Abstract This study aims to improve how heat and mass move in systems that use viscoelastic nanofluids under magnetic fields. These systems are commonly used in industries like biotechnology, energy, and medical devices. The significance of this work lies in exploring the steady flow of magnetohydrodynamic (MHD) Casson nanofluids, incorporating the Buongiorno nanofluid model and swimming microorganisms. This research seeks to deepen the understanding of complex fluid behaviors by examining the effects of thermal radiation and chemical diffusion under thermal and solutal convective boundary conditions. The governing equations, which are inherently nonlinear due to the presence of multiple physical effects, are converted from two-dimensional partial differential equations (PDEs) to ordinary differential equations (ODEs) using a similarity transformation. A semi-analytical solution is derived using the collocation pseudo-spectral method within the MAPLE computational software. The study investigates how factors like Casson and magnetic parameters, Eckert number, Brownian motion, and thermophoresis affect the flow rate, temperature distribution, species concentration, and microorganism motility. These results are validated by comparing them with established benchmarks. The key findings reveal a pronounced oscillatory behavior in the temperature profile at higher Eckert number values, while increased Brownian motion and thermophoresis lead to greater nanoparticle dispersion near the disk surface. Higher Lewis and Peclet numbers lead to increased microorganism concentration, demonstrating stronger convective and advective effects. These insights are vital for optimizing drag force, thermal gradients, and mass transfer in engineering applications that involve rotating disks and magnetic fields.