Sediment Temperature Research Articles

Seasonal Variations in Physico- chemical Parameters of the Sediment of Thengapatnam Estuary, South-coastal Region of India

The tremendous biodiversity and ecological processes that depend on freshwater and marine environments are essential to human survival. The physicochemical parameters in both surface waters and sediments are important to understand estuarine dynamics. The present study was carried out to determine how the seasonal variation will affect the physicochemical parameters of sediments of five stations in Thengapatnam estuary. Sediment samples were collected for a period of one year from June 2014-May 2015 from five different sampling stations. The parameters such as pH, temperature, Nitrogen, Phosphorous, Potassium, Copper, Zinc, Iron and Manganese were analysed using standard procedures. The result expose that, soil sediment have high amount of potassium in all five stations. Sediment temperature varied from 25.7- 30.1°C. Hydrogen ion concentration ranged between 6.9 to 8.3, concentrations of nutrients viz. nitrate (2.4 – 6.4 mg/100g), phosphate (1.5 - 15.25 mg/100g) potassium (138 – 560 ppm) and concentration of metals like iron, zinc and manganese were found to be higher during monsoon season and lower during premonsoon seasons The overall findings highlight the need for a scientific solution to reduce the use of harmful chemicals and heavy metal contamination from the river system in order to preserve the biodiversity of freshwater and marine ecosystems.

Environmental co-limitation on temperature responses of greenhouse gas production in floodplain sediments

Despite the rising interest in understanding how climate change could affect the emissions of greenhouse gases (GHGs) from river systems, including floodplains, we still lack a mechanistic understanding of how changing environmental conditions, such as moisture and nutrient availability, limit the temperature responses of GHG production in floodplain sediments. To examine the environmental co-limitations on the temperature responses of three major GHGs (CO2, CH4, and N2O) produced in floodplain sediments, sediments from a constructed wetland on the floodplain of the lower Han River were incubated for 24 d at four temperatures spanning 4–28 ℃, under three conditions (closed, open/wetting, and open/drying). The net production of all three GHGs exhibited nonlinear temperature responses with gas-specific patterns and magnitudes of response varying over the incubation period. During the later incubation phase, positive temperature responses were weakened for the net production of CO2 and CH4 in the dried treatments, whereas a similar weakening occurred for N2O production in the wet treatments. This, combined with incubation-induced changes in dissolved organic carbon and its fluorescence components, indicated the lack or excess of moisture and associated changes in O2 and organic carbon availability as critical co-limiting factors for the temperature responses of GHG production. Warming decreased δ13C in the CH4 emitted from wet and hypoxic sediments, implying a stronger warming effect on CH4 production over oxidation. Unlike many studies assuming a consistent relationship between temperature and GHG production in sediments irrespective of other environmental conditions, our results suggest that warming effects on the GHG emissions from floodplain sediments would depend on the balance between gas production and consumption under the prevailing constraints of moisture, O2, and labile carbon availability.

Role and environmental regulation of iron-driven anaerobic methane oxidation in riverine sediment

Iron is an abundant element in the environment and acts as a thermodynamically favorable electron acceptor driving the anaerobic oxidation of methane (AOM). Presently, the role and environmental regulation of iron-driven AOM in rivers, an important source of methane emission, are nearly unknown. Here, we provided direct evidence for iron-driven AOM activity in sediment of a mountainous river (Wuxijiang River, China) through 13C-labeled isotopic experiment. The potential rate of iron-driven AOM ranged between 0.40 and 1.84 nmol 13CO2 g (sediment) d−1, which contributed 36% on average to total AOM activity when combined the potential nitrate- and nitrite-driven AOM rates measured previously. There were significant variations in iron-driven AOM rates among different reaches (upper, middle, and lower) and between seasons (summer and winter). Sediment temperature, pH, and nitrate content were closely associated with the dynamic of AOM activity. Our results indicate that iron-driven AOM has great potential for reducing methane emissions from riverine ecosystems, and suggest the necessity of taking both spatial and temporal scales into account to evaluate the quantitative role of this AOM process.

Brief communication: Testing a portable Bullard-type temperature lance confirms highly spatially heterogeneous sediment temperatures under shallow bodies of water in the Arctic

Abstract. The thermal regime in the sediment column below shallow bodies of water in Arctic permafrost controls benthic habitats and permafrost stability. We present a robust, portable device that measures detailed temperature–depth profiles of the near-surface sediments in less than 1 h. Test campaigns in the Canadian Arctic and on Svalbard have demonstrated its utility in a range of environments during winter and summer. Measured temperatures were spatially heterogeneous, even within single bodies of water. We observed the broadest temperature range in water less than 1 m deep, a zone that is not captured by single measurements in deeper water.

Differences in desiccation tolerance of two Australian freshwater mussel species with different life history characteristics is temperature dependent

Mass die-offs, reduced species richness and local extinctions of freshwater mussels have resulted from river drying events, which often co-occur with high ambient temperatures. These events are predicted to increase in frequency and severity under the influence of climate change. We aimed to identify the desiccation tolerance of two freshwater mussel species (the river mussel Alathyria jacksoni and the floodplain mussel Velesunio ambiguus) across a range of temperatures by simulating river drying events in laboratory conditions. Freshwater mussels were buried in sediment heated to 29, 32, 35, 38 and 41°C. Lethal times and lethal temperatures at which 50% mortality occurred were used to infer species-specific tolerances. The lethal time for 50% of mussels to reach mortality at 29°C was shorter for A. jacksoni (14 days) than V. ambiguus (58 days) but did not differ markedly at higher temperatures. Lethal temperatures were also similar between species over short durations (e.g. 39–40°C at 1 day). Our results suggest that the difference in desiccation tolerance between species diminishes toward their upper thermal limit. Management interventions aimed at reducing sediment temperatures, such as providing shade via riparian vegetation and wetting from environmental flows, could help alleviate the impact of drying events and climate change on both freshwater mussel species.

Study on the Influencing Factors of Injection Blockage during CO2 Sequestration in One-Dimensional Long Reactor

Carbon sequestration through CO2 injection into a formation is an effective strategy for reducing greenhouse gas emissions. In this study, a one-dimensional long reactor was constructed to simulate the CO2 injection process under various sediment temperatures, pressures, and flow rates. The formation of CO2 hydrate and the resulting blockages were investigated in detail through a series of indoor experiments. Due to the increasing driving force for CO2 hydrate formation, reducing sediment temperature and increasing sediment pressure can cause hydrate blockage to form near the injection end, leading to an increase in CO2 injection pressure and a reduction in the storage range. Furthermore, CO2 injection rate has a substantial impact on the pattern of hydrate blockage. A lower injection rate facilitates full contact between CO2 gas and pore water, which helps to increase the formation and blockage degree of CO2 hydrates, thereby decreasing the amount of CO2 injection. The experimental investigation presented in this paper examines the laws of CO2 injection and clogging under various sediment conditions and injection processes on a one-dimensional scale, which can provide valuable insights for the design of CO2 sequestration processes.

Priority sites for coral aquaculture in Kume Island based on numerical simulation

Global and local stresses such as increased water temperature and terrestrial sediment runoff significantly affect coral reef ecosystems. Over the past three decades, the increase in the intensity and frequency of coral bleaching has been a significant problem in tropical and subtropical coastal seas, and maintaining healthy coral reef ecosystems has long been a challenge. Coral cover in Shimajiri Bay on Kume Island, located in the Nansei Islands (Okinawa Prefecture, southern Japan), has declined due to exposure to high water temperatures, an outbreak of coral predators, and sediment (red soil) inflow from the land. From such a background, coral aquaculture has been implemented at a few stations in the bay. Therefore, it is essential to conduct aquaculture in suitable locations for coral growth and survival. This study aimed to select suitable locations for coral culture with high spatial resolution in an enclosed bay that was greatly affected by red soil runoff, mainly from a sugarcane field. A freshwater and red soil runoff model for the watershed was merged with a coastal hydrodynamic, red soil dispersal, and accumulation model at a spatial resolution of 75 m for Shimajiri Bay. The simulation results were validated using in situ continuous and routine observations. Simulations were conducted over ten years. Locations not subjected to high water temperature in the bay or river loading were selected based on topography, simulated water temperature, salinity, and red soil content of sea sediment. This coupled model enables us to evaluate the growth and survival conditions of cultured corals with high spatiotemporal resolution and quantitatively consider the reduction target of red soil runoff to realize robust coral growth and survival.

Temperature, Water Depth, and Flow Velocity Are Important Drivers of Methane Ebullition in a Temperate Lowland Stream

AbstractStreams and rivers are a well‐recognized source of methane (CH4), with high spatiotemporal variability in fluxes. However, CH4 release in form of bubbles (ebullition) is rarely included in current global CH4 emission estimates from lotic ecosystems, due to the lack of reliable models to upscale ebullition. Our study aimed to determine the importance of individual emission pathways (diffusion and ebullition) for total CH4 emissions from a lowland stream with low sediment heterogeneity and explore the relations of ebullition to environmental variables to build a stream ebullition model for this simplified system. We measured CH4 and carbon dioxide (CO2) diffusive emissions and ebullition from a temperate lowland stream in Czech Republic (Central Europe) during the ice‐free season 2021. The studied stream was a significant source of CH4 (mean 260 ± 107 mg CH4 m−2 day−1), with ebullition as a prevailing pathway of CH4 emission (mean 74 ± 7%, range 55%–85%) throughout the whole monitored period. CH4 ebullition showed a high spatiotemporal heterogeneity, with sediment temperature and water depth as the strongest predictors, followed by the interaction between flow velocity and sediment temperature. Our model explained 81% of total variance of CH4 ebullition and suggests that it is possible to model ebullitive fluxes in lowland streams with homogeneous sediments. Since CH4 was an important part of the total CO2‐equivalent emissions from the examined stream, accounting for mean (±SD) 35 ± 7.4%, and ebullition the majority of the CH4 emission, the ability to adequately model ebullition is pertinent for lowland streams.

Recovery of fishery-stressed seagrass meadows is driven by improvements in wastewater management

As ecosystem engineers, seagrasses provide major benefits to coastal habitats worldwide. Nonetheless, their dynamics remain poorly understood in local areas. Three seagrass (Zostera noltei) meadows located within shellfish beds in Galicia (NW Spain) were selected to assess the intra and inter-annual changes in the habitats by considering the effects of harvesting, temperature and sewage pollution. Seagrass cover increased between April and October, when the sediment temperature ranged from 15 to 20 °C, while in terms of long-term dynamics there was a mean gain of 3 % year−1 between 2004 and 2023. Shellfishing caused mechanical disturbance within the meadows, but the seasonal changes in cover depended on temperature. However, we pointed out the built of new wastewater treatment plants since 1990 that improved water quality as the main driver of the long-term increase in seagrass cover. Environmental policies that enhance seawater quality and limit harvesting pressure are essential to maintain this positive trend.

Nighttime warming and nitrogen addition effects on the microclimate of a freshwater wetland dominated by Phragmites australis

The critical impacts of microclimate on carbon (C) cycling have been widely reported. However, the potential effects of global change on wetland microclimate remain unclear, primarily because of the absence of field manipulative experiment in inundated wetland. This study was designed to examine the effects of nighttime warming and nitrogen (N) addition on air, water, and sediment temperature and also reveal the controlling factors in a Phragmites australis dominated freshwater wetland on the North China Plain. Nighttime warming increased daily air, water, and sediment temperature by 0.24 °C, 0.27 °C, and 0.36 °C, respectively. The diurnal temperature range of water was decreased by 0.44 °C under nighttime warming, whereas warming had no effect on diurnal temperature range of air and sediment. In addition, N addition caused a reduction of 0.20 °C and 0.14 °C in daily water and sediment temperature by increasing vegetation coverage. There was a significant interaction between nighttime warming and N addition on water temperature. Furthermore, the vapor pressure deficit is the main factor affecting the extent of the warming-induced increases in air temperature. The changes of height and leaf area index of Phragmites australis are responsible for the cooling effects in the N addition plots. This study provides empirical evidence for the positive climate warming – microclimate feedback in freshwater wetland. However, N deposition leads to decreased water and sediment temperature. Our findings highlight the importance of incorporating the differential impacts of nighttime warming and N addition on air, water, and sediment temperature into the predictions of wetland C cycling responses to climate change.

Intrinsic correlation between the generalized phase equilibrium condition and mechanical behaviors in hydrate-bearing sediments

The phase equilibrium and mechanical behaviors of natural gas hydrate-bearing sediment are essential for gas recovery from hydrate reservoirs. In heating closed systems, the temperature-pressure path of hydrate-bearing sediment deviates from that of pure bulk hydrate, reflecting the porous media effect in phase equilibrium. A generalized phase equilibrium equation was established for hydrate-bearing sediments, which indicates that both capillary and osmotic pressures cause the phase equilibrium curve to shift leftward on the temperature-pressure plane. In contrast to bulk hydrate, hydrate-bearing sediment always contains a certain amount of unhydrated water, which keeps phase equilibrium with the hydrate within the hydrate stability field. With changes in temperature and pressure, a portion of pore hydrate and unhydrated water may transform into each other, affecting the shear strength of hydrate-bearing sediment. A shear strength model is proposed to consider not only hydrate saturation but also the change in temperature and pressure of hydrate-bearing sediment. The model is validated by experimental data with various hydrate saturation, temperature and pressure conditions. The deformation induced by partial dissociation was studied through depressurization tests under constant effective stress. The reduction in gas pressure within the hydrate stability field indeed caused sediment deformation. The dissociation-induced deformation can be reasonably estimated as the difference in volume between hydrate-bearing and hydrate-free sediments from the compression curves.

Uncertainty in measuring the role of climate change on debris-flow triggering on volcanoes - bulk-density, temperature and moisture analysis at Unzen Volcano (Japan)

As climate change creeps into the 21st century, the intensity of debris flows due to heavy and concentrated rainfall has increased in mountainous regions of Japan and East Asia. However, the relationship between climate change and an increase in debris flows is likely to be non-linear. Rainwater infiltrates more quickly into porous material, and the lack of vegetation cover increases evaporation and the temperature of surface sediments. In addition, periodic gully collapses bring fresh layers of porous material that increase the distance between the surface and the vadose zone. Therefore, to understand the relationship between volcanic debris flows (or lahars), parameters such as density, porosity, temperature, and moisture retention must be captured in detail in both time and space. The aim of this paper is to assess the role of loose, coarse, and porous sediments in lahar triggering. The present study was conducted at Unzen volcano in the Tansandani Guly between 31 May and 1 June 2023, 30 years after the last eruption. The dacite material is composed of a matrix of sand and coarse sand mixed with larger fractions and blocks, therefore traditional density measurement methods could not be applied, and a photogrammetric based method was used. In the field, sets of SfM-MVS photographs were taken before and after digging a hole in the sediments so that the measured mass could be compared to the volume of the hole in the sediments. After the sediments were dried, the dry and wet density, bulk density and porosity of the sample were calculated. When compared to the temperature data collected in the field, the following results were obtained: (1) The porosity of the volcanic material was highest in the lower reaches, followed by the upper reaches, and lowest in the middle reaches. This may be because fine sand washed out of the upstream area by rainfall is currently stored in the midstream area, which may facilitate debris flow generation. In addition, the downstream area has a high porosity, which may be due to the surrounding vegetation preventing the influx of new fine sand from the channel wall. (2) Because of the higher porosity and the lack of organic matter and vegetation cover, the increase in temperature acts more directly on the decrease in water content than in mountainous areas. Consequently, empirical equations for the potential for mudslides in volcanic areas with respect to surface temperature and soil moisture need to be developed for hazard and disaster risk management purposes.

Seasonal drivers and patterns of sediment temperatures in a New England salt marsh

AbstractSalt marshes are globally important ecosystems and thus their resilience to climate change holds societal importance. To date, studies addressing salt marsh responses to climate change have focused on sea‐level rise and biogeochemical feedbacks with increasing inundation. Less is known about how salt marsh sediment temperatures, which impact physical, biological, and chemical ecosystem processes, will respond to climate change. In this study, we present multi‐depth sediment temperature and porewater level data from low‐, mid‐, and high‐marsh sites at a New England salt marsh for a 1‐year period and investigate how salt marsh sediment temperatures respond to atmospheric and oceanic forcing. We use spectral analyses to identify the frequencies at which sediment temperatures vary and link the temperature variations to specific forcing mechanisms. We find that all sites across the marsh responded to air temperature with roughly equal amplitude whereas the responses to radiative heating and ocean tides varied spatially. The high‐marsh site is more sensitive to radiative heating than the mid‐ and low‐marsh sites. The low‐marsh is affected by tidal processes and inundation whereas the high‐ and mid‐marsh sites are not. In addition, we find that the bulk thermal diffusivity of the saturated sediments decreases with distance from the tidal channel. These factors contribute to considerable temporal and spatial variability in sediment temperatures with elevation, distance from the tidal channel, and time of year (season) being most important.

The influence of in-stream structures on temperature dynamics via induced hyporheic exchange

In-stream restoration structures, such as woody weirs, are intended to enhance hyporheic exchange, thus altering temperature dynamics in restored reaches. However, the relative effects of various in-stream structure types and streambed settings (i.e. the size, spacing, number and sediment hydraulic conductivity) remain poorly understood. Here, we conducted a field experiment with a channel-spanning weir in a stream to study its impact on hyporheic exchange and streambed and surface water temperatures. We subsequently applied numerical models to explore the effects of height, spacing and number of weirs and sediment permeability on hyporheic exchange and thermal distribution characteristics. Based on the field experiments and numerical models, the results showed that the addition of a weir enhanced hyporheic exchange and produced a characteristic temperature heterogeneity pattern in sediment that varied with height, spacing, number of weirs and sediment permeability. The spatial extent and rate of hyporheic exchange and temperature heterogeneity near the weir primarily increased with weir height and sediment permeability. With increasing spacing, the exchange flux and thermally heterogenous area increased, but the exchange intensity decreased, and the sediment temperature readily stabilized. With increasing number of weirs, the exchange flux first decreased and then increased, and the region exhibiting temperature heterogeneity increased. The interaction between the introduction of in-stream structures introduction and streambed settings must be understood to improve vertical connectivity in rivers, increase the thermal heterogeneity in the hyporheic zone and create localized but potentially valuable thermal refuge areas.

Meiofauna community structure in the Yellow Sea Cold Water Mass and non‐Yellow Sea Cold Water Mass

This study investigated meiofaunal community structure over a large area of the Yellow Sea and East China Sea for 45 stations from 2021 to 2022. Meiofaunal densities were 34 ± 15–1610 ± 378 ind./10 cm2. Nematodes (67.3%) were the most dominant taxa, followed by benthic foraminifera (12.0%) and benthic harpacticoids (8.8%). The study was conducted in various sedimentary environments. Median grain size, water depth, and environmental characteristics (TOC, TN, Hg, Sr) were identified as influencing meiofauna community structure. The Yellow Sea and East China Sea, where this study was conducted, contain a variety of currents and the Yellow Sea Cold Water Mass (YSCWM) in the central waters. YSCWM and non-YSCWM areas differed in their meiofauna community structure. Even within the YSCWM region, we found differences in benthic community structure due to a variety of environmental factors. The meiofaunal taxon most affected by Sediment temperature was benthic foraminifera. Identifying foraminifera in paleo-oceanographic environments can indicate past expansion and contraction of YSCWM. Meiofauna have been used to monitor changes in marine ecosystems due to their sensitivity to changes in the surrounding environment. Meiofauna index (N/C ratio, MPI) were identified as appropriate for use in the study area.

Dynamics of benthic microeukaryotic communities in a mangrove wetland invaded by Spartina alterniflora: Effects of vegetation, seasonality, and sediment depth

Benthic microeukaryotes are crucial mediators of biogeochemical cycles in coastal wetland ecosystems, yet their spatial and temporal variability remains poorly understood. This study delineates the diversity patterns of benthic microeukaryotes in a Spartina alterniflora-invaded mangrove ecosystem in Fujian, China. Using high-throughput sequencing of 18S rRNA gene transcripts, we identified the influences of vegetation, seasonality, and sediment depth on microeukaryotic communities. We discovered that vegetation cover significantly affects community composition, primarily driven by nutrient concentrations and pH. The community structure of microeukaryotes varied seasonally and vertically, correlating with changes in sediment temperature, pH, salinity, and fucoxanthin concentration. Notably, invasive Spartina alterniflora habitats showed enhanced heterotrophic interactions, suggesting that invasive species can reshape benthic microeukaryotic co-occurrence patterns. Seasonal co-occurrence patterns revealed dominant Bacillariophyta assemblages exhibited distinct network modules enriched in the cold (spring) and warm (summer and fall) seasons, respectively, which indicated potential ecological niche differentiation. Our findings reveal the complex relationships between environmental factors and benthic microeukaryotic diversity, offering insights into microbial responses to natural and invasive vegetation influences.

Sediment temperature characteristics and their relation to distribution patterns of two sentinel crab species in intertidal flats in western Japan

In the context of global climate change, monitoring focused on temperature and benthic animals in intertidal flats and the development of metrics to assess climate change and ecosystem responses are essential for a sustainable society. However, few studies have assessed the relationship between intertidal sediment temperature and the distribution of benthic animals. To address this gap, in the present study, intertidal sediment temperatures were observed in 12 intertidal flats in 11 survey areas over 335 days, from October 2, 2019, to August 31, 2020, using water temperature data loggers. The characteristics of intertidal sediment temperatures were variable among the survey areas, and a correlation analysis suggested that such characteristics are possibly influenced by various spatial-scale factors, such as geographical, basin, and habitat scales. Furthermore, two sentinel crab species, Macrophthalmus japonicus and Macrophthalmus banzai were collected, and the number of wintering individuals of each species was estimated based on their carapace width to analyze the changes in abundances of the two species in each survey area. The results show that the number of days with daily minimum temperature ≥ 19 °C was the factor that influenced the abundance rate, suggesting that M. japonicus and M. banzai populations may decrease and increase, respectively, according to future climate change in Japan. Our findings emphasize the importance of long-term monitoring of sediment temperatures and benthic animals in intertidal flats to evaluate the influence of future climate change.

Cold Seeps and Heat Flow: Gas Hydrate Provinces Offshore Sakhalin Island

Gas hydrates were found in bottom sediments on the western slope of the Kuril Basin from the side of the Terpeniya Gulf (Okhotsk Sea) at 1020 m depths during expeditions in 2012 and 2013. However, on the eastern slope of the Tatar Strait, gas hydrates were sampled at an unusually shallow 322 m depth. During our research, we identified gas hydrate provinces based on both bottom water and sediment temperature measurement data and heat flow, earthquake, cold seep and sea current data analyses. These provinces have similar hydrological regimes, providing suitable temperature conditions for the existence of gas hydrates, to those at a 322 m depth in the Tatar Strait (Japan Sea) and at 725 and 1020 m depths on the slope of the Kuril Basin (Okhotsk Sea).

The crystal orientation of THF clathrates in nano-confinement by in situ polarized Raman spectroscopy.

Gas hydrates form at high pressure and low temperatures in marine sediments and permafrost regions of the earth. Despite forming in nanoporous structures, gas hydrates have been extensively studied only in bulk. Understanding nucleation and growth of gas hydrates in nonporous confinement can help create ways for storage and utilization as a future energy source. Herein, we introduce a new method for studying crystal orientation/tilt during tetrahydrofuran (THF) hydrate crystallization under the influence of nano-confinement using polarized Raman spectroscopy. Uniform cylindrical nanometer size pores of anodic aluminum oxide (AAO) are used as a model nano-confinement, and hydrate experiments are performed in a glass microsystem for control of the flash hydrate nucleation kinetics and analysis via in situ polarized Raman spectroscopy. The average THF hydrate crystal tilt of 56 ± 1° and 30.5 ± 0.5° were observed for the 20 nm and 40 nm diameter pores, respectively. Crystal tilt observed in 20 and 40-nanometer-size pores was proportional to the pore diameter, resulting in lower tilt relative to the axis of the confinement at larger diameter pores. The results indicate that the hydrates nucleation and growth mechanism can depend on the nanoconfinement size. A 1.6 ± 0.01 °C to 1.8 ± 0.01 °C depression in melting point compared to the bulk is predicted using the Gibbs-Thomson equation as a direct effect of nucleation in confinement on the hydrate properties.

Microbial methane formation in deep aquifers associated with the sediment burial history at a coastal site

Abstract. Elucidating the mechanisms underlying microbial methane formation in subsurface environments is essential to understanding the global carbon cycle. This study examined how microbial methane formation (i.e., methanogenesis) occurs in natural-gas-bearing sedimentary aquifers throughout the sediment burial history. Water samples collected from six aquifers of different depths exhibited ascending vertical gradients in salinity from brine to fresh water and in temperature from mesophilic to psychrophilic conditions. Analyses of gas and water isotopic ratios and microbial communities indicated the predominance of methanogenesis via CO2 reduction. However, the hydrogen isotopic ratio of water changed along the depth and salinity gradient, whereas the ratio of methane changed little, suggesting that in situ methanogenesis in shallow sediments does not significantly contribute to methane in the aquifers. The population of methane-producing microorganisms (methanogens) was highest in the deepest saline aquifers, where the water temperature, salinity, and total organic carbon content of the adjacent mud sediments were the highest. Cultivation of the dominant hydrogenotrophic methanogens in the aquifers showed that the methanogenesis rate was maximized at the temperature corresponding to that of the deepest aquifer. These results suggest that high-temperature conditions in deeply buried sediments are associated with enhanced in situ methanogenesis and that methane that forms in the deepest aquifer migrates upward into the shallower aquifers by diffusion.