Semi-automatic Blood Research Articles

Impact of various reagents during blood collection from poultry to analyze morpho-biochemical indicators

The work provides experimental data on the impact of anticoagulants (K3 - EDTA, 3.8% sodium citrate and lithium heparin) and coagulation activator on morpho-biochemical indicators in the blood samples of broiler chickens of cross-selection of Agricultural Center “Smena” at the age of 35 days. The studies were carried out with the use of semi-automatic flow analyzer Sinnowa BS-3000P (SINNOWA Medical Science & Technology Co., Ltd, China) and veterinary automatic blood analyzer DF-50 by Dymind Biotech (PRC) with the use of original reagents. The results showed that the most optimal reagent for determination of morphological indicators of the poultry blood was EDTA. The average white blood count (WBC) is lower in blood samples with citrate and heparin in contrast to EDTA by 30.5% and 24.1%, respectively. The average red blood count (RBC) is lower in tubes with sodium citrate (by 37.9 %) and lithium heparin (by 13.8 %) in contrast to K3-EDTA. When the sodium citrate anticoagulant is used, the blood biochemistry results for all positions of the experiment, excluding calcium, are lower than in heparin, and its closest values are lower than in coagulation activator, excluding trypsin activity. That’s why, the most optimal reagents for biochemistry, including for blood enzyme tests, should be considered the tubes with heparin or coagulation activator.

Development of safe semi-automatic and economic blood cross matching using image processing

Abstract Naming the Blood group is an imperative test for blood transfer and innumerable medical intervention. Generally, the medical practitioner confirms blood typing and its cross-matching suitability before transferring the blood from a person to another. Crossmatch is a fundamental test and is required for wherever blood transfer is needed. While handling an accident case or some emergency, the technicians are performing this test in an open environment which may affect the process and sometimes harmful for technicians. In many hospitals and laboratories, these kinds of tests are carried out in manual using micro plate and test tube. It is time-consuming, error-prone and sometimes it ends up with disease infection for laboratory technicians. Moreover, there is no possibility to revisit the same test results because once after marking it they clean the entire test outcomes. To mitigate the above issues, we propose to design and develop semi-automatic and economical blood cross-matching identification device. This device provides the cross-matching results based on the bonding and its image morphology of the captured image. Further, it provides a captured image for further reference.

Ambulatory blood pressure monitoring in children suffering from orthostatic hypertension

BackgroundIt is particularly important to utilize appropriate blood pressure measurement methods to evaluate the changes of orthostatic hypertension (OHT) for children, and this study was designed to analyze the blood pressure type in OHT children with 24 h semiautomatic ambulatory blood pressure monitoring.MethodsChildren who were diagnosed by head-up tilt table test as OHT patients (OHT group) and treated or hospitalized in the syncope specialist outpatient unit of the Second Xiangya Hospital of Central South University mainly for syncope or pre-syncope with unknown causes during the October, 2009 to September, 2013 were recruited in the study. Healthy children that came to the hospital for physical examination at the same time period according to age and sex were matched as control group. Semiautomatic ambulatory blood pressure monitoring of every child was recorded. The differences of daytime systolic (diastolic) pressure and night systolic (diastolic) pressure were calculated, and the average systolic pressure and diastolic pressure of the entire day, daytime and night were also calculated, respectively.ResultsThere were 23 boys and 17 girls in OHT group, aging (11.5 ± 1.9) years. There were 22 boys and 18 girls in the control group, aged (10.6 ± 2.4) years. The difference of daytime systolic pressure and night systolic pressure of the control group was higher than that of OHT group, while the average systolic pressure of the whole day, the average diastolic pressure of the whole day, the daytime average systolic pressure, the daytime average diastolic pressure, the night average systolic pressure and the night average diastolic pressure were higher than that of the control group (P > 0.05). The difference of daytime diastolic pressure and night diastolic pressure of the control group was higher than that of OHT group (P > 0.05). Most children of the OTH group had non-dipper blood pressure type (72.5%), while most children of the control group had a dipper blood pressure type (55.0%). In addition, the time domain SDNN and SDANN in the OHT group were higher than those in the control group (P < 0.01). And, the indicators including TP, ULF, VLF, and LF/HF were higher in the OHT group, when compared with control group (P < 0.01). Besides, in terms of subgroup analysis within the OHT group, the age difference between boys and girls was not statistically significant (P > 0.05). When compared with grils, the time domain SDNN increased (P = 0.003), and the frequency index TP, ULF, and VLF increased in boy group (P < 0.05).ConclusionOHT Children’s autonomic nervous system showed dysfunction, and differences of systolic blood pressure between day and night were much lower than those of healthy children, and the main blood type was non-dipper blood pressure with circadian rhythm disappearing.

Home blood pressure monitoring using an electronic sphygmomanometer Acceptability, comparability and effects on the diagnosis and management of hypertension

Background: The recent development of reliable, economic, semiautomatic electronic blood pressure monitors provides an opportunity for GPs to monitor blood pressure outside the surgery environment. Because office measurements fail to define those patients with whitecoat hypertension and whitecoat effect, we are currently overdiagnosing hypertension.Objectives: To examine the comparability of the electronic sphygmomanometer (Omron HEM 705CP) with a standard mercury sphygmomanometer. To assess the prevalence of whitecoat hypertension and whitecoat effect in general practice and the effect of these diagnoses on the subsequent management of hypertension.Method: 87 consecutive patients with established or suspected hypertension were recruited over a six-month period. Average home readings were compared with average office readings to determine prevalence of whitecoat hypertension and whitecoat effect. Simultaneous electronic and mercury readings allowed evaluation of the electronic monitor throughout the study period. A patient questionnaire assessed the acceptability of the monitor and home blood pressure measurement. A GP questionnaire assessed the effect on blood pressure management.Results: Patients were generally quite happy with the monitor. The simultaneous electronic/mercury readings showed good comparability. Home blood pressure was significantly lower than office blood pressure. 24% (21 patients) had a significant element of whitecoat effect (>20/10 mmHg) and 6% (5 patients) had whitecoat hypertension. At the end of the study, 37% (32 patients) were on less medications than if they had been managed based on office blood pressure.Conclusions: This study indicates that home blood pressure monitoring can distinguish sustained hypertension from office hypertension and thus reduce overdiagnosis and overtreatment of hypertension.