The study of biotic nomenclature has resulted from gene expression profiling using integrated image analysis techniques. The study emphasized in this paper confers Modified Intuitionistic Fuzzy Clustering Method (MIFCM) for spot segmentation of microarray image. In order to achieve the basic condition of intuitionism, a generator using Intuitionistic Fuzzy set (IFS) is used. MIFCM approach considers degree of uncertainty, value of association and value of non-association to determine similarity between the microarray spot data points which further helps in clustering. For non-association value calculation, Sugeno's [47] and Yager's [30][44] fuzzy complement generator [49] with intuitionism approach is proposed in this work. To achieve elementary level of intuitionism [49], MIFCM is integrated with Hausdorff distance metrics [43]. The Hausdorff distance metrics is used to quantify the distance metrics betwixt microarray image pixel and cluster centers. The study discusses the constraints of the evolutionary Fuzzy C-Means (FCM) method [4] and adoption of new fuzzy complement generator which overcomes limitations to increase the clustering efficiency. Further, the paper presents detailed methodology of calculating the parameters required to convert crisp spot data of microarray image into fuzzy set which will be input to the next stage of kernel induced clustering using MIFCM technique.