Nigerian indigenous monkeys. Whole blood and serum samples obtained from 50 apparently healthy adult monkeys in both captivity and from the wild in southwest Nigeria were subjected to haematology and serum biochemistry to obtain preliminary reference values for haematological and serum biochemical analytes for Cercocebus sebaeus (Green monkey), Cercopithecus mona (Mona monkey), Erythrocebus patas (Patas monkey) and Papio anubis (Anubis baboon). Numerical data were summarized as mean and standard deviation and subjected to statistical analysis; Student t test and analysis of variance, to compare values of blood parameters obtained between species and gender. A p-value less than 0.05 was considered significant. The hematocrit of male animals were significantly higher than that of females (P=0.01) in all the 4 species studied but there was no significant difference in other blood parameters such as total white blood cell and the differential counts, platelet count, serum aspartate transferase, alanine transferase, alkaline phosphatase, total plasma protein, albumin, and globulin concentrations between the sexes. Generally, there was no significant difference between total white blood cell and the differential counts, hematocrit, red cell count, haemoglobin concentration, platelet count, serum aspartate transferase, alanine transferase, alkaline phosphatase, total plasma protein, albumin, and globulin concentrations among the monkey species.

Serum Biochemical Analytes and Trace Elements in Juvenile Whooping Cranes (Grus americana)

Biochemical and trace element analyses of blood from wild Whooping Cranes (Grus americana) were performed to assess the health of the only self-sustaining, migratory population in North America. Juvenile cranes (n=31) approximately 49-70 d-old were sampled at Wood Buffalo National Park, Northwest Territories, Canada, in midsummer from 2010 to 2012. Archived serum (n=24) and whole blood (n=31) samples from captive juvenile cranes were selected as age-matched controls. Reference values were calculated for serum biochemical analytes and trace elements in whole blood from the captive juvenile Whooping Cranes reared under controlled conditions and with known health histories. Several statistical differences among blood biochemical and trace element values of the wild and captive juveniles were identified and were likely attributable to dietary differences between the populations.

Hematologic and serum biochemical reference intervals for wild eastern quolls (Dasyurus viverrinus): Variation by age, sex, and season.

The eastern quoll (Dasyurus viverrinus) is an endangered carnivorous marsupial that has recently suffered significant population declines. Several small captive breeding populations have been established, with plans to translocate wild and captive individuals to areas of their former distribution. Accordingly, hematologic and serum biochemical reference intervals (RIs) established from wild eastern quoll populations are essential for monitoring the health and disease status of both captive and wild populations, and to evaluate the health of individuals before, during, and after translocation. We aimed to establish hematologic and serum biochemical RIs for wild eastern quolls, and examine the effects of age, sex, and season. We collected a total of 202 hematologic samples, 309 packed cell volume samples, and 335 serum biochemical samples from 168 individual quolls between May 2011 and November 2013. Species-level RIs were established, as well as RIs of groups separated by age (juvenile, adult) and sex (adult male, adult female) using nonparametric, robust, and parametric methods. Seasonal variation in age- and sex-specific reference values was also assessed. Strong age and seasonal variation were evident in many hematologic and serum biochemical analytes, with significant variation observed in serum biochemical analytes between the sexes. The observed age, sex, and seasonal variation reflect differences in the timing of growth and reproductive stressors, which interact with seasonal energetic demands. Our findings highlight the importance of using age-, sex-, and season-specific RIs for clinical evaluation of eastern quolls, as species-level RIs will inadvertently smooth and mask important seasonal fluctuations that reflect reproductive status at different times.

Recurrence of extreme serum analytes in consecutive pregnancies and association with obstetrical outcomes*

Objective: To evaluate if presence of extreme maternal serum biochemical analytes recurs in consecutive pregnancies. We hypothesized that presence of >1 extreme analyte in prior pregnancy is associated with increased risk of adverse pregnancy outcome in subsequent pregnancy. Methods: Retrospective cohort study of singleton pregnancies evaluated and delivered in 2 consecutive pregnancies (2011–2015). Adverse outcomes were defined as indicated preterm delivery before 37 completed weeks due to preeclampsia, fetal growth restriction or other complications. Results: First and second trimester maternal serum analytes were assessed in 1434 patients in 2 consecutive pregnancies. The presence of >1 extreme serum analyte in prior pregnancy significantly increased likelihood of >1 extreme analyte in subsequent pregnancy. The likelihood increased as number of prior extreme markers increased. In patients with normal outcomes and 2 or more extreme serum analytes in prior pregnancy, there was an increased incidence of adverse pregnancy outcomes in subsequent pregnancy with relative risk (RR) of 5.42 [95% CI 1.6–18.3]. Conclusions: The presence of more than 1 extreme serum marker in one pregnancy increases likelihood of recurrence in subsequent pregnancies. Risk of adverse outcomes in subsequent pregnancy can be evaluated based on biochemistry results as well as prior pregnancy outcomes.

A dramatic blood plasticity in hibernating and 14-day hindlimb unloading Daurian ground squirrels (Spermophilus dauricus).

We compared the effects of hibernation inactivity and 14-day hindlimb unloading in non-hibernating period on biochemical, rheological, and hematological parameters of blood in Daurian ground squirrels (Spermophilus dauricus). Twenty-four squirrels were randomly divided into four groups: control (CON), hibernation (HIB), post-hibernation (POST), and 14-day hindlimb unloading (HU). The results showed that serum enzymes (L-lactate dehydrogenase, alanine aminotransferase, and aspartate aminotransferase) activities decreased in HIB, POST, and HU squirrels compared with CON. Total protein (including albumin and globulin) maintained in HIB but decreased in HU compared with CON. Total cholesterol and high-density lipoprotein-cholesterol increased in HIB but maintained in HU and POST compared with CON. Meanwhile, serum creatinine decreased and urea increased in HU compared with CON. All blood ions concentrations were unchanged in HIB, POST, and HU squirrels compared with CON except calcium which increased in HIB compared with CON, and phosphorus which increased in HIB and POST compared with CON. Most of detected serum biochemical analytes in POST recovered to the CON level. Blood viscosity, which was unchanged in all shear rates in HU, increased in HIB and recovered in POST in lower shear rates compared with CON. Erythrocyte and corpuscular volume decreased in HIB and HU but maintained in POST compared with CON. All the routine hematological parameters recovered in POST as compared with CON except platelet, which decreased in HIB and POST but maintained in HU compared with CON. In conclusion, our results suggested a remarkable ability to maintain blood homeostasis in hibernating squirrels.

Neither creatinine- nor cystatin C-estimated glomerular filtration rate is optimal in oncology patients treated with targeted agents.

In the last decade, there has been an increase in the use of anti-angiogenic drugs as treatment for metastatic malignancies. However, use of these targeted therapies could induce both glomerular and tubular damage. Also during targeted therapy, the lysosomal protease cathepsin D is released from the tumour, which is inhibited by the protease inhibitor cystatin C. The aim of this study is to determine if use of cystatin C-estimated glomerular filtration rate (eGFR) is applicable to a patient cohort treated with targeted agents. A cohort of 80 patients with various malignancies were continuously recruited and prospectively analysed. Serum and urinary biochemical analytes for renal toxicities were assessed at different time points during treatment. The association between serum cystatin C and cathepsin D was also determined. A decrease in serum cystatin C concentrations (1.03 versus 0.90 mg/L; P < 0.001), together with an increase in cystatin C-eGFR (71 versus 89 mL/min/1.73 m2; P = 0.002) was observed during therapy, compared with baseline. This decrease in cystatin C concentrations was correlated with cathepsin D (r = 0.307; P < 0.001), which was released from the tumour during targeted therapy. Further analysis demonstrated cathepsin D-mediated proteolysis of cystatin C in serum. Cystatin C concentrations were decreased during targeted therapy due to cathepsin D-mediated proteolysis. Cystatin C-eGFR is therefore not considered a suitable marker for assessing kidney function in oncology patients, and other techniques to estimate the GFR have to be applied in this patient population.

Changes in biochemical analytes in calves infected by nematode parasites in field conditions

Parasitic infections caused by nematodes are a major problem in bovines that resulting in losses in animal health and production. Thus, the aim of this study was to evaluate alterations in selected serum biochemical analytes in calves naturally infected with gastrointestinal (GI) and pulmonary nematodes without clinical signs. For this, samples of feces and blood of 86 calves were collected. Fecal egg counts (FEC) were determined using the modified McMaster technique with a sensitivity of 50 eggs per gram of feces (EPG). Positive nematode FEC was processed for coproculture using pooled samples to identify Strongylidae infective larvae (L3). First stage-larvae (L1) of Dictyocaulus viviparous were identified by a modified Baermann method. The biochemical analytes determined were: acute phase proteins such as haptoglobin and paraoxonase type 1; the enzymes acetylcholinesterase; butyrylcholinesterase; the lipid profile (triglycerides and total, HDL, and LDL-cholesterol); serum iron profile (iron and unsaturated iron-binding capacity); total protein and albumin; pancreatic profile (amylase and lipase); and minerals (phosphorus and calcium). The calves were divided into four groups according to the results of EPG and the modified Baermann method. Group 1: healthy control animals (n=16); Group 2: calves with only GI parasites (n=51): This group was sub-divided into sub-groups according to the EPG threshold: 2a—GI parasites with low EPG (n=23), and 2b—GI parasites with high EPG (n=28). Group 3: animals with only lungworms (n=5), and Group 4: calves with lung+GI parasites (n=14). The more prevalent genera in all coprocultures were: Cooperia spp., Haemonchus spp., Oesophagostomum spp., and Trichostrongylus spp. The nonparametric Kruskal–Wallis test was used to compare the groups and Dunn's post-test was used for multiple comparisons as the data was not normally distributed (P<0.05). The haptoglobin concentration increased in calves with GI and pulmonary parasites. A significant increase in acetylcholinesterase was observed in calves infected with lungworms. Cholesterol, triglycerides, HDL, and LDL concentrations decreased but lipase concentration increased in calves with GI parasites. Therefore, this paper provides an overview of the biochemical effects produced by nematode parasites in calves in field conditions. These findings in calves without any evident clinical signs of disease could provide an indication of GI parasites and lungworm infection, especially in an endemic area for these parasites.