Semisolid Preparations Research Articles

Formulation of Ethanol Extract Cream of Sikkam Leaves (Bischofia javanica Blume) as an Antifungal Against Candida albicans

Skin health issues in Indonesia, including those caused by fungal infections such as (Candida albicans), can lead to various conditions like candidiasis, scabies, tungiasis, and leprosy. This study aims to evaluate the potential of sikkam leaf extract in developing an antifungal cream to combat (Candida albicans). Fungi are eukaryotic organisms that cannot photosynthesize and reproduce through branching filaments composed of hyphae. Fungi can thrive in diverse environments such as water, soil, and decaying organic matter. A cream is a semi-solid emulsion preparation in which one or more medicinal substances are dissolved or dispersed within a base. This formulation determines the cream’s effectiveness, including the optimal ethanol extract concentration needed for antifungal activity. Extraction of sikkam leaves was conducted using maceration with 70% ethanol, and creams were formulated with extract concentrations of 5%, 10%, and 15%. Data were analyzed using the Kruskal-Wallis test. The study results showed that sikkam leaf-based creams meet quality standards, including organoleptic properties, homogeneity, pH, spreadability, and physical stability. Effectiveness testing indicated that creams with 5%, 10%, and 15% concentrations produced inhibition zones against (Candida albicans) with average diameters of 6.3 mm, 9 mm, and 15.3 mm, respectively. The higher the extract concentration, the stronger the inhibition of fungal growth. The cream with a 15% concentration proved to be the most effective, with inhibition classified as strong. Thus, sikkam leaf extract shows potential as a key ingredient for antifungal cream formulations.

Retinol semisolid preparations in cosmetics: transcutaneous permeation mechanism and behaviour

Retinol is widely used to treat skin ageing because of its effect on cell differentiation, proliferation and apoptosis. However, its potential benefits appear to be limited by its skin permeability. Herein, we investigated the transcutaneous behavior of retinol in semisolid cosmetics, in both in vitro and in vivo experiments. In vitro experiments used the modified Franz diffusion cell combined with Raman spectroscopy. In in vivo experiments, the content of retinol in rat skin and plasma was detected with HPLC. Retinol in semisolid cosmetics was mainly concentrated in the stratum corneum in the skin of the three animal models tested, and in any case did not cross the skin barrier after a 24 h dermatologic topical treatment in Franz diffusion cells tests. Similar results were obtained in live mice and rats, where retinol did not cross the skin barrier and did not enter the blood circulation. Raman spectroscopy was used to test the penetration depth of retinol in skin, which reached 16 μm out of 34 μm in pig skin, whereas the skin of mouse and rat showed too strong bakground interference. To explore epidermal transport mechanism and intradermal residence, skin transcriptomics was performed in rats, which identified 126 genes upregulated related to retinol transport and metabolism, relevant to the search terms “retinoid metabolic process” and “transporter activity”. The identity of these upregulated genes suggests that the mechanism of retinol action is linked to epidermis, skin, tissue and epithelium development.

Retinol and Oligopeptide-Loaded Lipid Nanocarriers as Effective Raw Material in Anti-Acne and Anti-Aging Therapies

The use of lipid nanocarriers as components of cosmetic formulations may provide an opportunity to fully exploit the beneficial properties of pentapeptide-18 and retinol while reducing the undesirable effects that occur during retinoid therapy. This study aimed to evaluate the effectiveness of semi-solid formulations enriched with retinol and oligopeptide-loaded lipid nanocarriers. Solid lipid nanoparticles were produced using a high-shear homogenization method. The work included physicochemical characterization of the cosmetic products, and evaluation of their stability as well as their efficacy. The resulting semi-solid preparations were determined to be stable regardless of their storage temperature. No effect of the presence of lipid nanoparticles on the shelf-life stability of the cosmetic products was observed. A temperature of 25 °C was considered the recommended storage temperature for the tested semi-solid formulations. Beneficial effects of the cosmetic products were proven (in vivo study on volunteers), i.e., a significant reduction in the level of sebum secretion (anti-acne therapy) and a decrease in the number of facial wrinkles (anti-aging therapy). In addition, the protective properties of the lipid nanoparticles themselves against the skin were confirmed, reducing the irritating effect of retinol that is usually the case with classic retinoid therapies.

Characterization of Structure-Surface Correlations in Ointments Using Surface Tensiometry within the Concept of an Integrated Analytical Approach.

In recent years, integrated data analysis has proven to be a valuable method for investigating complex systems, whether artificial or natural. The integrated analytical approach allows the simultaneous integration of data acquired from different analytical techniques employed for the same sample at the same time, leading to an expansion of the amount of information available. Surface tensiometry is a technique that was recently introduced in the Integrated Analytical Approach for investigating pharmaceutical dosage forms for topical application. Therefore, studies of rheological characterization, release, and skin permeation can be integrated with surface tensiometry measurements to develop chemical and rheological-surface correlation models, providing a new method for the quality control and process control of semisolid preparations. In this context, the aim of this research is to validate the utility of surface tensiometry measurements in the Integrated Analytical Approach and utilize these data to gain insights into the structure-surface correlation. The preparations chosen for this study were a PEG-gel, a lipogel, and an O/W cream containing carnitine as a model drug. The formulations were characterized using rheological measurements and evaluated for their carnitine release performance. Furthermore, surface tensiometry measurements were performed to rapidly and noninvasively assess the influence of carnitine on the surface properties of the semisolid preparations investigated. Our work demonstrated a close correlation between surface energy and structural data, showing the importance of surface tensiometry contribution in the noninvasive and rapid evaluation of the presence of carnitine in semisolid formulations.

Choorna Kalpana: Bridging Ayurvedic Traditions with Modern Size Reduction Techniques for Optimal Therapeutic Outcomes

Choorna Kalpana, a fine powder derived from completely dry drugs, is a fundamental preparation in Ayurveda. Recognized for its stability and ease of absorption, Choorna is used independently to treat various diseases, as an adjuvant with other medicines, and in secondary preparations like Avaleha (medicated semisolid preparation), Vati (tablets), and Sneha Kalpana (oil and ghee preparations). The preparation involves meticulous processes of washing, drying, pounding, and sieving drugs to ensure homogeneity. Modern practices in Choorna Kalpana incorporate size reduction techniques such as cutting, compression, impact, and attrition, which enhance its efficiency and therapeutic potential. Factors influencing size reduction, including hardness, toughness, abrasiveness, stickiness, softening temperature, and moisture content, are critical for achieving desired particle sizes. Size separation and mixing techniques are also essential in ensuring the uniformity and efficacy of Choorna. This paper explores the traditional and modern methodologies of Choorna preparation, highlighting its significance in Ayurvedic medicine.

Developement and Stability Evaluation of Natural Topical Formulations Containing Pinus sibirica Essential Oil

Abstract Pinus sibirica (P. sibirica) is used in folk medicine for centuries in treatment of various diseases due to its anti-inflammatory, antioxidant, and antimicrobial activity. The present study was focused on formulating semi-solid topical cream and gel preparations with P. sibirica essential oil (EOPS) and on evaluation of their stability during three months of storage. After preparation of semi-solid formulations (cream and gel), we followed parameters such as organoleptic characteristics, pH values, electrical conductivity, phase separation, washability, swelling index and microbiological contamination. Tests for physical and microbiological stability were performed considering different storage conditions at temperature (4±2° C, 25±2°C and 40±2°C) for 12 weeks. All test were performed at sampling time points of 7th, 30th, 60th, 90th day after storage. Cream with EOPS was white colour, it is homogenous with pleasant odor characteristic to used EOPS while gel with EOPS was transparent, it is homogenous with specific odor. After application to skin, EOPS cream was leaving non-greasy film while gel with EOPS was leaving sticky film and cooling effect. In all tested samples, we observed no phase separation and pH values were optimal for skin application. During three months of storage at different conditions, all tested formulations were stable and addition of EOPS did not cause any changes in followed parameters. Both cream and gel formulation remained stable since we did not notice any significant changes in all followed parameters during storage at different temperatures. Addition of 0.5% of EOPS did not change visual, sensory and microbial properties of formulations.

Technological and Physical-Chemical Evaluation of Cotton Gauzes Impregnated with Semisolid Preparations for Wound Healing.

Chronic wounds are marked by an extended healing period during which damaged tissues fail to undergo orderly and timely repair. Examples of chronic wounds encompass venous ulcers, pressure ulcers, and diabetic foot ulcers. The process of wound healing is complex and dynamic, relying on the interplay and response among various cells and mediators. In this study, four marketed wound dressing products based on cotton gauzes impregnated with different semisolid products (namely Betadine® 10%, Connettivina® Bio Plus Fitostimoline® Plus, and Non-Ad® gauzes) have been characterized for their physicochemical properties and ex vivo behaviors. More in detail, the pH and rheological features of semisolid formulations impregnating the gauzes were analyzed along with their ability to adhere to the gauzes. The most promising ones were selected and compared in ex vivo experiments on fresh pig skin. The pH measurements showed an acidic environment for all the tested solutions, albeit with variations in mean values, ranging from 2.66 to 4.50. The outcomes of rheological studies demonstrated that all the semisolid preparations impregnating the gauzes exhibited a pseudoplastic behavior, with significant differences in the pseudoplasticity index across the preparations, which is likely to influence their ability to adhere to the gauze. A rheological study in oscillatory mode revealed rheological behavior typical of a viscous solution only for the cream impregnating non-paraffin gauzes. The other products exhibited rheological behavior typical of a weak gel, which is expected to be advantageous as regards the capability of the semisolid preparation to create and maintain the space within the wound and to provide protection to the injured tissue. Results of ex vivo experiments demonstrated that Fitostimoline® Plus was more effective than Connettivina® Bio Plus in promoting both skin hydration and energy.

Evaluation of physical and chemical stability of semi-solid preparations towards beyond-use date

Evaluation of physical and chemical stability of semi-solid preparations towards beyond-use date

Dosage Evaluation and Antioxidant Activity Test of Lotion of Ethanol Extract of Belimbing Wuluh Leaf (Averrhoa Bilimbi L.)

<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><em>This study aims to make and evaluate the lotion formula from ethanol extract of star fruit leaves (Averrhoa bilimbi L.) and test the antioxidant activity of the lotion. The research method used is experimental, making lotions and testing antioxidant activity from July to October 2023 at the Semi-Solid Preparation Technology Laboratory, Department of Pharmacy, Poltekkes Kemenkes Medan, and Research Laboratory, University of North Sumatra. In the preparation stage, star fruit leaf simplisia was extracted by maceration using a 95% ethanol solvent. The lotion formula was made with ingredients such as PEG 4000, stearyl alcohol, glycerin, sodium lauryl sulfate, coconut oil, nipagin, and distillate water, with varying concentrations of ethanol extract of star fruit leaves. The formulation was evaluated through organoleptic, homogeneity, pH, spreadability, and viscosity tests. The results showed that the lotion produced had a homogeneous shape and aroma to the concentration of ethanol extract. In addition, the pH of the lotion is also within the safe range for human skin. The spreadability test showed the lotion's ability to spread on the skin, which met the requirements for lotions with spreadability values between 5 and 7 cm. The viscosity of the lotion was also by the set standards. Stability tests were conducted to monitor the physical and chemical changes of the lotion over four weeks. The results showed that lotions with 1% and 3% ethanol extract concentrations were stable during the study period. However, the 5% concentration experienced inhomogeneity changes in the third week. In conclusion, lotion with ethanol extract of star fruit leaves at 1% and 3% concentration can produce a good and stable formula. However, the antioxidant activity of the lotion is still in the weak to moderate category. This research contributes to developing plant-based natural cosmetic products for skin care.</em><em></em></p><p><em> </em></p></td></tr></tbody></table></div><p><strong><em>Keywords: Antioxidant Activity, Belimbing Wuluh, Ethanol Extract, Evaluation, Lotion</em></strong></p>

Effect of stearyl alcohol on imiquimod-induced psoriasis-like skin inflammation in mice.

Psoriasis is a chronic inflammatory skin disease. Topical medicines are the preferred treatment for mild to moderate psoriasis, but the effect of excipients used in semi-solid preparations on psoriasis-like skin inflammation is not fully understood. In the present study, we investigated the effect of stearyl alcohol, a commonly used excipient, on imiquimod (IMQ)-induced psoriasis-like skin inflammation in mice. Psoriasis-like skin inflammation was induced by topical IMQ treatment on the back of mice. Skin lesion severity was evaluated by using psoriasis area and severity index (PASI) scores. The skin sections were stained by haematoxylin-eosin and immunohistochemistry. Stearyl alcohol (20% in vaseline) treatment significantly reduced the IMQ-induced increase of PASI scores and epidermal thickness in mice. IMQ treatment increased the number of Ki67- and proliferating cell nuclear antigen (PCNA)-positive cells in the skin, and the increases were inhibited by stearyl alcohol (20% in vaseline) treatment. Stearyl alcohol treatment (1%, 5%, 10% in vaseline) dose-dependently ameliorated IMQ-induced increase of PASI scores and epidermal thickness in mice. Hexadecanol (20% in vaseline), stearic acid (20% in vaseline) and vaseline treatment had no significant effect on IMQ-induced psoriasis-like skin inflammation in mice. In conclusion, stearyl alcohol has the effect of improving IMQ-induced psoriasis-like skin inflammation in mice.

Equivalence assessment of creams with quali-quantitative differences in light of the EMA and FDA regulatory framework

EMA and FDA are upgrading guidelines on assessing the quality and the equivalence of topically applied drug products for developing copies of originator products and supporting post-marketing variations. For topical products having remarkably similar composition, both EMA and FDA accept the equivalence on the bases of the comparison of rheological properties and in vitro drug release constant (k) and skin permeation flux (J) values, instead of clinical studies. This work aims to evaluate the feasibility to expand this approach to variations of the composition of complex semi-solid preparations. Ibuprofen (IB) creams at two different strengths (i.e., 1 % and 10 %) were used as a model formulation. Two formulative changes were performed: (a) the addition of the humectant to simulate a minor post-marketing variation; (b) the substitution of the emulsifying system to simulate a major one. These variations impacted only in 1 % IB formulations where both the equivalences of rheological data and J-values failed. At the highest concentration, the presence of IB crystals broke down the differences in rheological patterns and lead the IB thermodynamic activity at the maximum figuring out an overlapping of the J-values. Such data suggest the combination of these studies, which are thought mainly for the development of copies, could be also applied to the management of post-marketing variations that involve product composition.

Organogel as Drug Delivery System: Review

Background: Gels are employed as a carrier medium/network for the delivery of topical medicines. Due to number of benefits gels are gaining attention day by day as a drug delivery system. Among gels Organogelare preferably active for incorporating lipophilic and hydrophilic drugs within the same gel network. Aim: To emphasize about the basics of Organogel. Their introduction types, composition, properties, methods of preparation and advantages and applications and most importantly as topical drug delivery system. Definition: The Organogel comes under the semi solid preparations with a definition of having two phases,one of which is organic liquid which gets immobilized by the other, that is an organogelators. Composition: Organogel are, “mixture of lipophilic liquid and solid lipid, in which solid lipid having concentrations (<10%) can entangle bulk liquid oil by formingthreedimensionalnetwork by the physical interaction of self-assembled organogelatorsin the bulk oil”2. Properties: Generally Organogel are thermodynamically stable system, viscoelastic, biphasic system, no irritating, have low degree of hydration in comparison to hydrogels. Exhibit morphological and rheological properties Method of Preparation: Solid fiber Matrix and fluid filled matrix are most common methods adopted for Organogel preparations. Characterization: viscoelasticity, pH, Firmness, In vitro drug, release, stability, phase transition, and structural features,Invitro release, skin permeability were discussed along with different factors that affectOrganogel. Applications: Organogel are used for both oral and topical delivery of number of drugs. They are used for local as well as are capable of systemic percutaneous absorption due to their lipophilic nature and penetration potentiates by the presence of penetration enhancer3. Keywords: Organogels, Organogelator, Solvents, Drug delivery system

Surface enhanced Raman scattering microtips for microenvironment pH determination of semi-solid preparations.

Semi-solid preparations such as ointments, creams, gels, and pastes are common topical dosage forms with complex compositions and microstructures. pH is a critical quality attribute for semi-solid preparations, affecting properties such as particle size distribution, drug dissociation state, and rheological behavior. Currently, traditional pH electrode methods only provide an "average" pH for large-volume matrices, with low spatial resolution and accuracy. Microenvironment pH detection is crucial for accurately assessing semi-solid preparations. Herein, we developed pH-sensitive surface enhanced Raman scattering (SERS) microtips to achieve localized pH detection in semi-solid preparations. SERS microtips were prepared from glass needles with a tip size of around 1 μm and gold nanoparticles (Au NPs) grown in situ on glass surfaces for SERS enhancement. 4-Mercaptopyridine was selected as a pH sensitive Raman reporter and immobilized on the Au NPs, exhibiting characteristic Raman peak shifts within the pH range of 3-10. The SERS microtips were employed to conduct highly sensitive pH measurements in localized areas of 15 commercial ointments, 8 gels, and 1 laboratory-made ointment, providing higher spatial resolution and microenvironment differentiation compared to pH meters. The SERS microtips were used to monitor pH changes over time in ointment applied to localized wounds on live mice. This work introduces a new tool for pH detection in semi-solid preparations, offering a new method to enhance the prescription process and quality assessment of complex preparations like topical semi-solid preparations.

Formulasi Dan Evaluasi Sediaan Salep Luka Bakar Basis Hidrokarbon Ekstrak Etanol Daun Katang-Katang (Ipomoea Pescaprae L.) Asal Desa Seith Tahun 2023

Background: Ointment is a semisolid preparation used as a topical medicine, which has direct access to the skin tissues. Katang-katang is one of the medicinal plants traditionally used to treat various types of wounds. Objective: The aim of this study is to identify the secondary metabolite content of katang-katang leaves (Ipomoea pescaprae L.), create and evaluate a good and stable ointment formulation pharmaceutically, and test the irritation of the ointment for burn wounds using ethanol extract of katang-katang leaves (Ipomoea pescaprae L.). Method: The method used is experimental research, the ointment formulation is made using three formulations with different concentrations of active ingredients, with a ratio of FI 20%, FII 25%, and FIII 30%. Result: The results of the study indicate that katang-katang leaves contain flavonoids, tannins, and steroids. The study determined the formulation of a burn ointment from ethanol extract of katang-katang leaves (Ipomoea pescaprae L.). The organoleptic test results of FI, FII, and FIII showed good and stable color, odor, and shape, as well as homogeneity after one week of storage. The pH values measured were 7 for all formulations even after one week of storage. The spreading power of FI was (5.52 cm), FII was (5.88 cm), and FIII was (7.23 cm), which met the standards for good formulation. The irritation tests for all three formulas were categorized as safe. Conclusion: Based on the identification test results, the katang-katang leaves were found to contain flavonoids, tannins, and steroids. The evaluation test results for the three formulations using a hydrocarbon base showed that a good and stable burn ointment could be made from the ethanol extract of katang-katang leaves (Ipomoea pescaprae L.) without causing skin irritation.

Veterinary compounding: the impact of different gelling agents on the rheological characteristics and release kinetics from meloxicam oral gels in the treatment of cats

Worldwide, the growing number of pets has increased the use of veterinary drugs, as well as individualised therapy such as compounded drugs. If a suitable formulation or drug is not registered for veterinary species, drugs are compounded. This also includes pharmaceutical forms which are easy to administer, such as semi-solid preparations (gels and pastes) for oral use in order to enhance drug administration and adherence. The number of non-steroidal drugs (NSAIDs) approved for use in cats is relatively small. NSAIDs represent the largest group of drugs with adverse drug experience, but meloxicam has advantages in relation to all of them because of its safety for use in cats. In this study, the pharmaceutical-technological characterisation of oral gels for cats made with different gelling agents (carbomer, xanthan gum, carmellose sodium and hypromellose) was undertaken. Subsequently, four different samples were prepared and designed for veterinary use (using meloxicam, gelling agents and taste enhancer), and then the pH value, rheological characterisation and drug release were determined. The results demonstrated that addition of the taste enhancer influenced the rheological properties of the tested oral gels. It was found that the rate of meloxicam release was the highest from the oral gel prepared with carbomer (Carbopol® 974P), where complete meloxicam dissolution was observed after 5 minutes. On the basis of these results, it was concluded that Carbopol 974P has a certain advantage over the other gelling agents used in this study. Further work is required to assess the palatability of the oral gel for cats in clinical settings.

Unraveling solid–liquid phase transition and microstructural coarsening of semi-solid Al0.8Co0.5Cr1.5CuFeNi HEA with dual globules for thixoforming application

Multi-phase HEAs typically have potential of semi-solid processing due to wide semi-solid range. For thixoforming route of semi-solid processing (SSP), the semi-solid billet preparation by semi-solid isothermal heat treatment (SSIT) is one of the most important process steps. This work focuses on the microstructure evolution during SSIT of a semi-solid Al0.8Co0.5Cr1.5CuFeNi HEA with dual globules for thixoforming application. This semi-solid HEA contains BCC(B2), FCC globules and Cu-rich liquid film. Solidified liquid follows K-S, epitaxial orientation relationship withneighboringBCC(B2) and FCC globules. Solutionizing point for B2 phase is higher than roughly 1175 °C, and complete disordering of BCC(B2) globule occurs at 1225 °C. While, FCC globule persists stable disordered solid solution. Solid-liquid phase transition of globules provides liquid film proliferation. CALPHAD simulation on the “hypothetical” alloy system corresponding to component constitution of individual globule successfully forecasted solid–liquid phase transition. Coupling coarsening inhibition effects, including sluggish diffusion in globules, Cu-rich liquid film as inhibitory barrier, heterogeneous globule as obstacle, account for extremely sluggish microstructural coarsening (coarsening rate is only 2.44–11.25 μm3/s). Finally, through forming ofexemplar component, intriguing potential for SSP application in component manufacturing was verified.

Formulation and investigation of hydrogels containing an increased level of diclofenac sodium using risk assessment tools

Transdermal delivery of active ingredients is a challenge for pharmaceutical technology due to their inadequate penetration properties and the barrier function of the skin. The necessity of painless, effective, topical therapy for the aging population is growing, and a variety of diclofenac sodium-containing semi-solid preparations are available to alleviate the symptoms of these ailments.Our purpose was to formulate a novel composition with higher drug content to enhance drug release and permeation, thereby providing more effective therapy. Another goal was to maintain the concentration of the organic solvent mixture below 30%, to protect the skin barrier.Firstly, literature and market research were conducted, based on which the appropriate excipients for the target formulation were selected. Solubility tests were conducted with binary and ternary mixtures. As a result, the optimal ternary mixture was chosen. Hydrogels containing 1, 5, and 7% of diclofenac sodium were prepared and the stability of the formulations were studied by microscopic measurements and cytotoxicity test were carried out of the components also. The release and permeation of diclofenac sodium were investigated in different concentrations.It can be concluded that we have succeeded in preparing a topically applicable stable diclofenac sodium hydrogel with higher concentration, drug release, and improved skin permeation than the formulations available on the market.

Efikasi salep dari ekstrak daun Turi (Sesbania Grandiflora) sebagai penyembuhan luka bakar terhadap Kelinci (Oryctolagus Cuniculus)

Background: Burns are frequent injuries and can cause serious health problems. Turi leaf extract (Sesbania Grandiflora) has been used as a traditional medicine to treat burns. This study was conducted to determine the effectiveness of ointment from turi leaf extract in healing burns in rabbits.Objectives: This study aimed to evaluate the effectiveness of turi leaf extract cream in rabbits' recovery burnsMethods: The study uses experimental research methods with a single factor design. (single factor design). As for the location of this research, it was carried out in the Pharmaceutical Technology Laboratory of semi-solid preparation, Pharmacy Studies Program of the Faculty of Health Sciences, Muhammadiyah University of Palopo. The study was conducted in the period January-February 2023. The sample used is fresh turia leaves (Sesbania Grandiflora) that are green and cleaned or disorted, then washed with running water until clean. Samples of turi leaves are dried with winding for 3-5 days. The sample is then sprayed with a blender. The data to be analyzed is the percentage of burn healing obtained by measuring the average diameter of the burn. Measurement is done once a day.Results: The results of the study showed that rabbits who were given turia leaf extract ointment had a faster burn healing process compared to the rabbit who was given regular ointments. The area of burns in rabbits given turia leaf extract ointment is also smaller than the rabbit given regular ointments. The ointment from the turia leaf extract is effective in healing burns in rabbits. The results of this study showed that on the 14th day, burns in mice given the FIII dose group (concentration of 7,5%) had the greatest effect on wound healing, followed by the FII dose group (5%) which had a 99,70% healing percentage. The FI dose group had a healing percentage of 85,69%, a positive control of 90,15%, and a negative control of 63,29%.Conclusion: it can be concluded that the ethanol extract of senggani leaves has the potential to heal burns in rats. The FIII dose group that had the highest concentration showed the greatest effect on wound healing. However, the FII dose group with lower concentrations also showed a significant effect in healing burns in rats.

Preparation and Evaluation of Herbal Cream containing Anethum sowa and Aloe barbadensis

Dill (Anethum sowa) is known as aromatic, medicinal and culinary herb since ancient times. The extraction of essential oil and the chemical composition of oils from dill seeds are described along with antimicrobial, insecticidal, antifungal and other uses. Aloe vera is known for its skin care and medicinal properties since time immemorial the plant is extensively recognized as a remedy for rashes, dry skin, allergic irritation, skin aging, wound healing, psoriasis, dermatitis and inflammation. Creams are semisolid preparation that contains one or more medicaments usually in a base with refreshing fragrances and are intended to spread on skin easily. The effectiveness of these products depends in part on your skin type and the active ingredient. Staphylococcus aureus (S. aureus), a bacterium that causes skin infection as a result of skin colonization; it is becoming increasingly resistant to many commonly used for antibiotics. The anti-bacterial activities were proportional to the concentration of extract alone and in the cream. All cream formulations showed satisfactory physical properties with smooth texture, emollient, non- greasy and easy to remove with water. It is concluded that Anethum Sowa and Aloe barbadensis has potential to be developed as a cream for skin infections caused by S.aureus. The zone of inhibition is seen on the cup plate method and the reading is compared with the standard. The evaluation is done on the formulation. There studies suggest that composition of extract and base of cream of F3 and F4 are more stable and safer, it may produce synergistic action.

The Utilization of Secang Wood (Caesalpinia sappan L.) Extract Nanoemulsion as Natural Pigment in Lip Cream Preparations

Lip cream is a semi-solid lipstick preparation. One of the important ingredients in lipcream preparations is a variety of colorants. Secang wood contains the active compound brazilin which gives it a yellow to purple color which can be used as a natural dye. The conventional lip cream preparations from extracts have low homogeneity and unstable, so it is necessary to increase the stability of the preparation by making a nanoemulsion of secang wood extract which is incorporated into the lip cream preparation. The preparation of 10% secang wood extract nanoemulsion preparation using Tween 80, Virgin Coconut Oil (VCO), and PEG 400 with particle size reduction using a bath sonicator. The concentration of the nanoemulsion preparation was 1% (F3) and 3% (F4) incorporated into the lip cream base and conventional lip cream preparations with the same concentration (F1 and F2). The test results for the size of the 10% secang wood extract nanoemulsion globules were 127.54 ± 0.45 nm (PI: 0.3 ± 0.013). The test results for the F3 and F4 nanoemulsion lipcream preparations had a pink to purple color, pH 6-6.4, spreadability of 5.9-6.8 cm, adhesion of 5.5-6 seconds, the preparation was homogeneous and non-irritating, compared with conventional lip cream preparations F1 and F2 are not homogeneous, the color of the lip cream is paler, and the irritating properties are very mild in both formulas. The conclusion of this research is that secang wood extract can be formulated into a nanoemulsion preparation and incorporated into a lip cream preparation.
 Keywords: Brazilin, Color, Dye, Sonicator Bath