Herbicides are part of modern agricultural production systems and therefore contribute significantly to the economy of agricultural products. In the United States, losses caused by weed competition with major crops is approximately $8.9 billion, while the total expenditures for herbicides used is about $2.1 billion and the cost of applying these herbicides, $938 million annually (Chandler 1985). Without current technology to control the weeds, the losses would be doubled or tripled. At the same time, herbicides are potent and specific inhibitors of plant metabolism and may therefore be potentially useful as valuable tools in basic plant physiological research. Unfortunately, few herbicides are known to inhibit plant growth by direct inhibition of enzymes involved in metabolic pathways. Thus, most literature dealing with herbicidal effects on plants describes secondary and tertiary effects (Duke 1985a) such as chlorosis. The environment has been assaulted with a variety of chemical agents, among which herbicides contribute significantly. Some of these have appeared to be relatively innocuous, while others are quite hazardous. Thus, the biodegradability of a herbicide may be a factor in the decision to use it. In this paper we describe the usefulness of a relatively novel herbicideglyphosate-for a laboratory demonstration of herbicidal activity, mode of action and stability in soil. A series of laboratory exercises conducted by students is used to demonstrate several properties of this herbicide. Glyphosate, N-(phosphonomethyl) glycine (see Figure 1), is an extremely effective, non-selective, post-emergence herbicide with an increasing number of international applications. It is the active ingredient of Roundup?, Monsanto's herbicide formulation consisting of glyphosate isopropylamine salt and surfactant solution in water. Roundup? has developed into an extremely important herbicide since its introduction in 1971 (Baird et al. 1971; Franz 1985) and now is marketed in more than 100 countries. Glyphosate has a relatively low molecular weight and high water solubility, factors which aid in its rapid absorption and translocation by plant tissues. Once inside the plant, glyphosate does not break down, nor is it metabolized to a significant degree. In soils, however, the compound is strongly absorbed (preventing leaching) and is rapidly degraded by microorganisms to to non-toxic organic products (see Figure 1) which are then broken down to ammonia, water and carbon dioxide (Hoagland & Duke 1981). Moreover, glyphosate is non-toxic to insects and vertebrates and does not accumulate in animal tissues (Newton et al. 1984; Sullivan 1985). In early studies on the mode of action of glyphosate (Jaworski 1972; Roisch & Lingens 1974), it was found that the growth inhibitory effects on both Rhizobium japonicum and duckweed (Lemna gibba) could be completely reversed by addition of the aromatic amino acids: phenylalanine, tyrosine and tryptophane. In later studies, in which profiles of free pools of aromatic amino acids from higher plants were examined, levels of aromatic amino acids were found to be greatly reduced in comparison to other amino acids (Duke 1985b). Studies by Amrhein (Steinriicken & Amrhein 1984; Duke 1985b) established that reduced levels of aromatic amino acids resulted from inhibition of a single enzyme of shikimate pathway (one of the two metabolic pathways producing aromatic compounds in plants and bacteria; this pathway does not operate in animals) namely 5-enolpyruvylshikimate-3phosphate synthetase. The molecular basis of this inhibition is now well understood (Steinrucken & Amrhein 1984).