Rice seedlings of different stages of development were infested with tadpole shrimp (TPS, Triops longicaudatus [LeConte]), of different sizes in four experiments conducted in a lath house in Colusa, CA, during 2015 and 2016. Tadpole shrimp size was based on carapace length. Rice seedling stages were classified as S0 if no structures were present; S1 if only the coleoptile was present; S2 if the coleoptile and radicle were present; S3-I if the prophyll had emerged; S3-II if the first true leave had emerged but was not unfurled; and V1 if the first true leave was unfurled. Results showed that TPS of all sizes are capable of injuring rice and reducing seedling establishment, with larger TPS causing more seedling establishment reduction than medium or small TPS. The coleoptile, radicle, and roots were the structures most injured by TPS. During the S0 stage, TPS buried seeds, preventing their establishment. Seedling stages S1, S2, and S3-I were found to have the highest risk of injury by TPS, while seedling stage V1 had the lowest. Pearson's correlation between injured seedlings and the normalized number of established seedlings indicates that injury to the coleoptile, root, and radicle combined are the most important in preventing seedling establishment.