Seed Size Research Articles

Chapter 16: Rules for size and grading of seeds

Chapter 16: Rules for size and grading of seeds

Legume Seed Sizes and Their Consequential Growth Performance

Legume Seed Sizes and Their Consequential Growth Performance

Diversity of Seed Size and Flesh Rate of Litchi ‘No Mai Tsz’ Fruits Available in the Taiwanese Market

Diversity of Seed Size and Flesh Rate of Litchi ‘No Mai Tsz’ Fruits Available in the Taiwanese Market

Genetic Parameters for Determining Useful Parents in Mungbean (Vigna radiata (L.) Wilczek) Breeding for Early Maturity, Small Seed Size, and High Seed Yield.

Early maturity, small seed size, and high seed yield are important characters of mungbean in Indonesia. The objective of the study was to determine the useful parents in mungbean crosses for early maturity, small seed size, and high seed yield varieties by estimating the genetic parameters and their inheritance. The study was conducted at the ILETRI, Malang, East Java, Indonesia, during the dry season of 2014. 20 F1 and 5 parents were evaluated using a randomized block design, repeated three times. Results of the study showed that all observed traits showed the importance of both additive and dominance gene effects. The relative value of general combining ability (GCA) was greater than specific combining ability (SCA) for number of pod clusters per plant, number of branches per plant, plant height, days to maturity, and 100-seed weight which indicated the importance of additive gene effect. The dominance gene effect occurred on number of pods and seed yield per plant. Among five parents, G3 was the best combiner for all the observed characters except pod length; therefore, G3 could be exploited for late maturity, small seed size, high number of branches and pod cluster, and high seed yield. G5 has a high GCA for 100-seed weight. G1 and G2 have good GCA for early maturity. G3 and G5 genotypes are useful as parents in mungbean breeding for small and large seed size varieties, respectively. The best combination for seed yield was G2 × G3 and G3 × G1 crosses and could be proceeded with selection for early maturity, small seed size, and high seed yield varieties.

OsFH3 Encodes a Type II Formin Required for Rice Morphogenesis.

The actin cytoskeleton is crucial for plant morphogenesis, and organization of actin filaments (AF) is dynamically regulated by actin-binding proteins. However, the roles of actin-binding proteins, particularly type II formins, in this process remain poorly understood in plants. Here, we report that a type II formin in rice, Oryza sativa formin homolog 3 (OsFH3), acts as a major player to modulate AF dynamics and contributes to rice morphogenesis. osfh3 mutants were semi-dwarf with reduced size of seeds and unchanged responses to light or gravity compared with mutants of osfh5, another type II formin in rice. osfh3 osfh5 mutants were dwarf with more severe developmental defectiveness. Recombinant OsFH3 could nucleate actin, promote AF bundling, and cap the barbed end of AF to prevent elongation and depolymerization, but in the absence of profilin, OsFH3 could inhibit AF elongation. Different from other reported type II formins, OsFH3 could bind, but not bundle, microtubules directly. Furthermore, its N-terminal phosphatase and tensin homolog domain played a key role in modulating OsFH3 localization at intersections of AF and punctate structures of microtubules, which differed from other reported plant formins. Our results, thus, provide insights into the biological function of type II formins in modulating plant morphology by acting on AF dynamics.

Investigation of Operating Parameters on Ultrasound-Assisted Extraction of Anethole in Fennel Essential Oil

In this paper, the operational impact of three parameters including power of ultrasonic apparatus, size of fennel seeds and experiment time on the extraction yield of Anethole, which is the main considerable component in fennel essential oil and its concentration have been studied through Ultrasound-Assisted Extraction. The ultrasonic extraction of oil from fennel seeds using a solution of 70% water-ethanol was studied at different particle sizes, different ultrasonic powers and three different levels of time. The most effective parameter was particle size, while the experiment time had the least impact on both the efficiency and Anethole concentration as well. As a result, compared to Soxhlet method, the ultrasonic-assisted extraction was more efficient. In this experiment, eighteen constituents were identified for fennel seeds using GC–MS. The major components were Anethole (78.12%), Fenchone (8.81%), Limonene (4.39%), and Estragole (4.52%). Furthermore, the analysis of two quadratic models using the Box-Behnken design (BBD) indicated that the quadratic polynomial model can be applied for estimating the Anethole extraction yield as well as Anethole concentration.

Dual and opposing roles of EIN3 reveal a generation conflict during seed growth

Seed size critically affects grain yield of crops and hence represents a key breeding target. The development of embryo-nourishing endosperm is a key driver of seed expansion. We here report unexpected dual roles of the transcription factor EIN3 in regulating seed size. These EIN3 functions have remained largely undiscovered because they oppose each other. Capitalizing on the analysis of multiple ethylene biosynthesis mutants, we demonstrate that EIN3 represses endosperm and seed development in a pathway regulated by ethylene. We, in addition, provide evidence that EIN3-mediated synergid nucleus disintegration promotes endosperm expansion. Interestingly, synergid nucleus disintegration is not affected in various ethylene biosynthesis mutants, suggesting that this promoting function of EIN3 is independent of ethylene. Whereas the growth-inhibitory ethylene-dependent EIN3 action appears to be encoded by sporophytic tissue, the growth-promoting role of EIN3 is induced by fertilization, revealing a generation conflict that converges toward the key signaling component EIN3.

Abundance and Habitat Preference of the Genus Euplectes in Protected and Non protected Habitats of Jos East Plateau State, Nigeria

The knowledge on the factors influencing habitat selection is of uttermost importance because it guides the development of conservation action plan for species. Till date, there is no baseline study on the genus Euplectes in Northern Nigeria thus, an attempt to investigate the habitat preference of the genus was carried out in Amurum Forest Reserve and the nonprotected habitats of Fobur. Line transects of 200m were randomly laid across the study sites. The abundance of Bishop species was highest in farmland compared to grassland and rocky outcrop. Therefore, the number of the species was significantly different between habitats (F2, 573 = 5.454, P = 0.0015). There was a significant relationship between flock size and number of seeds across habitat types. Number of individual birds increased with increase in seed number (F1, 57 = 21687.786, P < 0.001). With the exception of grass height, there was a highly significant difference in relation to vegetation variables taken between plots where Bishop species were sighted and the random points where they were not seen (P < 0.001). The finding suggests that farmlands should be integrated into conservation plan in the nonprotected area based on the high population of the genus recorded.

Correction to: Maternal environment and seed size are important for successful germination and seedling establishment of Pterocarpus erinaceus (Fabaceae)

Seed size and the growth environment are important variables that influence seed germination, growth and biomass of seedlings and future tree harvest and should thus be taken into account in agroforestry and reforestation programmes for endangered species like Pterocarpus erinaceus. In the present study, to assess seedling germination and vigour in P. erinaceus as a function of seed size in two environments, 1080 seeds and 360 seedlings were evaluated at two separate sites in Cote d'Ivoire. The results show that large seeds had very high germination rates (up to 100%) and produced more vigorous plants better able to adapt to climate change. The maternal environment and seed size had a significant influence on seed germination (P < 0.05) and seedling development (P < 0.05) and biomass (P < 0.05). Seedlings were most successful at the site with a humid tropical climate (Daloa). Seedling leaves had the same resistance regardless of seed size and study site, but leaf moisture content was more stable in seedlings grown from medium and small seeds. These results will help guide conservation strategies for the species and are key factors for rural populations, loggers, and forest management structures for the silviculture of this species.

Effect of Seed Size and Different Pretreatment Methods on Germination of Albizia zygia (DC.) J. F. Macbr

Albizia zygia produces valuable timber which has received some level of prominence in the international market. However, the seeds are dormant, and the tree species remain undomesticated. This study was conducted to evaluate the effect of seed size and presowing on the germination of Albizia zygia in a nursery. Seeds were grouped into four categories in regard to their length, small (≤0.5 cm), medium (&gt; 0.5 &lt; 0.8 cm), large (≥0.8 cm), and mixture of small, medium, and large seeds. The seeds were subjected to five main seed pretreatment methods, namely, soaking in sulphuric acid (H2SO4) for 2 minutes, mechanical scarification, soaking in hot water for 5 minutes, soaking in cold water for 24 hours, and control where seeds were sown without any treatment. The results indicate that combination of mechanical scarification and large seeds produced the highest (100%) germination. Hot water treatment was effective in large seeds producing 69.0% germination. The increased germination for mechanically scarified seeds suggests that seed dormancy in Albizia zygia is mainly due to its hard seed coat. Therefore, it is recommended to farmers to adopt use of mechanical scarification and large seeds, since it is safe and effective.

A Low-Power GPIO-Based Size Sensor to Monitor the Imbibition of Corn Seeds Beneath Soil

Seed imbibition, absorption of water by a dry seed, is an essential process in which embryo hydration and root establishment occur. In natural environments, this process occurs beneath the soil, making it difficult to observe preliminary growth of seeds. This paper presents a new technique for tracking the imbibition of corn seeds. The proposed system is designed to measure imbibition through seed expansion and wirelessly transmit data, permitting the system to remain beneath the soil with the subject seed. The system consists of low-cost commercial off-the-shelf components and 3D-printed probes. The proposed system is optimized to measure the size of multiple seeds with a single Analog-to-Digital Converter (ADC) pin by utilizing the General-Purpose Input Output (GPIO) pins of the microcontroller, to reconfigure connections to supply voltage or ground. The circuit design of the system shows low power consumption compared to other conventional circuits and utilizes fewer components by taking advantage of the microcontroller GPIOs. Additionally, the proposed circuit design shows less error and insensitivity to the supply voltage variations.

Phenotypic (Fruit and Seed Characters) Selection of Dacryodes edulis (Don. G. Lam H. J.) Tree for Vegetative Propagation

The study was conducted to identify superior D. edulis trees using phenotypic characters (fruit and seed size) as the criteria to select candidate trees for subsequent multiplication through vegetative propagation. Five D. edulis compound trees were randomly selected within Onne community in Eleme LGA Rivers state in a preliminary effort to assist households in the selection and multiplication of desirable tree phenotypes. Seeds were extracted by softening fruit with warm water at 57oC. Fruit and seed length (mm), breadth (mm), and thickness (mm) were taken using veneer caliper. Size of fruit and seed was calculated as: length × breadth × thickness. The experiment was a completely randomized design in its layout and data analysis was carried out using analysis of variance and regression after a normality test was conducted using Shapiro-Wilk. The results showed that highest mean fruit size (79.38 ± 3.99 mm) was recorded in tree 3 and the lowest was tree 5 (29.60 ± 1.48 mm): while, highest seed size (34.78 ± 2.47 mm) was recorded in tree 3 and the lowest in tree 4 (15.58 ± 0.99 mm). Highest within tree fruit size variability was recorded in tree 1 (24%) and the lowest in tree 4 (12%): while the highest within tree seed size variability was recorded in tree 2 (28%) and the lowest in tree 5 (15%). There was however a significant difference in fruit and seed size between the trees. Pairwise comparison showed that tree 1 was not significantly different from tree 2 in fruit and seed size. There was a significant positive correlation between fruit and seed size among the trees. This implies that selection for large fruit size automatically selects for large seeds size. Large fruited trees can therefore be multiplied from these trees using vegetative propagation.

UJI EFISIENSI PENAMBAHAN FEED SUPPLEMENT DENGAN DOSIS BERBEDA TERHADAP RETENSI PROTEIN DAN KELANGSUNGAN HIDUP UDANG VANNAMEI (Litopenaeus Vannamei)

The purpose of this study is; to determine the effect of feed supplement on different doses of feed can improve the survival and retention of vannamei shrimp protein. The method used is the Design Randomized (RAK), consisted of 4 treatments, 3 times repetition and 1 control. Every place of cultivation has spread vannamei shrimp seeds size 5 – 7 cm with the density of 20 tails / m2. Applying fish oil of every treatment of shrimp feed with appropriate concentration related to treatment, those are: F0 = Control (0 ml/kg), F1 = 5 ml/kg, F2 = 10 ml/kg dan F3 = 15 ml/kg. Based on the result of the research study, able to conclude that giving a dose of feed supplement to vannamei shrimp shows that real difference in the survival rate variables. The treatment of F2&#x0D; (10 ml/kg feed) is the best treatment. While the variable protein retention showed no significant difference in&#x0D; all treatments.

Role of lurkers in threshold-driven information spreading dynamics.

The threshold model as a classical paradigm for studying information spreading processes has been well studied. The main focuses are on how the underlying social network structure or the size of initial seeds can affect the cascading dynamics. However, the influence of node characteristics has been largely ignored. Here, inspired by empirical observations, we extend the threshold model by taking into account lurking nodes, who rarely interact with their neighbors. In particular, we consider two different scenarios: (i) Lurkers are absolutely silent and never interact with others and (ii) lurkers intermittently interact with their neighborhood with an activity rate p. In the first case, we demonstrate that lurkers may reduce the effective average degree of the underlying network, playing a dual role in spreading dynamics. In the latter case, we find that the stochastic dynamic behavior of lurkers could significantly promote the spread of information. Concretely, slightly raising the activity rate p of lurkers may result in a remarkable increase in the final cascade size. Further increasing p could make nodes become more stable on average, while it is still easy to observe global cascades due to the fluctuations of the effective degree of nodes.

Sound Splits as Influenced by Seed Size for Runner and Virginia Market Type Peanut Shelled on a Reciprocating Sheller

The objective of this study was to examine peanut (Arachis hypogaea L.) kernel percent sound splits as a function of sound mature kernel seed size when shelled on a reciprocating sheller. Data were compiled from a total of 139 field experiments conducted in the Virginia-Carolina region and Georgia from 2005 to 2020. Runner and Virginia peanut market types were graded according to United States Department of Agriculture (USDA) standards using standard sheller screens with upper grid sizes corresponding to the red pan from the pre-sizer of 10.3 × 19.1 mm (26/64 × 3/4 ″) and 13.5 × 25.4 mm (34/64 × 1 ″) with minimum bar grid clearances of 8.7 (11/32 ″) and 12.7 mm (1/2 ″), respectively. A subset of runner market type samples was graded using the Virginia sheller screen. Grade data per market type and sheller screen was analyzed separately. Among runner market types shelled with the standard runner-type screen, percent sound splits increased linearly with increasing seed size at the logit rate of 1.16 per sound mature kernel g (p &lt; 0.001). Sound splits for Virginia and runner market types shelled on the standard Virginia-type screen did not significantly vary by kernel size (p = 0.939 and 0.687, respectively). Extra-large kernels (proportion) for Virginia types linearly increased with seed size at 1.91 per sound mature kernel g (logit scale) (p &lt; 0.001). Runner market types sized 75 to 91 g/100 sound mature kernels (605 to 500 seed/lb) were estimated to have a 50% probability of a 2.3 to 4.5% or greater increase in sound splits when shelled with the standard runner-type screen compared to runner-type seed sized 55 g/100 sound mature kernels (820 seed/lb), respectively, equivalent to a potential deduction increase of 1.8 to 4.4 USD /1000 kg. For both Virginia and runner market types, seed weight linearly increased with pod weight at 0.169 and 0.195 g/g (p &lt; 0.001), respectively. Results from this study may be used as a reference to suggest runner-type seed sizes above which larger reciprocating sheller screen utilization in line with USDA grading practices is warranted to reduce mechanically induced sound splits during grading and subsequent economic deduction penalties for corresponding farmer stock peanut.

Effect of Seed Size and Salinity on Germination and Vegetative Growth of Wheat (Triticum aestivum L.) Variety Imam

To investigate the effect of seed size and salinity stress on the germination and vegetative growth of wheat variety Imam, a laboratory and a pot experiment were conducted during the winter season of 2016. The experiments were conducted at the Seed Laboratory of Agronomy Department and at the Nursery, Faculty of Agriculture, University of Khartoum, (Shambat). Seed size treatments were selected on the basis of seed diameter, and were categorized into three classes of small; medium and large.Salinity concentrations were: EC0 (control); EC2 (0.24 mM NaCl L-l); EC4 (0.28 mM NaCl L-l); EC6 (0.39 mM NaCl L-l); EC8 (0.41 mM NaCl L-l) and EC10 (0.59 mM NaCl L-l). The experiments were conducted using a completely randomized design with two replicates for the germination test and three replicates for the pot experiment. Data were collected on:germination percentage on the 4th and 8th day after planting; plant height; number of leaves per plant; leaf area; number of tillers per plant and dry matter yield. The data were statistically analyzed using ANOVA and mean separation using LSD. The results revealed that seed size class did not show significant (p ≤ 0.05) effect on germination percent of wheat. However, salinity significantly (p ≤0.05) affected germination at both readings. Small seed class registered higher germination at EC4 and EC10. For the pot experiment, the results revealed that seed size class did not have a significant (p ≤ 0.05) effect on the studied parameters. However, better performance was observed for the large seed size class as compared to the other seed size classes. Salinity had a significant (p ≤ 0.05) adverse effect on all measured growth parameters and reduced dry matter yield by 10.4 %, 11.7 %, 47.7% , 38.5 % and 72.2 % at EC2 and EC4, EC6, EC8 and EC10, respectively, as compared to EC0. Except for leaf number, most of the interactions between seed size and salinity were significant (p ≤ 0.05). Further research is neededto confirm the reported effect of salinity on growth and yield of wheat variety Imam.

The upsizing of the São Tomé seed dispersal network by introduced animals

Biological invasions are a major threat to global biodiversity with particularly deleterious consequences on oceanic islands. The introduction of large terrestrial animals – generally absent on islands – can disrupt important ecosystem functions, such as the dispersal of native seeds. However, while the consequences of plant invasions received much attention, the potential of introduced animals to change insular seed dispersal networks remains largely unknown. Here, we collated evidence from five sampling methods to assemble qualitative and quantitative, multi‐guild seed dispersal network for the island of São Tomé (Gulf of Guinea) and explore whether native and introduced seed dispersers consistently differ in their topological roles, in their gape width and in the size of the dispersed seeds. Our network included 428 interactions between 23 dispersers (14 birds, 2 bats, 1 snake and 6 non‐flying mammals) and 133 plant species. Each method (direct observations, identification of seeds in droppings and stomachs, questionnaires and literature review) was particularly informative for a small group of dispersers, thus rendering largely complementary information. Native and introduced dispersers did not differ in their topological position in either qualitative or quantitative networks (linkage level, specialization d' and species strength). However, introduced dispersers tend to have much larger gape widths and to disperse significantly larger seeds. Our results point to a general upsizing of the seed dispersal network in the island of São Tomé driven by the recent arrival of large, introduced animals. We argue that this pattern is likely common on other oceanic islands where introduced dispersers might counteract the general pattern of seed dispersal downsizing resulting from the selective extinction of larger animals.

Double Mutant Analysis with the Large Flower Mutant, ohbana1, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in Arabidopsis thaliana.

Two growth processes, cell proliferation and expansion, determine plant species-specific organ sizes. A large flower mutant in Arabidopsis thaliana, ohbana1 (ohb1), was isolated from a mutant library. In the ohb1 flowers, post-mitotic cell expansion and endoreduplication of nuclear DNA were promoted. The whole-genome resequencing and genetic analysis results showed that the loss of function in MEDIATOR16 (MED16), a mediator complex subunit, was responsible for the large flower phenotypes exhibited by ohb1. A phenotypic analysis of the mutant alleles in MED16 and the double mutants created by crossing ohb1 with representative large flower mutants revealed that MED16 and MED25 share part of the negative petal size regulatory pathways. Furthermore, the double mutant analyses suggested that there were genetically independent pathways leading to cell size restrictions in the floral organs which were not related to the MED complex. Several double mutants also formed larger and heavier seeds than the wild type and single mutant plants, which indicated that MED16 was involved in seed size regulation. This study has revealed part of the size-regulatory network in flowers and seeds through analysis of the ohb1 mutant, and that the size-regulation pathways are partially different between floral organs and seeds.

Morphological diversities and associated preference traits in Peanut (Arachis hypogaea L.) landraces from central and southern Benin

Peanut (Arachis hypogaea L.) production in Benin is limited by several biotic and abiotic stresses. The objectives of this study were to document varietal trait preferences and peanut genetic diversity for breeding programs. A survey in the South and Center of Benin and an agro-morphological characterization was carried out. Results showed that farmers used five criteria, namely yield (seeds and pods), size of seeds, number of seeds per pod, oil content, and quality of the paste for selecting a variety. The clustering based on ten qualitative traits showed a variability structure in three groups based on the leaflets shape, plant habit, and branching pattern. The principal component analysis carried out on the quantitative traits indicated six groups of accessions. The group 3 of the latest analysis is composed of Azii alorga and Kamalo with interesting characteristics and showing a good criteria as seeds size (SDL= 14.62 ± 0.48 mm), and pods size (average pod length and width respectively PoL = 33.70 ± 1.14 mm, Pow = 14.87 ± 0.29 mm). The results should be prioritized for breeding programs to improve the good criteria found in other peanut varieties. It will be helpful for farmers in selecting performant varieties.

The Effect of Aeroponic Potato Seeds from the Tropical Lowland and Highland Based on Size on the Growth and Yield to Become Advanced Seeds by Irrigation

Background: Potato seed production with aeroponic technology can produce a large number of seeds, besides that it is also healthy because it uses plant seeds from tissue culture. However, how the quality of aeroponic seeds produced to become the next seed (growth capacity) has not been studied and scientifically informed. This research aimed to obtain the effect of aeroponic seed yields from the low and high plains based on their size on the growth and yield into advanced seeds. Potato seeds used were from the highland and lowland using aeroponic technology. Methods: This research was conducted in the highland (1000 m above sea level) from April to July 2017. Seeds were classified as S ≤ 1 mg, 1 ≤ Md ≤ 10 g and L ≥ 10 g. This research used a randomized block design with seven repetitions. The tried factors were as follows: 1. The origin of aeroponic seeds (A): A1 (lowland), A2 (highland); 2. The size of aeroponic seeds (U): U1 (S), U2 (M), U3 (L). Plant growth parameters included plant height, number of leaves and number of tubers. Result: The data obtained were analyzed using Duncan’s Multiple Range Test (DMRT) at the 5% level. Seeds of aeroponic yields of various sizes that are planted using drip irrigation have the potential to become advanced seeds. The L-size aeroponic seeds from the lowland produced an average of 5.9 tubers with an average weight of 68.4 g.