Garden Cress Seeds Research Articles

Crude Garden Cress Seed Oil (Lepidium sativum Linn.) Enhances Post-Thawed Boar Sperm Quality

This study aimed to examine the effects of crude garden cress seed oil (CGCSO) on frozen–thawed boar sperm qualities. Semen ejaculates (n = 12) were collected and further divided into six equal aliquots based on CGCSO concentrations (0, 0.5, 1, 1.5, 2, and 2.5% v/v) in the freezing extender. Semen samples were processed and cryopreserved utilizing the traditional liquid nitrogen vapor technique. Subsequently, semen samples were thawed in a thermos with warm water at 50 °C for 12 s and evaluated for sperm morphology using scanning electron microscopy, sperm motility using a CASA, sperm viability, acrosome integrity, mitochondrial function, MDA level, total antioxidant capacity (TAC), glutathione peroxidase (GSH-Px), and catalase (CAT) activity. The results indicated that 1% CGCSO resulted in superior post-thaw sperm characteristics, including enhanced sperm morphology, motility, viability, acrosome integrity, and mitochondrial function. Particularly, the total motile sperm increased by 16.5%, progressive motile sperm increased by 13.0%, viability improved by 15.1%, acrosome integrity increased by 14%, and mitochondrial function improved by 14.1% compared to the control group. CGCSO treatment at 1% and 1.5% exhibited the lowest level of MDA (45.73 ± 11.2 and 45.73 ± 11.3 µmol/L, respectively) compared to the other groups. The CGCSO-supplemented groups showed higher values of TAC, GSH-Px, and CAT than the control group but not significantly.

Isolation and Selection of Protein-Rich Mutants of Chlorella vulgaris by Fluorescence-Activated Cell Sorting with Enhanced Biostimulant Activity to Germinate Garden Cress Seeds.

Microalgae are a promising feedstock with proven biostimulant activity that is enhanced by their biochemical components (e.g., amino acids and phytohormones), which turns them into an appealing feedstock to reduce the use of fertilisers in agriculture and improve crop productivity and resilience. Thus, this work aimed to isolate protein-rich microalgal mutants with increased biostimulant activity. Random mutagenesis was performed with Chlorella vulgaris, and a selection of protein-rich mutants were sorted through fluorescence-activated cell sorting (FACS), resulting in the isolation of 17 protein-rich mutant strains with protein contents 19-34% higher than that of the wildtype (WT). Furthermore, mutant F4 displayed a 38%, 22% and 62% higher biomass productivity, growth rate and chlorophyll content, respectively. This mutant was then scaled up to a 7 L benchtop reactor to produce biomass and evaluate the biostimulant potential of this novel strain towards garden cress seeds. Compared to water (control), the germination index and the relative total growth increased by 7% and 19%, respectively, after the application of 0.1 g L-1 of this bioproduct, which highlights its biostimulant potential.

The Role of Lepidium Sativum as Free Radical Scavenger in Laboratory Mice

Background: Lepidium sativum is a by-product remaining after the oil is extracted from Garden cress seeds. This herb is considered one of the popular medicinal herbs used in Arabian countries and has traditionally been used to control many clinical problems. The present study aims to uncover these seeds' free radical-scavenging properties and their Role in improving the testicular tissues and seminal properties. Material and Methods: Thirty-two sexually mature mice were used, and the treated groups were divided into three groups; the first one (G1) was treated with 0.1 ml of GC seed extraction in a dose of 5mg/kg BW, the second one(G2) was treated with 0.1 ml sodium nitrate as oxidant, and the last treated group (G3) was treated with 0.1 ml of GC seed extraction after one hour of injection of 0.1 ml sodium nitrate. Results: The results showed improvement of the testicular tissue and a significant elevation in sperm viability, in addition to significant enhancements of RBC count and Hb concentration. By its Role as a free radical scavenger, sodium nitrate removed the negative effect on blood parameter values.

Dietary garden cress (Lepidium sativum) seeds mitigate the effect of aflatoxin B1 contamination on growth, antioxidant status, AFB1 residues, immune response, and tissue architecture of Oreochromis niloticus

The contamination of fish diets with aflatoxin B1 (AFB1) poses a significant hazard to the aquaculture sector. This study investigates the efficacy of supplementing Nile tilapia (Oreochromis niloticus) diets with garden cress (Lepidium sativum) seeds (GCS) to mitigate the adverse effects of AFB1. For this purpose, a factorial design was utilized, consisting of a 2×3 arrangement of treatments. This included two contamination levels of AFB1: (0 and 2 mg kg−1 diet). Within each contamination level, three dietary levels of GCS were used (0 %, 1 %, and 2 %). The study involved 270 healthy O. niloticus with an average weight of 25.14 ± 0.39 g (45 fish/group and 15 fish/replicate). The duration of the trial was 60 days. The study evaluated growth performance, hematological indices, hepatorenal function, immune and antioxidant indicators, histomorphology of organs, AFB1 residues in muscles, and fish resistance to Aeromonas hydrophila. The results indicated that GCS dietary supplementation significantly counteracted AFB1-induced growth retardation and reduced fish survival. Additionally, GCS effectively reversed the anemia, leukopenia, hypoproteinemia, hypoalbuminemia, and hypoglobulinemia that were produced by AFB1. Moreover, GCS dietary supplementation significantly suppressed the AFB1-induced increase in serum bilirubin, hepatic enzymes, kidney damage products, cortisol, and glucose. Additionally, the inclusion of GCS in the fish's diet effectively eliminated the accumulation of AFB1 in their muscles. Moreover, GCS supplementation demonstrated significant restoration of depleted antioxidant and innate immune components induced by AFB1. Furthermore, GCS significantly reduced the degenerative changes in renal, hepatic, splenic, and small intestine tissues caused by AFB1. Fish fed a GCS-fortified diet and exposed to AFB1 displayed restored disease resistance when challenged with A. hydrophila. In conclusion, supplementing Nile tilapia diets with GCS at 2 %, even in the presence the AFB1 contamination, significantly enhanced the fish growth, improved health, and increased disease resistance while reducing AFB1 residues, thereby benefiting consumer health.

Garden cress seeds: a review on nutritional composition, therapeutic potential, and industrial utilization.

The growing preference for natural remedies has resulted in increased use of medicinal plants. One of the most significant and varied plants is garden cress (Lepidium sativum), which has large concentrations of proteins, fatty acids, minerals, and vitamins. It also contains a wide range of bioactive components, including kaempferol glucuronide, gallic acid, protocatechuic acid, coumaric acid, caffeic acid, terpenes, glucosinolates, and many more. These substances, which include antioxidant, thermogenic, depurative, ophthalmic, antiscorbutic, antianemic, diuretic, tonic, laxative, galactogogue, aphrodisiac, rubefacient, and emmengogue qualities, add to the medicinal and functional potential of garden cress. An extensive summary of the phytochemical profile and biological activity of garden cress seeds is the main goal of this review. Research showed that garden cress is one of the world's most underutilized crops, even with its nutritional and functional profile. Consequently, the goal of this review is to highlight the chemical and nutritional makeup of Lepidium sativum while paying particular attention to its bioactive profile, various health claims, therapeutic benefits, and industrial applications.

Auxin production and plant growth promotion by Microbacterium albopurpureum sp. nov. from the rhizoplane of leafless Chiloschista parishii Seidenf. orchid.

The strains of the genus Microbacterium, with more than 150 species, inhabit diverse environments; plant-associated bacteria reveal their plant growth-promoting activities due to a number of beneficial characteristics. Through the performance of diverse techniques and methods, including isolation of a novel Microbacterium strain from the aerial roots of leafless epiphytic orchid, Chiloschista parishii Seidenf., its morphological and biochemical characterization, chemotaxonomy, phylogenetic and genome analysis, as well as bioassays and estimation of its auxin production capacity, a novel strain of ET2T is described. Despite that it shared 16S rRNA gene sequence similarity of 99.79% with Microbacterium kunmingense JXJ CY 27-2T, so they formed a monophyletic group on phylogenetic trees, the two strains showed clear divergence of their genome sequences. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values of ET2T differed greatly from phylogenetically close JXJ CY 27-2T. Based on the differences being below the threshold for species similarity, together with the unique chemotaxonomic characteristics, strain ET2T represents a novel species of the genus Microbacterium. Several genes, putatively involved in auxin biosynthesis were predicted. This strain revealed obvious plant growth-promoting activities, including diazotrophy and biosynthesis of tryptophan-dependent auxins (indole-3-acetic and indole-3-pyruvic acids). Microbial auxins directly stimulated the rhizogenesis, so that the ET2T-inoculated seeds of wheat, cucumber and garden cress showed evident promotion in their growth and development, both under optimal and under cold stress conditions. Based on phenotypic, chemotypic and genotypic evidences, the strain ET2T belongs to the genus Microbacterium, order Micrococcales, class Actinomycetes, and it represents a novel species, for which the name Microbacterium albopurpureum sp. nov. is proposed, with strain ET2T (VKPM Ac-2212, VKM Ас-2998) as the type strain.

Impact of Pretreatment of Garden Cress Seed on Rheological Properties for Bread Dough

The rheological properties of bread dough made with various ratios of garden cress seed (GCS) and refined wheat flour were investigated. The Brabender Farinograph E, Extensograph E and Amylograph E were used to determine rheological properties such as water absorption, development time, extensibility, proving time, tolerance index, and gelatinization. Refined wheat flour proportion was kept constant, whereas that of GCS had prior treatment of germinated (G), grounded/untreated (U), and roasted (R) by 2%, 4%, and 6%, respectively. The values for water absorption (500 B.U.) ranged from 66.4% to 71.6%, whereas the values for water absorption (14.0%) ranged from 63.3% to 70.7%. The development times for the flour blend formulations ranged from 5.2 to 6.5 min for samples T0, T10G to T18R. The tolerance index improved from sample T0, T10G to T18R with the inclusion of GCS flour, i.e. from 73 to 113 BU. Extensograph values were taken at curves corresponding to proving periods of 30, 60, and 90 minutes. The energy area declined and the extension resistance increased as the proving time increased. All of the sample ratio values were between 1.5 and 5. The dough obtained from mixes of roasted GCS and untreated i.e. powered GCS with refined wheat flour enhanced the amylographic parameters of the amylogram peak maximum, temperature, and gel viscosity. T16R attained the highest point at 59.5ºC, followed by T15U6, T14U4, and T13U2 at 58.4, 57.1, and 55.9 ºC, respectively. The results demonstrated that combining GCS flour improved the rheological properties and quality of the flour dough. This dough can be used to manufacture bread and other bakery goods because to its high nutritional value and beneficial benefits on human health.

Meiotic analysis of induced translocation heterozygotes in Lepidium sativum Linn.

Aim: To assess the cytological effects of Gamma rays on the pollen mother cells and post meiotic products of Lepidium sativum Linn. which resulted in isolation of translocation heterozygote and aneuploid gametes. Methodology: During the experiment, parameters such as Meiotic stages, chromosomal structure, and pollen fertility were studied. Fresh seeds of Garden cress were orderly arranged in plastic and divided into four groups. The fresh seeds of each group were individually irradiated with 60Gy, 150Gy, 300Gy, 450Gy and 600Gy @ 7.247KGyh-1 in the Floriculture Laboratory of National Botanical Research Institute (NBRI), Lucknow (India). The source for gamma ray irradiation was Cobalt-60. The irradiated seeds were sown in triplicates along with their respective controls. Results: Meiotic analysis of pollen mother cells (PMCs) at 300Gy showed translocation heterozygotes, however, in the control sets, the rate was negligible and phenotypically the plants were weak and short heighted. The cytological illustration of chromosomal configuration at diakinesis and metaphase I exhibited the superiority of tetravalents, bivalent and other forms of chromosomal associations such as multivalents. The induced translocation heterozygotes resulted in stickiness at metaphase and anaphase states along with the formation of bridges at anaphase stage. Due to chromosomal anomalies, the pollen fertility was reduced to 37.54±0.25% in contrast to control plants (98.20±0.16%). Interpretation: The current research work would be of paramount interest as translocation heterozygote serves as a source for raising aneuploid offspring having novel genetic combinations. Key words: Chromosomal anomalies, Heterozygotes, Lepidium sativum Linn, Meiosis, Pollen fertility

Garden Cress Seed Oil Abrogates Testicular Oxidative Injury and NF-kB-Mediated Inflammation in Diabetic Mice.

Diabetes mellitus is a metabolic disorder associated with various complications encompassing male reproductive dysfunction. The present study aimed to investigate the therapeutic potential of biologically active Lepidium sativum seed oil (LSO) against the testicular dysfunction associated with streptozotocin (STZ)-induced diabetes. Male adults (n = 24) were divided into four groups: control, LSO-administered, diabetic (D), and LSO-treated diabetic (D+LSO) groups. LSO was extracted from L. sativum seeds, and its chemical composition was determined using GC-MS. Serum testosterone levels, testicular enzymatic antioxidants (catalase (CAT) and superoxide dismutase (SOD)), an oxidative stress (OS) biomarker, malondialdehyde (MDA), pro-inflammatory markers (NF-kB, IL-1, IL-6, and TNF-α), and the expression level of NF-kB were assessed. In addition, histopathological changes were evaluated in testicular tissues. The results obtained showed that the chemical composition of LSO indicated its enrichment mainly with γ-tocopherol (62.1%), followed by 2-methylhexacosane (8.12%), butylated hydroxytoluene (8.04%), 10-Methylnonadecane (4.81%), and δ-tocopherol (3.91%). Moreover, LSO administration in the D+LSO mice significantly increased testosterone levels and ameliorated the observed testicular oxidative damage, inflammatory response, and reduced NF-kB expression compared to the diabetic mice. Biochemical and molecular analyses confirmed the histological results. In conclusion, LSO may prevent the progression of diabetes-induced impairment in the testes through inhibition of the OS- and NF-kB-mediated inflammatory response.

Development and Quality Evaluation of Garden Cress (Lepidium Sativum L.) Seeds Supplemented Panjiri for Lactating Mothers

Lepidium sativum also referred to as garden cress, is a fast-growing annual herb that originated in West Asia and Egypt but is now grown all over the world. Its seeds are a great source of iron, omega-3 fatty acids, dietary fibre, protein, and other vital nutrients and phytochemicals. Garden cress seed- an unexplored health grain, is a rich source of nutrients, nutraceuticals, and possess numerous health benefits including its galactagogue properties. Despite having significant medicinal potential, garden cress seeds has not received the attention it deserves and has often been underutilised. Galactagogue properties of garden cress seeds promote lactation and prevent postnatal complications. Considering this property nutritious, convenience ready-to-eat panjiri (sund) was prepared for the lactating mothers. Incorporation of garden cress powder significantly and proportionately affected the sensory attributes of panjiri formulations. There was significant and proportionate increase in macronutrients of panjiri with increase in levels of supplementation. 20 per cent incorporated recipe had higher amount of ash, crude fat, crude fibre and crude protein (2.89, 37.65, 3.34 and 9.44 % respectively) as compared to control and 5, 10, 15 per cent incorporated panjiri.

Nutritional and Sensory Evaluation of Low-Cost Iron Rich Recipes Fortified with Garden Cress Seeds (Lepidium Sativum)

Abstract: Seeds are versatile ingredient, which can be used to enhance texture and add burst of micro nutrients. Among all known seeds, Garden cress seed (Gcs) is an unpopular and often referred as Forgotten Superfood as general public is unaware of its inexpensiveness and nutritive potential which is yet not utilised to its fullest. Gcs comes with hampered number of micronutrients, 100 gm of Gcs contain 723mg phosphorus, 377 mg calcium and 100 mg iron. This study focuses on the development of low-cost iron rich recipes using Gcs. The two recipes developed were Fruit Leather (FL) and Instant Sattu premix drink (SD). Three variants for each recipe were created, as FL1, FL2, FL3 and SD1, SD2, SD3; with different level of fortification, which was first sensory analysed, to estimate the most accepted variants. The best accepted variant FL3 and SD2 was not significant (p>0.05) different in other attributes. Nutrients like iron. protein, carbohydrate, and fibre were analysed. FL cover 11.25% DV of total iron requirement, SD comprehend 37.50%. Both the product contains beneficial amount of protein, carbohydrates and fibre, which will aid both adolescence and people of older age. The cost of the products was relatively lower than the market price, total production cost of FL was 12.5 Rs/100gm and SD cost for 18.5 Rs/100 gm. Both the products are worthwhile as they provide such vital nutrients in abundant on lower cost.

Characterization of free oligosaccharides from garden cress seed aqueous exudate using PGC LC-MS/MS and NMR spectroscopy

Garden cress seeds produces mucilage that has found various food applications, however, there is little information on the free oligosaccharides (FOS) contents in these seeds. Herein, we explored the presence of FOS in cress seed aqueous exudate. PGC-LC MS/MS analysis indicated the presence of mainly hexose containing oligosaccharides such as raffinose, stachyose and verbascose belonging to raffinose family of oligosaccharides (RFOs). In addition, minor fraction of planteose, isomeric tri- and tetrasaccharides were also observed. Further, the structural confirmation of the abundant tri- and tetrasaccharide were obtained through 1D and 2D NMR analysis. Thus, the RFOs presence in cress seeds would enhance its bio-functionalities.

Effects of pre-treatments on nutritional and mineral composition of garden cress seeds (Lepidium sativum)

This study aimed to recognize an effective treatment-time combination for improving nutritional value of Garden cress seeds (GCS). In this study GCS were subjected to different pre-treatments combinations viz., BoR- boiling (10 min) + roasting (2.5, 5 and 7.5 min), BlR- blanching (3 min) + roasting (2.5, 5 and 7.5 min) and SR- soaking (12 hrs) + roasting (2.5, 5 and 7.5 min), respectively. Results revealed for proximate composition showed slight variation in protein content ranged from 21.08 to 22.90% among treatments. Results for treatment SR 2.5 showed significant (p<0.05) increase in ash content from 4.62 to 5.24%. Results reported for treatment BoR showed significant improvement in iron content (10.84 mg/100 g) with increase in roasting time i.e., treatment BoR 7.5. The significant (p < 0.05) decrease in anti-nutritional factors from 458.3 to 414.5 mg/100 g for phytates, and 152.11 to 93.93 mg/100 g for oxalates were observed in treatment SR 7.5 with maximum retention of calcium of 378 mg/100 g. It could be concluded that overall improvement in nutrient content and maximum nutrient retention was observed during soaking and subsequent roasting. Thus, treatment SR 7.5 can be considered as best pre-treatment for enhanced nutritional value and decreased antinutritional profile of GCS.

Phytochemicals, phenolic compounds and antioxidant activity of garden cress (Lepidium sativum L.) seeds

Phytochemicals, phenolic compounds and antioxidant activity of garden cress (Lepidium sativum L.) seeds

Enhanced selectivity and recovery of phosphate and nitrate ions onto coffee ground waste biochars via co-precipitation of Mg/Al layered double hydroxides: A potential slow-release fertilizer

In this study, the feasibility of Mg/Al layered double hydroxides (LDH) functionalized coffee ground waste biochars (LDHMgAl@CWGB) as a potential adsorbent to selectively recover phosphate (PO43−) and nitrate (NO3−) ions in aqueous phases and their consecutive uses as a slow-release fertilizer for stimulating the plant growth were identified. The higher adsorption capacity of PO43− and NO3− ions by LDHMgAl@CWGB (PO43− = 6.98 mgP/g, NO3− = 2.82 mgN/g) compared with pristine coffee ground waste biochars (CWGB; PO43− = 0.19 mgP/g, NO3− = 0.32 mgN/g) was mainly due to the incorporation of Mg/Al mixed oxides and Cl contents. Chemisorption and intra-particle mainly controlled the adsorptive recovery of PO43− and NO3− ions by CWGB and LDHMgAl@CWGB in aqueous phases and their adsorption toward CWGB and LDHMgAl@CWGB proceeded endothermically and spontaneously. The changes in the major adsorption mechanisms of PO43− and NO3− ions from ligand exchange (CWGB) to electrostatic surface complexation and anion-exchange (LDHMgAl@CWGB) supported the conclusion that the alternation of the surface features through Mg/Al LDH functionalization might improve selectivity and adsorption capacity of PO43− and NO3− ions onto CWGB under the co-existence of Cl−, SO42−, and HCO3− ions. Since PO43-- and NO3−-loaded LDHMgAl@CWGB exhibited much higher seed germination, root and shoot growth rates of garden cress seeds (Lepidium sativum L) than other liquid and solid matrices, including 5 mgP/L PO43− and 5 mgN/L NO3−, 10 mgP/L PO43− and 10 mgN/L NO3−, and LDHMgAl@CWGB, it can be postulated that PO43-- and NO3−-loaded LDHMgAl@CWGB could be practically applicable to the agricultural field as a slow-release fertilizer to facilitate the seed germination, root and shoot growth of the plants.

Formulation and Standardization of Garden Cress Seed Incorporated Snack Products

Garden cress (Lepidium sativum, family- Brassicacea) is a herbaceous plant that grows typically upto 50-60cm. It has may medicinal uses which includes like helping with hiccups, dysentery and many more. The abstract contains the procedure for the snack products.

Hemoglobin, Iron Deficiency Anemia, Role of Halim Seeds to Eradicate the Ailment: A Complete Overview

The Lepidium Sativum is also known as garden cress seeds or the Halim seeds is a commonly known herb for its plethora of health benefits. Moreover they have been proved to be a rich source of ample of nutrients as well as phytochemicals. In addition to this the garden cress seeds are a rich source of iron which is absorbed in the small intestine effortlessly and helps in the elevation of the hemoglobin levels of the human body in the blood. Furthermore, the seeds of the garden cress have some remarkable medicinal properties such as anti-anemic, anti-diabetic effect, anti-oxidant effect etc. the Halim seeds or the garden cress seeds are composed of not only a good amount of iron but also the vitamins such as C, E, A, B12, as well as protein. Anemia is an ailment in which the number of red blood cells is insufficient which yields to the constant decrement in the oxygen carrying capacity that does not necessarily meet the physiological needs of the body. Since the ancient times the Halim seeds have been used as the traditional medicines. They are a rich source of carbohydrates, protein, lipids, crude fibers, etc. Afterwards the Vedic era the Garden cress has been considered as an important plant with not only rich nutritional values but also medicinal values. Henceforth the seeds of garden cress can be used for treating the condition of iron deficiency anemia as they have anti anemic properties which has an enormous ability to boost up the hemoglobin levels of the body.

Garden Cress (Lepidium sativum) Seeds Ameliorated Aluminum-Induced Alzheimer Disease in Rats Through Antioxidant, Anti-Inflammatory, and Antiapoptotic Effects.

Bioaccumulation of aluminum in the brain is associated with adverse neuroinflammatory and neurodegenerative changes, such as those seen in Alzheimer's disease (AD). This study aimed to assess the impact of the administration of Lepidium sativum (LS) extract on behavioral, biochemical, and cerebral histopathological changes in rats with AlCl3-induced AD and explore the mechanism behind this effect. This study was conducted on 40 male albino rats divided into four groups (n=10): LS (control, 20 mg/kg body weight for 8 weeks), AD (AlCl3, 10 mg/kg body weight), and an LS-treated AD group. Behavioral assessment included radial armed maze and active avoidance training tests. Proinflammatory cytokines, oxidant/antioxidant markers, Aβ, AchE, tau protein, TGFβ1, homocysteine, folic acid, and vitamin B12 were biochemically assessed in the serum. The cerebral cortex was histopathologically examined. AlCl3 administration significantly impaired rats' memory, indicating AD-like behavioral changes, significantly increased (P<0.001) oxidative stress markers, enhanced proinflammatory cytokines, and significantly increased AChE (P<0.001) adding to cytotoxic effects and neuronal loss in the cerebral cortex. LS administration significantly improved the antioxidant parameters, reduced proinflammatory cytokines, and alleviated AD-associated histopathological changes. LS ameliorated AlCl3-induced changes through its antioxidant, anti-inflammatory, and antiapoptotic effects, suggesting that it has a neuroprotective effect.

Phytochemicals, Phenolic Compounds and Antioxidant Activity of Garden cress (Lepidium sativum L.) seeds

Phytochemicals, Phenolic Compounds and Antioxidant Activity of Garden cress (Lepidium sativum L.) seeds

In silico molecular modeling of cold pressed garden cress (Lepidium sativum L.) seed oil toward the binding pocket of antimicrobial resistance Staphylococcus aureus DNA-gyrase complexes.

The seeds of garden cress, Lepidium sativum L., are a fantastic source of phytochemicals and proteins. The purpose of the current study was to use solvent extraction techniques to examine the physicochemical characteristics and biological activities of garden cress (L. sativum) seed oil extracts and compounds against Staphylococcus aureus, in vitro, molecular docking and pharmacokinetics. Cress seed oil were collected from Sakaka, Saudi Arabia's Al-Jouf market. Seeds were crushed in 80% ethanol for several extraction. The oil extraction was forced through a perforated tube, and the meal was expelled via a calibrated aperture. After that, a centrifuge was used to separate the oil from the plant debris (15 min). Study the anti- Staphylococcus aureus of cress seed oil by Well-Diffusion Assay, while cress oil molecules docked against Staphylococcus aureus target (pdb-id: 2XCS) by MOE 19.0901 Software. The pharmacokinetics (ADMET) and Lipinski's rules were predicted by pKCSM online server (available at: https://biosig.lab.uq.edu.au/pkcsm/prediction). The outcome showed that the oil yield for seed oil extract, the specific gravity (0.93) and concentration (33%) was substantially greater. Our findings included a maximal zone of inhibition (23 mm), a minimum inhibitory concentration (MIC) of 80 µg/mL, and a minimum bactericidal concentration (MBC) of 170 µg/mL of cress oil against Staphylococcus aureus. The docking results indicated that the affinity score of Quercetin-3-O-glucosylgalactoside docked against pdb-id: 2XCS was 9.48, while RMSD 1.59 Å compared with the co-crystallized ligand showed an affinity score of -7.58 kcal/mol and RMSD 1.32 Å. Our findings suggest that Cress seed oil might be utilised to protect food from S. aureus infection that is resistant to antibiotics.