Separate Areas Research Articles

Copper oxide/biopolymer nanocomposites: synthesis and applications, a comprehensive review

Copper oxide particles have a significant role in various fields due to their many properties like special shape, size, and high surface area. Due to their rarity and unique characteristics, such as their large surface area, paramagnetic nature, and ease of separation, Copper oxide nanoparticles have received the greatest attention. Chitosan, Guar Gum, Tamarind, Alginate, starch, cellulose, polysaccharide, etc. are examples of natural biopolymers that have proven to be excellent hosts for the creation of CuO nanoparticles. Long-established fabrication techniques for biopolymer-based CuO nanocomposites include co-precipitations, green synthesis, Solvent Casting Method, Alco thermal method, and Sol-Gel methods. Excellent biological characteristics of Copper oxide/biopolymer nanocomposites include their potent antibacterial activity against a variety of diseases as well as bacteria that are resistant to antibiotics. These characteristics have sparked the creation of numerous strategies with direct biological applications, including customized surfaces with antimicrobial effects, wound dressings, and modified textiles. This study aims to provide the very first biopolymer CuO nanoparticles to be reported in the previous ten years as well as its appealing methodology in diverse applications.

Individualistic reward-seeking strategies that predict response to nicotine emerge among isogenic male mice living in a micro-society.

Individual animals differ in their traits and preferences, which shape their social interactions, survival, and susceptibility to disease, including addiction. Nicotine use is highly heterogenous and has been linked to the expression of personality traits. Although these relationships are well documented, we have limited understanding of the neurophysiological mechanisms that give rise to distinct behavioral profiles and their connection to nicotine susceptibility. To address this question, we conducted a study using a semi-natural and social environment called "Souris-City" to observe the long-term behavior of individual male mice. Souris-City provided both a communal living area and a separate test area where mice engaged in a reward-seeking task isolated from their peers. Mice developed individualistic reward-seeking strategies when choosing between water and sucrose in the test compartment, which, in turn, predicted how they adapted to the introduction of nicotine as a reinforcer. Moreover, the profiles mice developed while isolated in the test area correlated with their behavior within the social environment, linking decision-making strategies to the expression of behavioral traits. Neurophysiological markers of adaptability within the dopamine system were apparent upon nicotine challenge and were associated with specific profiles. Our findings suggest that environmental adaptations influence behavioral traits and sensitivity to nicotine by acting on dopaminergic reactivity in the face of nicotine exposure, potentially contributing to addiction susceptibility. These results further emphasize the importance of understanding interindividual variability in behavior to gain insight into the mechanisms of decision-making and addiction.

3D model of a stable triangle LiF–NaBr–KBr four-component reciprocal system Li+, Na+, K+ || F-, Вr-

A 3D model of the phase equilibrium states of the quasi-three-component system LiF–NaBr–KBr, which is a stable triangle of the four-component reciprocal system Li+, Na+, K+ || F-, Br-, has been constructed. Based on the 3D-model, polythermal, isothermal sections and the polytherm of phase crystallization were constructed for the first time. Two polythermal sections contain wide areas of boundary solid solutions based on sodium and potassium bromide. In an isothermal section at 650 оC, the fields of the liquid phase and the coexisting two and three phases are delimited. The crystallization polytherm is represented by three fields. In the crystallization field of lithium fluoride, the area of separation of two liquids is limited. The direction of the ion exchange reaction 2LiBr + NaF + KF = 2LiF + NaBr + KBr was confirmed by thermodynamic calculations at temperatures of 400, 600, 800, 1000K. The exothermic nature of the exchange reaction is confirmed by taking a DTA heating curve for a mixture of powders from 50% LiBr + 25% NaF + 25% KF, and the phase composition of the reaction products LiF + NaBr(OTR) + KBr(OTR) is confirmed by X-ray phase analysis data, where OTR is limited solid solution.

The associations between mathematical skills, cognitive performance, and language background in elementary school children. a two-year follow-up study

ABSTRACT Early mathematical skills contribute to later school performance and socio-economic status. Working memory is related to mathematical skills, but spatial and language skills have independent effects on separate areas of mathematical skills such as solving word problems or arithmetic skills. In this study, 9- to 10-year-old children's (N = 57) cognitive and mathematical skills were assessed three times over 2 years. Spatial reasoning was associated with performance in solving word problems and working memory with performance in the arithmetic test. Regarding word problems, girls outperformed boys. Also, the second-language learners performed similarly to the native speakers in the beginning and in the end of the third grade, but worse in the end of the fourth grade. The results suggest that the students’ inadequate mastery of the school language may lead to underachievement in mathematics, implying that further efforts should be invested in supporting their language learning.

Utilization of stable isotopes in teeth to investigate pygmy sperm whales (Kogia breviceps) in the southeastern United States

AbstractThe pygmy sperm whale (Kogia breviceps) is currently the second‐most stranded cetacean in the southeastern United States (SEUS), but information concerning their population structure is severely limited. This study used stable isotope analysis to investigate the isotopic niches and population structure of K. breviceps among three regions in the SEUS determined by the distance of the continental shelf break and a fourth region in the Gulf of Mexico. Teeth from stranded individuals were subsampled via dental drill at four separate areas on each tooth, representing four different age classes: calf, juvenile, subadult, and adult. Stable isotope ratios of carbon and nitrogen were measured in the organic component of 42 teeth, and oxygen isotope ratios were measured in the inorganic (hydroxyapatite) component of 19 teeth. No differences were found in δ13C, δ15N, or δ18O values between regions. However, differences between age classes were significant for adults and subadults for δ13C and adults and juveniles for δ15N. Differences between males and females were also significant for δ15N. Results from this study provide the first carbon and nitrogen isotope values from different age classes of pygmy sperm whales as well as the first reported oxygen isotope values for this species.

A morphological and high throughput sequencing workflow to identify Australian ants (Hymenoptera, Formicidae): a new tool for biosecurity and biodiversity

Ants are often the most ubiquitous and ecologically influential components of terrestrial systems, exhibiting an exceptionally high level of endemic diversity. Simultaneously, exotic ants can be amongst the most environmentally and economically devastating biological invaders. Distinguishing between exotic and related natives is essential for early detection and to reduce the chance of establishment, but at the species-level there remains a great deal of uncertainty among several of the most diverse, widespread and ecologically dominant genera. This taxonomic impediment negatively impacts the use of molecular techniques relying on reference databases, due to the poor state of ant DNA sequences in publicly available repositories, with many species not represented at all and others incorrectly identified. As a result, biosecurity screening for targeted exotic ants and assigning ant species-level identifications for biodiversity remain two separate areas of focus in Australia. Here we propose a workflow for the identification of ants that enables simultaneous processing of large numbers of “bulk” ant samples each containing many individuals, increasing resolution for taxonomic assignment, and generating a curated database linked to voucher specimens. We use a non-destructive DNA extraction method and compare two high throughput sequencing (HTS) metabarcoding platforms – MiSeq (Illumina) and MinION (Oxford Nanopore Technologies) – processing up to 180 bulk mixed ant samples per run. This approach allowed for the acquisition of curated DNA sequences from voucher specimens morphologically examined by expert taxonomists. This work highlights the advantages and current limitations of DNA-based identifications, the needs for standardisation, as well as the importance of a taxonomy-based curation of DNA database for both biodiversity and biosecurity.

Sneaky DCIS-looking Invasive Ductal Carcinoma of the Breast in the Extensive DCIS Background

Abstract Introduction/Objective In the most cases, invasive ductal carcinoma (IDC) of the breast is identifiable when they present with classic infiltrative growth pattern. However, subset of IDC can occur in a very sneaky way, significantly mimicking the appearance of ductal carcinoma in situ (DCIS). In this condition, it’s much more easier to miss the invasive component without pulling ancillary staining when morphologic findings are extremely compatible with DCIS, especially the diagnosis of DCIS was made on the previous biopsy. Methods/Case Report Retrospective analysis of the histologic and immunohistochemical findings of pure DCIS and DCIS-looking invasive ductal carcinoma. Results (if a Case Study enter NA) Here, we reported a 55 year-old female who was noted to have microcalcification at the 11:00 o’clock of the right posterior breast on routine mammographic examination in 09/2023. Biopsy of the calcification area in 10/2023 reported high grade DCIS (ER+ PR-). Histologic examination of subsequent mastectomy specimen showed two separate DCIS-looking areas. Immunohistochemical (IHC) staining showed that myoepithelial markers, p63 and smooth muscle myosin heavy chain (SMMHC), were retained at the periphery of all the expanded acini in one area. Unexpectedly and surprisingly, p63 and SMMHC were completely lost at the periphery of part of the DCIS-looking acini in another area, immunohistochemically compatible with the diagnosis of invasive ductal carcinoma admixed with DCIS. Conclusion Knowing that invasive ductal carcinoma of the breast can present as DCIS-looking morphology, especially given the condition that the diagnosis of DCIS was rendered on the previous biopsy, will enhance awareness of pathologists to recognize sneaky DCIS-looking invasive ductal carcinoma in the extensive DCIS background. In turn, this will prevent misdiagnosis and under-treatment of patients with invasive ductal carcinoma of the breast.

Quality Determination of Dairy Farm Wastewater in Dinajpur

At present environmental pollution is a talked about issue. Due to environmental pollution, humans and animals face threats. The scientist has pointed out that waste is one reason for climate change. Solid, liquid, gaseous etc. are different types of waste. The experiments were conducted to determine the chemical constituents present in dairy farm wastewater, wastewater management practice and environmental impact and compare it with the groundwater Dinajpur Sadar upazila. The data concerning the dairy farm wastewater in Dinajpur was obtained through a designed questionnaire, and separate area inspection interacting with the proprietor and workers in the dairy farm. Randomly collected samples from different dairy farms. The chemical constituents of the wastewater sample were determined by a laboratory experiment. The wastewater contained Mg, Na, Ca, Cl-, K, EC, P, HCO3-, pH, TDS, DO, COD, Zn, S, BOD, HT and NO3-. The Na, K, Ca and pH concentration of wastewater under the range in groundwater in Dinajpur. However, concentrations of P, TDS, Mg, EC, Cl-, HCO3- and HT are above the groundwater range in Dinajpur. The produced wastewater was disposed of either through drainage or piping systems on fellow land, ponds, open lakes, roadside land, urban drains, and rivers. The unplanned wastewater disposal creates bad odor, and environmental pollution, seduces the growth of mosquitoes decreases the water quality, soil quality and health hazards. Therefore, it can be concluded that the prevailing dairy farm wastewater disposal system not being satisfactory. The proper disposal system should be improved to reduce environmental impacts.

Interaction of International Humanitarian Law and International Human Rights Law in the context of armed conflicts

It is indicated that the problems of the interaction of international humanitarian law and international human rights law lie in the different historical origins, evolution and application of these two branches of law. The complexity of the interaction between the IHL and the ICRC lies in their extraterritorial action and different personal spheres of application. IHL undeniably applies to international and non-international armed conflicts, regulating the conduct of states and armed groups, while the IHL is recognized as the basis of law in peacetime, but also applies in periods of conflict. Finding the points of intersection between international human rights law (IHRL) and international humanitarian law (IHL) in the context of armed conflicts is the main objective of this section. The merger of these two legal fields, traditionally divided into separate areas of responsibility in peacetime and wartime, has gained unprecedented importance in the legal analysis of modern conflicts. The impetus for the development of the new paradigm was the rapid evolution of the nature of war, characterized by the emergence of asymmetric warfare and the blurring of the boundaries between state and non­state actors. Such transformations in the theater of war require a nuanced understanding of the legal instruments used in this context, which emphasizes the need for this analysis. The urgency of the issue is emphasized by the changing dynamics of international practice. The recognition by the International Court of Justice of the continued applicability of IHRL in conflict and the definition of IHL as lex specialis emphasizes that the legal landscape is in constant flux. At the heart of this analysis is the difficulty of determining the applicability of IHL, a task that is complicated by the problems of classifying conflicts as international or non-international. The principle of derogation - whereby states may temporarily suspend certain human rights obligations - creates an additional layer of complexity, affecting the interpretation and application of laws during armed conflict. Many provisions of international human rights conventions define the protected interest and then provide that any restrictions must be justified by reference to a list of aims, which varies from article to article and treaty to treaty, that are necessary for the achievement of the aim in question and are proportionate to its achievement. In this way, the IHRL provides a mechanism for balancing the requirements of individuals with those of others or the community itself.

A method for numerical simulation of shock waves in rarefied gas mixtures based on direct solution of the Boltzmann kinetic equation

The paper proposes an approach to numerical simulation of shock waves in rarefied gas mixtures on the basis of direct solution of the Boltzmann kinetic equation. Software for simulating the gas flows was developed. The structure of a shock wave in a binary gas mixture was computed with an accuracy controlled by the computational parameters. The computations were performed for various molecular masses ratios and Mach numbers. The total accuracy of at least 1.4% for the local values of the molecular densities and temperatures of the mixture components was achieved. Numerical simulation of a shock wave propagation through a periodically perforated surface was performed. The distributions of the macroscopic characteristics of the mixture components at various points in time were obtained. Unsteady areas of strong separation of the gas mixture components were discovered.

Об изменении подхода к правовому регулированию проведения оперативно-розыскных мероприятий

The article analyzes proposals to improve operational investigative legislation, for example, proposals to improve the purpose and objectives of the Police Department, to change the system of operational investigative measures. A separate area of improvement of the system of norms should be called the coordination of operational investigative measures in relation to solving specific tasks of the ORD. The article concludes that operational investigative activities in relation to each area in which they are conducted are different. In some cases, all operational investigative capabilities are needed, and, accordingly, the right to conduct all operational investigative measures should be granted. In other cases, all the capabilities of the HORDES are not required, all tasks can be solved with a limited amount of funds. This thesis was verified by questioning the operational staff. The paper describes the results of the study, which provides data on the demand for individual MPas in relation to the directions of the MPas.

On the demarcation of ecolinguistics

Abstract This article discusses the demarcation of contemporary ecolinguistics. It takes a starting point in different conceptualisations of the field, including (1) the claim that ecolinguistics is an umbrella term for work that attends to interconnections and dynamics in multiple contexts, (2) the claim that ecolinguistics comprises two traditions, a Haugenian tradition and a Hallidayan tradition, and (3) the claim that ecolinguistics consists of four strands that orient to the symbolic, sociocultural, natural, and cognitive ecology of language, respectively. Using bibliometric methods, the article concludes that there is currently no unity in ecolinguistics that warrants the first claim. Further, it concludes that there is no bibliometric or empirical evidence for the second claim, as there is virtually no connection between the two traditions, and hence they are two separate research areas, rather than two traditions within one field. Finally, it is shown that while there do indeed exist four ecological frameworks in linguistics, they are not subfields within ecolinguistics. It is concluded that contemporary ecolinguistics is defined by its preoccupation with natural ecologies. Based on this conclusion, it is suggested that the ecolinguistic preoccupation with natural ecologies presupposes treating language as integral to living and the ecology.

Maleimide-induced crosslinking of polyimide membranes for high gas separation performance and plasticization resistance

Trade-off effect and plasticization are the key issues confronted in the area of membrane separation when dealing with applications involving high-pressure, soluble gases like CO2. In this study, a novel diamine (TAPA-MAA) was synthesized derived from tris(4-aminophenyl)amine (TAPA) and maleic anhydride (MA). This diamine was copolymerized with DAM (2,4,6-trimethylbenzene-1,3-diamine) and 6FDA (4,4′-(hexafluoroisopropylidene)diphthalic anhydride) to form the polyimide (PI) membrane containing maleimide (MI) cross-linking sites via chemical imidization way. Then, under the action of heat treatment, the crosslinked PI (cPI) membrane was constructed since the MI crosslinking initiates a radical cycloaddition reaction to form a succinimide-linked structure, and named as cPSI membrane for short. Further, an increase in the ratio of TAPA-MAA gives rise to the resultant cPSI membrane with elevated cross-linking density. The obtained cPSI membranes showed significantly enhanced gas selectivities and superior anti-plasticization property compared to the un-crosslinked PI membranes due to the MI-induced crosslinking reaction in the PI main chain, resulting in a marked increment (24 %) in ultra-microporosity (<7 Å) and a reduction in the d-spacing value (6.10 Å vs 5.80 Å), generating a strong molecular sieving effect. Meanwhile, the highly interconnected covalent structure formed by the MI-induced crosslinking will suppress the plasticizing phenomenon induced by high-pressure soluble gases. The typical membrane (cPSI-3:7-350) possessed great CO2/CH4 separation performance, CO2 permeability of 334.4barrer and a CO2/CH4 selectivity of 61.9 that is exceeded the 2008Roberson upper bound, along withCO2 plasticization pressures above 44 bar. Besides, its mixed CO2/CH4 separation performance exceeded the 2018 upper gas mixture limit when the upstream pressure changed from 2-20 bar. This study provides valuable insights for the design of PIs containing MI cross-linking sites, which can enhance the performance of membrane-based gas separation.

Secukinumab (Cosentyx)

It is recommended that Cosentyx be reimbursed by public drug plans for the treatment of adult patients with moderate to severe hidradenitis suppurativa (HS) (acne inversa) who have responded inadequately to conventional systemic HS therapy only if certain conditions are met. Cosentyx should only be covered to treat patients who have moderate to severe HS (Hurley stage II or III), a total abscess and nodule count of 5 or greater, and lesions in at least 2 separate areas of the body. Additionally, these are patients whose HS did not adequately respond to conventional therapy. Cosentyx should only be reimbursed if prescribed by a physician experienced in the management of HS but should not be reimbursed if used in combination with other biologic therapies for HS. Cosentyx should be reimbursed for ongoing treatment if there is improvement in HS after starting treatment with Cosentyx. The price of Cosentyx should be negotiated so that its total drug cost does not exceed the total drug cost of treatment with the lowest-cost adalimumab.

Postnominal flagging and OV in Sinitic

Abstract Sinitic languages are known for their SVO order and mostly isolating morphology. This study addresses eleven languages of four different areas in Hunan, Yunnan, Sichuan, and Qinghai/Gansu provinces in China that possess SOV order and developed a postnominal flagging system. This study presents a synchronic description as well as a diachronic analysis of the flagging systems in these languages, focusing on typological properties (ergative or secundative alignment) and the role of language contact. The existence of four separate areas with limited mutual contact allows a contrastive approach and inferences on the role of different contact languages (Tibetic, Mongolic, Tujia) or different types of language contact (borrowing of flags, shared grammaticalization). The study argues against OV order and postnominal flagging as defining features of the “Amdo Sprachbund”, showing that these are universally present in all four areas and are better understood as the result of contact between two Eurasian macro areas.

Legal and Economic Analysis of Fair Use in the Process of Artificial Intelligence Creation in Data-Driven Era

In the era of digitalization, AI-generated content has emerged as a pivotal component within the creative industries, thereby instigating extensive deliberations concerning copyright protection and fair use. This study aims to scrutinize and analyze the equitable use of AI-generated content from both a legal and economic standpoint. This paper does a thorough review of the literature to introduce basic ideas about AI content creation and fair use. It then divides existing research into four separate areas: technical analysis, legal case analysis, economic impact analysis, and policy adaptation research. Despite notable advancements in both technological capabilities and legislative frameworks surrounding AI-generated content, there remains a dearth of comprehensive understanding, particularly with regard to its economic implications and policy implementation. Consequently, this study posits a significant research question on how to strike a balance between the fair usage of AI-generated content and upholding copyright protection in order to foster sustainable development within the creative industries. The findings presented herein offer novel perspectives and insights that can inform future policymaking endeavors as well as academic investigations.

COMPARATIVE ANALYSIS OF METHODS AND MODELS FOR ESTIMATING SIZE AND EFFORT IN DESIGNING MOBILE APPLICATIONS

Mobile applications have long become an integral part of everyday life and have gained popularity among ordinary users, small companies, and large enterprises. However, this is a completely separate area of software. We can say that this is a whole separate ecosystem. Creating functional software for this ecosystem comes with new requirements, constraints, and characteristics that were not used in previous software metrics evaluation methods such as desktop programs or web applications. Mobile applications have new features, limitations and capabilities that are different from other software. Estimating the size and effort helps the software development team approximate the costs needed to more successfully develop a software product such as a mobile application. An early estimate of size and effort is necessary to identify resources that can help in the early stages of implementation a software project within budget and on time. The reliability of the assessment is also of great importance. Low reliability can lead to overestimation or underestimation, which can have bad consequences for any software development company, especially mobile applications. However, the suitability of methods and models for measuring size and efforts that are used for traditional software (PC applications and web applications) do not always provide sufficient reliability for mobile applications.The main goal of this article is to analyze the usage of various methods and models for estimating size and effort the development of mobile applications, including for the Android OS at the initial stages of their design. There is a brief description of the main and widespread methods and models that are used to estimate the size and effort, such as: Lines of Code (LOC), Function Points (FP), COCOMO and COCOMO 2, COSMIC, FiSMA, application of regression analysis for construction of mathematical models. The methods and models proposed by various authors are directly analyzed.

Substance use disorder risk assessment: positive emotional experiences with first time use and substance use disorder risk.

Substance Use Disorder (SUD) screening tools used in current practice are designed to identify SUD once patients have begun regular dangerous drug use. While these screening tools are valuable, prevention and avoidance of SUD would save countless lives. The climbing number of deaths due to drug overdose make screening for and prevention of SUD imperative. This study addresses this care gap. The aim was to develop a simple screening tool for patients who may be prone to develop Alcohol Use Disorder (AUD) and/or SUD prior to addiction. It was hypothesized that participants with initially positive emotional experiences would be correlated with a future SUD diagnosis. The study involved a self-administered survey using a cross-sectional design and was carried out over one-month in the spring of 2021. Those patients who presented to the MAT clinic (SUD group) were seen in a separate area than the patients presenting for urgent care (Comparison group). Participants (N=259) were voluntarily recruited from MAT and Urgent care: Patients receiving acute care were assigned to the Comparison (N = 126, 50.8% female, 5.7% non-white, 27.2% age < 34) and those receiving treatment for SUD were assigned to the MAT group (N =133, 40.8% female, 4.8% non-white, 36.8% ≤34). The survey questioned demographics (4 items), risk factors for AUD/SUD (6items), information about first alcohol/opioid experiences (16 items), and factors for seeking AUD/SUD treatment and recovery (2 items). Feelings were categorized as positive (e.g., euphoria, happiness, self-confident), neutral (e.g., nothing, normal), or negative (e.g., depressed, sad, sick). The MAT group felt more positive feelings with first usage of alcohol and opioids compared to the comparison group (p<.001). With first usage of opioids specifically, MAT (0.13 ± 0.04) and comparison (0.29 ± 0.07) groups differed (p <.001). Over half (55.3%), of the MAT participants reported feeling self-confident with first use of alcohol while only 29.7% of the comparison reported this (p<.001). Over three-fifths (63.7%) of the MAT group reported feeling of euphoria with the first usage of opioids compared to one-tenth (9.8%) in the comparison group (p<.001). This retrospective cross-sectional report shows the first affective responses to substances may predict risk for future SUD and could be a prevention screening tool. Asking patients about positive feelings with first usage of alcohol/opioids could be a simple screening tool employed for prevention.

Study on Production Characteristics and Change in Production Location of Pottery Excavated from DongOe-dong Site in Goseong

As a result of scientific analysis of 26 pottery excavated from the DongOe-dong site in Goseong, pottery with various firing degrees was confirmed, ranging from low-firing pottery with mica minerals detected to high-firing pottery with high-temperature minerals such as cristobalite and mullite detected. In addition, low-firing pottery has an absorption rate of 9.7∼25.8% and generally shows high saturation value. On the other hand, high-firing pottery has low absorption rate and saturation value, so the physical characteristics of the pottery show a high correlation with the group classified by XRD analysis. Pottery with a low firing degree commonly contains fine-grained mineral particles and has a similar composition of trace elements such as Rb, Ba, and Sr. And they were most abundantly excavated from the No. 1-1 moat. However it was confirmed that there is a difference in the production area because compositions of the rare earth elements are distributed in two areas in the graph. High&amp;intermediate-firing pottery samples are observed to have a vitrified base matrix and relatively less temper minerals. And their contents of Rb, Sr, and Ba are significantly different from low-firing pottery. In the composition of rare earth elements, they are all distributed in same area as one of the separated areas of low-firing pottery, so it is highly likely these pottery were produced in the same or nearby production region regardless of the firing degree. As a result of checking up the composition of rare earth elements according to the archaeological chronology of pottery, it is estimated that there was a change in the production region of the pottery introduced into the DongOe-dong site around 250 AD.

Barriers and enablers: How logistics companies could tackle the transition to electrified road freight transport

Electrifying road freight transport can heavily reduce the sector’s climate impact; however, there exist many uncertainties for making a large-scale transition, especially for logistics companies. In response, the purpose of this article is to identify and describe barriers to transition, but also strategies for how the barriers can be managed. To address the purpose, an interview study was carried out together with two complementary workshops to capture the views of relevant actors. Barriers to electrification are clustered into four separate areas: practical and technological barriers, financial barriers, institutional barriers, and social and cultural barriers. Low cost and high logistics performance are viewed as logistics companies’ competitive advantages, but they are expected to be challenged when making the transition. The current structure of the transport chain also further complicates things: it is transaction-based and risks and benefits can occur in its different parts. This is an issue, since the actor that is forced to take risks is not always the actor that benefits the most. One way to manage this is for actors to form more long-term commitments through collaborations. Moreover, it can be advantageous to start electrifying subsystems that can be described as ‘closed’ and ‘static’, i.e. predictable and easy to control, such as transport between terminals. At the other end of the spectrum, subsystems that are ‘open’ and ‘dynamic’ are the ones that are most difficult to electrify.