Separation Of Preservatives Research Articles

Separation of Preservatives in Cosmetic Products by Micellar Electrokinetic Chromatography

This paper describes the methodological optimization and validation of a simple micellar electrokinetic chromatography for the rapid simultaneous separation of 2-methyl-4-isothiazolin-3-one, methylparaben, ethylparaben, propylparaben, and butylparaben. By using a 30 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS), an applied voltage of 10 kV and a separation temperature of 40 degrees C, a quantitative determination was achieved, resulting in an analysis time of approximately 4 min. Only dilution and filtration are needed before analysis. The parameters for validation, such as linear ranges, detection limits, accuracy, and precision, are also reported.

Comparison of Extraction Methods and Column Types for the Determination of Additives by Liquid Chromatography

Abstract Several extraction methods and column types were compared, with respect to extraction and separation efficiency of 14 additives from soy sauce, sugared fruit and dried roast beef, by paired-ion liquid chromatography. Results showed that the application of a Sep-Pak C18 cartridge was the best method for extraction, because it resulted in higher recovery than those given by acetone extraction and steam distillation. The purification of acetone extract by a Sep-Pak silica gel cartridge can result in recovery loss. Monomeric column was superior to polymeric column for simultaneous separation of preservatives, antioxidants and sweeteners. The capacity factor (k′) of each additive was also determined.