Extraction Of Sericin Research Articles

In silico and in vitro Antityrosinase Activity of the Non-Protein Fraction from the Sericin Extract of Antheraea proylei J.

In silico and in vitro Antityrosinase Activity of the Non-Protein Fraction from the Sericin Extract of Antheraea proylei J.

Development of Biodegradable Bioplastics with Sericin and Gelatin from Silk Cocoons and Fish Waste.

The bioplastics sector promotes environmentally friendly means of cutting down on the usage of fossil fuels, plastic waste, and environmental pollution. Plastic contamination has detrimental effects on both ecological systems and the global food supply. The approach we present here to resolve this issue involves the integration of sericin and gelatin, obtained from cocoon and fish waste, respectively, with nano-reinforced cellulose crystals, to develop a biodegradable and compostable plastic material. The use of cocoon and fish wastes for the extraction of sericin and gelatin presents an environmentally beneficial approach since it contributes to waste reduction. The sericin level found in silk cocoon waste was determined to be 28.08%, and the gelatin amount in fish waste was measured to be 58.25%. The inclusion of sericin and gelatin in bioplastics was accompanied by the incorporation of glycerol, vinegar, starch, sodium hydroxide, and other coloring agents. Fourier transform infrared (FTIR) examination of bioplastics revealed the presence of functional groups that corresponded to the sericin and gelatin components. The tensile strength of the bioplastic material was measured to be 27.64 MPa/psi, while its thickness varied between 0.072 and 0.316 mm. The results of burial experiments indicated that the bioplastic material had a degradation rate of 85% after 14 days. The invention exhibits potential as a viable alternative for packaging, containment, and disposable plastic materials. The use of this sustainable approach is recommended for the extraction of sericin and gelatin from silk cocoons and fish waste, with the intention of using them as raw materials for bioplastic production.

Extraction of Sericin from Cocoons of the Silkworm Bombyx Mori, Its Characteristics, and a Dietary Supplement on Its Basis to Prevent Diabetes Mellitus

Extraction of Sericin from Cocoons of the Silkworm Bombyx Mori, Its Characteristics, and a Dietary Supplement on Its Basis to Prevent Diabetes Mellitus

Antioxidant and cholesterol regulatory effect of flavonoid-rich silk sericin.

Cholesterol is a component of cell membranes and a precursor of hormones, and excess levels are associated with disease development; therefore, it must be maintained within the normal range. Silkworm cocoons are known to contain bioactive substances. Therefore, we compared the bioactivities of pigmented and white silkworm cocoons. Sericin extract of the Yeonnokjam (YN) variety, which contained a high flavonoid content, showed the highest antioxidant activity and inhibited cholesterol biosynthetic enzyme activity. YN-fed mice showed a 26% reduction in serum low-density lipoprotein cholesterol level. In addition, a 27% decrease in cholesterol accumulation in the liver was observed. Mechanistically, YN reduced the expression of 3-hydroxy-3-methylglutaryl-CoA reductase and acetyl-CoA acetyltransferase 2 proteins by 34 and 13%, respectively. In conclusion, YN suppresses cholesterol synthesis in the liver and stimulates bile acid secretion, which contributes to reduction in cholesterol levels, suggesting its potential as a cholesterol-lowering agent.

Extraction and characterization of sericin obtained from unreelable cocoons of Bombyx mori L

Extraction and characterization of sericin obtained from unreelable cocoons of Bombyx mori L

Extraction, characterization and functionality assessment of Aloe vera, chitosan and silk sericin

Aloe vera, chitosan, and silk sericin have been investigated for their physicochemical properties as well as functional characteristics such as antibacterial, antioxidant, and UV resistance. Aloe vera was extracted by methanol as a solvent. Silk sericin was prepared from a boiled water solution of silk cocoons through ethanol precipitation, while chitosan was synthesized by deacetylation of chitin. FT-IR, TGA, DSC, EDX, UV–vis, and XRD were used to characterize the extracted Aloe vera, chitosan, and sericin. Antibacterial activity, antioxidant activity and UV protection factors were also investigated. The TGA graph revealed that the significant weight loss at 400 °C was 39%, 51%, and 52%. for Aloe vera, chitosan, and sericin, respectively. For E. coli, Aloe vera, chitosan, and silk sericin, the zones of inhibition were 2.5 mm, 3 mm, and 1 mm, respectively, whereas for S. aureus, the zones of inhibition were 4 mm, 5 mm, and 1.5 mm. The free radical scavenging activity of 10 mg/ml Aloe vera, chitosan and sericin was 93%, 53% and 52%, respectively. All three UV radiation zones were absorbed by an Aloe vera solution. Chitosan and silk sericin solution exclusively absorbed UV-C rays. These almost priceless natural resources can contribute to health-related beneficial issues.

Extraction and Characterization of Sericin from Cocoon of Four Different Silkworm Races Bombyx Mori L.

The removal and use of sericin could have a strong economic, social, and environmental impact, particularly in countries where sericulture is practiced, especially in Bangladesh. Because in our country Bangladesh, sericin is a waste material in the sericulture industry. Several techniques have been adopted for sericin extraction, but maintaining its chemical properties after extraction and environment-friendly extraction methods are still a major challenge. Sericin is fully or partially hydrolyzed and solubilized during the degumming of the cocoon. Consequently, it is important to create sericin extraction procedures that require less energy, don't release any chemicals, and don't harm the environment. Numerous research has been done to extract sericin but the differences in sericin content and chemical properties of Bivoltine and Multivoltine silkworm cocoon have not been studied yet. In this work, sericin was extracted from the silkworm cocoon of four different silkworm races at two different temperatures and durations. Significant differences were observed in yield% with different silkworm races and different treatments. Extracted sericin was characterized through SDS PAGE, FTIR & UV spectroscopy, and TGA.

Extraction and Biological Application of Silk Sericin: An Over View

The sericin is a byproduct which is obtained from silk industries. Mainly Bombyx mori is responsible for the synthesis of sericin. Sericin is proteinaceous in nature and is biodegradable. The two major components of silk i.e., fibroin and sericin have to be removed from raw silk to give it a luster or shine by degumming process. Silk sericin and its nutritive value has now been known to everyone in China and Japan. Many uses of sericin like pharmaceutical uses, textile -based uses, use in cosmetics has now been explored in India but the use of sericin as dietary supplement is not still discovered. Tons of silk has been produced in India and the degummed water goes as waste which can be used. Nanotechnological discoveries of sericin have given foremost advances in the field of biomedicine and tissue engineering, with special consideration the applications of a natural product for the enhancement of new pharmaceutical formulations and biomaterials. Sericin conjugated nano formulation are a vast example of nanotechnological tools applied to the blueprint of an biocompatible, economically viable, and biodegradable compound as well as its use as nanomedicine. The aim of this review is to highlight the application of sericin in different fields like in biomedical, food additives and the nano formulation of silk sericin.

Effectiveness of Samia cynthia ricini Boisduval (Lepidoptera: Saturniidae) Cocoon Extract as UV Protectant of Bacillus thuringiensis kurstaki in Controlling Beet Armyworm Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) under Sunl

Bacillus thuringiensis (Bt) is a biological agent for insect pest management. Its toxins effectively control Spodoptera exigua Hübner (Lepidoptera: Noctuidae) larvae, but it is sensitive to ultraviolet (UV) radiation from the sunlight. This study aimed to investigate the effect of sericin extract from Samia ricini Boisduval (Lepidoptera: Saturniidae) cocoons as a UV protectant for Bt after exposure under direct sunlight for 1, 2, 3, and 4 weeks. After being exposed to sunlight, the Bt formulae were tested against 20 larvae of 24 hr old, the first larval instar in the laboratory. The larval mortality was observed 72 hr after the treatment. The results indicated that the mortality of S. exigua in Bt + sericin extract treatment was significantly different compared with Bt alone. For the first week, the mortality of S. exigua in exposed Bt + sericin exposed Bt alone, unexposed (Bt + sericin, and unexposed Bt alone were 80, 61, 85, and 97%, respectively. Scanning electron microscopy analysis revealed that Bt + sericin, after being exposed to sunlight, still showed the presence of spore and crystal protein comparable to the unexposed Bt. Based on the results, sericin provides good protection against sunlight and prevents the Bt spores from light-induced damage.

Mushroom and silk sericin extract mediated ZnO nanoparticles for removal of organic pollutants and microorganisms

Mushroom and silk sericin extract mediated ZnO nanoparticles for removal of organic pollutants and microorganisms

Sericin eco-friendly biomaterial as a spin finish in drawn textured PET yarn production

Purpose This paper aims to extracted sericin from the cocoons of Bombyx mori silkworms, and sericin powder was applied onto drawn textured polyethylene terephthalate (PET) yarns as a spin finish. The reactivity on the surface of PET yarns was analyzed through Fourier transform infrared spectrophotometry–attenuated total reflectance (FTIR-ATR) and dyeing with methylene blue as a reactive dye. Also, investigations were conducted on the effects of sericin, citrc acid (CA) (as a crosslinking agent), and sodium hypophosphite (as a catalyst) concentrations on some properties of false-twist textured PET yarns. Design/methodology/approach A false-twist texturing machine (Scragg-Shirley minibulk, England) was used with the draw ratio of 1.05, heating temperature of 120°C, texturing speed of 100 m min−1 and applied twist of 3,000 TPM. The aqueous extraction of sericin was carried out by the boiling of the raw silk in distilled water with L:R: 40:1 for 120 min. The aqueous solution was filtered with a filter paper to remove the impurities and insoluble fibroin. Finally, the sericin solution was freeze-dried to obtain the sericin powder. The sericin solution was applied on the drawn textured PET yarns using the “pad-dry-cure” method. Findings Sericin fixation onto the PET yarns was confirmed by FTIR-ATR. The results showed that there were no significant changes in the tensile strength, linear density, crimp contraction and crimp modulus, elongation at break and shrinkage. In contrast, a substantial increase was observed in moisture regain, vertical wicking, dye uptake and ultraviolet protection. There was also a reduction just in the electrical resistivity, in the presence of sericin. Originality/value Although sericin has been known to have numerous beneficial properties, its application in textile industry as a spin finish has not been reported yet.

Study of chemical properties combination chemical method of wastewater treatment by methods IR-spectroscopy and X-ray diffraction

Complex formation processes of sericin compounds with Ni2+, Cu2+, Zn2+, Co2+ salts were studied. The possibility of maximum extraction of sericin was determined when wastewater is purified by the proposed method, and the developed purification method is recommended for amino acid purification of wastewater. By using the coagulants Al2(SO4)3•18H2O and FeCl3•6H2O and flocculant PAA together with the proposed sorbent, the concentration of coagulants in wastewater is 0.75 mg/l, 0.5 mg/l, 0.5 mg/l, and the amount of kaolin bentonite, respectively. An acceptable content of 2.0 g/l has been suggested. When the composition of the dry residue obtained after treatment with a composition consisting of bentonite, kaolin and aluminum sulfate was analyzed using X-ray structural analysis, it was found that the residue contained crystal spheres. The obtained results proved that the diffract grams of dry residue and bentonite samples are almost identical. It was found out in the research that these crystal spheres belong to the mineral contained in bentonite.

Dual-functional bioactive silk sericin for osteoblast responses and osteomyelitis treatment.

Sericin, a natural protein from silk cocoon, has been reported for various biological properties in the biomaterials field. Modified forms of sericin have been studied for bone tissue engineering, while its unmodified form has been scarcely reported. Therefore, the purpose of this study was to evaluate physical and biological properties of unmodified sericin for potential use in bone surgery. Sericin was extracted from silk cocoons using a chemical-free boiling method. Sericin extract showed distinct bands with molecular weight ranging from 25 to 42 kDa including smear bands. Fourier transform infrared spectra presented characteristic peaks of amide I, II, and III, confirming the chemical composition of sericin. Based on biological activity, sericin extract at a concentration of 40 μg/mL increased the proliferation of osteoblast cells up to 135%, compared with the untreated control. Moreover, increase in antibacterial activity against Staphylococcus aureus, both clinical isolates and the reference strain ATCC 29213, was demonstrated for sericin extract with normal saline, while no antibacterial activity was observed for sericin with broth. It was found that sericin with normal saline showed higher zeta potential than sericin without normal saline, indicating higher system stability. This was confirmed by the average particle size of sericin extract with NaCl (3,249.3±226.1 nm) showing approximately 10 times smaller than sericin solution (29,015.9 ± 8,085.6 nm). Furthermore, sericin extract at the minimal inhibitory concentration significantly reduced the biofilm formation of S. aureus up to 95%. The study indicates biological activities of sericin, which could be applied as a dual-functional bioactive material to support bone regeneration and treat bone infections.

Structure and Potential Applications of Polyamide 6/Protein Electro-spun Nano-fibrous Mats in Sorption of Metal Ions and Dyes from Industrial Effluents

The main objective of this work is the preparation of nano-fibrous mats (NFM) from polyamide 6 (PA6) reinforced and functionalized by renewable proteinic waste and examining their potential use for the sorption of heavy metal ions and dyes from wastewater from textile plants. Two renewable waste biopolymers; namely keratin (from waste of wool combing) and sericin (from degumming of natural silk) were extracted and regenerated. NFM were prepared by electro-spinning of PA6/ biopolymer composites in formic acid using nozzle-less electro-spinneret. The potential of the prepared mats for sorption of Cr+6, Cr+3, Cu+2, and Pb+2 cations as well as anionic and cationic dyes from wastewater was assessed. The prepared composites were characterized by measuring their viscosity, nano-fibre diameter, pore size, thickness, and air permeability. The morphological structure of the electro-spun NFM was investigated by scanning electron Microscopy. Chemical and physical properties of the obtained mat were studied FTIR, TGA, DSC, and XRD. The capacity of the prepared NFM for sorption of cations and dyes depended on the mat composition and the nature of the adsorbed cation or dye.
 This work meets some environmental and economic demand by extraction of keratin and sericin from waste materials and their utilization in reinforcement of PA6 NFM for sorption of selected cations as well as dyes from textile effluents.

Sericin from mulberry and non-mulberry silk using chemical-free degumming

In this study we report the extraction and characterization of sericin using water alone, from two non-mulberry silks - Antheraea assamensis (A. assamensis) and Philosamia ricini (P. ricini) compared with Bombyx mori (B. mori) silk. Degumming at 130 °C offered the highest sericin removal for A. assamensis; P. ricini had the lowest sericin removal among the varieties, because of its lower overall sericin content. Along with sericin degumming, fiber tensile properties were also reported. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of sericin revealed minimal sericin degradation even for degumming temperatures above 100 °C. The spectra from Fourier Transform Infrared Spectroscopy (FTIR) confirmed random coil motifs for B. mori sericin but a higher proportion of β-sheets in A. assamensis and P. ricini sericin which is likely to contribute to their lower water solubility. Thus, high temperature, water-based processing can be a potential approach for chemical- free degumming of silk fibers and to obtain high molecular weight sericin for different biomaterial applications.

Effect of protein and prebiotic on the survival of encapsulated probiotic during storage

The aim of this research was to investigate the impact of encapsulation system on its protective capability during freeze drying of Lactobacillus casei TISTR 1463 and its storage at 4˚C and 30˚C for 90 and 60 days, respectively. Sericin extract is a kind of protein with gel-like property that could aid in binding and also contains important amino acids. The combinations of sericin and prebiotics, including isomaltooligosaccharide and modified starch were evaluated during microencapsulation of probiotic L. casei TISTR 1463 in alginate beads. The highest encapsulation efficiency was 97.61% with the addition of sericinisomalto-oligosaccharide. Encapsulated probiotic with sericin-modified starch had the lowest specific rate of degradation (k) of 3.74x10-2 day-1 and 1.09x10-1 day-1 at both storage temperatures of 4˚C and 30˚C, respectively. Probiotic property of cell surface hydrophobicity (CSH) was also carried out.

EXTRACTION AND CHARACTERIZATION OF SERICIN PROTEIN FROM Bombyx mori

Silk sericin is a water-soluble macromolecular protein obtained from the raw cocoons of silkworm, Bombyx mori. It comprises of about 25% of the total cocoon shell weight and is categorized as a waste by-product in textile and silk industries after the extraction of silk fibers. This study emphasizes on the extraction of sericin from B. mori by a conventional salt alkaline method and characterized their physico-chemical properties, thermal stability, anti-bacterial and anti-oxidant activities. The extraction yield of the sericin protein from the cocoon was found to be 24 %. FTIR spectrum shows the presence of functional groups corresponding to the amino acids of sericin depicting its purity. TGA analysis demonstrates the degradation of sericin at 210 C with a weight loss of 57.69%. Sericin exhibited effective antibacterial activity against E. coli and S. aureus. DPPH assay revealed the antioxidant property of the sericin.

Extraction and antioxidant activity of sericin, a protein from silk

Abstract Sericin is a globular protein that represents 20% to 30% of the silk fiber from Bombyx mori silkworm cocoon. This protein is usually removed from the raw fiber and discarded by silk producers, a process known as degumming. However, sericin possesses significant biological properties that allows its application in various fields. The antioxidant activity is one of its most relevant benefits. Several authors have reported its anti-tyrosinase activity, lipid peroxidation inhibition and free radical neutralization. The antioxidant potential of sericin protein varies according to the extraction method used. Even though a wide variety of extraction techniques have been studied, simple technics including water at high temperature have exhibited efficient results. Furthermore, this method does not interfere with the safety of sericin for subsequent applications in food.

Structural and Functional Stabilization of Sericin from Bombyx mori Cocoons in a Biopolysaccharide Film: Bioorigami for Skin Regeneration

Development and optimization of a bioorigami film with silk sericin was pursued for skin regeneration. Several formulations were produced, with varying integrated sericin contents, viz. 0, 1, 2, 5, 10, 20 and 50 mgsericin mLfilm-1. The physico-chemical characteristics of the bioorigami films produced were evaluated via infrared spectrophotometry, X-ray diffraction, X-ray transmission, X-ray fluorescence, thermogravimetry, differential scanning calorimetry, transdermal protein permeation, kinetics of protein release from the bioorigami films and free radical scavenging activities. The mechanical resistance was also evaluated. The results by 2,2-diphenyl-1-picryl-hydrazil (DPPH) assay suggest that the bioorigami films integrating crude sericin extract had capacity to sequester free radicals, exhibited prolonged release of the bioactive protein, good physico-chemical characteristics and adequate mechanical resistance. Specifically, the bioorigami film with 10 mgsericin mLfilm-1 proved to be able to release all the bioactive protein in a 12 h timeframe. The results suggest potential for use in biopharmaceutical application, specifically, as material for use in hydrogels.

RETRACTION NOTE TO: The effects of sericin in recovering spermatogenesis and sexual hormone levels in diabetic rats.

Sericin-S (a hydrolysate from the silk protein sericin) is a natural antioxidant, which may improve spermatogenesis while having high biological safety, thus potentially useful for the treatment of male infertility. Our objective was to determine the effects of sericin-S on the sperm parameters and sexual hormone levels in a diabetic rat model. Thirty-six adult male rats were randomly divided in two groups, inducing diabetes in one of them by intraperitoneal administration of streptozotocin (STZ; 65 mg/kg). Both groups were randomly assigned to three subgroups, receiving oral gavage of saline, 1% or 2% sericin extract for 60 days. Therefore, the experimental design was a 2 × 3 factorial design of STZ and sericin treatments. One day after the last treatment, the rats were euthanized, weighed, the testes were processed (weigh, volume, and histology), and serum samples were processed for measuring sex hormone levels [testosterone, follicle-stimulating hormone, and luteinizing hormone (LH)]. STZ treatment decreased LH concentration and counts of spermatogonia, spermatocytes, and spermatids. Body and testis weights were significantly (p < 0.05) lower in the control group (non-diabetic saline) compared to the treated groups. In non-diabetic rats, sericin treatments increased (p < 0.05) the number of testicular cells (spermatogonia, spermatocytes, spermatids, Sertoli, and Leydig cells) and sex hormone concentrations. In diabetic rats, administration of sericin significantly (p < 0.05) improved sperm cell number and sex hormones levels. In nutshell, sericin can clearly modify sperm parameters and overall sex hormone function, and could improve spermatogenesis in normal and diabetic rats.