Sensors For Detection Of Biomarkers Research Articles

Chronopotentiometric Nanopore Sensor Based on a Stimulus-Responsive Molecularly Imprinted Polymer for Label-Free Dual-Biomarker Detection.

The development of sensors for detection of biomarkers exhibits an exciting potential in diagnosis of diseases. Herein, we propose a novel electrochemical sensing strategy for label-free dual-biomarker detection, which is based on the combination of stimulus-responsive molecularly imprinted polymer (MIP)-modified nanopores and a polymeric membrane chronopotentiometric sensor. The ion fluxes galvanostatically imposed on the sensing membrane surface can be blocked by the recognition reaction between the target biomarker in the sample solution and the stimulus-responsive MIP receptor in the nanopores, thus causing a potential change. By using two external stimuli (i.e., pH and temperature), the recognition abilities of the stimulus-responsive MIP receptor can be effectively modulated so that dual-biomarker label-free chronopotentiometric detection can be achieved. Using alpha fetoprotein (AFP) and prostate-specific antigen (PSA) as model biomarkers, the proposed sensor offers detection limits of 0.17 and 0.42 ng/mL for AFP and PSA, respectively.

Breath biosensing: using electrochemical enzymatic sensors for detection of biomarkers in human breath

Abstract Detection of biomarkers for disease by noninvasive methods is critical for the early diagnosis and screening of disease, enabling prompt treatment. Breath biosensors are a viable option as the exhaled breath contains several biomarkers linked to lung cancer, oxidative stress, diabetes, and other diseases. Breath analysis has been achieved by advanced analytical techniques such as gas chromatography and infrared spectroscopy. However, electrochemical enzymatic breath biosensors offer a cost-effective, sensitive platform for biomarker detection without complex analysis and interpretation by trained laboratory personnel. This review aims to summarize recent advances in the field of electrochemical enzymatic breath biosensors and offer future opportunities from other applications of nonelectrochemical enzymatic breath biosensors.

Polyaniline assisted USB based sensor for determination of benzene biomarker

Benzene is a known Hemotoxic carcinogen for humans. The carcinogenic properties justify that any exposure to Benzene in ambient air needs to be monitored. Exposure to Benzene is indicated by presence of trans-trans Muconic Acid (ttMA) in urine of human as a biomarker. Currently the levels of biomarker are measured by sophisticated and costly equipment like High Precision Liquid Chromatography (HPLC). This commercial procedure is time consuming due to rigorous sample preparation, involvement of expensive equipment and need of expertise for analysis. To remedy this, USB assisted and Polyaniline (PANI) based point of care electrochemical sensor for detection of biomarker of Benzene is developed. To operate this system, one does not require sample preparation and trained hands.The system is USB based portable potentiostat which performs Linear Sweep Voltammetry (LSV) for detection of ttMA. The design comprises of potentiostat interfaced with microcontroller FRDM-KL 25Z of NXP semiconductors. The system detects nanoampere current with linear sweep voltage resolution of 800 μV. Graphical User Interface (GUI) facilitates to set the parameters like start potential, end potential, step size and scan rate of linear sweep voltage. The real time serial plotter equips the viewing and capture of real-time sensor data from electrochemical cell. The three-electrode electrochemical cell consists of PANI coated Aluminum as working, Graphite Pencil Electrode (GPE) as counter, and Saturated Calomel Electrode (SCE) as reference which is utilized for electrochemical detection of ttMA in urine sample.This system is capable of detecting ttMA in Urine which corresponds to intermediate (1-5 ppm) and high (>5 ppm) level exposure of Benzene in ambient air.

Current Developments and Potential Applications of Biosensor Technology

The development of various new kinds of sensors for the accurate detection of biomarkers in biological fluids and environmental samples are of greatest importance for the early diagnosis of diseases and to avoid the contamination of environment through pollutants, toxic and biohazardous materials. Sensitivity limits of biosensor have increased due to developments of new biological methods like tagging of fluorescence molecule with nanomaterials. Moreover, usage of peptide arrays, aptamers, antibodies, nucleotides and molecule fixed polymers, facilitate to improve advanced biosensors over conventional approaches. Several biosensors ranging from nanomaterials, polymers to microbes have broader potential applications. Generally, biosensor has been organized into several categories containing diverse sensing arrangements such as mechanical, optical and electrical transducers and modern biosensors use micro- and nanofabrication tools, as either label-free or labeled. This review provides an overview of recent developments and applications of biosensors in the fields of biomedical sciences and environmental monitoring, along with the better detection limit and improved sensitivity of the biosensors.

Electrochemical Sensors for Detection of Biomarkers of Exposure to Pesticides and Nerve Agents

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