Sensitive Detection Of Kanamycin Research Articles

Ratiometric fluorescence platform for the ultrasensitive detection of kanamycin based on split aptamer co-recognition triggers Mg2+-DNAzyme-driven DNA walker systems

In this work, a novel 3D-DNA walker signal amplification strategy was designed to construct a fluorescent aptasensor for the detection of kanamycin (KAN). The aptasensor utilizes split aptamers for the synergistic recognition of KAN. The presence of KAN induces the split aptamers recombination to form the Mg2+-DNAzyme structure, which is activated by Mg2+ to drive the 3D-DNA walker process for cascading signal amplification. Employing gold nanoflowers (AuNFs) as walking substrate material increases the local DNA concentration to enhance the walker efficiency. The prepared fluorescent aptasensor achieved efficient and sensitive detection of KAN with satisfactory results in the concentration range of 1 × 10−8 - 1 × 10−3 μg/kg and the detection limit of 5.63 fg/kg. Meanwhile, the designed fluorescent aptasensor exhibited favorable specificity, anti-interference, storage stability and reproducibility, and verified the feasibility of its application in milk samples. The present work provides an effective tool for the regulation of KAN contamination in animal-derived foods with promising prospects.

A smartphone-based nanoenzyme-modulated aptasensor using an infrared camera for rapid detection of kanamycin

The detection of antibiotics in water is crucial to ensure the safety of drinking water and prevent the spread of antibiotic resistance in the environment. In this study, we present a novel strategy for the selective and sensitive detection of kanamycin (KAN) in water by combining infrared thermography with aptamer detection technology. The aptamers specific to KAN were immobilized on the surface of gold nanoparticles (AuNPs), thereby enhancing the catalytic activity of AuNPs in the 3,3′,5,5′-tetramethylbenzidine (TMB)-hydrogen peroxide system to produce oxidized TMB with photothermal conversion capability. KAN competitively binds to the aptamer and modulates the catalytic capacity of the AuNPs surface, resulting in different photothermal conversion capacities of the final solution. Under near-infrared laser irradiation, the target KAN concentration is converted into a measurable thermal signal. To fabricate a portable sensing setup, we integrated the device with a smartphone. The aptasensor can selectively detect KAN with a limit of detection of 1.55 ng·mL−1 and a limit of quantification of 13.62 ng·mL−1. The detection time of our sensor is real time with a reaction time of 30 min. It demonstrates excellent stability and recoveries ranging from 96.9 % to 106.1 % for practical water samples. This portable aptasensor offers rapid detection, simplicity, and affordability, enabling real-time analysis of antibiotic residues in water.

Enhanced-sensitivity and rapid-response for the detection of kanamycin based on ethanol-assisted AIEE effect in silver-doped gold nanoclusters

In this study, a novel solvent-assisted strategy was developed for the rapid and sensitive detection of kanamycin (KAN) based on glutathione-capped silver-doped gold nanoclusters (AuAg NCs). Ethanol solvent played a key role in disrupting the hydration shell of metal(I)-thiolate complexes in AuAg NCs. KAN, as a specific linker between AuAg NCs through electrostatic interaction and metal-amine coordination, induced the formation of large aggregates and thus triggered prolonged photoluminescence lifetime as well as strong luminescence enhancement by restricting molecular motions in AuAg NCs. The KAN-stimulated turn-on principle allows AuAg NCs to exhibit 4-fold sensitivity in 40% ethanol/water (V/V) mixed solution compared to that in water. The improved aggregation-induced-emission enhancement (AIEE) feature endows the AuAg NCs with selective recognition for KAN and realizes rapid detection in a short response time of 60 s with a low detection limit of 8.6 nM. By integrating AuAg NCs with a Pasteur pipette, a facile sample-to-answer test kit for on-site determination with low cost and high efficiency was constructed, offering potential applications in the timely analysis of contaminants in environmental emergencies.

A ZnIn2S4@ReS2/AgInS2-based photoelectrochemical aptasensor for the ultrasensitive detection of kanamycin.

An ultrasensitive photoelectrochemical (PEC) aptasensor was originally designed by using ZnIn2S4/ReS2 as a photoactive material and AgInS2 as a signal amplifier. The signal amplifier AgInS2 was incubated on the terminal of H-DNA (immobilized on the ZnIn2S4/ReS2/FTO surface), leading to an enhanced photocurrent response. Then, due to the introduction of DNA2, the formation of a double-stranded structure caused AgInS2 to keep away from the electrode surface, and the photocurrent was reduced. In the presence of kanamycin, DNA2 was released from the system due to the competition relationship, and a restored photocurrent response was obtained. The combination of ZnIn2S4/ReS2 and AgInS2 accelerated the electron transfer and enhanced the separation efficiency of photogenerated electron-hole pairs, resulting in an improved performance of the PEC aptasensor, which was capable of accurate and sensitive detection of kanamycin in actual samples.

A label-free and enzyme-free fluorescent aptasensor for amplified detection of kanamycin in milk sample based on target-triggered catalytic hairpin assembly

Kanamycin residues in foods can cause serious adverse reactions in human body, and the accurate and sensitive detection of kanamycin is of great significance to ensure human health. In this work, we constructed a label-free and enzyme-free fluorescent aptasensor for quantifying kanamycin in milk based on target-triggered catalytic hairpin assembly. First, we designed an aptamer probe that contained an aptamer unit for recognizing kanamycin, a trigger unit for triggering catalytic hairpin assembly and a suppression unit for ensuring the stability of aptamer probe. In the initial state, the trigger unit was closed by suppression unit and inactive. After the aptamer unit recognized and bound to kanamycin, a free trigger unit was released to trigger the cycle hybridization reactions between hairpin probe 1 and hairpin probe 2, generating G-rich complexes and realizing enzyme-free amplification detection. Then, N-methylmesoporphyrin IX as signal molecules were combined with G-rich complexes to generate a significant fluorescent signal, which realized label-free detection. Thus, the aptasensor provided a well sensitivity for kanamycin with a detection limit of 0.26 ng/mL. And it possessed well selectivity and could distinguish kanamycin from its analogues, which stemmed from the specific recognition between kanamycin and aptamer. The aptasensor had great potential application value in the field of ensuring food safety.

Ultrasensitive all-solid-state electrochemiluminescence platform for kanamycin detection based on the pore confinement effect of 0D g-C3N4 quantum dots/3D graphene hydrogel

Quests for stable and highly efficient electrochemiluminescence (ECL) emitters and a full understanding of the relation between the ECL emitter structure and its properties are a continual and active theme in the fields of ECL. In this work, an intense and stable ECL platform was achieved by encapsulating 0D graphite carbon nitride quantum dots (g-C3N4 QDs) into 3D graphene hydrogel via a facile hydrothermal approach. The architectures of the as-fabricated 0D g-C3N4 QDs/3D graphene hydrogel nanocomposites showed 4.3-fold ECL intensity relative to that of pure g-C3N4. This phenomenon was caused by a pore confinement effect, which could increase the electrochemical reaction efficiency because the pore channels could promote electron transfer and mass transport. The confinement effect could also localise a very large luminophore near the electrode surface, resulting in enhanced ECL emission. Moreover, the as-fabricated architecture ensured the stability of the g-C3N4 QDs in the ECL field due to the increased dispersibility. On the basis of the excellent ECL performances, a novel ECL biosensor was fabricated for highly selective and sensitive detection of kanamycin (KAN) with the assistance of an aptamer. The proposed ECL sensor can quantify KAN from 1 pM to 50 nM with a limit of detection of 0.33 pM. Such work offers an efficient design idea for achieving a high-performance ECL platform and provides new insight into developing and applying a g-C3N4-based ECL system.

Visual detection of kanamycin with DNA-functionalized gold nanoparticles probe in aptamer-based strip biosensor

Kanamycin has been widely used to treat human and animal diseases. The excessive use of kanamycin causes its accumulation in animal-derived foods, and eventually threats human health. In the present study, we develop a lateral flow strip biosensor for fast and sensitive detection of kanamycin. The strip biosensor combines the easy separation of magnetic microspheres (MMS) with target-mediated chain displacement of single-stranded DNA and the capture of the visible DNA-functionalized gold nanoparticles (AuNPs) probe. The presence of kanamycin can competitively bind to the aptamer and release cDNA to the supernatant. The concentration of free cDNA, which is the direct target of the strip, is proportional to the concentration of kanamycin. The capture of DNA-functionalized AuNPs on the test zone of the strip through cDNA-induced hybridization provides a visual detection signal. The assay can be completed within 20 min. The visual detection limit by naked eyes of the strip is 50 nM. A linear detection range of 5–500 nM is derived for quantitative determination, with the detection limit of 4.96 nM (S/N = 3). This lateral flow strip biosensor can quickly and sensitively detect kanamycin in different food samples, which holds great application potential in medicine and daily life.

Label-free Kanamycin sensor development based on CuO[sbnd]NiO hollow-spheres: Food samples analyses

Kanamycin (KAN) is commonly used as an amino-glycoside antibiotic and enduring KAN in animal derivative food causes intensive adverse effects in human health. Here, at first copper oxide co-doped nickel oxide hollow-spheres (CuONiO HSs) were synthesized using a facile hydrothermal method (HTM) in an alkaline phase and then characterized by various conventional methods such as Fourier-transform infrared spectroscopy (FTIR), UV–visible spectroscopy (UV–vis.), Field Emission Scanning Electron Microscopy (FESEM), Energy-dispersive X-ray spectroscopy (XEDS), X-ray photoelectron spectroscopy (XPS), and powder X-ray diffraction (XRD) to find out the nature of materials as binding energy, band-gap energy, morphology, crystallinity, functional groups, and elemental analyses. A selective and label-free KAN sensor was developed with the modification of slight coating of HSs onto a glassy carbon electrode (GCE) using nafion (Nf) coating binder at room conditions. Improved electrochemical performances of sensitivity, linear dynamic range (LDR), and long-term stability of selective KAN were achieved by reliable electrochemical technique. Based on signal to noise ratio 3, sensitivity and limit of detection (LOD) of sensor were calculated as 1.9 × 10−4 μAμM−1 cm−2 and 0.5 ± 0.02 nM respectively. The hydrothermally synthesized CuONiO HSs is significant advancement in fabricating with Nf/GCE decorated sensor probe in favor of enzyme-free detection of chemicals in biological system. In conclusion, the proposed sensors can be applied effectively for the selective and sensitive detection of KAN in food samples with satisfactory and acceptable results.

Design of an aptamer – based fluorescence displacement biosensor for selective and sensitive detection of kanamycin in aqueous samples

A label-free detection method for kanamycin A using an aptamer-based displacement biosensor has been developed.