Semi-natural Landscapes Research Articles

High soil moisture promotes the emergence of ground beetles and spiders from soils in wheat fields

Promoting arthropods in agricultural landscapes can contribute substantially to stop their decline and enhance pest control. Higher soil moisture and the presence of field margins can increase the abundance of arthropods in agricultural landscapes and influence their distribution within crop fields. However, little is known about the influence of soil moisture and distance from field margins on the overwintering of arthropods in arable fields. We investigated the influence of soil moisture and distance from a field margin on the numbers of arthropods, ground beetles and spiders emerging from soil in winter wheat fields. We established transects in winter wheat fields away from two different types of field margins: (i) around small standing water bodies (kettle holes) to capture a wide range of soil moisture values and (ii) other semi-natural landscape elements. At three distances (1 m, 20 m, 50 m), we sampled arthropods with emergence traps and measured soil moisture between March and June. We found that soil moisture had a positive effect on the emergence numbers of arthropods in general and ground beetles and spiders in particular. Distance from field margins generally had negative effects on the emergence numbers of ground beetles, but positive effects on the emergence numbers of spiders. Emergence numbers and soil moisture content did not differ significantly between the two types of field margins. The high emergence numbers inside the fields indicate that arable fields are important overwintering habitats for beneficial arthropods. Proper management of arable soils to promote soil water holding capacity and soil moisture content may have the added benefit of promoting the production of beneficial natural enemies from local soils.

Bird Diversity on an Under-Constructed Educational Campus: A Case Study of Nalanda University, Rajgir, India

Bird species have been critically impacted by increasing anthropogenic activities such as infrastructural construction. Academic institutions with dedicated semi-natural landscapes may provide shelter to high bird diversity. However, assessing the bird diversity of an under-construction campus becomes critical for future comparison. This study was conducted at Nalanda University Campus, India, for five months, as the campus was at the fag-end of the construction phase with diverse land use changes. 91 bird species were found on campus primarily dominated by insectivores and omnivores. Occurrence, and migratory status of the species revealed that 47% were resident, primarily occupying aquatic habitats. Despite presence of Least Concern IUCN status bird species in the campus, substantial number of them had a declining abundance index at the national level. Sites with high land conversion showed a low number of bird species. The overall dominance and evenness highlighted a good bird diversity, contrary to altered sites where both these attributes were high. Data for the documented bird species on citizen science platforms highlighted limited information on species interactions. This study attempted to bring attention to the unchecked impact of construction activities on bird diversity and regular monitoring in the widely discussed emerging semi-natural shelters, i.e., academic campuses.

Wildflower strips of 2.5-year-old promote earthworms and enchytraeids (Annelida, Oligochaeta) in arable fields

In the last 50 years, humans have increased crop yields due to intensive agricultural practices and by homogenizing cultivated lands (e.g., larger and more uniform fields). However, this land management practice has led to serious environmental issues, and now, the importance of heterogeneity and semi-natural landscape elements in production systems is acknowledged. Perennial habitats, such as flower strips, could play a key role in agroecosystem sustainability, but little is known about their effects on earthworm and enchytraeid (Annelida: Oligochaeta) communities. The aim of this study was to assess earthworms and enchytraeids in 2.5-year-old wildflower strips that were sown in the middle of arable fields in northern France. Samples (soil, earthworms and enchytraeids) were collected at ten locations, in flower strips and in adjacent cropped fields. The same number of earthworm species was found in both habitats, but more enchytraeid species were detected in the flower strips than in the adjacent cropped fields. Moreover, the total abundance of earthworms and enchytraeids significantly increased in the flower strips compared with the adjacent cropped fields, by 69 % and 61 %, respectively. Flower strips had a significant positive effect on anecic and endogeic earthworms but not on the abundance of epigeic earthworms, which was highly variable among the samples, although on average, it was seven times greater in the flower strips than in the cropped fields. Although the flower strips were sown only 2.5 years earlier, significant changes were observed in the soil Oligochaeta communities. These findings advocate for sowing flower strips within cultivated land as a source of soil biodiversity in the current changing environment. Considering the positive role of flower strips on biodiversity and particularly on the studied tiny soil engineers, these perennial landscape elements should be more widely considered to support the agroecological transition.

Seminatural areas act as reservoirs of genetic diversity for crop pollinators and natural enemies across Europe

AbstractDespite increasing recognition of the importance of the multiple dimensions of biodiversity, including functional or genetic diversity as well as species diversity, most conservation studies on ecosystem service‐providing insects focus on simple diversity measures such as species richness and abundance. In contrast, relatively little is known about the genetic diversity and resilience of pollinators or natural enemies of crop pests to population fragmentation and local extinction. The genetic diversity and demographic dynamics of remnant populations of beneficial insects in agricultural areas can be a useful indicator proving additional insights into their conservation status, but this is rarely evaluated. Although gene flow between agricultural and seminatural areas is key to maintaining genetic diversity, its extent and directionality remain largely unexplored. Here, we apply a pan‐European sampling protocol to quantify genetic diversity and structure and assess gene flow between agricultural and nearby seminatural landscapes in populations of two key ecosystem service‐providing insect species, the lady beetle Coccinella septempunctata, an important predator of aphids and other crop pests, and the bee pollinator Andrena flavipes. We show that A. flavipes populations are genetically structured at the European level, whereas populations of C. septempunctata experience widespread gene flow across the continent and lack any defined genetic structure. In both species, we found that there is high genetic connectivity between populations established in croplands and nearby seminatural areas and, as a consequence, they harbor similar levels of genetic diversity. Interestingly, demographic models for some regions support asymmetric gene flow from seminatural areas to nearby agricultural landscapes. Collectively, our study demonstrates how seminatural areas can serve as genetic reservoirs of both pollinators and natural enemies for nearby agricultural landscapes, acting as sources for recurrent recolonization and, potentially, contributing to enhancing ecosystem service and crop production resilience in the longer term.

Synergic effects of vegetation structure and food supply underlie higher abundance of a farmland specialist bird in organic than in conventional arable fields

Organic farming is considered beneficial for farmland biodiversity. However, it is often unclear what specific factors are underlying the biodiversity benefits of this farming practice. The positive effects may be driven by factors acting at the field level represented by, for instance, the absence of pesticides, or by the factors acting at the level of entire farms represented by a higher diversity of crops and intercrops types, and higher share of semi-natural landscape elements. Distinguishing between these two groups of factors is difficult because they are confounded in heterogeneous landscapes consisting of small arable fields. However, their separation is crucial to understand the origins of organic farming effectiveness. The landscape in those Central and Eastern European countries that have undergone collectivization, such as Slovakia, Czechia or Lithuania, consists of large fields cultivated under either conventional or organic regime. These circumstances are suitable to assess the sole influence of in-field factors providing potential biodiversity benefits of organic farming. For this purpose, we selected a study area in the lowlands of the south-western Slovakia, where conventional and organic fields were situated in close proximity. Such a spatial arrangement was suitable to examine the possible influence of farming practice (i.e. organic vs. conventional) itself, minimising the influence of landscape elements. We focused on a single cereal crop, winter wheat, cultivated on both organic and conventional fields. Here, we counted the breeding abundance of the Eurasian Skylark (Alauda arvensis), a farmland specialist bird closely associated with arable fields providing both breeding and feeding habitat for this species, which may be considered as a farmland biodiversity indicator. Skylark counts were higher in organic than in conventional wheat fields throughout the season. This difference was statistically related to a higher abundance of invertebrates in samples collected in organic fields than in conventional fields. However, Skylark counts were also related to sparser crop cover in organic fields, especially at the end of the season. Sparser crop cover may improve nesting conditions and facilitate foraging. Our results indicate that organic farming may have a positive impact even at the field-level. The benefits for the Skylark, and possibly for the other farmland organisms, can be provided by the joint effects of the absence of pesticides and a suitable vegetation structure. Organic farming can thus be a suitable agri-environmental measure within the Common Agricultural Policy even in intensively used landscapes dominated by large fields.

Spatial planning constraints will mitigate the fragmentation trajectory of natural and semi-natural landscapes: a case of Lushan City, China

ContextThe biodiversity faces an underlying threat from landscape fragmentation resulting from rapid urbanization. Examining the future trajectory of landscape fragmentation is imperative to understanding the impact of current spatial planning constraints on natural and semi-natural habitat preservation, ecosystem services, and sustainability.MethodsWe employed a Patch-generating Land Use Simulation (PLUS) model to simulate and predict the land use and landscape pattern changes in Lushan City under two distinct scenarios: “Planning Constraints (PC)” and “Natural Development (ND)”. We then identified an appropriate landscape fragmentation index (LFI) that effectively captured the fragmentation essence. To determine the optimal scale, we adopted an experimental approach using both the moving window (MW) method and the semi-variance function. By constructing a LFI spatiotemporal sequence and conducting trend analysis, we identified the potential fragmentation areas (PFA) with significant fragmentation tendencies.ResultsThe spatial planning constraints will (1) prevent the encroachment of construction land into 2.14 km2 of cropland, 0.21 km2 of forest, and 0.13 km2 of grassland; (2) shift the highly fragmented area from the northeastern portion of Lushan to the planned area defined by the development boundary; (3) mitigate and decelerate the trend of landscape fragmentation in natural and semi-natural landscapes, decrease PFA by 7.74 km2 and preserve 15.61 km2 of natural landscapes. (4) still leave 29.42% of forest and 22.82% of grassland at risk of fragmentation.ConclusionsSpatial planning constraints will effectively control the potential fragmentation in natural and semi-natural landscapes by changing the spatial distribution of LFI and PFA. This control mechanism will greatly exclude the anthropogenic impact and ensure the conservation of habitats. The habitats remaining within PFA should be focused in future eco-management optimization.

As green infrastructure, linear semi-natural habitats boost regulating ecosystem services supply in agriculturally-dominated landscapes

Semi-natural linear landscape elements, such as hedgerows, are vital structures within agricultural landscapes that have an impact on ecosystem processes and support biodiversity. However, they are typically omitted from green infrastructure planning, which could lead to significant undervaluing of landscapes and their multifunctionality in terms of ecosystem service supply. Using the InVEST (Integrated Valuation of Ecosystem Services and Tradeoffs) model suite, we tested the effects of additionally including semi-natural linear landscape elements on the model outcomes for crop pollination, nutrient regulation, erosion regulation and water flow regulation ecosystem services supply. The results showed that linear semi-natural landscape elements contribute positively to the landscape’s multifunctionality. Small changes have been identified for water flow regulation, whereas, considering both spatial extent and magnitude of the changes, the greatest changes have been found with respect to the supply of pollination and nutrient regulation. Direct proximity of the linear elements had the greatest effect on ecosystem service supply, in particular with regard to pollination. Based on our results, a more pronounced consideration of semi-natural linear landscape elements as an important element of green infrastructure is advisable.

Owl-mediated diploendozoochorous seed dispersal increases dispersal distance and supports seedling establishment

Seed dispersal is an essential process contributing to the maintenance of plant populations. Zoochory is a widespread way of plant dispersal in every terrestrial ecosystem that can ensure the long-distance dispersal of seeds. Secondary seed dispersal (SSD) by far-ranging raptors is a special type of zoochory, which might have a role in colonizing new habitats. We used the barn owl (Tyto alba) as model species to test the effectivity and seasonality of SSD in open semi-natural landscapes. We collected 582 pellets from six sites in East-Hungary throughout one year. We identified prey items in the pellets and determined the viable seed content of the pellets by germination experiments. We found that herbivorous Microtus arvalis L. was the most abundant prey item through which most of the seeds spread. Owls dispersed the seeds of generalist and disturbance-tolerant plants, indicating the habitat type where small mammals occur abundantly. In another experiment we tested the effect of the pellet material on the seedling survival and found that prey remains enhanced establishment of seedlings. Our study suggests that SSD by barn owl is occasional but important event in long-distance seed dispersal. Since the studied owl species uses several habitat types and has larger mobility than the rodents, the revealed dispersal mechanism can considerably increase seed dispersal distance and seed exchange between habitat types.

The impact of urban green space landscape on PM2.5 in the central urban area of Nanchang city, China

With China's rapid urbanization and industrialization, PM2.5 as a significant air pollutant, has drawn considerable public attention and strengthen the public's awareness of air quality. As a semi-natural landscape, many studies indicated that urban green space could influence the concentration and distribution of PM2.5 to a certain extent. However, most current researches were conducted at population or community scale, and how the urban green space affects PM2.5 at landscape scale was seldom studied. Taking the central urban area of Nanchang city, China, as a case, this study firstly encrypted the monitoring points by the land use regression model (LUR), and simulated the spatial distribution of PM2.5 concentration with high-precision based on the ordinary kriging interpolation method. Then, based on the urban green space data interpreted by remote sensing, the influence of urban green space landscape characteristics on PM2.5 concentration was quantitatively analyzed. The results showed that: (1) The PM2.5 concentration in the study area decreases continuously from the city center to the periphery; (2) Landscape pattern of green space has a significant effect on PM2.5 concentration. Patch green space has a stronger effects on PM2.5 concentrations than corridor green space did; (3) The attributes of area and NDVI of green space have a significant negative correlation with PM2.5 concentration. (4) The effective distance that green space can affect PM2.5 in the study area was less than 100 m. This study is expected to provide some references for lightning PM2.5 pollution by means of optimization of urban green space.

Decline in Semi-Natural Grasslands and Changes in Value Perceptions in Japan’s Natural Parks

The decline in semi-natural grasslands has resulted from reduced traditional use, leading to a marked decrease in their overall area. Despite being located within Japan’s core protected area system, the effectiveness of natural parks in conserving grasslands remains unclear. This study aimed to determine whether natural parks successfully prevent the decline in grasslands. By analyzing historical changes in value perceptions toward grasslands in natural parks, natural parks’ contribution to grassland conservation and the associated challenges were explored. The study focused on the Sengokuhara grassland in Fuji-Hakone-Izu National Park and incorporated previous studies on other grasslands. It revealed that grasslands have been valued for their scenic views and recreational potential since the establishment of the national park. As grassland scarcity increased nationwide, attention shifted toward rare plants found exclusively on grasslands, along with grasslands’ value as semi-natural landscapes. Consequently, natural-park-based grasslands have primarily been conserved based on these two values. However, conservation efforts within natural parks have mainly focused on regulating human activities, with limited measures to sustain nature, such as grasslands, through livelihoods. To enhance grassland conservation, it is essential to recognize their cultural value and develop mechanisms that ensure economic benefits are reinvested in conservation efforts.

Host Preferences of Spotted Lanternfly and Risk Assessment of Potential Tree Hosts in Managed and Semi-Natural Landscapes

Host Preferences of Spotted Lanternfly and Risk Assessment of Potential Tree Hosts in Managed and Semi-Natural Landscapes

Development of soil health benchmarks for managed and semi-natural landscapes

Efforts to improve soil health require that target values of key soil properties are established. No agreed targets exist but providing population data as benchmarks is a useful step to standardise soil health comparison between landscapes. We exploited nationally representative topsoil (0–15 cm) measurements to derive soil health benchmarks for managed and semi-natural environments across Great Britain. In total, 4587 soil organic matter (SOM), 3860 pH, 2908 bulk density (BD), and 465 earthworm abundance (EA) datapoints were used. As soil properties are sensitive to site-specific characteristics, data were stratified by habitat, soil type, and mean annual precipitation, with benchmarks defined as the middle 80 % of values in each distribution – yielding 135 benchmarks. BD and pH decreased with land management intensity (agriculture > semi-natural grasslands > woodlands > heathlands > wetlands), and vice versa for SOM and EA. Normalising benchmark ranges by medians revealed soil health indicator benchmark widths increased in the order: pH < BD < SOM < EA, while width increased with decreasing land management intensity. Arable and horticulture and improved grassland exhibited narrow benchmarks for SOM, pH and BD, yet the widest EA benchmark, suggesting additional drivers impact EA patterns. Upland wetlands had the widest BD benchmarks, important when determining carbon stocks. East Anglia currently possesses the largest proportions of atypical soils, including below typical SOM (19.2 %), above typical BD (17.4 %) and pH (39.1 %), and the smallest proportions of above typical SOM (2.4 %), and below typical BD (5.8 %) and pH (2.3 %). This is found even after land use, soil type and rainfall have been considered, underscoring how urgently soil health should be addressed here. Our benchmarking framework allows landowners to compare where their measured soil health indicators fall within expected ranges and is applicable to other biomes, national and multinational contexts.

What drives the risk of being bitten by a viper? A fine spatial scale study in western France

Previous studies on viper bites in France have focused on clinical consequences of envenomation, efficacy of antivenom and epidemiology of bites. Herein, we wanted to clarify temporal and spatial patterns in bite incidence using a fine spatial scale (municipality level). We focused on viper bites recorded over the last 10 years in 4 regions of western France. We addressed the determinants of bite occurrence and number of bites considering the following variables: predicted probability of viper presence, species (V. aspis or V. berus), climatic data, tourism function rate, soil transformation and landscape use. 703 bite cases were retained with significant disparities between areas. Bites occurred either during a garden-related activity (339 cases, 51.2%) or during an activity in the countryside (300 cases, 45.3%). The probability of presence of a viper at the municipality level positively influenced the risk of being bitten (multiplied by 3 for a variation in probability of 0.25 from 0.5) but varied between species (lower in V. berus than V. aspis). Artificial land development had a positive effect on bite risks. Finally, a tourism function rate above 50 beds/100 inhabitants was strongly associated with an increase in the risk of occurrence and frequency of bites. Overall, viper bites recorded in our study were concentrated on the south coastline of Pays de la Loire region. The coastal towns are significant areas of tourist attraction and are located close to preserved semi-natural landscapes that provide favorable habitats for vipers. This convergence may favor human/wildlife encounters.

Integrating perceptions of ecosystem services in adaptive management of country parks: A case study in peri-urban Shanghai, China

As a type of peri-urban green space with natural environments and countryside features, country parks provide numerous ecosystem services (ES) for human beings. In China, the multifunctional characteristics and diverse social groups related to the country park construction in the context of urbanization indicate that these ES need to be managed in the social dimension through adaptive decision-making. However, little work has focused on the perceptions of ES for country park management. In this study, we developed a three-step research framework aiming to integrate ES perceptions in adaptive country park management. We carried out face-to-face photo-elicitation questionnaire surveys (n = 229) in a country park pilot in Shanghai to analyze the perceptions of ES by park users. Multiple analytical methods, including statistical analyses, importance-performance analysis, and ES indexes, were applied to reveal the linkages among perceived ES, users’ characteristics, and specific landscape features. Our results showed that water and air quality maintenance, aesthetic, habitat, and physical and mental health, were perceived as the most important, highest performance, and highest priority ES. Users’ education level and their self-reported knowledge about the country park were the two most important variables that explained perceived ES. Perceptions of user groups differed along the distance of the place of residence from the study area, with locals assigning high priorities to provisioning ES and cognitive cultural ES and more distant visitors assigning high priority to education. Semi-natural landscape features, such as lakes and rivers, marsh, swamp forest, and lotus ponds, were perceived as providing the most ES. We further communicated and discussed the results with managers and experts, and provided insights for the next stage of management. We argue that adaptive management should be woven toward environmental justice by understanding the diverse perspectives and views of users on ES.

Response of ants, beetles and spiders to disturbance varies among taxa in a South African savannah biome

Savannahs are structurally complex ecosystems consisting of a diverse community of plants and animals such as arthropods. Arthropods are essential in many ecosystem processes that help maintain life on Earth. The anthropogenic conversion of natural landscapes into croplands, residential and industrial areas has a negative impact on surface-active arthropods that have limited dispersal abilities and narrow habitat preferences. This study investigated the effect of disturbance on assemblages of ants, beetles and spiders in the savannah vegetation in Mpumalanga province, South Africa. We compared species richness, abundance and composition of these three taxa between the pristine savannah and the savannah that is exposed to a variety of anthropogenic activities (disturbed savannah). Arthropods were collected using pitfall traps in 15 sites in pristine savannah and 15 sites in disturbed savannah. We found that disturbance affects species richness and abundance of these taxa differently. Disturbance did not affect species richness of spiders and abundance of beetles, while greater species richness of ants and beetles, as well as abundance of ants and spiders was in disturbed than in pristine savannah. Furthermore, the species compositions of all taxa were different between disturbed and pristine savannah. The disturbed savannah had twice more unique indicator species than the pristine savannah. Differences in assemblages of arthropods between pristine and disturbed habitats suggest that it may be important to consider habitats in and outside protected areas in the conservation of arthropods, particularly in areas with greater percentage of natural and semi-natural landscapes occurring outside protected areas.

Emergency‐line calls as an indicator to assess human–wildlife interaction in urban areas

AbstractHuman–wildlife interactions (HWIs) are increasingly common human disturbances as development continues to remove wildlife habitats. Documenting HWI is critical for environmental protection agencies to develop strategies and management decisions that meet the needs of both people and wildlife. However, evaluation of the frequency and types of HWI at broad spatial scales (e.g., national or regional level) can be costly and difficult to implement by managers. In this study, we apply a novel method for the evaluation of patterns of HWI in urban areas by using publicly available data from emergency calls (ECs) placed by inhabitants of Romania's urban areas. We used information from 4601 ECs placed at the Romanian National Emergency Call System 112, which consisted of (1) wildlife species, (2) spatial location, (3) date and time, and (4) a short description of the emergency. Of the 318 analyzed cities, 300 cities documented ECs on HWI between 2015 and 2020, with roe deer and brown bear being the most frequently mentioned species. We recorded an increasing trend in HWI‐related ECs in 73% of the urban areas over the five‐year period. We mapped the large‐scale distribution of HWI by species and type of interactions in order to capture variations at the national level. Further, we analyzed the social and the biophysical factors potentially influencing the occurrence and frequency of HWI. The results showed that social factors have the same effect on all species, while the effect of the biophysical factors varied between species. Particularly, the presence of large natural habitats, represented by forests, influenced the number of calls only for brown bears. Seminatural landscapes with agricultural land have a different influence in terms of effect and significance for the considered species. Our results suggest that publicly available data from ECs can be used for the rapid assessment of HWI and for evaluating trends and predictors of HWI at broad spatial scales.

Place names as indicators of traditional forest management in seminatural landscapes. The case of Cameros (La Rioja, Spain)

Former grazing landscapes, shaped through almost a millennium in the mountains of La Rioja (Spain), have evolved into a mosaic of forests and shrublands once the outstanding transhumance activities collapsed in the mid-20th century due to population decrease. Likewise, agricultural lands were abandoned in a process of ‘rural exodus’ from the countryside to towns within the framework of industrialization and urban sprawl in Spain since the 1960s. However, some place names still indicate these earlier activities, such as the use of fire as a technique for pasture renewal, communal forest thickets [dehesas] temporarily excluded from livestock raising, sustainable management of forests resources (wood, fuel, charcoal), agricultural crops etc. even if nowadays the earlier land uses and covers have changed into recently spread forests. We present a typical selection of examples that show the importance of toponymy in conceiving and explaining the cultural heritage of past seminatural landscapes, particularly of those whose main aspect today is secondary wilderness.

FEATURES OF SEED PROPAGATION OF AILANTHUS ALTISSIMA (MILL.) SWINGLE IN THE MYKOLAIV REGION

This article presents data on seed propagation of Ailanthus altissima (Mill.) Swingle in Mykolaiv region. The materials of the work are the fruits of 20 individuals of A. altissima, collected in 2018, 2020, and 2022 in urbanized ecotopes (parks of of Mykolaiv city) and ecotopes in semi-natural landscapes (surroundings of the village of Veselinove, Mykolaiv region), as well as field observations of plants. It has been established that A. altissima forms fruits with the dimension of length from 2,5 to 4,2 cm (the mean is 3,3±0,01 cm) and with the dimension of width from 0,8 to 1,5 (the mean is 1,2±0,01 cm). The seeds are 0.3-0.5 cm long (average 0.42±0.01 cm), 0.1-0.3 cm wide (average 0.23±0.01 cm). The mass of 1000 seeds is 21 g. The defined carpological feature of A. altissima meets norms. In laboratory conditions, the quality indicators of A. altissima seeds were determined: good quality and viability were 84% and 92%, respectively, germination indicators varied from 80 to 86%, growth energy - from 68 to 80%. It has been established that the seeds of A. altissima are able to preserve their sowing qualities during a 4-year storage period. The reproductive ability of A. altissima in the conditions of the Mykolaiv region is high (6 points). The obtained characteristics of the generative reproduction of A. altissima illustrate the high reproductive potential of the species.

A short exposure to a semi-natural habitat alleviates the honey bee hive microbial imbalance caused by agricultural stress

Honeybee health and the species’ gut microbiota are interconnected. Also noteworthy are the multiple niches present within hives, each with distinct microbiotas and all coexisting, which we termed “apibiome”. External stressors (e.g. anthropization) can compromise microbial balance and bee resilience. We hypothesised that (1) the bacterial communities of hives located in areas with different degrees of anthropization differ in composition, and (2) due to interactions between the multiple microbiomes within the apibiome, changes in the community of a niche would impact the bacteria present in other hive sections. We characterised the bacterial consortia of different niches (bee gut, bee bread, hive entrance and internal hive air) of 43 hives from 3 different environments (agricultural, semi-natural and natural) through 16S rRNA amplicon sequencing. Agricultural samples presented lower community evenness, depletion of beneficial bacteria, and increased recruitment of stress related pathways (predicted via PICRUSt2). The taxonomic and functional composition of gut and hive entrance followed an environmental gradient. Arsenophonus emerged as a possible indicator of anthropization, gradually decreasing in abundance from agriculture to the natural environment in multiple niches. Importantly, after 16 days of exposure to a semi-natural landscape hives showed intermediate profiles, suggesting alleviation of microbial dysbiosis through reduction of anthropization.

Infected grasses as inoculum for Fusarium infestation and mycotoxin accumulation in wheat with and without irrigation

Grasses growing next to agricultural fields influence the Fusarium abundance, the species composition, and the mycotoxin accumulation of wheat plants, especially the field parts directly adjacent to grasses, are highly affected. Grasses are a more attractive and suitable habitat for Fusarium fungi compared to other arable weeds and occur at mostly every semi-natural landscape element (e.g., kettle holes, hedgerows, field-to-field-borders). In our study, we analyzed the ability of a highly Fusarium infected grass stripe (F. graminearum, F. culmorum, F. sporotrichioides) to infect an adjacent wheat field with these species. Results show that the primary inoculated Fusarium species were as well the dominant species isolated from the wheat field. Regarding transects originating from the grass stripe going into the field, the results demonstrate that wheat ears next to the infected grass stripe have a higher Fusarium abundance and furthermore show higher mycotoxin accumulation in the wheat kernels. This effect was highly promoted by irrigation. Therefore, grass stripes next to arable fields must be considered as reservoirs for fungal infections and as a source for a contamination with mycotoxins.