Media has a impactful role for the spread of wellness, which was essential to attract public awareness. Media can facilitate health communication which targetted many stakeholders including governments and public citizen. Anemia is an individual contion which the number of haemoglobin concentration is lower than normal value. This study was carried out in Menganti Senior High School, East Java, Indonesia. Anemia comics were distributed to female student in this school. A total of 75 students were participated in this study. Every student were given pretest and posttest to assess their knowledge about anemia, including the clinical manifestation of anemia, diagnosis of anemia, prevention of anemia, and also long impact of anemia. Anemia comics can be used as a learning medium for the prevention of anemia which targeted adolescent girls. This study shows the difference significant result between pretest and posstest of targeted group after get the intervention.