Seroprevalence Of Leptospiral Infection Research Articles

Comparison of Leptospira interrogans infection in the goats and sheep

Background: Most leptospiral infections in sheep and goat are asymptomatic but may result in high fever, abortion, stillbirth, agalactiae. There is a different foraging behavior between sheep and goat that may cause the different prevalence of Leptospira interrogans infection in sheep and goats. OBJECTIVES: The purpose of the present study was to compare the prevalence of L. interrogans antibodies in sheep and goats. METHODS: Blood samples were taken from 246 sheep and 210 goats in 12 herds from 8 areas of Ahvaz where the animals were kept together. Sera were initially screened at dilution of 1:100 against 8 live serovars of L. intrrogans: pomana, canicola, hardjo, ballom, ictrohaemorrhagiae, grippotyphosa, tarasovi and australis using the microscopic agglutination test (MAT). RESULTS: The prevalence of leptospiral infection was 8.53% in sheep and 10.95% in goats. The highest reacting leptospira in both species was L.i. Pomona with a reactor rate of 68.18% in sheep and 56% in goats, followed in descending order by ictrohaemorrhagiae (18.8%), canicula, hardjo and grippotyphosa (each of them 4.54%), in sheep and ictrohaemorrhagiae (28%), canicula (16%) in goats. Statistical analysis showed that were no significant differences between sheep and goat (P=0.428). There were no significant differences among age groups in sheep (p=0.301) and goats (p= 0.363), but there was a tendency in adults sheep and goats (≥3years) to be more seropositive than young sheep and goats. Seroprevalence of leptospiral infection among various areas in sheep (p= 0.464) and goats (p= 0.710) was also not significantly different. CONCLUSIONS: It is concluded that there is no difference between sheep and goats in terms of leptospiral infection when they are kept together in the same herd and husbandry condition.

Seroprevalence of Leptospiral infection in goats of Ahvaz

In a serological survey to investigate the prevalence of leptospiral infection in goats in Ahvaz, blood samples were taken from 172 female goats. They were initially screened at serum dilution of 1: 100 against six live antigens of leptospira interorgans serovar pomona, canicola, hardjo, ballum, icterohemorrhagiae, and grippotyphosa using the microscopic agglutination test (MAT) and samples were considered positive, if 50% or more of agglutination of leptospires in a dilution of l: 100 or greater was found. Sera with positive results were titrated against reacting antigens in serial twofold dilution from l:100 to 1:1600.Antibodies against one or more serovars were detected in 18 (10.46%) sera at dilution | 1: 100. Antibodies against more than one serovar were found in 5 (27.77%) positive sera. Antibodies were most frequent to serovar grippotyphosa (39.13%) followed by in descending, canicola (26.80%), pomona (21.72%), icterohemorrhagiae (8.69%) and hardjo (4.34%).

Seroprevalence of Leptospiral Infection in Rodents of Dairy Cattle Herds Complexes in Suburb of Mashhad—Iran

Talebkhan Garoussi, M., Vand-e-Useefee, J. and Mehrzad, J. 2006. Seroprevalence of Leptospiral infection in rodents of dairy cattle herds complexes in suburb of Mashhad-Iran. J. Appl. Anim. Res., 30: 109–111. A total of 115 rodents including 66 mole rats (Nesokia indica) and 49 house mice (Mus musculus) were trapped from 18 commercial dairy cattle herds/farms in Mashhad suburb of Khorrassan Razavee province, Iran. The microscopic agglutination test (MAT) with 22 live antigens were used to determine serological responses. The seroprevalence (at a titer ≥100) was 21.73%. Serum samples showed positive reaction against Leptospira interrogans serogroups/serovars Icterohaemorragiae icterohaemorragiae, Grippothyphosa grippothyphosa and Sejroe hardjo and the distribution of MAT titer for serogroups/serovars were 1:200, 1:400, 1:800 and 1:1600, respectively. The infection rate was significantly higher in mole rats than house mice (P<0.05). However, there were no significant differences among species and different infection titers.