Secondary Metabolite Compounds Research Articles

Uji Bioaktivitas Fraksi Etanol Ekstrak Kulit Buah Jeruk Purut Citrus hystrix Sebagai Anti Bakteri Staphilococus aureus dan Escherecia coli

Plants have several ways to protect themselves from bacteria, one of which is by producing compounds that are toxic or repellent to bacteria. Compounds produced by plants are known as secondary metabolite compounds which originate from secondary metabolic processes. Several secondary metabolite compounds such as flavonoids, alkaloids, saponins, tannins, steroids and terpenoids are currently widely used as antibacterials. One of the plants that has the potential to be developed as a herbal medicine is kaffir lime leaves. Citrus hystrix is ​​the part of the plant most often used by the public. Both for cooking and traditional medicine purposes. In this research, it is hoped that lime peel extract can act as an antibacterial for Escherichia coli and staphylococcus aereus, so that it can be used as a herbal plant for diseases caused by these two bacteria. In the research, the concentrations used for each test bacteria were 50%; 25%; 12.5%. in observing S. aureus bacteria 1x24 hours with a concentration of 50%, the size of the inhibitory zone was 16 mm, the 25% concentration was 11 mm and the 12.5% ​​concentration was 9 mm. Meanwhile, E. Coli bacteria did not show any reaction to the heruk peel extract.

Cytotoxic of Usnic Acid Isolated from Ramalina sp.

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane: EtOAc, EtOAc, EtOAc: MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane: EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyls (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Com-pound 1 caused a 67.06% decrease in MCF7 viability.

Neuroprotective Effect of Colocasia esculenta Var. Mentawai Corm Flour High-Fat Diet Fed Mice

The transformation of our era, resulting in a change in dietary habits towards a higher intake of fatty foods, presents a worldwide health issue. Among these challenges is neurodegeneration, which leads to cognitive impairment. It is imperative to seek alternative solutions rooted in nature to address the limitations associated with non-natural treatment methods. This entails harnessing the properties of secondary metabolite compounds found in plants, such as Colocasia esculenta Var. Mentawai. This research aims to assess the efficacy of C. esculenta Var. Mentawai corm as neuroprotective agents in mitigating CNS damage and preventing cognitive decline associated with neurodegeneration. Daily administration of a high-fat diet and a mixture of taro flour is conducted on young adult male mice for a duration of 60 days. Furthermore, analysis of the neurocognitive ability of mice, determination of malondialdehyde levels, and observation of histopathological structures on brain tissue were carried out. The results showed that the group of mice fed with taro flour mixture effectively showed a positive impact on maintaining neurocognitive abilities and histopathological structure of brain tissue against neurodegeneration (p

Socialization of The Use of Sisik Naga Leaves (Drymoglossum Piloselloides) Medicinal Plant as An Antibacterial for The Lubuk Pakam Pekan

In Indonesia, one type of plant that is often found is Drymogglossum Piloselloides, or better known as dragon scale leaves. These leaves are included in the category of herbal plants or traditional medicines which are rich in anti-inflammatory, anti-oxidant and anti-cancer compounds. Not only this, secondary metabolite compounds contain various other forms, namely flavonoids, tannins and saponins, which are believed to be antibacterial, to inhibit the growth of bacteria that cause acne, including Staphylococcus epidermidis, which is a member of the genus Gram-positive staphylococcus. In this community service activity, the aim is to provide information about the role and benefits of dragon scale leaves which can be an antibacterial agent for the wider community. In the service program carried out through socialization media using lecture, question and answer and implantation methods with the participation of 15 people in Lubuk Pakam. In accordance with the PkM program that has been produced, a conclusion was obtained regarding wider community insight and knowledge about dragon scale leaves which can be used for antibacterial purposes, in this case proven by the increase in the posttest score which was compared with the pretest score. The average increase in knowledge of PkM participants was obtained with a value of 28.4. Not only that, the maximum posttest score was obtained with a score of 85, in contrast to the lowest posttest score, namely 55 if a comparison was made with the pretest that had been carried out previously.

Plectranthus scutellarioides (L.) R.Br. Leaf Extract as Sunscreen, Skin Lightening, and Antiaging

Plectranthus scutellaroides is a plant with attractive colored leaves. It contains secondary metabolite compounds such as flavonoids and phenolics, which provide skin protection against ultraviolet rays from the sun. The research aims to investigate the activity of P. scutellarioides leaf extract from various variants and different solvents on sunscreen, skin lightening, and anti aging activities. The best extract is formulated in gel dosage form. Each P. scutellaroides leaf was extracted using ethanol, ethyl acetate, and hexane as solvents. The obtained extract was assessed for Sun Protecting Factor (SPF), UVA Protection grade (PA), antioxidant activity, and inhibition of tyrosinase, collagenase, elastase, and hyaluronidase. Determination was conducted in vitro using spectrophotometric methods. The highest sunscreen activity was observed in the ethanol extract Va (Va-ET), which exhibited SPF and PA values of 25.618±0.265, and 0.681±0.007 (star 3), respectively at a concentration of 100 ug/mL. The most significant skin-lightening activity was observed in the ethanol extract Vc (Vc-ET), with an IC50 value inhibiting tyrosinase of 39.059 ug/mL. Regarding the anti-aging activity of the extracts, as determined by their antioxidant activity and inhibition of collagenase, elastase, and hyaluronidase, the most promising extracts were obtained from ethanol extract Va (Va-ET) and ethyl acetate extract Va (Va-EA), with IC50 values of 79.734, 76.838, 143.384, and 122.467 ug/ mL, respectively. The Va-ET was formulated into a gel dosage form, and there was a significant effect of the gelling agent on the SPF value of the extract after formulation (p < 0.05). All extracts exhibit activity as sunscreen, skin lightening, and anti-aging, with the Va-ET showing the highest efficacy. Gelling agents significantly influence the SPF value of extracts after formulation into the gel dosage form. Among them, F3, formulated from the Va-ET of P. scutellaroides using Carbopol-940 as a gelling agent, exhibits the highest efficacy.

ISOLATION OF ETHYL TRANS-P-METHOXYCINNAMATE FROM Kaempferia galanga L. RHIZOMES BY USING N-HEXANE

Kaempferia galanga L. rhizomes contain ethyl trans-p-methoxycinnamate as a major secondary metabolite compound and biomarker. Various extraction methods with different solvents can isolate ethyl trans-p-methoxycinnamate with potential bioactivities. This paper reported the isolation of ethyl trans-p-methoxycinnamate from two varieties of K. galanga L. rhizomes by maceration in n-hexane. The small and large varieties of K. galanga samples produced ethyl trans-p-methoxycinnamate in 1.43% and 1.84% yields, higher than other methods and polar solvents of previous research. Structural elucidation of the obtained ethyl trans-p-methoxycinnamate was performed by FTIR, MS, and 1H NMR spectroscopies.

ANALYSIS OF TOTAL FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITY ASSAY OF GUAVA VARIETY CRYSTAL (Psidium guajava L.) LEAVES EXTRACT

The study investigates the antioxidant activity and total flavonoid compounds in leaves crystal guava, a plant rich in vitamin C and high in antioxidant activity. The research involved maceration extraction of leaves cystal guava using methanol solvent, then fractionation with ethyl acetate and n-hexane solvents in stages to obtain extracts methanol, ethyl acetat and n-hexane; thereafter extracts were subjected to phytochemical screening, total flavonoid content determination, and antioxidant activity using the DPPH method. The study found secondary metabolite compounds which is found in leaves crystal guava such as flavonoids, alkaloids, steroids, terpenoids, saponins and tannins; that ethyl acetate extract had the highest flavonoid content of 171.91 mg/L and highest antioxidant activity with IC50 value of 9.59 ppm, followed by n-hexane and methanol extracts.

DEVELOPMENT OF A REFERENCE BOOK BASED ON THE IDENTIFICATION DATA OF MEDICINAL PLANTS IN THE COMMUNITY OF THE SULTANATE OF MOLOKU KIERAHA, NORTH MALUKU, INDONESIA

The aim of this research is to develop teaching materials in the form of a reference book based on studies of plant medicinal identification in the people of the Moloku Kieraha sultanate. The research involved students in semester 2 of the Unkhair Postgraduate Biology Education program for the 2023/2024 academic year. The study used interview sheets, observation sheets, validation sheets, and response questionnaires for data collection, and validation sheet and product trial analysis for analysis. The results of the research identifies 36 medicinal plants used by the Serawai tribe community in Torano Village, Central Ternate District, Maluku Province. Phytochemical analysis reveals that these plants contain secondary metabolite compounds, including tannins, alkaloids, saponins, flavonoids, terpenoids, and steroids. Students showed a positive response to the medicinal plant reference book that had been developed, with an interest level reaching 98%. Therefore, teaching materials are needed to guide students in conducting phytochemical tests to determine the content, benefits, and techniques of phytochemical screening in natural materials.

Phytochemical screening of three extraction process of Calotropis gigantea

Abstract Calotropis gigantea is a flowering plant belonging to the genus Calotropis of the family Asclepiadaceae. Many pharmacological activities of this plant show potential in human therapy. In this study, an investigation was conducted on the three extracted process namely n-hexane extract, ethyl acetate extract and ethanolic extract of C. gigantea collected from a coastline at Alue Naga beach, Banda Aceh, Indonesia. Percent yield of C. gigantea leaf extract was mostly produced by ethanol solvent compared to ethyl acetate and n-hexane solvents, with total 8.25 grams (4.12%), 5.62 grams (2.80%), and 4.51 grams (2.25%), respectively. The results of phytochemical screening explained that the ethanol extract of C. gigantea leaves had abundant secondary metabolite compounds, compared to the n-hexane extract, only the steroid compounds were detected. The ethyl acetate extract of C. gigantea leaves showed the presence of flavonoids, triterpenoids, tannins, and phenolic compounds, but no saponins and alkaloids were found. These observations underscore the exciting potential of C. gigantea as a promising reservoir of flora rich in alkaloids, flavonoids, phenolics, tannins, saponins, and steroid compounds, derived from the coast so that it can be used as a drug candidate.

Identification of secondary metabolite compounds in n-hexane extract of noni (Morinda citrifolia Linn) leaves through phytochemical test

Abstract Noni (Morinda citrifolia Linn) is one of the plants with a high antioxidant content in its roots, leaves, and fruit. Noni leaf extract contains numerous active compounds, including flavonoids, anthraquinones, sterols, terpenoids, alkaloids, tannins, ascorbic acid, beta carotene, and prexeronin. The aim of this study was to use phytochemical tests to identify the active compounds in n-hexane extracts of noni leaves. This test begins with extracting noni leaves using n-hexane solvent. The resulting extracts were subjected to phytochemical testing including alkaloid, flavonoid, steroid, terpenoid, tannin, and saponin tests. The results of this test showed that the n-hexane extract of noni leaves contained only terpenoids and steroids. As a result, it can be concluded that these two compounds can react with non-polar solvents such as n-hexane.

Phytochemical screening of three extraction process of Calotropis gigantea

Abstract Calotropis gigantea is a flowering plant belonging to the genus Calotropis of the family Asclepiadaceae. Many pharmacological activities of this plant show potential in human therapy. In this study, an investigation was conducted on the three extracted process namely n-hexane extract, ethyl acetate extract and ethanolic extract of C. gigantea collected from a coastline at Alue Naga beach, Banda Aceh, Indonesia. Percent yield of C. gigantea leaf extract was mostly produced by ethanol solvent compared to ethyl acetate and n-hexane solvents, with total 8.25 grams (4.12%), 5.62 grams (2.80%), and 4.51 grams (2.25%), respectively. The results of phytochemical screening explained that the ethanol extract of C. gigantea leaves had abundant secondary metabolite compounds, compared to the n-hexane extract, only the steroid compounds were detected. The ethyl acetate extract of C. gigantea leaves showed the presence of flavonoids, triterpenoids, tannins, and phenolic compounds, but no saponins and alkaloids were found. These observations underscore the exciting potential of C. gigantea as a promising reservoir of flora rich in alkaloids, flavonoids, phenolics, tannins, saponins, and steroid compounds, derived from the coast so that it can be used as a drug candidate.

Identification of secondary metabolite compounds in n-hexane extract of noni (Morinda citrifolia Linn) leaves through phytochemical test

Abstract Noni (Morinda citrifolia Linn) is one of the plants with a high antioxidant content in its roots, leaves, and fruit. Noni leaf extract contains numerous active compounds, including flavonoids, anthraquinones, sterols, terpenoids, alkaloids, tannins, ascorbic acid, beta carotene, and prexeronin. The aim of this study was to use phytochemical tests to identify the active compounds in n-hexane extracts of noni leaves. This test begins with extracting noni leaves using n-hexane solvent. The resulting extracts were subjected to phytochemical testing including alkaloid, flavonoid, steroid, terpenoid, tannin, and saponin tests. The results of this test showed that the n-hexane extract of noni leaves contained only terpenoids and steroids. As a result, it can be concluded that these two compounds can react with non-polar solvents such as n-hexane.

Phytochemical Screening of Ethanol Extract of Tarap Fruit Seeds (Artocarpus odoratissimus)

Tarap fruit (Artocarpus odoratissimus), also known as "terap", is a type of fruit that grows in various tropical regions in Southeast Asia. because almost every part of the fruit has pharmacological properties, it is often used as an ingredient in traditional medicine. The plant has been part of many different cultures for a long time, used as a food source, and is widely used to help improve health. This study aims to conduct phytochemical screening on tarap fruit seed extract to identify the secondary metabolite compounds contained in it with a focus on compounds such as polyphenols, alkaloids, tannins, saponins, steroids, and terpenoids. This study uses a qualitative experimental method to analyze phytochemistry by color test using various reagents from tarap fruit seed extract, to identify secondary metabolite compounds in the plant. Tarap fruit seed samples were obtained from local fruit sellers in Tarakan. Extraction of tarap fruit seeds was carried out using 96% ethanol with maceration technique, then examined the content of polyphenols, alkaloids, tannins, saponins, steroids and terpenoids. The results showed the presence of several secondary metabolites in tarap fruit seed extract, proving the content of polyphenols, alkaloids, saponins, and terpenoids, while tannins and steroids could not be detected. The conclusion is that the ethanol extract of tarap fruit seeds (Artocarpus odoratissimus) contains polyphenols, alkaloids, saponins, and terpenoids.

Hytochemical Analysis and Determination of MIC and MFC of Cacao Leaves Extract (Theobroma cacao L.) against Malassezia furfur

Pityriasis versicolor is a disease caused by Malassezia furfur. One of the plants that can potentially act as antifungal is cacao leaves (Theobroma cacao L.), which contain several secondary metabolite compounds, including alkaloids, flavonoids, tannins, quinones, terpenoids, and saponins. This study aims to determine the phytochemical analysis and the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of cacao leaves ethanolic extract against M. furfur. Extracts were obtained by maceration using 70% ethanol and phytochemicals analysis using Herborne’s method. Furthermore, the extracts were made into concentrations’ variations of 3.125%; 6.25%; 12.5%; 25%; 50%; and 100% for testing antifungal activity, with 10% DMSO as negative control and 2% Ketoconazole and Itraconazole as positive controls. MIC was performed with liquid macrodilution method, and MFC was performed with spread method. The results of phytochemical analysis showed that the extract contained alkaloids, flavonoids, phenols, tannins, quinones, terpenoids and saponins. MIC value was obtained at concentration of 50% and MFC value was obtained at 100%. Cacao leaves ethanolic extract with concentration of 50% can inhibit M. furfur’s growth and with 100% can eliminate M. furfur.

Antibacterial activity of Kemloko fruit ethanol extract (Phyllanthus emblica L.) against Staphylococcus aureus and MRSA bacteria

Background: Indonesia is a tropical country, so the prevalence of infectious diseases caused by bacteria remains high. On the other hand, the high use of antibacterial ingredients in Indonesia causes bacterial resistance to existing antibacterial drugs. One of the herbal plants that some people widely use is Kemloko fruit. This study aimed to determine the activity of Kemloko fruit (Phyllanthus emblica L.) ethanol extract against the growth of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) bacteria. Methods: Simplicia of Kemloko fruit were tested for secondary metabolites and characterization test of the simplicia. The antibacterial activity test was carried out using the diffusion method, where the disc paper was immersed in the test solution with the ratio of the test concentration. Results: The results of phytochemical screening showed that the secondary metabolite compounds of Kemloko fruit are alkaloids, flavonoids, steroids, tannins, saponins, and glycosides. Antibacterial activity of ethanol extract of Kemloko fruit with agar diffusion method effectively inhibits S. aureus bacteria, namely at a concentration of 80 mg/ml, the inhibition zone is 14.1 mm, and at a concentration of 100 mg/ml, MRSA bacteria obtained an inhibition zone of 14.3 mm. Conclusions: The ethanol extract inhibits the growth of S. aureus and MRSA.

The Antidiabetic Potential of Ruellia tuberosa L.

Diabetes mellitus (DM) is a metabolic condition characterized by insufficient insulin production or resistance to insulin, resulting in elevated blood sugar levels. Several synthetic medications, such as Acarbose, Metformin, Glibenclamide, Miglitol, and Voglibose, are presently employed to manage high blood sugar levels. However, these drugs have many side effects, causing some mild to severe adverse effects, including gastrointestinal symptoms, nausea, and vomiting. Hence, it is crucial to research natural products as promising antidiabetic alternatives. This study aimed to provide a comprehensive overview of the potential of Ruellia tuberosa L. as an antidiabetic drug candidate based on the secondary metabolite compounds contained in it. The literature review process involved searching specific keywords in various databases, including Google Scholar, the GARUDA portal, ScienceDirect, PubMed, and DOAJ. All incomplete, duplicates, and pay-access articles were filtered out, and inclusion criteria were applied. The result of this study shows that Ruellia tuberosa L. secondary metabolite compounds are alkaloids, amino acids, ascorbic acids, carbohydrates, flavonoids, glycosides, phenolics, quinoline, saponins, steroids, sterols, tannins, and terpenoids. In silico molecular docking analysis and in vivo testing of Ruellia tuberosa L. extract on streptozotocin (STZ)-induced diabetic Wistar rats show that Ruellia tuberosa L. has the potential to be developed as an antidiabetic alternative drug.

Antimicrobial Activity Test of Ethanol Extract of Senggani Leaves (Melastoma malabraticum L) Against Propionibacterium Acnes and Staphylococcus Epidermidis

Senggani leaves are a plant that can be found in Indonesia, including hilly locations. The people of Marendal, Patumbak subdistrict, believe that senggani leaves may heal minor skin wounds. The goal of this study was to identify the class of secondary metabolite chemical compounds and their antibacterial efficacy against the microorganisms Propionibacterium acnes and Staphylococcus epidermidis at 5, 10, and 20% concentrations. The ethanol extract of senggani leaves contains secondary metabolite compounds with antibacterial activity. According to the antibacterial test results, a 20% ethanol extract of senggani leaves is effective against Propionibacterium with a diameter of 10.7 mm, a 10% in the medium category with a diameter of 9.27 mm, and a 5% in the medium category with a diameter of 8.8 mm. Meanwhile, the diameter of Staphylococcus epidermidis at a 20% was 8.77 mm in the medium category, 8.37 mm at 10%, and 8.6 mm at a 5% concentration. Keywords: Propionibacterium acnes, Senggani leaves, Staphylococcus epidermidis

PELATIHAN PEMBUATAN TEH CELUP DARI TANAMAN BUNGA TELANG (Clitoria ternatea) DI SMK PGRI 2 CIMAHI

Tea is a drink that is very common in our daily lives which can be used as a health drink for the body. Tea products are not only made from the tops of tea plant leaves, but can be produced from other leaves such as butterfly pea flowers (Clitoria ternatea). Butterfly pea flowers contain secondary metabolite compounds such as phenols, flavonoids, alkaloids and anthocyanins. These secondary metabolites make telang flowers useful for treating premature aging, cancer, digestive disorders and cardiovascular diseases. The aim of this community service is to introduce tea bags made from butterfly pea flowers. The activity methods used are demonstrations, lectures and discussions. The materials and equipment used are butterfly pea flowers, lemon, warm water, ice cubes, honey and spoons and glasses. Participants who took part in this activity were students of SMK PGRI 2 Cimahi. The results of the training activities that have been carried out are that students at SMK PGRI 2 Cimahi can understand the process of making and using butterfly pea flowers as tea bags and produce healthy tea drinks with flavors that students like. The conclusion of this training is that students can learn about the use of natural ingredients such as butterfly pea flowers as tea bags which have various health benefits.

Secondary Metabolite Profile in Mature and Old Leaves of Four Piper Species: Forest Betel (Piper aduncum L.), Red Betel (Piper crocatum Ruiz & Pav.), Javanese Chili Betel (Piper retrofractum Vahl.), and Green Betel (Piper betle L.)

Piper species is a potential medicinal plant, empirically known for its effectiveness in curing various diseases, particularly in Indonesia. Therefore, this research aimed to evaluate the similarities and differences in the profile of secondary metabolite compounds in mature and old leaves of four Piper species using Gas Chromatography-Mass Spectrometry (GC-MS). The analysis was also carried out to identify the specific compounds found in each species at different leaf development stages. Samples used were mature and old leaves from four species of Piper, namely forest betel (Piper aduncum L.) (PA), red betel (Piper crocatum Ruiz & Pav.) (PC), Javanese chili betel (Piper retrofractum Vahl.) (PR), and green betel (Piper betle L.) (PB). Subsequently, samples were extracted using ethanol solvent and secondary metabolite profile was detected through GC-MS. A total of 40 secondary metabolite compounds were found in mature and old leaves of four species. The results showed that alkaloid content contributed 25% of the total compounds detected, while fatty acids yielded the largest portion (27.5%). Based on PCA score plot analysis, a significant grouping of secondary metabolite compounds was observed in all species, where PC was categorized separately on the right, and the other species were on the left. Several specific compounds were also found only in one species and not in others. Similar to mature and old leaves, some compounds were discovered in one of the developmental phases. Dillapiole and hexadecane were only found in PA, both in mature and old leaves; germacrene A, eugenol, and chavicol were found in mature and old leaves of PB, but beta-selinene, delta-guaiene, and linolelaidic acid were only found in mature leaves. In PC, there were specific compounds namely benzofuran, myrcene, and 1,6,10-dodecatrien that were found in mature and old leaves. Besides that, trans-ocimene and alpha-bisabolol were only found in old leaves of PC. In PR, only tetracosane was found in mature leaves, while piperidine, octanodecanoic acid, and benzylmalonic acid were found in old leaves.

Potential of siam weed extract in combination with Trichoderma harzianum to stimulate seed germination of Coffea arabica

The Arabica coffee (Coffea arabica) beans feature a solid and thick seed shape that obstructs the germination process. Seed germination is an important feature of coffee plant cultivation and significantly impacts on the growth and development of the coffee plant. Siam weed (Chromolaena odorata L.) has the potential to be used as an organic fertilizer due to its nutrients and secondary metabolite compounds which can help accelerate plant growth. The optimal use of organic fertilizer can be achieved by using Trichoderma harzianum. This research aims to determine the effect and optimal concentration of Siam weed extract (Chromolaena odorata L.) and Trichoderma harzianum on the germination of Arabica coffee seeds. The research used a factorial Completely Randomized Design (CRD) in the form of siam weed extract concentrations of 8 ppm and 16 ppm and concentrations of Trichoderma harzianum of 5 ppm, 10 ppm, 15 ppm, and 20 ppm. The research finding indicated that the C2T1 treatment had the best results in terms of germination, growth speed, growth synchrony, hypocotyl length, and number of leaves on coffee plants. Meanwhile, the C2T4 treatment had the highest chlorophyll content.