The use of unconventional food plants is an alternative for diversification and promotion of food sovereignty. The objective of this work was to evaluate the quality of spinach seedlings from the Amazon produced in samaúma substrate as conditioner. The experiment was carried out in a greenhouse in the experimental garden of the Federal University of Acre, from November to December 2022. The experimental design was completely randomized, with three treatments and 30 repetitions, totaling 90 experimental units. The treatments were 3 substrates, as follows: T1 - Commercial substrate; T2 -commercial substrate + samaúma substrate (1:1) and T3 – samaúma substrate. At 30 days of cultivation, the following variables were evaluated: leaf length, leaf width, seedling height, stem diameter, total number of shoots, total number of leaves, number of leaves per shoot, root length, fresh mass of the part aerial part, fresh mass of roots, dry mass of aerial part, dry mass of roots, total fresh mass, total dry mass and the Dickson quality index was calculated. The combination of the commercial substrate with the samaúma substrate (1:1) is an alternative for the production of spinach seedlings from the Amazon.