Seasonal Senescence Research Articles

Kelp dissolved organic carbon release is seasonal and annually enhanced during senescence.

Macroalgae influence local and global biogeochemical cycles through their production of dissolved organic carbon (DOC). Yet, data remain scarce and annualized estimates are typically based on high growth periods without considering seasonal variability. Although the mechanisms of active exudation and passive leakage need clarifying, ecophysiological stress is known to enhance DOC release. Therefore, DOC leakage from seasonally senescent macroalgae may be overlooked. This study focuses on the annual kelp Saccharina japonica var. religiosa (class Phaeophyceae) from Oshoro Bay, Hokkaido, Japan. Three years (2020-2022) of seasonal data were collected and analyzed, with least squares mean DOC release rates established for kelp (n = 88) across 16 incubation experiments (t ≥ 4 d, DOC samples ≥1 · d-1) under different photosynthetically active radiation (PAR) treatments (200, 400, 1200, or 1500 μmol photons · m-2 · s-1). Differences in PAR, dry weight biomass (g DW), sea surface temperature, or salinity could not explain DOC release-rate variability, which was high between individual kelp. Instead, there were significant intra-annual differences, with mean DOC release rates (mg C · g-1 DW · d-1 ± standard error between n kelp) higher during the autumn "late decay" period (0.71 ± 0.10, n = 27) compared to the winter "early growth" period (0.14 ± 0.025, n = 10) and summer "early decay" period (0.25 ± 0.050, n = 24). This relationship between seasonal senescence and macroalgal DOC release is further evidence that long-term, place-based studies of DOC dynamics are essential and that global extrapolations are premature.

Variation in the timing and duration of autumn leaf phenology among temperate deciduous trees, native shrubs and non-native shrubs.

The timing and duration of autumn leaf phenology marks important transitions in temperate deciduous forests, such as, start of senescence, declining productivity and changing nutrient cycling. Phenological research on temperate deciduous forests typically focuses on upper canopy trees, overlooking the contribution of other plant functional groups like shrubs. Yet shrubs tend to remain green longer than trees, while non-native shrubs, in particular, tend to exhibit an extended growing season that confers a competitive advantage over native shrubs. We monitored leaf senescence and leaf fall (2017-2020) of trees and shrubs (native and non-native) in an urban woodland fragment in Wisconsin, USA. Our findings revealed that, the start of leaf senescence did not differ significantly between vegetation groups, but leaf fall started (DOY 273) two weeks later in shrubs. Non-native shrubs exhibited a considerably delayed start (DOY 262) and end of leaf senescence (DOY 300), with leaf-fall ending (DOY 315) nearly four weeks later than native shrubs and trees. Overall, the duration of the autumn phenological season was longer for non-native shrubs than either native shrubs or trees. Comparison of the timing of spring phenophases with the start and end of leaf senescence revealed that when spring phenology in trees starts later in the season senescence also starts later and ends earlier. The opposite pattern was observed in native shrubs. In conclusion, understanding the contributions of plant functional groups to overall forest phenology requires future investigation to ensure accurate predictions of future ecosystem productivity and help address discrepancies with remote sensing phenometrics.

Zoysiagrass (Zoysia spp.) accessions differ in nitrogen cycling during a simulated seasonal senescence

AbstractZoysiagrass is a valuable low maintenance warm‐season turfgrass. Nitrogen management is one of the most important management practices that affect colour retention and winter hardiness. However, the N cycling during seasonal senescence and its interaction with low temperature acclimation are not well‐understood. The objective of this experiment was to understand the initiation of senescence as affected by environmental signals and the contribution of chlorophyll and protein degradation to N metabolites. Two commercial cultivars (‘Meyer’ and ‘Zenith’), seven wild accessions of Z. japonica, one wild accession of Z. matrella, and one wild accession of Z. pacifica from China were tested. Mature plants were exposed to four sequential environmental conditions to simulate fall shortening light photoperiods and decreasing temperatures as low as 0°C. Major N containing compounds and key enzymes in N metabolism during the senescence and low temperature acclimation were measured. The results showed that accessions of zoysiagrass from low latitudes had higher chlorophyll retention and lower N recycle (low soluble protein and NH4‐N) than those from higher latitudes in a simulated late fall, indicating that different mechanisms were responsible for the senescence and low temperature acclimation. In general, chlorophyll and soluble protein concentration decreased during the senescence which led to an increase of total free amino acids, especially glutamine, prior to the low temperature dormancy. The changes in N metabolism and the N uptake were also reflected in the decrease of NO3‐N and increase of NH4‐N, as well as relevant enzyme activities in the key steps. The signal and regulation mechanism in N cycling during the seasonal senescence and acclimation requires further investigations.

Annotation of Siberian Larch (Larix sibirica Ledeb.) Nuclear Genome-One of the Most Cold-Resistant Tree Species in the Only Deciduous GENUS in Pinaceae.

The recent release of the nuclear, chloroplast and mitochondrial genome assemblies of Siberian larch (Larix sibirica Ledeb.), one of the most cold-resistant tree species in the only deciduous genus of Pinaceae, with seasonal senescence and a rot-resistant valuable timber widely used in construction, greatly contributed to the development of genomic resources for the larch genus. Here, we present an extensive repeatome analysis and the first annotation of the draft nuclear Siberian larch genome assembly. About 66% of the larch genome consists of highly repetitive elements (REs), with the likely wave of retrotransposons insertions into the larch genome estimated to occur 4–5 MYA. In total, 39,370 gene models were predicted, with 87% of them having homology to the Arabidopsis-annotated proteins and 78% having at least one GO term assignment. The current state of the genome annotations allows for the exploration of the gymnosperm and angiosperm species for relative gene abundance in different functional categories. Comparative analysis of functional gene categories across different angiosperm and gymnosperm species finds that the Siberian larch genome has an overabundance of genes associated with programmed cell death (PCD), autophagy, stress hormone biosynthesis and regulatory pathways; genes that may play important roles in seasonal senescence and stress response to extreme cold in larch. Despite being incomplete, the draft assemblies and annotations of the conifer genomes are at a point of development where they now represent a valuable source for further genomic, genetic and population studies.

Comparative Genomics of Seasonal Senescence in Forest Trees.

In the course of evolution, both flowering plants and some gymnosperms have developed such an adaptation to winter and unfavorable living conditions as deciduousness. Of particular interest is Siberian larch (Larix sibirica Ledeb.), which is the only species in the pine family (Pinaceae) with a seasonal deciduousness. New generation sequencing technologies make it possible to study this phenomenon at the genomic level and to reveal the genetic mechanisms of leaf and needle aging in angiosperms and gymnosperms. Using a comparative analysis of the genomes of evergreen and deciduous trees, it was found that the genes that control EXORDIUM LIKE 2 (EXL2) and DORMANCY-ASSOCIATED PROTEIN 1 (DRM1) proteins are most represented in Siberian larch, while an excess of genes that control proteins acting as immune receptors were found in evergreens. Orthologs from the family of genes that control leucine-rich repeat receptor-like kinases (LRR-RLK) contributed mostly to the distinction between evergreens and deciduous plants.

No evidence for a negative effect of growing season photosynthesis on leaf senescence timing.

The length of the growing season has a large influence on the carbon, water, and energy fluxes of global terrestrial ecosystems. While there has been mounting evidence of an advanced start of the growing season mostly due to elevated spring air temperatures, the mechanisms that control the end of the growing season (EOS) in most ecosystems remain relatively less well understood. Recently, a strong lagged control of EOS by growing season photosynthesis has been proposed, suggesting that more productive growing seasons lead to an earlier EOS. However, this relationship has not been extensively tested with in-situ observations across a variety of ecosystems. Here, we use observations from 40 eddy-covariance flux tower sites in temperate and boreal ecosystems in the northern hemisphere with more than 10years of observations (594site-years), ground observations of phenology, satellite observations from the Moderate Resolution Imaging Spectroradiometer (MODIS), and three leaf senescence models to test the extent of a relationship between growing season photosynthesis and end of season senescence. The results suggest that there is no significant negative relationship between growing season photosynthesis and observed leaf senescence, flux-inferred EOS estimates, or remotely sensed phenological metrics, in most ecosystems. On the contrary, while we found negative effects of summer air temperatures and autumn vapor pressure deficit on EOS, more productive growing seasons were typically related to a later, not earlier, EOS. Our results challenge recent reports of carry-over effects of photosynthesis on EOS timing, and suggest those results may not hold over a large range of ecosystems.

PSXI-11 Assessing in situ rumen degradability of late season kudzu (Pueraria montana var. lobata)

Abstract Kudzu (Pueraria montana var. lobata) is an invasive weed species native to eastern Asia affecting much of the southeastern United States. Its broad leaves and viny growth allow it to outcompete native plant species for sunlight and nutrients. Kudzu, however, is a leguminous plant, making it a potential feedstock for ruminant species. Browsing ruminants in areas affected by kudzu could benefit animal productivity while serving to ameliorate rapid plant growth. This study specifically sought to assess the overall rumen degradability, rate of digestion, digestible fraction, and indigestible fraction using an in situ methodology. In situ rumen degradability was analyzed using four ruminally fistulated steers as individual experimental units over two repeated trials. Samples were incubated at 1, 12, 24, 36, 48, 60, and 72 h. Kudzu used in these trials was collected during September, making analysis in this study a reflection of degradability toward the end of the growing season. Data were analyzed as a randomized complete block design with repeated measures showing no significant differences between steers or trials (P > 0.05). Overall degradability across all steers and times was 69.79%. Significant changes in dry matter disappearance across all steers were observed at 1, 12, and 24 h (P < 0.05) with values of 33.86, 64.78, and 74.26%, respectively, and highest observed degradability at 72 h (79.55%). Incubation times between 24 and 72 h were not used in determining rate of digestion as dry matter disappearances throughout these times were not significantly different (P > 0.05). Rate of digestion (kd) was determined, using linear regression, to be 1.68% ∙ h-1 along with a digestible fraction (Do) of 28.29% and indigestible fraction (U) of 22.03%. The results of this study reflect that kudzu maintains a relatively high level of rumen degradability toward seasonal senescence, making it a functional feedstock even into the cooler months.

Effects of Soil Nitrogen Addition on Crown CO2 Exchange of Fraxinus mandshurica Rupr. Saplings

The impact of atmospheric nitrogen deposition on carbon exchange between forest and atmosphere is one of the research hotspots of global change ecology, past researchers have extensively studied the impacts on leaf level, while the impacts on crown CO2 exchange are still unclear. Therefore, we explored the impacts of different nitrogen addition levels on crown CO2 exchange of Fraxinus mandshurica saplings and their responses to the changes of major meteorological factors (photosynthetically active radiation, PAR; vapor pressure deficiency, VPD; and air temperature, Tair) with a novel automated chamber system. There are four levels of nitrogen addition treatments: control (no nitrogen addition, CK), 23 (low nitrogen addition, LN), 46 (medium nitrogen addition, MN), and 69 kgN·hm−2·a−1 (high nitrogen addition, HN). Our results showed that all nitrogen addition treatments increased daily average and accumulated gross primary production (GPP), crown respiration (R), and net crown CO2 exchange (Ne), especially at medium and high nitrogen levels. Similarly, maximum net photosynthetic rate (Nemax) and apparent quantum efficiency (α) were promoted. The change of Ne with PAR, Tair, and VPD showed that nitrogen addition postponed the appearance of photosynthesis midday depression. In addition, the monthly accumulation of R with all nitrogen addition treatments showed an increasing trend (June to July), and then decreased (July to September) during the growing season, while the Ne and GPP decreased gradually with seasonal vegetation senescence. Finally, the crown shifted from carbon sink to carbon source at the end of the growing season, however, the change under high nitrogen treatment occurred 3 days later. The crown CO2 exchange measurements provide a new perspective to better understand the response of forest ecosystem CO2 exchange to elevated nitrogen deposition and provide a basis for related carbon model parameter correction under the influence of nitrogen deposition.

Extensive transcriptome changes during seasonal leaf senescence in field-grown black cottonwood (Populus trichocarpa Nisqually-1)

To better understand the molecular control of leaf senescence, we examined transcriptome changes during seasonal leaf senescence in Populus trichocarpa Nisqually-1, the Populus reference genome, growing in its natural habitat. Using monthly (from May to October) transcriptomes for three years (2009, 2015, and 2016), we identified 17,974 differentially expressed genes (DEGs; false discovery rate <0.05; log-fold change cutoff = 0) from 36,007 expressed Populus gene models. A total of 14,415 DEGs were directly related to transitions between four major developmental phases – growth, senescence initiation, reorganization, and senescence termination. These DEGs were significantly (p < 0.05) enriched in 279 gene ontology (GO) terms, including those related to photosynthesis, metabolic process, catalytic activity, protein phosphorylation, kinase activity, pollination, and transport. Also, there were 881 differentially expressed transcription factor (TF) genes from 54 TF families, notably bHLH, MYB, ERF, MYB-related, NAC, and WRKY. We also examined 28 DEGs known as alternative splicing (AS) factors that regulate AS process, and found evidence for a reduced level of AS activity during leaf senescence. Furthermore, we were able to identify a number of promoter sequence motifs associated with leaf senescence. This work provides a comprehensive resource for identification of genes involved in seasonal leaf senescence in trees, and informs efforts to explore the conservation and divergence of molecular mechanisms underlying leaf senescence between annual and perennial species.

Seasonal senescence of leaves and roots of Populus trichocarpa-is the scenario the same or different?

The remobilization and resorption of plant nutrients is considered as a crucial aspect of the seasonal senescence of plant organs. In leaves, the mechanisms responsible for the relocation of valuable compounds are well understood while the related processes in roots are still being debated. Some research indicates that remobilization in roots occurs, while other studies have not found evidence of this process. Considering that the total biomass of fine roots is equal to or greater than that of leaves, clarifying the conflicting reports and ambiguities may provide critical information on the circulation of chemical elements in forest ecosystems. This study provides new information concerning the basis for remobilization processes in roots by combining physiological data with gene expression and protein levels. We suggest that, as in leaves, molecular mechanisms involved in nitrogen (N) resorption are also activated in senescent roots. An analysis of N concentration indicated that N levels decreased during the senescence of both organs. The decrease was associated with an increase in the expression of a glutamine synthetase (GS) gene and a concomitant elevation in the amount of GS—one of the most important enzymes in N metabolism. In addition, significant accumulation of carbohydrates was observed in fine roots, which may represent an adaptation to unfavorable weather conditions that would allow remobilization to occur rather than a rapid death in response to ground frost or cold. Our results provide new insights into the senescence of plant organs and clarify contentious topics related to the remobilization process in fine roots

Exploring Polarimetric Phase of Microwave Backscatter from Typha Wetlands

Despite their natural and societal importance, wetlands are becoming increasingly threatened. The goal of this study is to investigate the potential of polarimetric synthetic aperture radar (SAR) for monitoring one important vegetation constituent of wetlands: Typha. An idealized cylindrical scattering model is developed to portray double bounce microwave scattering from Typha stalks. Then a thin cylinder Brewster angle is introduced; relating the co-pol phase difference (CPD) of Typha to its relative permittivity. Full polarization Radarsat-2 data were acquired for a variety of dates and incidence angle ranges in order to validate the scattering model. The dependence of CPD on incidence angle is found to be consistent with the model. The evolution of CPD with seasonal senescence was also investigated. Despite some agreement with expected trends in vegetation moisture, there are anomalies that remain unexplained; possibly due to ecosystem change. This suggests the need for radar scatterometer experiments in a controlled environment to better explore the dependence of CPD on incidence angle and seasonal change. Last, cross-pol phase difference (XPD) histograms support other research in demonstrating the existence of coherent depolarization in Typha scattering. Additional modeling, entailing the detailed structure of the vegetation, will be necessary to understand this behavior.

Seasonal diet preferences of chital deer in the northern Queensland dry tropics, Australia

Chital deer (Axis axis) were introduced to the Burdekin dry tropics of north Queensland, Australia, in the late 1800s. Here rainfall and plant growth are highly seasonal and a nutritional bottleneck for grazing animals occurs annually before the wet season. This study describes the seasonal changes in diet and diet preference of chital in this seasonally-variable environment. Rumen samples were taken from 162 deer from two sites over the wet and dry seasons of two consecutive years and sorted macroscopically for identification. Relative seasonal availability of plant groups was estimated using step point sampling of areas grazed by chital. Chital alter their diet seasonally according to availability and plant phenology. Chital utilised 42 plant genera including grasses, forbs, subshrubs, shrubs, trees and litter. Grass consumption ranged from 53% of biomass intake during the dry season to 95% during the wet season. The predominance of grass in the wet season diet exceeded relative availability, indicating a strong preference. Although grass contributed more than half of the dry season diet it was the least preferred plant group, given availability, and the least actively growing. Shrubs were the preferred plant type in the dry season, and least subject to seasonal senescence. Composition and quantity of seasonal pastures vary markedly in north Queensland, and chital alter their diet by consuming those plants most actively growing. The increased dry season intake of non-grass forage appears to be a strategy to limit the detriment resulting from the progressive deterioration in the quality of grass.

Genome-Wide Characterization of AspATs in Populus: Gene Expression Variation and Enzyme Activities in Response to Nitrogen Perturbations

Aspartate aminotransferase (AspAT) catalyzes a reversible transamination reaction between glutamate and oxaloacetate to yield aspartate and 2-oxoglutarate, exerting a primary role in amino acid biosynthesis and homeostasis of nitrogen (N) and carbon metabolism within all cellular organisms. While progress in biochemical characterization of AspAT has been made for decades, the molecular and physiological characteristics of different members of the AspAT gene family remain poorly known particularly in forest trees. Here, extensive genome-wide survey of AspAT encoding genes was implemented in black cottonwood (Populus trichocarpa Torr. &amp; A. Gray), a model species of woody plants. Thorough inspection of the phylogenies, gene structures, chromosomal distribution, cis-elements, conserved motifs, and subcellular targeting resulted in the identification of 10 AspAT isogenes (PtAspAT1-10) in the Populus genome. RNA-seq along with quantitative real-time polymerase chain reaction (qRT-PCR) validation revealed that PtAspATs displayed diverse patterns of tissue-specific expression. Spatiotemporal expressions of homologous AspATs in the poplar hybrid clone ‘Nanlin895’ were further evaluated, showing that gene expressions varied depending on source-sink dynamics. The impact on AspAT transcripts upon N starvation and seasonal senescence showed the upregulation of five AspAT in leaves concurrent with drastic downregulation of six or more AspATs in roots. Additionally, marked reductions of many more AspATs transcripts were observed in roots upon N excess. Accordingly, AspAT activities were significantly suppressed upon N starvation by an in-gel assay, prompting the argument that enzyme activity was a more direct indicator of the growth morphology under a N stress regime. Taken together, the expression profiling and enzyme activities upon stress cues provide a theoretical basis for unraveling the physiological significance of specific gene(s) in regulation of N acquisition and remobilization in woody plants.

The relationship between physiological and mechanical properties of Acer platanoides L. and Tilia cordata Mill. leaves and their seasonal senescence

The seasonal senescence of leaves in the phenological cycle coincides with the change of their strength properties which determine resistance to environmental conditions and the efficiency of the photosynthesis process. That affects the development, growth and condition of the plant. Therefore, the aim of this paper was to observe and compare the results of strength tests performed on the leaves of two species of trees popular in Poland - lime and maple. As well as chlorophyll fluorescence and photosynthetic pigments content in the context of the changes occurring during the entire leaf life cycle. Obtained results showed that the strength properties of the tested leaves reached the minimum values in spring and the maximum in the summer similarly to the leaf greenness index. Whereas the fluorescence increased which the seasonal senescence in opposition to the photosynthesis efficiency of the leaves. Collected data revealed that strength parameters and photosynthetic pigment content were significantly higher for maple leaves than for lime leaves. Studies showed differences between physiological and mechanical properties of the leaves of two trees species, even if they grew under the same environmental conditions. It is concluded from the results that phenotype and physical parameters of leaves are related to seasonal senescence.

Autophagy counteracts instantaneous cell death during seasonal senescence of the fine roots and leaves in Populus trichocarpa

BackgroundSenescence, despite its destructive character, is a process that is precisely-regulated. The control of senescence is required to achieve remobilization of resources, a principle aspect of senescence. Remobilization allows plants to recapture valuable resources that would otherwise be lost to the environment with the senescing organ. Autophagy is one of the critical processes that is switched on during senescence. This evolutionarily conserved process plays dual, antagonistic roles. On the one hand, it counteracts instantaneous cell death and allows the process of remobilization to be set in motion, while on the other hand, it participates in the degradation of cellular components. Autophagy has been demonstrated to occur in many plant species during the senescence of leaves and flower petals. Little is known, however, about the senescence process in other ephemeral organs, such as fine roots, whose lifespan is also relatively short. We hypothesized that, like the case of seasonal leaf senescence, autophagy also plays a role in the senescence of fine roots, and that both processes are synchronized in their timing.ResultsWe evaluated which morphological and cytological symptoms are universal or unique in the senescence of fine roots and leaves. The results of our study confirmed that autophagy plays a key role in the senescence of fine roots, and is associated also with the process of cellular components degradation. In both organs, structures related to autophagy were observed, such as autophagic bodies and autophagosomes. The role of autophagy in the senescence of these plant organs was further confirmed by an analysis of ATG gene expression and protein detection.ConclusionsThe present study is the first one to examine molecular mechanisms associated with the senescence of fine roots, and provide evidence that can be used to determine whether senescence of fine roots can be treated as another example of developmentally programmed cell death (dPCD). Our results indicate that there is a strong similarity between the senescence of fine roots and other ephemeral organs, suggesting that this process occurs by the same autophagy-related mechanisms in all plant ephemeral organs.

Quantifying mortality of modular organisms: a comparison of partial and whole‐colony mortality in a colonial bryozoan

AbstractComprehensive studies on the population dynamics of colonial organisms require estimates of mortality from the level of the individual module (partial mortality) to the entire colony (whole‐colony mortality), as well as determining the factors that affect mortality at each level of organization. However, accurate measurements of whole‐colony and partial mortality of modular organisms can be difficult to obtain, and few studies involve concurrent measurements of modular and colony mortality. We implemented multiple approaches to measure whole‐colony and partial mortality of modular species using the colonial bryozoan Membranipora membranacea as a model organism. M. membranacea is a cosmopolitan species and of particular ecological significance in the northwest Atlantic, where it is the dominant epiphyte on laminarian kelps and the main driver for the defoliation of kelp beds. Rates of whole‐colony mortality were measured in the field (1) indirectly through repeated subsampling cohort analyses and (2) directly by tagging colonies in situ. Partial mortality of colonies was measured (1) in the field by quantifying the proportion of degenerated zooids per colony and (2) in the laboratory by monitoring loss of colony surface area during seasonal senescence of colonies over winter. Temporal patterns differed substantially between partial and whole‐colony mortality, suggesting that factors affecting mortality of individual modules differ from those affecting whole colonies. Our study indicated that the accuracy of common methods for measuring mortality of modular organisms depends on the species‐specific life‐history characteristics. However, we suggest that rates and mechanisms of whole‐colony and partial mortality can be most accurately quantified by revisiting tagged individuals in situ and recording loss of entire colonies (whole‐colony mortality) and loss of living colony area (partial mortality) between successive sampling times. For M. membranacea, measuring whole‐colony or partial mortality in isolation would overestimate important demographic rates and underestimate the influence of temperature on mortality, respectively. This study demonstrates the need to include mortality measurements from the level of the individual module to the entire colony when quantifying population dynamics of colonial organisms.

Photochemical and antioxidative responses of the glume and flag leaf to seasonal senescence in wheat.

The non-leaf photosynthetic organs have recently attracted much attention for the breeding and screening of varieties of cereal crops to achieve a high grain yield. However, the glume photosynthetic characteristics and responses to high temperature at the late stages of grain filling are not well known in winter wheat (Triticum aestivum L.). In the present study, an experiment was conducted to investigate the anatomy, chloroplast temporal changes, chlorophyll fluorescence, xanthophyll cycle and antioxidative defense system in glumes of field-grown wheat during grain filling compared with flag leaves. Observations using a light microscope revealed that the glumes developed a solid structural base for performing photosynthesis. Compared with the flag leaves, the glumes preserved a more integral ultrastructure, as observed under transmission electron microscopy, and had higher values of Fv/Fm and ΦPSII at the maturity stage. Further analysis of the chlorophyll fluorescence demonstrated that the glumes experienced high non-photochemical quenching (NPQ) at the late stages. Determination of the pool size of the xanthophyll cycle suggested that the (A+Z)/(V+A+Z) ratio was consistently higher in glumes than in flag leaves and that the V+A+Z content was considerably higher in glumes at the maturity stage. In addition, the glumes exhibited a higher antioxidant enzyme activity and a lower accumulation of reactive oxygen species. These results suggest that the glumes are photosynthetically active and senesce later than the flag leaves; the advantages may have been achieved by coordinated contributions of the structural features, higher NPQ levels, greater de-epoxidation of the xanthophyll cycle components and antioxidative defense metabolism.

Invasive plant ecosystem engineer facilitates community and trophic level alteration for brackish marsh invertebrates

Wetland habitats are becoming increasingly scarce worldwide while experiencing exceptionally high levels of plant invasion. Invasive plant species affect ecosystems through numerous avenues, including acting as ecosystem engineers, contributing unique plant functional traits, and altering trophic dynamics. We examined the impacts of the invasive weed Lepidium latifolium on soil‐dwelling and canopy‐dwelling invertebrate communities in a brackish marsh of northern California. Invertebrate abundance, species richness, diversity, and community composition were measured in both invaded and non‐invaded areas of the marsh in four time periods that correspond with different L. latifolium phenologic phases. We observed these different stages of L. latifolium, and we recorded alterations in the habitat structure provided by L. latifolium as the plant entered senescence. L. latifolium had differential impacts on the two invertebrate communities studied. The presence of L. latifolium increased abundance of soil‐dwelling invertebrates and decreased the species richness of canopy‐dwelling invertebrates in the tidal marsh‐terrestrial ecotone where L. latifolium ameliorates harsh physical conditions. No changes occurred in the tidal marsh plain and fringing tidal marsh where L. latifolium's physical structure is more similar to existing non‐L. latifolium structure. During full senescence, L. latifolium reduced canopy‐dwelling invertebrate abundance and altered trophic dynamics, shifting composition to a predator‐dominated community. Our results demonstrate that L. latifolium can impact brackish marsh ecosystems, and we hypothesize L. latifolium's unique functional traits (large inflorescence and seasonal senescence) enable it to act as an ecosystem engineer that alters community and trophic dynamics. Mechanisms through which plant invaders alter ecosystem processes are dynamic in space and time, thus, future research will require the use of manipulative experiments to inform site‐specific management strategies.

Inoculation of growing media with the rhizobacterium Variovorax paradoxus 5C-2 reduces unwanted stress responses in hardy ornamental species

Inoculation of growing media with plant growth promoting rhizobacteria (PGPR) has a number of potential benefits for the production of ornamental plants. Certain rhizobacteria synthesise the enzyme ACC deaminase, which cleaves ACC, the precursor of the plant hormone ethylene. Bacterial metabolism is now known to lead to a reduction in [ACC] in the plant transpiration stream and bacteria are hypothesised to act on ACC exuded from roots. This in turn reduces the ethylene generated in plants growing in growing media inoculated with these bacteria. Here we tested if applications of the ACC deaminase containing rhizobacteria Variovorax paradoxus 5C– 2could be of benefit to ornamental growers by reducing ethylene generation in stressed plants. Ethylene is produced at levels that are inhibitory to growth and development under a number of abiotic stresses. The propagation and production of hardy ornamentals is deleteriously affected by abiotic stresses that involve ethylene signalling, including drought and physical wounding. Inoculation of growing media with V. paradoxus 5C-2 lowered ethylene emission from mature leaves of Cytisus × praecox experiencing drought stress. In addition, bacterial inoculation of the growing media resulted in significantly reduced abscission of the mature leaves under drought treatment. Beneficial effects of inoculation where also found in the wounding response of Fargesia murielae following divisional propagation and late season senescence in Aquilegia × hybrida in response to drought stress. Together these results demonstrate that V. paradoxus 5C– 2has real potential for use on ornamental nurseries in situations where plant stresses are unavoidable.

Is iron phloem mobile during senescence in trees? A reinvestigation of Rissmüller’s finding of 1874

As long as 130 years ago Rissmüller reported substantial retranslocation of iron (Fe) from beech leaves (Fagus sylvatica L.) shortly before leaf fall. This rather limited report on Fe retranslocation via the phloem in plants was the reason for this research to study changes in Fe content in individual beech leaves in more detail during the vegetative period. Besides Fe, other micronutrients and particularly Ca and K, well known to differ substantially in phloem mobility, were analysed as mineral nutrient markers. In addition to beech, other deciduous and evergreen species of Angiosperms and Gymnosperms were also studied. As expected, there was no evidence of Ca retranslocation from senescent leaves, while K as a phloem mobile mineral nutrient was retranslocated in fall in deciduous but not in evergreen trees. There was no indication to support Rissmüller's finding of Fe retranslocation in any of the different species studied. From these results, we conclude that natural leaf senescence of trees during late season does not induce retranslocation of Fe and other micronutrients. Possible reasons for the absence of a distinct retranslocation of Fe in the species studied during late season senescence are the lack of a sink activity, as for example the development of seeds in annual plant species (e.g., cereals), or the presence of a root system still active enough to provide Fe and other mineral nutrients for plant demand, and both factors have to be considered in further studies. Reviewing the data in the literature on Fe and Zn retranslocation during senescence, we conclude that in principle both micronutrients are potentially phloem mobile. However, various prerequisites are needed for the occurrence of phloem mobility which were absent in the plant species studied. Regardless of this conclusion, we recommend that in general early published research data need a critical re-evaluation.