Our aim here was to assess the seasonal (dry, ebb, and rainy seasons), spatial (upstream, intermediate, and downstream), and environmental effects on the dynamics of Gerridae assemblages (Heteroptera: Gerromorpha) in a Cerrado stream, in central-western Brazil. We sampled the insects on the water line between May 2011 and April 2014 with an 18 cm diameter sieve. We used the scanning method in 100 m of stream in each sampled locality. We sampled 3690 individuals of 19 species. There was a seasonal difference in abundance, which was a result of a lower abundance in the rainy season, but this did not differ between different environments. The estimated species richness was lower upstream and in the rainy season. Species composition was different between the upstream and downstream portions. It was also different among all seasons, with the greatest difference occurring between the rainy and dry seasons. The abiotic factors were responsible for structuring the assemblages in different seasons. The observed differences among seasons in abundance, richness, and species composition have implications for conservation since changes in the structure of the vegetation in the stream edges change the hydrological cycle of streams, and consequently the diversity of the Gerridae assemblages. There was variation in richness and composition between such small distances (upstream and downstream). This shows how sensitive this system can be, and how important elements of the stream's trophic, with the Gerromorpha, can be easily altered.