Selected Body Parts Research Articles

Parameterization of a biomechanical collision model for safe human‐robot collaboration

AbstractThe Fraunhofer IFF has developed a patented model (Fast Response Model = FRM) for safe Human‐Robot Collaboration (HRC), enabling the calculation of reaction forces in the event of a collision between a contact body of any shape and a selected human body part. This paper specifically focuses on adapting the model for five selected body locations on the hand‐arm system. A comparative analysis is conducted, contrasting the results obtained from the FRM with those derived from existing finite element reference models to assess the plausibility of the model's system response. Additionally, computational times of the model are compared with those of established models for simulation‐based risk evaluation of robot collisions. The model's quality is validated using a validation geometry. Results indicate that depending on the selected body part, end effector geometry, and given displacement, similar results to a finite element model can be obtained. However, simulations with the FRM are less time‐consuming. In the future, the data‐driven model could be transformed into a real‐time model using machine learning techniques.

Correlation dimension and entropy in the assessment of sex differences based on human gait data.

It is proved that there are differences between gait performed by females and males, which appear in movements of selected body parts. Despite numerous state-of-the-art studies related to the discriminative analysis of motion capture data, the question of whether measures of signal complexity and uncertainty can extract valuable features for the problem of sex distinction still remains open. It is the subject of the paper. Correlation dimension, as well as approximate and sample entropies, are selected to describe motion data. In the numerical experiments, the collected dataset with 884 samples of 25 females and 30 males was used. The measurements took place in the Human Motion Laboratory (HML), equipped with a highly precise motion capture system. Two variants of data representation were investigated-time series that contain joint rotations of taken skeleton model as well as positions of the markers attached to the human body. Finally, a comparative analysis between the populations of females and males using descriptive statistics, non-parametric estimation, and statistical hypotheses verification was carried out. There are statistically significant sex differences extracted by the taken measures. In general, the movements of lower limbs result in greater values of correlation dimension and entropies for females, while selected upper body parts play a similar role for males. The dissimilarities are mainly observed in hip, ankle, shoulder, and head movements. Correlation dimension and entropy measures provide robust and explainable features of motion capture data with a valuable description of the human locomotion system. Thus, beyond the importance of discovered differences between females and males, their interpretation and understanding are also known.

Gorczyciana sulawesica-a remarkable new plant bug genus and species(Heteroptera: Miridae: Cylapinae) from Indonesia.

Gorczyciana gen. nov., a new remarkable genus of the subfamily Cylapinae, tribe Fulviini, is described from Indonesia. The new species Gorczyciana sulawesica sp. nov. from Sulawesi is documented with photographic images, SEM micrographs of the selected body parts, and female genitalic structures. Identification key to the cylapine tribes and genera of the tribe Fulviini of the Oriental Region is also given.

First insight in element localisation in different body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS

Acanthocephalans, intestinal parasites of vertebrates, are characterised by orders of magnitude higher metal accumulation than free-living organisms, but the mechanism of such effective metal accumulation is still unknown. The aim of our study was to gain new insights into the high-resolution localization of elements in the bodies of acanthocephalans, thus taking an initial step towards elucidating metal uptake and accumulation in organisms under real environmental conditions. For the first time, nanoscale secondary ion mass spectrometry (NanoSIMS) was used for high-resolution mapping of 12 elements (C, Ca, Cu, Fe, N, Na, O, P, Pb, S, Se, and Tl) in three selected body parts (trunk spines, inner part of the proboscis receptacle and inner surface of the tegument) of Dentitruncus truttae, a parasite of brown trout (Salmo trutta) from the Krka River in Croatia. In addition, the same body parts were examined using transmission electron microscopy (TEM) and correlated with NanoSIMS images. Metal concentrations determined using HR ICP-MS confirmed higher accumulation in D. truttae than in the fish intestine. The chemical composition of the acanthocephalan body showed the highest density of C, Ca, N, Na, O, S, as important and constitutive elements in living cells in all studied structures, while Fe was predominant among trace elements. In general, higher element density was found in trunk spines and tegument, as body structures responsible for substance absorption in parasites. The results obtained with NanoSIMS and TEM-NanoSIMS correlative imaging represent pilot data for mapping of elements at nanoscale resolution in the ultrastructure of various body parts of acanthocephalans and generally provide a contribution for further application of this technique in all parasite species.

Thermography as a tool to assess training effects in military working dogs

Military working dogs are exposed to high levels of physical load during their work, although this is not always evident from their behaviour. This workload causes various physiological changes, including variation in the temperature of the affected body parts. In this preliminary study, we investigated whether thermal changes following the daily work routine of military dogs are detectable by infrared thermography (IRT). The experiment was carried out on eight male German and Belgian shepherd patrol guard dogs performing two training activities, obedience and defence. The surface temperature (Ts) of 12 selected body parts on both body sides was measured with the IRT camera 5 min before, 5 min after and 30 min after training. As predicted, there was a greater increase in Ts (mean of all measured body parts) after defence than after obedience, 5 min (by 1.24 vs. 0.60 °C, P < 0.001) and 30 min after activity (by 0.90vs. 0.57 °C, P < 0.01) compared to pre-activity levels. These findings indicate that defence is more physically demanding than obedience activity. Considering the activities separately, obedience increased Ts 5 min after the activity only in the trunk (P < 0.001) but not in the limbs, whereas defence increased it in all measured body parts (P < 0.001). Ts 30 min after obedience decreased to the pre-activity level in parts at trunk, while in distal parts of the limbs Ts remained elevated. The prolonged elevation in Ts of the limbs after both activities indicates heat flow from the core to the periphery as a thermoregulatory mechanism. The current study suggests that IRT may be a useful tool for assessing physical workload in different body parts of dogs.

The impact of trade on animal exploitation in Rome during the Roman period: The evidence from zooarchaeological analysis

In this paper some results derived from zooarchaeological studies carried out in Rome are presented and discussed in detail; these provide evidence of the trade and circulation of animals, meat and other animal products to and from the ancient city. Over the past forty years a large number of animal remain assemblages from the urban area of Rome have come to light and have been studied, producing a large amount of information on several aspects of animal consumption and exploitation in ancient times. The discovery of exotic or rare animal species, the change in the frequency of selected body parts of domesticated animals destined to the urban market, and the identification of specific processing methods linked to the circulation of animal products are here discussed in relation to evidence from other resources, in order to outline the nature of animal and animal product trade during the Roman period.

Gender wise consideration of Anguli Pramana in the present era

Introduction: Anguli pramana is a unique concept in Ayurved. It was stated by acharyas at times when there was no any sort of standardised unit of measurements. Acharyas have mentioned Anguli pramana for various parts of the body. In respect of male and female gender, no separate anthropometric values found to be mentioned. Hence, the present study aimed at gender wise consideration of Anguli pramana. Method: After seeking ethical permission, 770 healthy participants of both gender between age group 18-50 years in Ahmednagar and Nashik region were selected for the study. Measurements were taken for selected body parts. The middle finger width of the right hand at proximal interphalangeal joint was used as a unit for Anguli pramana. Collected data treated with the Student’s t test for inference. Result: Measured values for selected parameters when compared with the standard values stated by acharya in both genders found statistically different except for a few i.e., Pad parinah and Pad vistar. Discussion: Variations in Anguli pramana may be due to evolutionary changes and lifestyle in the present era. The present study concluded that Anguli pramana is not valid in the present era. Its validity was checked according to gender separately. Though the findings are confined to the selected sample size and selected parameters, it could further be extended to arrive at generalization.

Quantitative Investigation of Body Part Selection for Data-Driven Personal Overall Thermal Preference Prediction

Personal thermal preference information can help to create a building environment that satisfies all staff, instead of an environment that only satisfies most people, to enhance personal thermal comfort. Research has shown that thermal preference can be predicted using parameters that are based on various local body parts, but the selected body parts are often different. Using too many body parts for the measurements leads to high costs, while using too few body parts results in large errors. In this study, 19 adult subjects (8 females and 11 males) were recruited, their overall and local thermal preferences were surveyed, and the skin temperature of seven body parts were measured. A machine learning algorithm, random forest, was employed to analyse the contributions of different body parts. Three criteria (the best combination, fewest combination, and common combinations) were employed to select body parts to use to establish thermal preference models for individuals and groups. The results show that the prediction power of these combinations reached 0.91 ± 0.07 (accuracy), 0.75 ± 0.16 (Cohen’s kappa), and 0.87 ± 0.09 (AUC) when using 2–8 body parts. The common combinations are recommended for their balance of their prediction power and the number of local body parts involved. This study offers a reference for efficient and economic measurements for thermal comfort research in building environments.

Preliminary Study of Slaughter Value and Meat Characteristics of 18 Months Ostrich Reared in Hungary

The study aimed to evaluate the slaughter value and meat characteristics of ten ostrich females reared and slaughtered at the age of 18 months in Hungary. The ratio of selected body parts, the main organs and the lean meat parts were examined. The nutritive composition, the colour, the technological and organoleptic characteristics of five valuable meat parts (outside strip—M. flexor cruris lateralis, oyster—M. iliofemoralis externus, tip—M. femorotibialis medius, outside leg—M. gastrocnemius pars externa, medal—M. ambiens) and the amino acid, fatty acid and mineral composition of outside strip (M. flexor cruris lateralis) were also evaluated. The ratio of body parts and the main organs as the percentage of live weight, and the lean meat part as the percentage of carcass weight showed 16.74 ± 0.01%, 6.16 ± 0.01% and 57.29 ± 0.59%, respectively. The dry matter content of the examined valuable meat parts ranged between 24.89 ± 0.08 and 26.23 ± 0.13%, the protein ratio took on values between 18.40 ± 0.09 and 20.62 ± 0.16%, the fat content showed values between 2.36 ± 0.07 and 4.50 ± 1.09% and the hydroxyproline content ranged between 0.01 ± 0.001 and 0.08 ± 0.001%. The amino acid content of the outside strip showed a range between 0.15 and 3.33%. The ratio of SFA, MUFA and PUFA was 35.10 ± 0.53, 37.37 ± 1.52 and 27.54 ± 1.01. The n-6/n-3 ratio showed 3.91 ± 0.43 and the SFA/UFA ratio was 0.54 ± 0.02. Among the examined minerals, the content of Ca, K, Mg, Na and P was the highest in the meat. In the case of the colour, regarding L* value, we could reveal no significant difference between the examined meat parts. For a* and b* values, the outside leg had the lowest data of all. We could not reveal a significant difference between the pH values of the meat parts. Regarding technological parameters, meat differed only in thawing loss. The significantly lowest thawing loss could be detected in the outside leg (2.72 ± 0.01%) and in the medal (2.32 ± 0.01%). The results of the organoleptic evaluation showed that the outside strip and the tip had the best flavour and tenderness. In comparison with the younger birds (10–14 months of age) in the literature, the 18-month-old ostriches in our study showed similar or slightly lower slaughter weight, skin weight and head ratio, greater liver weight, lighter meat, lower protein and higher fat content, higher essential amino acid and lower non-essential amino acid content and higher SFA content in some cases. However, data on nutrition and population size were not always available. In comparison with other ratites (emu and rhea), ostrich meat has lower dry matter and protein, but higher fat, SFA, MUFA and PUFA content and lower n-6/n-3 ratio.

Analysis of Age-Based Bone Mineral Density in the Korean Adult Population Using Dual-Energy X-ray Absorptiometry

Dual-energy X-ray absorptiometry (DEXA) provides a reliable and accurate measurement of bone density and bone mineral composition. This research examined the composition and bone density (bone mineral composition and bone mineral density; BMD) of the whole body and selected body parts using DEXA. The participants were 240 healthy adult men and women who were divided into three groups based on age. The total BMD of women amounted to an average of 1.14 g/cm2 for those aged 20–39 years, 1.14 g/cm2 for those aged 40–59 years, and 0.98 g/cm2 for those aged 60–73 years. For men, the average BMD was 1.25 g/cm2 for those aged 20–39 years, 1.20 g/cm2 for those aged 40–59 years, and 1.17 g g/cm2 for those aged 60–73 years. The decrease in age-specific BMD was shown to have a correlation with both age and body mass index, and it is determined that exercising on a regular basis can prevent a reduction in BMD by maintaining appropriate muscle mass.

Multiclass Classification Based on Combined Motor Imageries.

Motor imagery (MI) allows the design of self-paced brain–computer interfaces (BCIs), which can potentially afford an intuitive and continuous interaction. However, the implementation of non-invasive MI-based BCIs with more than three commands is still a difficult task. First, the number of MIs for decoding different actions is limited by the constraint of maintaining an adequate spacing among the corresponding sources, since the electroencephalography (EEG) activity from near regions may add up. Second, EEG generates a rather noisy image of brain activity, which results in a poor classification performance. Here, we propose a solution to address the limitation of identifiable motor activities by using combined MIs (i.e., MIs involving 2 or more body parts at the same time). And we propose two new multilabel uses of the Common Spatial Pattern (CSP) algorithm to optimize the signal-to-noise ratio, namely MC2CMI and MC2SMI approaches. We recorded EEG signals from seven healthy subjects during an 8-class EEG experiment including the rest condition and all possible combinations using the left hand, right hand, and feet. The proposed multilabel approaches convert the original 8-class problem into a set of three binary problems to facilitate the use of the CSP algorithm. In the case of the MC2CMI method, each binary problem groups together in one class all the MIs engaging one of the three selected body parts, while the rest of MIs that do not engage the same body part are grouped together in the second class. In this way, for each binary problem, the CSP algorithm produces features to determine if the specific body part is engaged in the task or not. Finally, three sets of features are merged together to predict the user intention by applying an 8-class linear discriminant analysis. The MC2SMI method is quite similar, the only difference is that any of the combined MIs is considered during the training phase, which drastically accelerates the calibration time. For all subjects, both the MC2CMI and the MC2SMI approaches reached a higher accuracy than the classic pair-wise (PW) and one-vs.-all (OVA) methods. Our results show that, when brain activity is properly modulated, multilabel approaches represent a very interesting solution to increase the number of commands, and thus to provide a better interaction.

Splitting bodies – A close-up study of a South Swedish bog deposition from the Pre-Roman Iron Age

A detailed taphonomic study of human and faunal remains recovered in a wetland in Southern Sweden is presented with specific focus on taphonomy, chronology and the possible practices associated with the handling of the remains of humans and animals, especially dogs. The finds from the wetland include remains of seven humans together with 21 dogs and skeletal remains of additionally 21 individuals of red fox, cattle, sheep, horse, red deer, and pig deposited in three pits located in different parts of the bog. Remains of five adults, three of which are female and of different age were identified in two of the pits as well as two children. The adults are represented by selected body parts only while the two children exhibit a more complete anatomical representation. Radiocarbon dating place the depositional activities mainly in the latest Bronze Age and Pre-Roman Iron Age (c.750–250 BCE), however, probably mainly in the latter part of the interval. Separate depositional events occurred in the 7th–8th century and 9th-10th centuries CE.There are taphonomic indications of pre–depositional handling of the bodies. Parts of human bodies were deposited when in some cases the bones were dry and in other cases when soft tissue still was present. The handling of the human body parts may be connected to a “pars pro toto” concept, including a ritual practice of individual bone circulation before deposition. Selective recovery needs to be considered but that or secondary movement of elements after deposition due to water movement cannot explain the patterns observed in the anatomical representation. A minimum of 17 dogs were deposited in the largest pit, probably as almost complete bodies. Two thirds of the recovered dog crania show evidence of healed traumatic injuries showing they were handled in a harsh manner in encounters with the humans. Other animals in the deposition were represented by selected parts of the bodies deposited both in a fresh state, probably as meat cuts, and by isolated elements. As a case study, the composition and character of the finds at Östra Vemmerlöv have to be considered unique in providing intriguing insights into the Latest Bronze Age/Earliest Iron Age wetland depositions, though parallels are found both locally and in a larger area, e.g. present day Denmark and Germany.

Frequency and intensity of pain symptoms detected during classic massage sessions of selected body parts in purebred Arabian racing horses

ABSTRACT We analysed the frequency of symptoms and degree of muscle pain in selected body parts of racing horses assessed during classic massage sessions. The influence of horse’s sex on obtained results was considered. The potential for the early determination of pain in horses by analysing their behaviour and cardiac parameters during a massage session was also evaluated. The study was conducted on 20 three-year-old purebred Arabian horses during one racing season. In the racing season, cyclic classic massage sessions [...]

Josef Mengele's research program on noma in Auschwitz

Noma research protocol was conducted in the Auschwitz Birkernau camp by the infamous (Schutzstaffel) SS doctor Josef Mengele who was known as “the Angel of Death” in close collaboration with researchers at the Kaiser-Wilhelm Institute in Berlin Crimes. Mengele who held the post of camp doctor in the “Gypsy camp” saw an opportunity to set up a research program on gypsy children called “Nomaprojekt”. The purpose of this program was to study the causes of noma and to find treatment methods. The experimental treatment protocol consisted of administering a combination of sulfanilamido-ethyl thiodiazole and nicotinic acid. Heads and selected body parts of children suffering from noma were prepared in formaldehyde jars and sent to researchers at the prestigious Kaiser-Wilhelm Institute for Anthropology, Human Heredity and Eugenics in Berlin-Dahlem, but also to the SS Medical Academy in Graz to carry out further examinations. The experimental protocol for the treatment of noma among Gypsy children is one of the most horrific crimes that Dr. Josef Mengele committed in the Auschwitz camp.

Body cathexis and fit preferences of young South African women of different body shapes and ethnicity

ABSTRACT Young African (109) and Caucasian (125) women of different body shapes self-reported their perceptions of an ideal figure, body cathexis and fit preferences through a questionnaire. The hourglass shape was perceived by all shapes as the ideal and was further confirmed by a chi-square test (p > 0.05), which showed no association between participants’ body shapes and perceived ideal figure. All the shapes reported dissatisfaction with their mid and lower body parts. Close fit was preferred by most shapes, except Caucasian rectangle that preferred semi-fitted apparel at the abdomen. The results clearly pointed to the influence of body shape and ethnicity on the satisfaction with selected body parts. However, body shape did not seem to influence apparel fit preference. Apparel manufacturers need to better understand the interrelationship between body shapes, body cathexis and fit preferences to adapt manufacturing and marketing strategies to improve fit needs of their target markets.

Hierarchical Soft Quantization for Skeleton-Based Human Action Recognition

In daily life, human beings rely on hands and body parts to complete particular actions cooperatively. These selected body parts and their cooperative relationships are essential cues to distinguish these actions. However, most existing action recognition methods, which try to model the body appearance or spatial relations in skeleton sequences, often ignore the essential cooperation relationship among joints. Differently, in this paper, we propose a spatio-temporal hierarchical soft quantization method to extract the congenerous motion features, which reflect the cooperation relations among joints and body parts. Specifically, we design a hierarchical network with multiple soft quantization layers to extract congenerous features. The hierarchical network not only models the spatial hierarchy of skeleton structure for joint, part, and body, but also extracts the temporal hierarchy with sliding windows for frame, fragment, and sequence. Moreover, the features in each layer are visually explainable, which reflect the cooperation among body parts. The trainable parameters in the network are also significantly reduced, which reduces computational cost. Extensive experiments conducted on four benchmarks demonstrate that our method can provide competitive results compared with state-of-the-arts. The visualized congenerous features also validate that our approach can effectively perceive the essential cooperation relations.

Body temperature of horses spending time in paddocks in various weather conditions during the summer

The aim of the study was to determine the internal and surface temperature of selected body parts of horses during their stay in paddocks in various weather conditions in the summer. The study was conducted on 10 adult thoroughbred mares. The experiment was carried out on horses spending time in the paddock in four eight-hour stages with differing weather conditions in summer (sunny, cloudy, rainy and windy). Measurements of internal temperature (Veterinär SC 12 veterinary thermometer) and surface temperature of the head, rib area and croup (Thermal Imagers Ti9 FLUKE infrared camera and SmartView 4.1 software) were carried out at rest and again after four and eight hours in the paddock. It was concluded that the time horses spend in the paddock in summer should depend on weather conditions. Many hours spent outside the stable during sunny weather accompanied by high air temperature can contribute to overheating. On the other hand, rain and wind can cause hypothermia. Therefore, it is worth considering leaving horses in the stable or limiting their stay in the paddock to no more than four hours.

Distribution of Superficial Body Temperature in Horses Ridden by Two Riders with Varied Body Weights

Simple SummaryLeisure horse riding is becoming an increasingly popular activity, but often the regard for the welfare of recreational horses is insufficient. Most often, this situation is associated with workload or insufficient knowledge of the owners. Another problem may be overloading the animals with excessive weight of the rider, because the problem of obesity affects more and more societies. Carrying heavy loads significantly reduces horse comfort and affects movement mechanics. Prolonged use of the horse in this way may translate into health and lameness issues in the back and limbs. There are currently no strict weight limits for riders. However, we believe that horse users should pay more attention to this problem. As shown in this study, a horse’s load above 20% of his body weight, even with little effort, affects changes in surface temperature and the activity of the autonomic nervous system.It was assumed that a horse with its rider body weight found in the upper limit may negatively impact the horse’s welfare. The objective of this paper was to analyze the differences in body temperature and selected heart rate parameters in horses in response to physical exercise accompanied by various rider’s body weight loads. The study was carried out on 12 leisure, 10–15-year-old warmblood geldings. The horses were ridden by two equally qualified riders whose body weights were about 20% and 10% of the average body weight (BW) of the animals (about 470 kg). Each rider rode each of the 12 horses for 13 min walking and 20 min of trotting. Images of the horse at rest, after physical exercise directly after unsaddling, and during the recovery phase (10 min after unsaddling) were taken with an infrared thermography camera. For analysis, the temperatures of selected body parts were measured on the surface of the head, neck, front, middle, and back (croup) parts of the trunk, forelimb, and hind limb. Immediately after the infrared thermography images were taken, the rectal temperature of the horse was measured. The heart rate parameters were measured at rest for 10 min directly before, during, and 10 min following the end of a training session. A multivariate analysis of variance (ANOVA) for repeated measurements was performed. Statistical significance was accepted for p < 0.05. A rider BW load on a horse of approximately 20% of the horse’s BW led to a substantial increase in the superficial temperatures of the neck, front, middle, and back parts of the trunk in relation to these body parts’ average temperatures when the load was about 10% BW. The head and limb average temperatures were not significantly affected by the load of the exercised horse. A horse’s load above 20% of his body weight, even with little effort, affects changes in surface temperature and the activity of the autonomic nervous system.

Metaphoric Conceptualization of Love Pain or Suffering in Turkish Songs through Natural Phenomena and Natural Disasters

ABSTRACTTraditional Turkish love is identified with suffering. This study investigates how suffering in love is metaphorically conceptualized in Turkish via natural phenomena and disasters. Based on figurative expressions in sad love songs of Arabesque and Art music genres, the study reveals that love pain/suffering is expressed through three salient metaphors: 1) love paın/sufferıng ıs natural phenomena, 2) love paın/sufferıng ıs natural dısasters, and 3) the overarching metaphor (the suffering) lover’s body ıs a landscape (affected by weather conditions or natural disasters). It was unearthed from metaphoric expressions in lyrics that as part of a cultural scenario, natural phenomena and disasters are profiled as internalized forces culturally imagined to directly affect vital body parts to express the emotional distress or anguish of the Turkish lover. The overarching metaphor lover’s body ıs a landscape is a manifestation of conceptual integration producing a novel structure in which natural phenomena appear to occur in or to culturally selected body parts or organs. The study makes a distinction between the three metaphors above about body parts and the well-known love ıs a natural force (which affects the WHOLE body as if it were an object being swept away or blown about, etc.)

Comparative analysis of some metallic elements in selected body part of some fishes (Mormyrus rume and Heterobranchus longifilis) captured from Igbokoda River, Okitipupa, Ondo State, Nigeria

Fishes are important for the maintenance and growth of living things, particularly human beings. Due to the nutritional value of fish, people are encouraged to include it into their diet. Fish is consumed in almost every house in Igbokoda, Nigeria and in the surrounding communities and is typically consumed in either (smoked, fried, cooked) condition. When these fish are polluted with heavy metals, they bioaccumulate and cause certain human organs to malfunction. The content of toxic elements in two different species (Mormyrus rume and Heterobranchus longifilis) of fish captured from the Igbokoda River of Ilaje was analyzed using Energy Dispersive X-ray Fluorescence (EDXRF). The result obtained showed that the mean values of all the elements were higher than the recommended FAO/WHO and International Atomic Energy Agency (IAEA) 407 standards, except for K, Ca, Sr and As, which were lower than the IAEA 407. Generally, the concentration of elements in muscle and head was higher than that of tail for each species for both M. rume and H. longifilis. The target hazard quotient (THQ) of each element through the consumption of Igbokoda fish for both adults and children increases in this order Mn > Cu > Ni > Zn > Fe > As > Pb and Mn > Cu > Ni > Zn > Fe > As > Pb for M. rume and H. longifilis respectively.