Scientific Resources Research Articles

Saccharomyces Genome Database: Advances in Genome Annotation, Expanded Biochemical Pathways, and Other Key Enhancements

Abstract Budding yeast (Saccharomyces cerevisiae) is the most extensively characterized eukaryotic model organism and has long been used to gain insight into the fundamentals of genetics, cellular biology, and the functions of specific genes and proteins. The Saccharomyces Genome Database (SGD) is a scientific resource that provides information about the genome and biology of S. cerevisiae. For more than 30 years, SGD has maintained the genetic nomenclature, chromosome maps, and functional annotation for budding yeast along with search and analysis tools to explore these data. Here we describe recent updates at SGD, including the two most recent reference genome annotation updates, expanded biochemical pathways representation, changes to SGD search and data files, and other enhancements to the SGD website and user interface. These activities are part of our continuing effort to promote insights gained from yeast to enable the discovery of functional relationships between sequence and gene products in fungi and higher eukaryotes.

Supporting Evidence in Phase 2 Cancer Trial Protocols: A Content Analysis

Abstract Background Phase 2 trials are instrumental for designing definitive efficacy trials or attaining accelerated approval. However, high attrition of drug candidates in phase 2 raises questions about their supporting evidence. Methods We developed a typology of supporting evidence for phase 2 cancer trials. We also devised a scheme for capturing elements that enable an assessment of the strength of such evidence. Using this framework, we content analyzed supporting evidence provided in protocols of 50 randomly sampled phase 2 cancer monotherapy trials starting between January 2014 and January 2019, available on ClinicalTrials.gov. Results Of the 50 protocols in our sample, 52% were industry funded. Most invoked supporting evidence deriving from trials against different cancers (n = 28, 56%) or preclinical studies (n = 48, 96%), but not from clinical studies involving the target drug-indication pairing (n = 23, 46%). When presenting evidence from models, only one protocol (2%) explained their translational relevance. Instead, protocols implied translatability by describing molecular (86%) and pathophysiological (84%) processes shared by model and target systems. Protocols often provided information for assessing the magnitude, precision and risk of bias for supporting trials (n = 43, 93%, 91%, 47%, respectively). However, such information was often unavailable for preclinical studies (n = 49, 53%, 22%, 59%). Conclusion Supporting evidence is key to justifying the commitment of scientific resources and patients to a clinical hypothesis. Protocols often omit elements that would enable critical assessment of supporting evidence for phase 2 monotherapy cancer trials. These gaps suggest the promise of more structured approaches for presenting supporting evidence.

DIAGNOSTIC APPROACHES TO PAINFUL TEMPOROMANDIBULAR JOINT DYSFUNCTION: A LITERATURE REVIEW

Introduction. The temporomandibular joint (TMJ) is an anatomically and biomechanically complex structure, essential for critical functions such as chewing, breathing, verbal and non-verbal communication, and facial expressions. The term “temporomandibular joint disorders” encompasses a range of conditions characterized by pain with diverse, often polymorphic, manifestations. Purpose of this study is to analyze the trends and directions in publications on the diagnosis of painful temporomandibular joint disorders from 2017 to 2024 (up to March 31, 2024) across international and Ukrainian scientific resources. Materials and methods. We used the bibliosemantic method, the methods of the system approach and content analysis. We searched for scientific publications for the period from 2017 to 2024 as of 03/31/2024 in Google Academy and Medline (accessed via PubMed) using the following words and their combinations: “dysfunctional temporomandibular joint conditions” and “temporomandibular joint dysfunction”, “temporomandibular dysfunction and condylare position”, “temporomandibular dysfunction and classification”. Inclusion criteria were as follows: a) studies aimed at evaluating the anatomy of the temporomandibular joint; b) studies conducted with cone beam computed tomography images; c) research on humans; d) data of persons aged 18 to 60 years. Exclusion criteria were as follows: a) clinical cases; b) discussions; c) research with the participation of children and adolescents (under the age of 18). Results. For the period 2017-2024 (as of 31.03.2024), the main research topics on the painful form of temporomandibular joint dysfunction are the following: the prevalence of temporomandibular joint disorders (11 publications), the influence factors of the dysfunction development of the temporomandibular joint (42 publications), the improvement of existing and the search for new diagnostic criteria (17 publications); the anatomical and the morphological features of the temporomandibular join as the development predictors for the temporomandibular joint dysfunction (20 publications); search for new models for predicting the course and effectiveness of treatment for temporomandibular join dysfunction (4 publications). Conclusion. Researchers have primarily focused on examining factors influencing the clinical progression of temporomandibular joint dysfunction, accounting for 44.7% of all publications. Studies on anatomical and morphological features as predictors of temporomandibular joint dysfunction, along with new predictive models, remain contentious and highlight the need for further investigation.

Strength, Durability, and Aesthetics of Corner Joints and Edge Banding in Furniture Design: A Review

Corner joints and edge banding are essential components that significantly impact the strength, durability, and aesthetic appeal of particleboard furniture. This review examines the critical role of edge banding in enhancing the performance of corner joints, which are fundamental to the overall quality of panel furniture. A targeted literature search was conducted across key databases, including the Web of Science Core Collection (WoS CC), Scopus, and Google Scholar, focusing on scientific resources in the technical and biotechnical sciences. The selection of joint types, materials, and construction methods can substantially influence both the structural integrity and visual design of the furniture. Well-designed corner joints improve durability and longevity by ensuring that furniture can withstand various forces and loads without failure or deformation. These joints enhance the aesthetics of furniture by providing seamless and visually appealing connections between different elements. Edge banding is vital for reinforcing corner joint strength, as different materials exhibit varying degrees of resistance to impact, scratches, and abrasion, thereby safeguarding furniture surfaces. Also, edge banding contributes to the furniture’s longevity, ensuring durability during use as well as through disassembly and transport during remodeling or relocation. This review aims to consolidate existing knowledge and establish parameters for future research on the quality and performance of corner joints and edge bands in particleboard furniture.

Effect of heatwaves on mortality of Alzheimer's disease and other dementias among elderly aged 60 years and above in China, 2013–2020: a population-based study

BackgroundChina has the largest number of dementia patients in the world, posing a significant health and economic burden. Alzheimer's disease (AD) and other dementia patients face a higher risk of mortality during heatwaves, but relevant studies on this topic have been limited so far. MethodsThe study extracted data from the China Cause of Death Reporting System (CDRS) on deaths of AD and other dementia patients aged 60 years and above between 2013-2020. Using an individual-level, time-stratified, and case-crossover study design, the effects of heatwaves across nine scenarios on dementia mortality were quantified by conditional logistic regression combined with distributed lag non-linear model (DLNM). Additionally, the attributable fractions (AFs) of deaths due to heatwaves were calculated. FindingsA total of 399,036 death cases were reported caused by AD and other dementias during the study period. It was found that heatwaves significantly increased the risk of death among people with AD and other dementias. As the intensities and durations of the heatwaves increased, the lag0-7 cumulative odds ratios (CORs) of mortality increased progressively from 1·140 (95% CI: 1·118, 1·163) under the mildest heatwave to 1·459 (95% CI: 1·403, 1·518) under the most severe one, across nine heatwave scenarios examined. Additionally, under specific heatwave scenarios, sex and regions modified the mortality risk, but no significant age differences were observed. The AFs of AD and other dementia mortality due to milder heatwaves were lower compared to more severe heatwaves, ranging from 12·281% (95% CI: 10·555%, 14·015%) to 31·460% (95% CI: 28·724%, 34·124%). InterpretationThe study provided critical insights into the substantial increase in heatwave-related mortality among AD and other dementia patients during and after heatwave events. The results from our quantitative analyses will provide needed scientific evidence for policymakers and practitioners to develop relevant policies and guidelines to protect the health and well-beings of vulnerable populations in future in the context of both seasonal changes and long-term climate change. FundingThis work was supported by the Project of Prevention and Intervention on Major Diseases for Elderly in China, NCNCD [00240201307], the National Key Research and Development Program of China [2022YFC2602301, 2023YFC2308703] and the Science and Technology Fundamental Resources Investigation Program of China [2017FY101201].

Medical Science Liaisons: A Guide for Advanced Practice Registered Nurses

Medical science liaisons (MSLs) are part of the field medical affairs (FMA) department of pharmaceutical companies and are scientific resources for advanced practice registered nurses (APRNs). Following strict regulations and antikickback statutes, MSLs operate differently from sales and engage in unbiased, nonpromotional scientific exchange to provide clinical resources to APRNs. They also collaborate with shareholders like professional organizations, clinical trial sites, and academic institutions. Understanding MSL engagement allows APRNs multiple avenues both externally and within the pharmaceutical industry to leverage their experience to enhance clinical practice.

Corrigendum to ‘RadLab: An open science resource for radiation studies relevant to human spaceflight’ Life Sciences in Space Research Volume 43, November 2024, Pages 29-34

Corrigendum to ‘RadLab: An open science resource for radiation studies relevant to human spaceflight’ Life Sciences in Space Research Volume 43, November 2024, Pages 29-34

The Utilisation of ICT Resources for Teaching and Learning Science in Rural Schools: A Bibliometric Analysis

Information and Communication Technology (ICT) offers pedagogical approaches in science that may enhance engagement in STEM disciplines. It has emerged as a catalyst for quality education and job creation, imparting scientific knowledge and potentially enhancing educational standards for rural students, hence generating increased opportunities for employment and entrepreneurship. This study aimed to explore research trends on the use of ICT in teaching and learning science in rural schools, using available big data on studies done in the past 10 years worldwide. A bibliometric analysis was used to analyse 171 journal articles and conference proceedings papers which were published or in press. These documents were obtained through the SCOPUS database. The keywords used were "ICT in teaching science” OR "rural schools". Data were organised into tables, graphs and visualisations obtained from VOSviewer, then analysed descriptively. Findings from the study revealed insufficient publications of documents in this area per year worldwide, citations and co-citations, poor collaborations and low total link strength for all documents published from 2013-2023. Whereas many studies have been conducted on the use of ICT or STEM education or rural education, few studies incorporated the three terms. Those who have embarked on these studies seem to be losing interest in doing more research in this area. Furthermore, there are insufficient collaborations between authors and countries in this research area. This study recommends that the amount of research and collaborations be increased on regarding the utilisation of ICT resources for teaching science in rural schools.

Benefits of cross-border access to human genomes at scale for research and healthcare

Abstract Genomics data will soon be routinely generated and integrated into national healthcare systems. To maximise the potential of genomic medicine, data should be accessible for research where possible. This is the remit of ELIXIR, an intergovernmental organisation that brings together life science resources from across Europe. Innovative solutions are needed to ensure the validated research findings for disease or preventative medicine are then integrated into healthcare. To tackle this, the 1+MG initiative, a joint initiative of 25 EU countries, the UK, and Norway, aims to enable secure access to genomics and the corresponding clinical data across Europe for better research, personalised healthcare and health policy making. In the design and scale-up phase (B1MG project), recommendations and guidelines to advance towards the deployment of personalised medicine at a European scale have been produced, adopted by 1+MG and developed into a 1+MG framework. This includes guidance on data governance, standards, quality and infrastructure, recommendations on how to approach citizen engagement and a tool for countries to self-assess implementation into healthcare. The European Genomic Data Infrastructure (GDI) project supports the scale-up and sustainability phase of the 1+MG initiative, to deploy infrastructure across 24 countries to support the overall ambition. Recommendations are being used to promote governance and technical interoperability across European initiatives including the European Health Data Space (EHDS), and European Cancer Image Initiative (EUCAIM). The 1+MG will be established as a European Data Infrastructure Consortia in 2025 and will act as an Authorised Participant in the EHDS providing access to a permanent high-quality federated data collection of genomic and health data that will accelerate research, innovation and policymaking facilitating the deployment of genomic medicine across Europe.

Citizen Science and Multimedia Didactic Resources: Knowledge of Mosquito-Borne Diseases in an Urban Area of Southwestern Colombia

Citizen science resources have had great relevance in community educational intervention, fostering interest in ecological research projects. This study investigated the influence of the application of multimedia didactic resources focused on citizen science and analysis of sociodemographic variables to improve the levels of ecological knowledge about mosquito-borne diseases. For this, a probabilistic sample of 172 participants from an urban sector in southwestern Colombia was selected. A multimedia didactic material was designed for the educational intervention and evaluated by means of pretest and posttest. To assess whether the educational intervention was statistically significant, the data were processed with statistical inference to determine the influence of various variables (gender, age, marital status, schooling, and occupation) on each person’s ecological knowledge. The findings showed a significant increase in the knowledge levels of the participants. The marital status variable (married and cohabiting) significantly influenced ecological knowledge. These participants are more likely to obtain high knowledge, associated with the prevention of their family environment. This study demonstrated that multimedia didactic strategies are an important factor to take into account in the structuring of environmental education and citizen science projects. For future research, it is suggested to deepen the relationship between sociodemographic variables and ecological knowledge.

Studying technical sciences without borders: We use the navigator for open educational resources. Part 1. The Russian experience

Introduction. The introduction of information and communication technologies into learning processes expands the possibilities of using information resources and becomes one of the priorities of the transforming higher education system. These changes are reflected in the trends in the development and application of open educational resources. However, the scale and fragmentation of data on the Internet, as well as the lack of open educational resources standards, makes the information retrieval process a difficult task and requires professional skills.Purpose setting. To determine the relevance and prospects for the development of sectoral open educational resources, it is necessary to analyze their role in the modern educational system. It is important to identify the possibilities of using open access research in technical sciences as one of the tools to eliminate social inequality in higher education. To identify and present arguments for the purpose of justifying the need to develop an industry-specific «Navigator on OER of Technical Sciences» (hereinafter referred to as the «Navigator»).Methodology and methods of the study. The research methodology covers the study and analysis of scientific papers devoted to the development of tools and initiatives in the field of open educational resources in technical sciences both in Russia and abroad. This study is also based on the method of oral questioning of students, postgraduates and teachers of technical disciplines at universities in Novosibirsk. Results. An array of scientific literature and web resources has been studied, providing free access to scientific and educational information in the field of research under consideration. Taking into account empirical data, the necessity of developing a «Navigator» on open educational resources in the system of higher technical education is justified (http://lib-os.ru/issledovatelyam/resursy/obrazovatelnye- resursy/navigator-oor-techn-nauk/).Conclusion. The study of technical sciences using open educational resources contributes to the effective improvement of professional level and allows you to be aware of current solutions to innovative activities in the field of knowledge under consideration.

Land Use Change in a Typical Transect in Northern China and Its Impact on the Ecological Environment

This paper uses seven periods of land use/cover data from 1990 to 2020 to investigate the spatio-temporal features of land use change in a typical transect in northern China. By using the land use transfer matrix, transect analysis, and ecological contribution rate, image interpretation data obtained from the Chinese Academy of Sciences Resource and Environmental Science Data Platform were analyzed using a spatial overlay to quantitatively examine the transect’s land use changes and their impact on the eco-environment. The results indicate that (1) the transect’s land use is dominated by cropland and grassland. (2) Cropland, forest land, and waters experienced significant transitions in 1995 and 2000, which was linked to socio-economic development and policy factors. (3) The total dynamic percentage of land use change is 4.52%, with built-up land and cropland showing the highest change. (4) The transect’s eco-environmental quality (EQ) has significantly declined, with the quality index dropping from 0.3839 to 0.3773. The transformation of cropland to forest land improves the eco-environment, while the transformation to built-up land has negative impacts. Human activities adversely affect the EQ. The findings are promising for leading the development of conserving the eco-environment and supporting the formation of regionally differentiated paths of ecological civilization construction in the transect.

Bullying Prevention at Schools in Ukraine

The purpose of the article is to inform high school students and teachers about the problem of preventing bullying and implementing anti-bullying measures at schools in Ukraine. Methodology. Theoretical research methods were used in the article: systematization; comparison of issues related to bullying prevention; comparison of different author’ views regarding the implementation of anti-bullying measures; and analysis and generalization of scientific, educational, methodological, psychological, and pedagogical resources with the aim of systematizing approaches to bullying prevention. Results. In order to deepen high school students’ knowledge of anti-bullying skills, we developed and offered two lessons for students. They are “Normative and legal documents on the prevention of bullying and responsibility for it”, “Foreign experience in preventing bullying”, as well as two workshops for teachers, they are “Guidance to teachers on the implementation of anti-bullying measures in Ukraine”, “Tips for teachers on bullying prevention according to the national programs”‎. We believe they will contribute to bullying prevention in Ukraine. Conclusions. Conclusions regarding the effective bullying prevention in Ukrainian schools indicate the need for comprehensive, systematic work. The most effective are programs that cover all aspects of school life from creating a friendly and supportive environment to raising awareness among students, teachers, and parents about the bullying problem. It is important to involve students in the process of bullying prevention through their participation, communication, and creation of student councils that help in conflict resolution. Building interaction and empathy skills among students helps to reduce the level of aggression and bullying. Systematic training for teachers about bullying prevention, as well as the inclusion of bullying topics in school programs, contribute to the development of a safe educational environment in schools. In order to prevent bullying effectively, it is important that students collaborate actively with teachers, parents, and local communities, ensuring that bullying is prevented and countered both inside and outside schools.

Construction of Virtual Experiment Teaching Platform based on 5E Teaching Mode and VI Technology

Virtual instruments have many advantages such as low cost, comprehensive functionality, easy scalability, and easy replication. At the same time, they can also meet the special requirements of time and space for remote experiments. Based on Web technology and virtual instrument technology, a virtual experiment system is constructed, which combines data sharing, remote control, and real-time operation. More conducive to utilizing experimental and scientific research resources. At the same time, virtual instruments have high flexibility and are no longer limited by traditional experimental teaching modes, which can save a lot of repeated investment costs. They can also keep the teaching equipment in the laboratory up-to-date and closely follow the development trend of science and technology. As a compulsory course for mechanical engineering majors, although the existing experimental system has achieved certain results, there are still limitations to 'Mechanical Engineering Testing Technology'. This article aims to address the problems of insufficient instrument sets and outdated equipment in the experimental teaching of "Mechanical Engineering Testing Technology", as well as students' poor ability to apply theory to practice and insufficient innovation ability. By integrating the 5E teaching mode, a virtual experimental teaching platform based on VI (Virtual Instrument) technology is established. Students can break through traditional experimental methods and conduct various experiments through the network, obtaining experimental experiences that are very similar to real experiments. At the same time, it can deepen their understanding and recognition of experiments, and better grasp theoretical knowledge.

CONTROVERSIAL ANALYSIS OF CANNABIS RECLASSIFICATION: PERSPECTIVES IN A NARRATIVE REVIEW

Cannabis has a long and controversial tradition. A narrative review was conducted in 2024 with the aim of analyzing the discrepancies in the reclassification of cannabis for medical use and research in 2020. Indexed journals, scientific digital resources, reports from international organizations, States and expert opinions from institutions and scientists were critically examined. The results showed scientific evidence on risks and possible benefits, with a complex, biased, contradictory and inconclusive history. The damage to health and life led to its classification in an international regulatory legal framework, violated by drug trafficking and the patenting of molecules that do not meet the requirements as drugs, trivializing their abuse. After legalization, abuse, trafficking and harm increased, especially among young people. The pro-legalization environment of high-potency cannabis in a globalized world has decreased the perception of risk, even among health personnel, distorting communication. The thriving cannabis industry, with its sophisticated marketing, attracts young people. It was concluded that methodological and practical inconsistencies persist in research, weakening the international drug control regime. The benefits of reclassification are not evident; on the contrary, abuse, trafficking, physical and mental illness, indirect deaths and damage to the environment continue to increase, which could lead to the legalization of all drugs, threatening human health and survival.

Innovative Service Model of Science and Technology Finance in China

This investigation analyses the financial dependence of the transformation of scientific and technological achievements, the effective connection between scientific and technological resources and financial resources, the development of emerging industries, the upgrade of technological levels and the optimization of industrial structures, and the financial support required for technological innovation and R&D of high-tech enterprises as well as small and medium-sized technological enterprises. It is pointed out that the improvement of financial products, the innovation of capital markets and the application of internet finance are the operational basis of the innovation service mode of science and technology finance. Correspondingly, the upper level design at the policy level provides the basis for the development of the appropriate service model of technological and financial innovation.

Silicon-based quantum random number generator with untrusted sources and uncharacterized measurements

Random numbers are essential resources in science and engineering, with indispensable applications in simulation, cybersecurity, and finance. Quantum random number generators (QRNGs), based on the principles of quantum mechanics, ensure genuine randomness and unpredictability. Silicon photonics enables the large-scale deployment of integrated QRNGs due to its low cost, miniaturization, and compatibility with CMOS technology. However, current integrated QRNGs are typically based on perfect or partially perfect device models, deviating from real-world devices, which compromises the unpredictability of quantum random numbers. In this study, we implemented a silicon-based QRNG that makes no assumptions about the source and only uses trusted but uncharacterized measurement devices. In experimental demonstration, we show that our setup can generate secure random numbers with different choices of intensities of laser light, and achieve an optimized random number generation rate of up to 4.04 Mbps. Our work significantly advances the security, practicality, and commercial development of QRNGs by employing imperfect devices.

Developing a green economy towards sustainability: Research in Vietnam in the context of digital transformation

In recent years, the Party and State have paid great attention to promoting green economy, circular economy and sustainable development. Thereby, many policies have been issued to orient economic development towards sustainability, environmental protection, climate change adaptation, improving investment quality and efficiency, and focusing on attracting high-quality projects. However, in reality in Vietnam, the trend of green economic development is only at the starting point, the green economic development process is still lacking in synchronization and facing barriers in terms of capital, human resources, science and technology resources... In the face of environmental and social consequences, from developing the brown economy, countries have gradually shifted to a green economy - an economy that cares about happiness, social justice and the environment in addition to economic goals. Vietnam is no exception to this trend. Green economic development has been of interest to the Party and the State since the early years of the Doi Moi period and has achieved certain successes. However, the construction of a green economy in Vietnam still faces many limitations due to many reasons, such as: lack of capital resources, labor quality, science and technology level not meeting requirements... This poses synchronous and long-term solutions with each specific step towards a green economy. The article reflects the current situation and solutions for green economic development in Vietnam. Keywords: Sustainability, Green Economy, Digital Transformation.

Territorial Patterns of European Innovation in the Context of Different Innovation Output Proxies: A Spatial MGWR-SAR Approach

AbstractThe paper seeks to explore the drivers of European innovation represented by three innovation outputs (patent, trademark, and design applications), emphasizing spatial autocorrelation and heterogeneity. It includes data from 202 regions from 22 European Union (EU) Member States, along with 18 regions from Switzerland, Norway, and Serbia in 2019, providing a more comprehensive geographic scope. By considering multiple indicators of innovation output, including patents, trademarks, and design applications, the main objective is to examine spatial innovation spillovers and the heterogeneous responses of regional innovation output to innovation inputs in the context of European regions. To achieve this goal, the main instrument of the analysis is a newly proposed methodological framework called Mixed Geographically Weighted Regression-Spatial Autoregressive (MGWR-SAR) models. The analysis suggests that while all innovation inputs (most-cited publications, research and development expenditure in the business sector, human resources in science and technology, and population density) are justified in increasing all innovation outputs, the strength of particular determinants of innovation might vary across regions. Moreover, the analysis reveals valuable insights into how spatial spillovers influence regional innovation. The impact of spatial connections varies across the regions, with patents showing the strongest linkages, affecting about 92.27% of regions. Although trademarks and designs have fewer spatial connections (approximately 50% of regions), they still play a significant role in innovation. Although patents have traditionally dominated discussions of innovation, the findings reveal the importance of incorporating designs and trademarks as complementary indicators. Overall, the study highlights the need for multiple metrics to comprehensively evaluate innovations.

Effect of Humic Acids Extracted From Different Organic Sources and Inoculation of Bacillus Subtilis or Aspergillus Niger in Alkaline Phosphatase Activity in Calcareous Soil

Humic acids have received a large attention of researching and development as amendments for poor fertility conditions and deteriorating physical properties, since the process of extracting is an easy common process to implement, as well as dilution of these acids with water enables to be used at economically acceptable levels over large areas. It is necessary to notice that the properties of these acids depending on the organic source and the concentration added to the soil.A laboratory experiment was conducted at the department of soil Sciences and water resources, collage of Agriculture ,University of Basrah ,Iraq to demonstrate the effect of humic acids extracted from different sources and inoculation with Bacillus subtilis or Aspergillus niger on alkaline phosphatase activity in calcareous soil calcareous, southern Iraq. Humic acids extracted from wheat straw, alfalfa leaves, goat manure, and poultry manure which composted previously . Humic acid levels were 0, 25 and 50 L ha-1 .The soil also inoculated with Bacillus subtilis or Aspergillus niger, then samples were incubated at 28±2°C. After 7 or 30 days of incubation the activity of alkaline phosphatase was estimated . Enzyme phosphatase activity was increased in soils treated with humic acid as compared to control with highest activity at Poultry manure (391.54 µg p-nitrophenol gm-1 soil.1h-1).The results also indicated that increasing the level of humic acid significantly increased enzyme activity. For soil treated with Bacillus subtilis and Aspergillus niger , phosphatase activity increased-as compared to control with a higher activity at Bacilluas subtilis (373.1 µg p-nitrophenol gm-1 soil.1h-1).