Sensitive Ecosystems Research Articles

CONSERVAÇÃO DA GEODIVERSIDADE EM GEOSSISTEMAS FERRUGINOSOS

This study examines the evolution of areas surrounding the bat caves in the Ferruginous Geosystem of Carajás from 1985 to 2022, emphasising the significance of geodiversity conservation in this unique context. The land use and cover transformations analysis reveals critical insights into spatio-temporal changes and escalating environmental degradation risks. The findings enhance the literature on geodiversity conservation and speleology, providing a solid foundation for future research. Monitoring mined areas and their ecological impacts is essential for refining management strategies for these sensitive ecosystems. Furthermore, recent attempts to amend cave protection legislation, particularly Decree 10.935, pose significant conservation challenges, necessitating a critical evaluation of environmental policies and fostering dialogue among researchers, policymakers, and the mining industry. This study highlights the urgent need for a balanced approach between resource extraction and environmental conservation to mitigate adverse impacts on biodiversity and ecological processes. Integrated and evidence-based environmental management is crucial for addressing the challenges of mining activities and regulatory changes, ensuring the protection of caves and their ecosystems for a sustainable future.

Global influence of soil texture on ecosystem water limitation.

Low soil moisture and high vapour pressure deficit (VPD) cause plant water stress and lead to a variety of drought responses, including a reduction in transpiration and photosynthesis1,2. When soils dry below critical soil moisture thresholds, ecosystems transition from energy to water limitation as stomata close to alleviate water stress3,4. However, the mechanisms behind these thresholds remain poorly defined at the ecosystem scale. Here, by analysing observations of critical soil moisture thresholds globally, we show the prominent role of soil texture in modulating the onset of ecosystem water limitation through the soil hydraulic conductivity curve, whose steepness increases with sand fraction. This clarifies how ecosystem sensitivity to VPD versus soil moisture is shaped by soil texture, with ecosystems in sandy soils being relatively more sensitive to soil drying, whereas ecosystems in clayey soils are relatively more sensitive to VPD. For the same reason, plants in sandy soils have limited potential to adjust to water limitations, which has an impact on how climate change affects terrestrial ecosystems. In summary, although vegetation-atmosphere exchanges are driven by atmospheric conditions and mediated by plant adjustments, their fate is ultimately dependent on the soil.

Pervasive Microplastic Ingestion by Commercial Fish Species from a Natural Lagoon Environment

Microplastics have emerged as a significant global environmental concern in the recent decade. The aim of this study was to elucidate microplastic contamination of commercial fish species in a natural lagoon environment. Microplastic contamination was examined in the gastrointestinal tracts and gills of 157 commercial fish from 18 species with varying feeding habits in a vital and sensitive lagoon ecosystem, which connects to the Indian ocean. Microplastics were extracted using digestion, followed by stereomicroscopic inspection using Nile Red stain, and identified via μ-FTIR analysis. Over half of studied fishes ingested microplastics (54.14%). Filaments (50%) and blue items (43%) were the most commonly ingested. Of all the fish species, Eubleekeria splendens had the highest average concentration of microplastics in GIT (1.41 ± 2.52 items/g w.w. tissues), although no statistically significant difference in amount of ingested microplastics (items/g w.w. tissues) was observed among species. The highest concentrations of inhaled microplastics were recorded in Sillago vincenti (1.38 ± 1.30 items/g w.w. tissues). The majority of the extracted microplastics (33%) belonged in the size class 500–1500 μm with rayon, polyethylene terephthalate, and polypropylene as the primary polymers. This study found no correlation between microplastic ingestion and fish species and feeding habits, but a positive correlation with fish size was observed. These findings reveal widespread microplastic contamination in edible fish, posing potential risks to commercially important species due to increasing pollution in lagoon ecosystems.

Analysis of Spatial and Temporal Trends of Vegetation Cover Evolution and Its Driving Forces from 2000 to 2020—A Case Study of the WuShen Counties in the Maowusu Sandland

The WuShen counties in the hinterland of the Maowusu Sandland are located in the “ecological stress zone” of the forest–steppe desert, with low vegetation cover, a strong ecosystem sensitivity, and poor stability under the influence of human activities. Therefore, it is important to study and analyze the changes in vegetation growth in this region for the purpose of objectively evaluating the effectiveness of desertification control in China’s agricultural and pastoral intertwined zones, and formulating corresponding measures in a timely manner. In this paper, the spatial and temporal variations in the vegetation NDVI in the WuShen counties of the Maowusu Sandland and their response relationships with driving factors were investigated by using a trend test, center of gravity transfer model, partial correlation calculation, and residual analysis, and by using the MOD13A3 vegetation NDVI time series data from 2000 to 2020, as well as the precipitation, temperature, and potential evapotranspiration data from the same period. The results showed the following: ① The regional vegetation NDVI did not fluctuate significantly with latitude and longitude, and the NDVI varied between 0.227 and 0.375 over the 21-year period, with a mean increase of 0.13 for the region as a whole and an increase of 0.61 for the region of greatest change. Of the area, 86.83% experienced a highly significant increase, and the trend in increase around rivers and towns was higher than that in the northwestern inland flow area, with the overall performance of “low in the west and high in the east”. ② Only 2.07% of the vegetation NDVI center of gravity did not shift, and the response with climate factors was mainly characterized by having consistent or opposite center of gravity changes with precipitation and potential evapotranspiration. ③ Human activities have been the dominant factor in the vegetation NDVI change, with 75.89 percent of the area positively impacted by human activities, and human activities in the southwest inhibiting the improvement of vegetation in the area. The impact of human activities on the unchanged land type area is increasing, most obviously in the farmland area, and the impact of human activities on the changed land type area is gradually decreasing in the area where the farmland becomes impervious. The vegetation in the area above 1300 m above sea level is degraded by the environment and human activities. The research results can provide scientific support for the implementation of ecological fine management and the formulation of corresponding ecological restoration and desertification control measures in the Maowusu Sandland. At the same time, it is expected to serve as a baseline for other studies on the evolution of vegetation in agro-pastoral zones.

Evaluating Land Surface Temperature Variation and Its Responses to Climate Change and Human Activities on the Southeastern Tibetan Plateau

ABSTRACTThe Yarlung Zangbo River Basin (YZRB), situated within the Qinghai‐Tibetan Plateau, has experienced significant alterations due to global warming and vegetation greening. This region serves as a critical indicator of the interplay between vegetation growth and climatic fluctuations, as evidenced by substantial changes in spatiotemporal land surface temperature (LST) over recent decades. In this research, we assessed the components of the water and energy cycles from 1980 to 2015 utilising the variable infiltration capacity (VIC) model to generate a continuous daily LST data over a 35‐year period. Subsequently, we analysed the variations in LST and identified the influence of environmental factors on temperature changes. Notably, while greening was observed, LST exhibited an upward trend. By differentiating the effects of climatic and anthropogenic factors on LST, we found that climate was the predominant influence, accounting for a contribution rate of 70.36% from 1980 to 1995. In contrast, human activities became the primary driver of LST changes, contributing 55% after 1995. Grasslands with moderate coverage demonstrated potential cooling effects. Among the various environmental factors examined, albedo exhibited a negative and delayed impact on LST, while temperature, precipitation and evapotranspiration were positively correlated with LST, displaying relatively synchronous variations. Additionally, soil moisture and the normalised difference vegetation index (NDVI) were identified as leading contributors to positive changes in LST. This study enhances the understanding of the mechanisms influencing LST and provides essential insights for socio‐economic development in areas with sensitive ecosystem.

Ecosystem services driving factors and ecological conservation pattern construction, Qinghai-Tibet Plateau

Given that the Qinghai-Tibet Plateau (QTP) is an important ecological security barrier in western China, changes in its ecosystem services (ESs) have received attention. Using multivariate datas and model simulations, we evaluated the ESs of the QTP, revealed their driving factors, and constructed ecological conservation pattern based on ecosystem services index (ESI) and ecosystem sensitivity (ES). The results showed that: (1) The overall ESs of the QTP from 2000 to 2020 were positive, with a spatial distribution characteristic of decreasing from southeast to northwest. Forest and grassland were the key land use types promoting ESs. (2) Natural factors had a stronger influence on ESs than socioeconomic factors, with precipitation, FVC (Fractional Vegetation Cover) and NPP (Net Primary Productivity) had the strongest explanatory power for ESs. (3) The proportion of the collaborative development area (CDA), consolidation and enhancement area (CEA) and priority protection area (PPA) to the total area of the QTP was 19%, 56% and 25% respectively. The CDA should follow the concept of natural restoration and green development. The CEA should optimize the management system and do a good job of environmental management in combination with artificial restoration. The PPA should strictly prevent disturbances caused by human activities and protect the originality of natural landscapes such as glaciers, snow and permafrost.

Novel oil-associated bacteria in Arctic seawater exposed to different nutrient biostimulation regimes.

The Arctic Ocean is an oligotrophic ecosystem facing escalating threats of oil spills as ship traffic increases owing to climate change-induced sea ice retreat. Biostimulation is an oil spill mitigation strategy that involves introducing bioavailable nutrients to enhance crude oil biodegradation by endemic oil-degrading microbes. For bioremediation to offer a viable response for future oil spill mitigation in extreme Arctic conditions, a better understanding of the effects of nutrient addition on Arctic marine microorganisms is needed. Controlled experiments tracking microbial populations revealed a significant decline in community diversity along with changes in microbial community composition. Notably, differential abundance analysis highlighted the significant enrichment of the unexpected genera Lacinutrix, Halarcobacter and Candidatus Pseudothioglobus. These groups are not normally associated with hydrocarbon biodegradation, despite closer inspection of genomes from closely related isolates confirming the potential for hydrocarbon metabolism. Co-occurrence analysis further revealed significant associations between these genera and well-known hydrocarbon-degrading bacteria, suggesting potential synergistic interactions during oil biodegradation. While these findings broaden our understanding of how biostimulation promotes enrichment of endemic hydrocarbon-degrading genera, further research is needed to fully assess the suitability of nutrient addition as a stand-alone oil spill mitigation strategy in this sensitive and remote polar marine ecosystem.

Impact of landscape pattern changes on ecosystem service values in the Fenhe River Basin in China

The evolution of landscape patterns affects the supply of ecosystem services. It is important to explore the relationship between them to improve regional ecological stability and maintain ecological health. The Fenhe River is the second largest tributary of the Yellow River and it represents a fragile ecological environment. Our study aimed to analyze the impact of changes in landscape patterns on the value of ecosystem services (ESV) in the Fenhe River Basin (FRB). Land use data from 2000, 2010, and 2020 were used to extract landscape pattern indices. Methods such as ecosystem service value accounting, sensitivity analysis, correlation analysis, and geodetector modeling were employed to investigate changes in landscape patterns and ESV, and to identify the driving forces and impacts of these changes on ESV. The results showed that (1) from 2000 to 2020, the dominant landscape in the FRB was cultivated land, followed by forestland and grassland, which together covered 92% of the total area. (2) Over the past 20 years, the extent of landscape fragmentation had continued to increase and ecological function had deteriorated. (3) ESV had continued to decrease. The ESV provided by the regulating service function was the largest, accounting for over 49% of the total ESV. Support service function was second, accounting for over 34%. (4) The total ESV had a strong negative correlation with Shannon's diversity index (SHDI) and Shannon's evenness index(SHEI), a significant positive correlation with Contagion index (CONTAG). (5)The Human Activity Index (HAI) had a significant impact on changes in ESV, with an influence value of 0.6192, and its synergistic effect with NDVI further enhances this impact, achieving a synergy score of 0.71. Additionally, natural factors such as topography and climate also had significant effects on ESV changes.This study provides an evidence-based theoretical reference to guide the enhancement of the natural environment of the FRB and maintain highquality development of regional landscapes.

Development and Application of an Environmental Vulnerability Index (EVI) for Identifying Priority Restoration Areas in the São Francisco River Basin, Brazil

The environmental vulnerability diagnosis of a river basin depends on a holistic analysis of its environmental aspects and degradation factors. Based on this diagnosis, the definition of priority areas where interventions for environmental recovery should be carried out is fundamental, since financial and natural resources are limited. In this study, we developed a methodology to assess these fragilities using an environmental vulnerability index (EVI) that combines physical and environmental indicators related to the natural sensitivity of ecosystems and their exposure to anthropogenic factors. The developed EVI was applied to the headwater region of the São Francisco River Basin (SFRB), Brazil. The proposed index was based on the AHP multicriteria analysis and was adapted to include four variables representative of the study area: Land Use Adequacy, Burned Area, Erosion Susceptibility, and quantitative water balance. The EVI analysis highlighted that the presence of easily erodible soils, associated with sloping areas and land use above their capacity, generate the most vulnerable areas in the headwaters of the SFRB. The highest EVI values are primarily linked to regions with shallow, easily erodible soils like Leptosols and Cambisols, found in steep areas predominantly used for pasture. In the SFBR, the greatest vulnerability was observed within a 5 km buffer around conservation units, covering approximately 32.4% of the total area. The results of this study indicate where resources should be applied for environmental preservation in the basin under study, directing the allocation of efforts to areas with lower resilience to maintain ecosystem services.

Diatom dynamics during the last six centuries in Lake Odensjön: a new varved sediment record from southern Sweden

AbstractVarved lake sediments offer valuable insight into past environmental conditions with high temporal resolution and precise chronological control. A combination of diatom and geochemical analyses of the recently deposited sediments of Odensjön, a small dimictic lake in southern Sweden, shows alternating light and dark laminae composed of greater amounts of biogenic silica and organic matter, respectively. As confirmed by independent radiometric dating and Pb pollution data, and supported by scanning electron microscopy of individual laminae, these features represent ongoing deposition of biogenic varves. Corresponding diatom and geochemical data obtained from a 92-cm long freeze core provide evidence of substantial lake-ecosystem dynamics during the last six centuries, related mainly to variations in light penetration and wind shear driven by human-induced changes in catchment vegetation. The diatom assemblage of Odensjön’s varved sediments is dominated by planktonic species, primarily Asterionella formosa, Fragilaria saxoplanktonica and Discostella lacuskarluki during periods of forest cover, while increased catchment openness from the mid-1500s to the late 1800s led to increased abundance of Lindavia comensis. Long-term variations in climate and land use, mediated through changing length of the ice-cover season and nutrient input, respectively, probably contributed to the observed trends, as well as to variations in the appearance and visibility of the varve record across the sampled sediment sequence. Odensjön represents the southernmost varved sediment record in Fennoscandia documented to date, offering potential to study the effects of various types of external forcing on its sensitive lacustrine ecosystem since the Late Weichselian deglaciation. In the present study, we investigated the possibility of assessing the local impacts of two major, historically documented volcanic events, Laki 1783–84 and Tambora 1815, which are known to have affected European societies. Although the mildly alkaline waters of the lake are well buffered and hence relatively resilient to volcanic acid deposition, a minor response to the Laki eruption may be recorded in the diatom stratigraphy.

Fish mortality in the Amazonian drought of 2023: the role of experimental biology in our response to climate change.

Higher temperatures exacerbate drought conditions by increasing evaporation rates, reducing soil moisture and altering precipitation patterns. As global temperatures rise as a result of climate change, these effects intensify, leading to more frequent and severe droughts. This link between higher temperatures and drought is particularly evident in sensitive ecosystems like the Amazon rainforest, where reduced rainfall and higher evaporation rates result in significantly lower water levels, threatening biodiversity and human livelihoods. As an example, the serious drought experienced in the Amazon basin in 2023 resulted in a significant decline in fish populations. Elevated water temperatures, reaching up to 38°C, led to mass mortality events, because these temperatures surpass the thermal tolerance of many Amazonian fish species. We know this because our group has collected data on critical thermal maxima (CTmax) for various fish species over multiple years. Additionally, warmer waters can cause hypoxia, further exacerbating fish mortality. Thus, even Amazon fish species, which have relatively high thermal tolerance, are being impacted by climate change. The Amazon drought experienced in 2023 underscores the urgent need for climate action to mitigate the devastating effects on Amazonian biodiversity. The fact that we have been able to link fish mortality events to data on the thermal tolerance of fishes emphasizes the important role of experimental biology in elucidating the mechanisms behind these events, a link that we aim to highlight in this Perspective.

Methylmercury in commercial fish species from the Erhai Lake Basin, Southwest China: concentrations, health risk assessment, and implications for future monitoring.

Erhai Lake, a highland lake situated in Southwest China, provides critical aquatic protein sources for the local community, and its preservation is vital due to the sensitivity of alpine freshwater ecosystems to disturbance. However, there is a lack of research on the contamination status of methylmercury (MeHg) in aquatic organisms of the Erhai Lake Basin. MeHg concentrations in important commercial fish species from the Erhai Lake were examined, and the potential health risks associated with human consumption were assessed. Our results showed significant inter-species differences in fish muscle MeHg: the carnivorous S. asotus exhibited the highest level (303ng/g; ww), while that of the detritivorous R. ocellatus was the lowest (3.86ng/g). Moreover, MeHg concentrations in P. fulvidraco and C. auratus collected from the Luoshi River (a major tributary of Erhai Lake) were significantly higher compared to those from the Erhai Lake, indicating possible river-based input of MeHg into the Erhai Lake. Additionally, our study revealed a significant positive correlation between the MeHg levels and the length as well as weight of the examined fish species. All the fish species analyzed in our study had MeHg concentrations within the limits of China's food safety standard. Nevertheless, a relatively low consumption quantity of 16g per day of certain species (i.e., S. asotus) may still pose potential health risks especially for children. The present study provides baseline data for MeHg monitoring and risk assessment in the Erhai Lake Basin, and warrants continued monitoring and source investigation.

Ultraviolet-induced melanisation in lichens: physiological traits and transcriptome profile.

Lichens are important components of high-latitude boreal and Arctic habitats. While stress tolerant, they are among the most sensitive ecosystem components to climate change, in particular, an increase in ultraviolet light (UV) arising from polar ozone depletion and deforestation. This study is the first to explore the effects of UV-B on gene expression in lichens to predict metabolic pathways involved in tolerance. Using transcriptome profiling and bioinformatic analyses, here we studied the effects of UV-B on gene expression in lichens using Lobaria pulmonaria (L.) Hoff. as a model species. UV-B exposure causes significant browning of the upper cortex of the thallus, which correlates to an increased expression of biosynthetic gene clusters involved in the synthesis of eu- and allomelanins and melanin precursors. Based on transcriptome analyses, we suggest that the biosynthesis of melanins and other secondary metabolites, such as naphthalene derivates, tropolones, anthraquinones, and xanthones, is a trade-off that lichens pay to protect essential metabolic processes such as photosynthesis and respiration. Expression profiles of general stress-associated genes, in particular, related to reactive oxygen species scavenging, protection of proteins, and DNA repair, clearly indicate that the mycobiont is the more UV-B-responsive and susceptible partner in lichen symbiosis. Our findings demonstrate that UV-B stress activates an intricate gene network involved in tolerance mechanisms of lichen symbionts. Knowledge obtained here may enable the prediction of likely effects on lichen biodiversity caused by climate change and pollution.

Progressive melting of surface water and unequal discharge of different DOM components profoundly perturb soil biochemical cycling

Freeze-thaw (FT) events profoundly perturb the biochemical processes of soil and water in mid- and high-latitude regions, especially the riparian zones that are often recognized as the hotspots of soil-water interactions and thus one of the most sensitive ecosystems to future climate change. However, it remains largely unknown how the heterogeneously composed and progressively discharged meltwater affect the biochemical cycling of the neighbor soil. In this study, stream water from a valley in the Chinese Loess Plateau was frozen at –10°C for 12 hours, and the meltwater (at +10°C) progressively discharged at three stages (T1 ∼ T3) was respectively added to rewet the soil collected from the same stream bed (Soil+T1 ∼ Soil+T3). Our results show that: (1) Approximately 65% of the total dissolved organic carbon and 53% of the total NO3--N were preferentially discharged at the first stage T1, with enrichment ratios of 1.60 ∼ 1.94. (2) The dissolved organic matter discharged at T1 was noticeably more biodegradable with significantly lower SUVA254 but higher HIX, and also predominated with humic-like, dissolved microbial metabolite-like, and fulvic acid-like components. (3) After added to the soil, the meltwater discharged at T1 (e.g., Soil+T1) significantly accelerated the mineralization of soil organic carbon with 2.4 ∼ 8.07-folded k factor after fitted into the first-order kinetics equation, triggering 125 ∼ 152% more total CO2 emissions. Adding T1 also promoted significantly more accumulation of soil microbial biomass carbon after 15 days of incubation, especially on the FT soil. Overall, the preferential discharge of the nutrient-enriched meltwater with more biodegradable DOM components at the initial melting stage significantly promoted the microbial growth and respiratory activities in the recipient soil, and triggered sizable CO2 emission pulses. This reveals a common but long-ignored phenomenon in cold riparian zones, where progressive freeze-thaw can partition and thus shift the DOM compositions in stream water over melting time, and in turn profoundly perturb the biochemical cycles of the neighbor soil body.

Application of the Biological Toxicity Effect Ratio (BER) Method for Advancing Water Quality Criteria Derivation.

Water quality criteria (WQC) serve as a scientific foundation for pollutant risk assessment and control in aquatic ecosystems. The development of regionally differentiated WQC tailored to specific regional characteristics has become an emerging trend. However, the current WQC is constrained by a lack of regional species toxicity data. To address these limitations, this study proposes the biological toxicity effect ratio (BER) method, which indirectly reflects the toxicity sensitivity of the overall aquatic ecosystem through the toxicity information on a limited number of species, enabling rapid WQC prediction. Using the established WQC in China and the USA as a case study, we combined mathematical derivation and data validation to evaluate the BER method. Among various species-taxon groups of freshwater organisms, planktonic crustaceans demonstrated the highest predictive accuracy. Our analysis further revealed that species toxicity sensitivity and regional variability jointly influence the prediction accuracy. Regardless of the evaluation indexes, planktonic crustaceans emerged as the most suitable species-taxon group for the BER method. Additionally, the BER method is particularly applicable to pollutants with conserved mechanisms across species. This study systematically explores the feasibility of using the BER method and offers new insights for deriving regionally differentiated WQC.

Shallow soils in dryland ecosystems: Drivers of C accumulation and land management implications

Shallow soils (i.e., Lithic Entisols) cover about 20% of Brazilian drylands. Inherent soil characteristics (i.e., shallow depths) and water scarcity restrict plant growth and carbon (C) inputs in these soils. In such a sensitive ecosystem, sustainable land management options are key to promoting socio-economic development and ensuring food security. Here, we use a dataset of 50 Lithic Entisol profiles distributed within the Brazilian drylands (northeastern region) to: (i) investigate the effects of land-use (cropland, grassland, and native forest), climate (semi-arid and dry sub-humid), and slope classes (0–3%, 3–8%, 8–20% and 20–45%) on soil C accumulation; and (ii) evaluate how understanding multiple drivers C accumulation can support the identification of sustainable land management options. The results suggested that land use does not affect C stock and C/N ratio, nevertheless, they were affected by climate. Under dry sub-humid climate conditions, C stocks were 41.7 Mg ha−1, 44% higher than under semi-arid climate (28.9 Mg ha−1), a result that reflects the effect of higher biomass production in wetter environments. Under slopes of 20–45%, C stocks were 54.3 Mg ha−1, 172% higher than under slopes of 0–3% (19.9 Mg ha−1), because of the higher altitudes and wetter conditions under steeper slopes. Our results showed that areas under lower slopes have lower C stocks and lower aridity index. These drier conditions reduce the productive potential of annual crops and grasslands but enable the cultivation of high-yielding Crassulacean Acid Metabolism (CAM) crops, such as Agave spp, which have a high potential for biofuel production. Furthermore, the possibility of reintroducing organic residues from ethanol production can promote an increase in C stocks, contributing to climate change mitigation. Ultimately, our study provides insights from a holistic view of SOC accumulation drivers, supporting land use planning of highly sensitive environments in tropical drylands around the world.

Distribution Characteristics and Ecological Risk Assessment of Heavy Metals in Roadside Soil of Important National Highways on the Qinghai-Xizang Plateau

To clarify the impact of transportation on the sensitive and fragile ecosystems of the Qinghai Tibet Plateau and major ecological safety barrier functions, soil samples within 0-25 m on the roadside were collected from sections of national highways such as G214, G213, G345, G109, G316, and G317, and the contents of six heavy metals were analyzed. Then, the degree of heavy metal pollution and the risk of ecological hazards were evaluated using the single-factor pollution index method （Pi）, Nemero comprehensive index method （PN）, and potential ecological risk index method （RI）. The results showed that the heavy metal contents of As, Cd, Hg, Ni, Pb, and Zn in the soil of important transportation national roads on the Qinghai Tibet Plateau ranged from 5.65 to 176.00, 0.04 to 0.27, 0.01 to 0.14, 9.52 to 113.00, 9.16 to 54.50, and 24.70 to 109.00 mg·kg-1, respectively, showing high variability. In some sections of the soil, the values of the elements As, Cd, and Hg were higher than the local soil background values. The single-factor pollution index of heavy metals in roadside soil was Pi （As） > Pi （Hg） > Pi（Cd） > Pi （Pb） > Pi （Ni） > Pi （Zn）. The Nemero comprehensive pollution index ranged from 0.41 to 9.20, with an average value of 1.53, indicating clean and mild pollution. Some areas showed a moderate or severe pollution. The average potential ecological risk index of the research section was 106.2, and the soil was generally in a state of no pollution and light pollution. Only two road sections had soil heavy metal enrichment reaching moderate and strong ecological hazards. The comprehensive potential risk of the G213a road section indicated moderate to severe ecological risk, mainly contributed by Hg, As, and Cd. The comprehensive pollution risk of the G317 road section indicated mild to moderate ecological risk, mainly contributed by Hg and Cd. The heavy metal content in the soil of the Qinghai Tibet Plateau road area was not significantly correlated with the roadside distance and soil depth but was significantly positively correlated with the annual average temperature （P < 0.05）. In all, there was a trend of heavy metal input into the soil environment in areas with intense human activities and high traffic flow during road construction on the Qinghai Tibet Plateau.

Microplastics in bulk atmospheric deposition along the coastal region of Victoria Land, Antarctica

The increasing global concern over microplastic pollution has driven a surge in research efforts aimed at detecting microplastics across various ecosystems. Airborne microplastics (MPs) have been identified in remote environments worldwide, including Antarctica. However, data on bulk atmospheric deposition remain scarce. From January to December 2020, atmospheric deposition was directly collected using passive samplers placed in eight sites across Victoria Land. Using Raman Microspectroscopy, MPs were identified in six out of the seven samples collected (one sample was lost due to the extreme weather conditions). The average daily MP deposition for Victoria Land was 1.7 ± 1.1 MPs m−2 d−1, with values ranging from 0.76 to 3.44 MPs m−2 d−1. The majority (53 %) of MPs found in the atmospheric deposition were in the size class of 5–10 μm, and the main shape of MPs was fragments (95 %). The predominant plastic type was polypropylene (31 %), followed by polyethylene (19 %) and polycarbonate (12 %). Polystyrene, polyester, styrene and polyethylene terephthalate each accounted for ~6 %. Microplastics identified in the coastal sites may have local origins, potentially associated with scientific activities at research stations. Conversely, a backward trajectories analysis suggested a potential contribution of atmospheric transport to microplastic deposition at Larsen Glacier and Tourmaline Plateau, the two most remote sites of the study area, where the highest MP concentrations were detected. Our findings present the first evidence of microplastics in the Antarctic atmospheric deposition directly collected via passive samplers, highlighting the need for continued monitoring and research to assess the environmental impact of MPs, particularly in sensitive and remote ecosystems like Antarctica.

The design and development of EcoBiomes: Multi-species synthetic microbial consortia inspired by natural desert microbiome to enhance the resilience of climate-sensitive ecosystems

Synthetic microbial communities, which simplify the complexity of natural ecosystems while retaining their key features, are gaining momentum in engineering and biotechnology applications. One potential application is the development of bioinoculants, offering an eco-friendly, sustainable solution to promote plant growth and increase resilience to abiotic stresses amidst climate change. A potential source for stress-tolerant microbes is those associated with desert plants, evolved and shaped by selective pressures to promote host health under harsh environmental conditions. In our research, we aim to design and develop synthetic microbial consortia inspired by the natural microbiota of four desert plants native to the Arabian Peninsula, inferred from our previous work identifying the structure and predicting the function of these microbial communities using high throughput eDNA barcoding. To obtain culturable microbes that are manageable and traceable yet still representative of natural microbial communities, we combined multiple experimental protocols coupled with compatibility and synergy assessments, along with in planta testing. We isolated a total of 75 bacteria and conducted detailed biological evaluations, revealing that an overwhelming majority (84 %) of all isolates produced indole acetic acid (IAA), with 73 % capable of solubilizing phosphate, 60 % producing siderophores, 47 % forming biofilms, and 35 % producing ACC deaminase, all contributing to plant growth and stress tolerance. We constructed four synthetic microbial consortia, named EcoBiomes, consisting of synergistic combinations of multiple species that can co-exist without significant antagonism. Our preliminary data indicate that EcoBiomes enhance the resilience of heterologous host plants under simulated environmental stresses, including drought, heat, and salinity. EcoBiomes offer a unique, sustainable, and eco-friendly solution to mitigate the impact of climate change on sensitive ecosystems, ultimately affecting global food security.

Permafrost degradation is a legal matter

New topical problems of permafrost degradation are identified in the context of a new sphere of legal regulation, primarily in the Arctic countries, with an emphasis on the legal problems of Russia. The object of research in the article consists of various legal consequences of the said phenomenon, manifested on very sensitive ecosystems, human, industrial, transport land spaces, that is, outside the maritime spaces. The study is based primarily on an integrated legal approach and appropriate legal instruments to address the emerging economic and legal consequences of the degradation of permafrost. This approach makes it possible to substantiate the recognition as an independent object of regulation of the phenomenon of permafrost and the need to develop its own legal conceptual framework. This approach is justified at both the national and international levels, as well as in the analysis of specialized and modified law institutions of the Arctic States. The article applies the advantage of the comparative legal method of studying the legislation of the Arctic states, as well as interdisciplinary and intersectoral methods of legal research. As a result, the need for a new independent scientific and applied integrated study of the legal problems of the degradation of permafrost can be considered. One of the conclusions of the work included the need to create international specialized legislation in the form of a model act. Research in the proposed format may determine the structure and content of future international legal regulation and the institutions that support it.