The political landscape of Italy experienced a seismic shift in the September 2022 elections, marking a watershed moment. Giorgia Meloni, the leader of the populist radical right party (PRRP) Brothers of Italy, was elected as Italy’s head of government. This event highlighted a significant development in the country’s gender politics, reflecting the ascent of PRRPs across Europe, marked by their reactionary politics, emphasis on traditional values and staunch opposition to gender equality. This study focuses on the dynamics between PRRPs (Brothers of Italy and the League) and the anti-gender movement in Italy. Through a qualitative content analysis of the primary sources of political parties and movements, the research explores their symbiotic relationship, the linkage and adjustment mechanisms employed by both actors, and the political outcomes of their interactions, offering insights into the mobilization of anti-gender actors and their alliance with PRRPs. It contributes to understanding how the organized opposition to gender equality is mainstreamed within political spheres, especially in contexts where right-wing populists hold power.