Bauru School Research Articles

Tomographic analysis of relationship of mandibular morphology and third molars eruption

BackgroundLower third molars (L3M) are the last teeth to erupt in the oral cavity. Uneruption of these teeth still raises questions about its causes, in the literature (1) genetic factors, (2) dental lamina activity and, mainly, (3) insufficient growth and development of the bone bases are included. While the lack of space theory influenced by mandibular morphology and size of L3M was argued to be the main reason for L3M impaction, there is a limitation in the literature in examining such association using more accurate tomographic analysis obtained from CBCT. This work aimed to evaluate the relationship between mandibular morphology and the eruption of L3M.MethodsIn this regard, 85 Cone Beam Computed Tomographies (CBCT), with 147 L3M, were selected from the archives of the Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry, obtained using an Accuitomo® Morita device and using the Dolphin Imaging 11.9 software. L3M eruption was related to linear measurements of jaw length (Co-Gn), retromolar space dimension (D2R), mesiodistal width of the L3M crowns, mandibular first molars (L1M) and mandibular canines (LC) and the angle mandibular (Ar-Go-Me). Independent samples t-test, chi-square tests and logistic regression were performed adopting a significance level of 5%.ResultsThe average mandible length of 116.446 mm + 6.415 mm, retromolar space of 11.634 mm + 2.385 mm, mesiodistal size of the L3M of 10.054 mm + 0.941 mm, sum of the mesiodistal widths of the L1M and LC of 15.564 mm + 1.218 mm and mandibular angle of 127.23° + 6.109. There was no statistically significant association between these factors and the eruption.ConclusionWith the results obtained in this study, we conclude that the length and angle of the mandible, teeth size and dimension of the retromolar space are not associated with the L3M eruption.

Impact of the absence of dental support on cancer patients during COVID-19 pandemic: a cross-sectional study.

PurposeThis study identifies the impact of the absence of dental support for patients with cancer whose clinical dental care was interrupted by the coronavirus disease 2019 (COVID-19) pandemic.MethodsIndividuals with oncologic diseases were selected from a telephone list of a Clinical Research Center (CRC) that specialized in the care of patients with cancer at the Bauru School of Dentistry, University of São Paulo (FOB/USP). The convenience sample comprised 280 patients (aged > 18 years) with a history of cancer that underwent dental treatment at the FOB/USP CRC in 2019 and did not receive care in 2020 owing to the pandemic. The participants completed a questionnaire sent via email or a text messaging application. Individuals receiving treatment or who were already treated for cancer were divided into two groups for data tabulation. Statistical analyses were performed using Fisher’s and chi-square tests.ResultsOf the 280 patients, 104 answered the questionnaire, and 75 (72.1%) were women. Among the women, 45 (60.0%) were receiving antineoplastic treatment, and 30 (40.0%) had already been treated. Among the men, 15 (51.7%) were receiving antineoplastic treatment, and 14 (48.3%) had already been treated. Regarding oral problems that arose during the pandemic, dental pain when eating hot or cold food or drinks (57.0%), muscle pain (53.8%), and difficulties when chewing (51.0%) were the most common reported among patients. Furthermore, most individuals reported not having received any type of remote dental follow-up, before being contacted by our team, which could contribute to reducing these oral problems.ConclusionIt is impossible to say whether the absence of dental support in cancer patients during the coronavirus disease 2019 (COVID-19) pandemic had a negative impact on oral issue rates.

Psychophysiological Reactions of Internet Users Exposed to Fluoride Information and Disinformation: Protocol for a Randomized Controlled Trial.

BackgroundFalse messages on the internet continually propagate possible adverse effects of fluoridated oral care products and water, despite their essential role in preventing and controlling dental caries.ObjectiveThis study aims to evaluate the patterns of psychophysiological reactions of adults after the consumption of internet-based fluoride-related information and disinformation.MethodsA 2-armed, single-blinded, parallel, and randomized controlled trial will be conducted with 58 parents or caregivers of children who attend the Clinics of Pediatric Dentistry at the Bauru School of Dentistry, considering an attrition of 10% and a significance level of 5%. The participants will be randomized into test and intervention groups, being respectively exposed to fluoride-related information and disinformation presented on a computer with simultaneous monitoring of their psychophysiological reactions, including analysis of their heart rates (HRs) and 7 facial features (mouth outer, mouth corner, eye area, eyebrow activity, face area, face motion, and facial center of mass). Then, participants will respond to questions about the utility and truthfulness of content, their emotional state after the experiment, eHealth literacy, oral health knowledge, and socioeconomic characteristics. The Shapiro-Wilk and Levene tests will be used to determine the normality and homogeneity of the data, which could lead to further statistical analyses for elucidating significant differences between groups, using parametric (Student t test) or nonparametric (Mann-Whitney U test) analyses. Moreover, multiple logistic regression models will be developed to evaluate the association of distinct variables with the psychophysiological aspects. Only factors with significant Wald statistics in the simple analysis will be included in the multiple models (P<.2). Furthermore, receiver operating characteristic curve analysis will be performed to determine the accuracy of the remote HR with respect to the measured HR. For all analyses, P<.05 will be considered significant.ResultsFrom June 2022, parents and caregivers who frequent the Clinics of Pediatric Dentistry at the Bauru School of Dentistry will be invited to participate in the study and will be randomized into 1 of the 2 groups (control or intervention). Data collection is expected to be completed in December 2023. Subsequently, the authors will analyze the data and publish the findings of the clinical trial by June 2024.ConclusionsThis randomized controlled trial aims to elucidate differences between psychophysiological patterns of adults exposed to true or false oral health content. This evidence may support the development of further studies and digital strategies, such as neural network models to automatically detect disinformation available on the internet.Trial RegistrationBrazilian Clinical Trials Registry (RBR-7q4ymr2) U1111-1263-8227; https://tinyurl.com/2kf73t3dInternational Registered Report Identifier (IRRID)PRR1-10.2196/39133

Systemic Alterations in patients submitted to exodontics at the Bauru School of Dentistry (USP)

Objective: To verify the rate of systemic changes observed in individuals seen at the Oral and Maxillofacial Surgery and Traumatology clinics of the Undergraduate Dentistry course at the Faculty of Dentistry of Bauru (FOB-USP). Methodology: a retrospective study of the medical records of individuals seen at the Oral and Maxillofacial Surgery and Traumatology clinics of the third and fourth years of the Dentistry course at FOB-USP, from January 1, 2015, to June 30, 2017. Data collection was performed based on information from the anamnesis obtained during the initial care of the patients and recorded in the medical records. The selected data were name, age, address, diagnosed systemic disease, current and/or past medical treatments, proposed surgical treatment, and, if there was, patient return after medical treatment for surgery. The medical records of individuals under the age of 18, patients who did not have any systemic changes, or those with systemic changes observed outside the initial care period were excluded. RESULTS: The sample consisted of 266 medical records and 119 of these were from individuals who had systemic changes (44.7%), with systemic arterial hypertension being the most common (33.1%), followed by diabetes mellitus (17.8%). Conclusion: the high prevalence of individuals with systemic alterations and who require dental surgical intervention was proven, which implies the need for a correct anamnesis and preoperative evaluation so that these cases are managed to avoid trans and/or post-operative, systemic, and/or local.

Role of inflammatory and pain genes polymorphisms in temporomandibular disorder and pressure pain sensitivity

ObjectiveThe aim of this study was to assess the correlation of inflammatory and pain genes polymorphisms with the presence of temporomandibular disorder (TMD) patients and with pressure pain sensitivity. DesignData was collected from 268 consecutive subjects at Bauru School of Dentistry. Subjects aged younger than 20 years, with dental and neuropathic pain, sinusitis, cognitive and neurologic disorder were excluded. Included subjects were evaluated using the Research Diagnostic Criteria for Temporomandibular disorders and divided into two groups: TMD cases and healthy controls. Groups were submitted to pressure pain threshold (PPT) test for the temporomandibular joint, anterior temporalis and masseter muscles and genotyped for Val158Met, IL6−174, IL-1β-3954 and TNFA-308. Student’s t-test and Pearson chi-square test were used to comparisons between groups. A linear multiple regression was used to evaluate the influence of genetics variables on the PPT and a bivariate analysis was used to assesses the influence of genetics variables on pain sensitivity below the PPT cut off of the structures in TMD group. ResultsTMD group showed significantly lower PPT values for all structures when compared with control group (p < 0.001). SNP IL6−174 predicted higher pain sensitivity in the temporomandibular joint (p < 0.005) and in anterior temporalis muscle (p < 0.044) and SNP Val158Met in the masseter muscle (p < 0.038); when TMD group was divided according to PPT cut-off values the SNP Val158Met influenced increase pain sensibility in the masseter muscle. ConclusionTNFA-308 was associated with TMD and SNP IL6−174 and SNP Val158Met influenced pain sensitivity of patients with TMD.

Educação mediada por tecnologia em disfagia orofaríngea: proposta de ensino na graduação

O termo disfagia refere-se a um sintoma diante de alguma dificuldade no processo de deglutição, causada por disfunção esofágica ou orofaríngea, podendo ocasionar insuficiência nutricional e de hidratação. Sendo assim, é importante a atuação fonoaudiológica; porém, para a realização eficiente do tratamento de indivíduos disfágicos, é necessária formação específica. Com o aumento do uso de tecnologias nas áreas de telecomunicações, tornou-se possível o ensino transmitido por meio de tecnologias de informação e comunicação: a Educação a Distância. O objetivo deste estudo foi verificar a efetividade do uso do ensino a distância, associado ao presencial, no processo de aprendizagem de disfagia dos estudantes de graduação em Fonoaudiologia da Faculdade de Odontologia de Bauru – USP. Assim, participaram desta pesquisa estudantes que tiveram contato com a disciplina apenas de forma presencial, e outros que, além do ensino presencial, tiveram acesso à Plataforma Moodle. Todos os estudantes responderam um questionário com perguntas sobre o conteúdo abordado, e foi aplicado um protocolo de avaliação de habilidades e competências. Além disso, opinaram sobre o material educacional on-line. Na análise dos resultados nos questionários houve diferença estatisticamente significante entre notas pré e pós-disciplina apenas para os estudantes do grupo de ensino híbrido. Quanto ao protocolo de habilidades e competências, as notas atribuídas em cada aspecto foram semelhantes para estudantes de ambos os grupos. No que se refere àpercepção dos estudantes quanto ao material on-line, todos referiram que esse ajudou em sua formação. Com isso, conclui-se que o ensino foi efetivo para essa população.

Percepção de estudantes de graduação sobre a importância da disciplina Odontologia para pacientes com necessidades especiais

Pessoas com necessidades especiais (PNE) representam 10% da população mundial e suas particularidades, somadas à falta de conhecimentos teóricos e experiência clínica, fazem com que os cirurgiões-dentistas se sintam inaptos para atendê-los. Sendo assim, o objetivo desse estudo foi identificar a importância percebida pelos estudantes de Odontologia sobre a disciplina Odontologia para Pacientes com Necessidades Especiais (OPNE). Trata-se de um estudo exploratório, descritivo e qualitativo, que realizou a coleta de dados por meio de questionários aplicados a 29 estudantes ao final da disciplina optativa de OPNE da Faculdade de Odontologia de Bauru (FOB). Os estudantes reconhecem que a ausência de contato com PNE durante o curso acarretará insegurança profissional para realizar o atendimento odontológico desses pacientes, dificultando assim o acesso a saúde bucal desta população. A disciplina de OPNE, neste estudo, possibilitou ao estudante de aprender, conviver e realizar atendimento odontológico adequado e humanizado ao PNE.

Efeitos dos agrotóxicos na saúde auditiva de trabalhadores rurais

Introdução: O agrotóxico é um assunto complexo e está pulverizado em diversas instituições e ações, tanto no âmbito do SUS como em outros órgãos de governo, necessitando urgente de constituição de um grupo para viabilizar a articulação das diferentes instâncias. Objetivo: verificar os efeitos da contaminação por agrotóxicos na saúde auditiva de trabalhadores rurais. Material e Métodos: Participaram 8 trabalhadores rurais com no mínimo dois anos de experiência. Os trabalhadores selecionados foram submetidos a dois questionários e avaliação audiológica, por meio dos seguintes procedimentos: entrevista específica, questionário de qualidade de vida no trabalho, audiometria tonal liminar e de alta frequência, logoaudiometria, imitanciometria. A coleta de dados foi realizada na Clinica de Fonoaudiologia da Faculdade de Odontologia de Bauru-Universidade de São Paulo. Resultados: Na avaliação audiológica os resultados encontrados mostraram normalidade, de acordo com padrões pré-estabelecidos, entretanto os participantes foram orientados a realizar acompanhamento devido à faixa limite de normalidade na audiometria convencional e altas frequências, assim como a presença de recrutamento em alguns indivíduos. Conclusão: Os resultados sugerem que as exposições constantes aos agrotóxicos podem causar alterações do sistema auditivo periférico, mesmo que não haja presença de ruído no local de trabalho. Este estudo, e muitos outros, propõem dados que demonstram a importância de programas de prevenção quanto à exposição ao agrotóxico na saúde do trabalhador, implementar o controle dos agentes, assim como a vigilância. Outros trabalhos com uma população mais robusta devem ser desenvolvidos de forma a expor as evidências de comprometimento periférico.

Parental perspectives on early childhood caries: A qualitative study.

Early childhood caries (ECC) affects about 621 million children worldwide, causing chronic pain, infections, sleeping disorders, and tooth extraction. To determine perspectives of parents and caregivers of preschoolers regarding ECC. Three focus groups were conducted with attendees of the Clinics of Paediatric Dentistry from the Bauru School of Dentistry. The meetings were video- and audio-recorded, and the contents were transcribed verbatim. Then, textual passages were examined and coded using the software NVivo 12 Plus. The participants associated the presence of dental caries lesions with negative consequences for children, such as problems for permanent dentition, discrimination, and psychological damages. Although regular oral hygiene and healthy diet were recognized as important factors for the prevention of ECC, specific doubts about feeding, toothpaste usage, oral hygiene, and dental eruption were detected among parents. Additionally, they declared that their children brush teeth alone, intake fermentable carbohydrates frequently, and sleep during breastfeeding without dental plaque removal, because uncooperativeness, permissiveness, and convenience. Based on these results, parents and caregivers understand the unfavourable effects of ECC on children's quality of life; however, their attitudes against the disease seemed to be inaccurate, influenced by their daily routines, doubts, and beliefs.

Por que no papel é mais fácil? O que fazemos e o que falamos que fazemos no Projeto Político-Pedagógico de um Curso de Graduação – Reflexões sobre o Curso de Fonoaudiologia da FOB-USP

Introdução: o projeto político-pedagógico (PPP) de um curso de graduação precisa ser realmente abraçado no contexto acadêmico a que pertence, sendo pedagógico por discutir o processo de formação e político por tratar dos fins e valores referentes da universidade. Análises pontuais de PPP auxiliam não somente a instituição analisada, como também outras instituições de ensino em reflexões e ações sobre formação profissional. No entanto, fica sempre a pergunta: o que fazemos em nosso curso é o que realmente está escrito e descrito nas vias documentais? O que está escrito reflete o perfil do egresso que postulamos? Objetivo: analisar se as características atuais do PPP do Curso de Fonoaudiologia da Faculdade de Odontologia de Bauru (FOB), da Universidade de São Paulo (USP) refletem o perfil do egresso a que se deseja formar e o que efetivamente é colocado em prática no curso. Metodologia: este trabalho é o recorte de um dos itens de pesquisa qualitativa (Tese de Livre Docência) realizada por entrevistas semi-estruturadas com membros da Comissão Coordenadora de Curso (CoC-Fono), Chefia de Departamento e Comissão de Graduação da Unidade; as entrevistas conduzidas foram gravadas, transcritas e submetidas à análise de fidedignidade intercodificadores. A análise de conteúdos temático-categorial foi realizada via software NVivo, sendo que a categoria final a ser aqui apresentada é a de características do PPP atual (perfil de formação, base teórica, pontos fortes e fracos, coerência da estrutura curricular, nível aceitação e conhecimento da comunidade acadêmica). Resultados e discussão: a análise realizada identificou que a construção do PPP foi um processo coletivo e longo, mas necessário como dessensibilização da comunidade acadêmica. O perfil do egresso é generalista, embora haja contraditoriamente grande foco nas especialidades, o que reflete que o documento formal mostra mais o que é postulado pelas Diretrizes Curriculares Nacionais do que o realmente desenvolvido na prática. Há definição dos eixos principais, utilização de novas metodologias e tentativas de integração de disciplinas pontuais, embora ainda apresentadas em estrutura de grade, com alta carga horária – o que entra em conflito com a formação de um profissional reflexivo e flexível postulado no PPP. Houve bom nível de aceitação e satisfação, embora docentes e discentes demonstrem baixo nível de conhecimento sobre o PPP – o que parece contraditório, pois não se aprova ou reprova aquilo que não se conhece. Perspectivas futuras se relacionam à nova proposta de reformulação, com expectativa quanto ao método modular e tutorial para contemplar as necessidades do curso, sociedade atual e perfil do aluno ingressante. Considerações finais: embora seja alto o nível de excelência da formação do fonoaudiólogo na FOB-USP, foi possível verificar que o atual PPP reflete de forma parcial o que tem sido realizado no curso, sendo que há dúvidas dos docentes sobre a adequação do perfil do egresso à real proposta do curso. O fato do processo de elaboração do PPP ter sido coletivo e haver mudanças prévias habilita o curso para novas mudanças curriculares, de forma que seja oferecido de maneira integrada, ensejando as atuais propostas da USP para o ensino de graduação.

PP - INTRAOSSEOUS MANDIBULAR LIPOMA: A DIAGNOSTIC CHALLENGE

A 20-year-old white female presented at FAESA Dental School, with the chief complain of a painless swelling at the right mandible. The lesion was present since she was 12 years old and progressed in the last 2 years. Her medical history was unremarkable. Intraoral examination evidenced a firm, round swelling at the right inferior premolar region, with slight teeth displacement. Occlusal radiography and computed tomography showed low radiopacity and evident cortical expansion at both buccal and lingual aspects of the mandible. An incisional biopsy was performed and the specimen analyzed at the Pathology Service of the Bauru School of Dentistry. Histopathological analysis revealed extensive areas of mature, S-100 positive adipose tissue, interspersed with trabecular viable bone without capsular tissue. Based on clinical, radiographic and microscopic findings, the final diagnosis was of intraosseous lipoma. The patient was submitted to surgical removal of the lesion, preserving the teeth, and is being monitored regularly. Although extremely rare in the jaws, the histopathological analysis is of utmost importance in differential diagnosis of intraosseous lipoma and similar pathologies of the jaws.

Agreement of the International Classification of Sleep Disorders Criteria with polysomnography for sleep bruxism diagnosis: A preliminary study

Statement of problemValidated questionnaires and guidelines for assessing sleep bruxism (SB) that can be administered by dentists in clinical practice are still lacking. PurposeThe purpose of this preliminary study was to compare the third edition of the International Classification of Sleep Disorders (ICSD-3) criteria for diagnosing SB with the results of the gold standard polysomnography (PSG) examination. Material and methodsTwenty consecutive postgraduate students and staff at Bauru School of Dentistry, University of São Paulo, Bauru, Brazil, participated. Each participant underwent interview, clinical assessment, and a PSG evaluation. Bruxers and nonbruxers were identified based only on the PSG analysis. The validity of the ICSD-3 criteria was assessed by receiver operating characteristics curve analysis, area under the curve (AUC), likelihood ratios (LR), and the diagnostic odds ratio (DOR). ResultsThe ICSD-3 diagnostic criteria items for SB had fair to moderate concordance with the PSG diagnosis, with AUC ranging from 0.55 to 0.75. The best value of agreement was the association of SB more than once a week with transient morning jaw muscle pain or fatigue with a moderate but significant agreement with the PSG diagnosis of SB (AUC=0.75), with 90% specificity, positive LR=6, and DOR=13.5. When the frequency of self-reported SB increased to more than 4 times a week, the combination of this finding with tooth wear also had high values of agreement with the PSG diagnosis of SB (AUC= 0.75, +LR=6, DOR=13.6). ConclusionsThe report of regular or frequent SB and the presence of (1) incident of abnormal tooth wear or (2) incidents of transient morning jaw muscle pain or fatigue were the best discriminatory items of ICSD-3 for SB diagnosis.

Diagnostic validity of the use of a portable single-channel electromyography device for sleep bruxism.

The aim of this study was to determine an appropriate cutoff value and the number of nights of sleep with the portable single-channel EMG device (GrindCare) necessary for a valid sleep bruxism (SB) diagnosis. Twenty consecutive post-graduate students and staff at Bauru School of Dentistry composed the sample. Each participant underwent the GrindCare for five consecutive nights and the polysomnography (PSG). The discrimination between bruxers and non-bruxers was based only on the PSG analysis. Data about electromyography per hour with GrindCare (EMG/h) and PSG (bursts/h) were scored. There were positive correlations between the two devices for EMG/h and bursts/h in three and five consecutive nights. Bland-Altman analysis of the EMG bursts/h showed positive agreement between the methods. The receiver operating characteristic (ROC) analyses also showed that using a minimum of 18 EMG/h for three nights and 19 EMG/h for five nights in GrindCare as cutoffs resulted in a 90% specificity and positive likelihood ratio equal to 5. GrindCare is able to discriminate SB diagnosed by PSG and gold standard criteria, when used for three or five consecutive nights, and it may be a valid choice in clinical practice for SB assessment.

A New Anionic Bovine Tendon as Scaffold for the Repair of Bone Defects: A Morphological, Histomorphometric and Immunohistochemical Study

1 Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of Sao Paulo, Al. Dr. Octavio Pinheiro Brisola 9-75, 17012-901, Bauru, SP, Brazil. 2 Medical School, Discipline of Human Morphophysiology, University of Marilia (UNIMAR), Marilia, Sao Paulo, Brazil, R. Hygino Muzy Filho, 17525-902, Marilia, SP, Brazil. 3 Department of Basic Sciences (Anatomy), Aracatuba School of Dentistry, Sao Paulo State University (UNESP), R. Jose Bonifacio 1193, 16015-050, Aracatuba, SP, Brazil. Anatomy, Northern Parana State University (UENP), Av. Manoel Ribas 215, 86400-970, Jacarezinho, PR, Brazil. Department of Health Sciences, University of Sacred Heart, R. Irma Arminda 10-50, 17011-160, Bauru, SP, Brazil. 6 Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), Av. Brigadeiro Faria Lima 5416, 15090-000, Sao Jose do Rio Preto, SP, Brazil.

Conhecimentos e necessidades dos professores em relação aos transtornos do espectro autístico

De acordo às normativas do Ministério da Educação, indivíduos com deficiência e necessidades educacionais especiais devem frequentar classes regulares visando à inclusão destes no sistema educacional. Portanto, é de extrema relevância que o professor seja sempre capacitado a atender as demandas de seus alunos no que tange aos processos de aprendizagem. Este estudo faz parte de um projeto maior com objetivo de utilizar recursos de teleducação como estratégia de ação para prover informações aos professores do ensino infantil visando à inclusão de crianças com Transtornos do Espectro Autístico (TEA) na rede regular de ensino. Este trabalho constou da aplicação e análise de um questionário, realizado com 38 professores de ensino infantil da rede pública municipal de Bauru para investigar as experiências dos professores em relação aos TEA e necessidades de conteúdos sobre a temática. De posse a este conhecimento, foram organizados conteúdos programáticos para elaboração um curso de difusão de conhecimentos que será desenvolvido em uma próxima etapa. Os resultados evidenciaram que a inclusão escolar está em processo de crescimento, porém com professores carentes por informações. Com o estudo, foi possível obter uma maior integração entre a comunidade fonoaudiológica e pedagógica, favorecendo a elaboração do conteúdo de um curso de difusão para os professores que vise à inclusão dos alunos com TEA na rede regular de ensino.

CCR5-dependent recruitment of T cells and regulatory T cells contribute to squamous cell carcinoma development

Event Abstract Back to Event CCR5-dependent recruitment of T cells and regulatory T cells contribute to squamous cell carcinoma development Carine E. Oliveira1, Thais H. Gasparoto1, Caudia R. Pinheiro1, Karen A. Cavassani2, Gustavo P. Garlet1, João S. Silva3 and Ana P. Campanelli1* 1 Bauru School of Dentistry – University of São Paulo, Department of Biological Sciences, Brazil 2 Medical School, University of Michigan, Department of Pathology, United States 3 School of Medicine of Ribeirão Preto – University of São Paulo, Department of Biochemistry and Immunology , Brazil Squamous cell carcinoma (SCC) is one of the most common human cancers worldwide. Recent work show that Treg cells have a critical role in modulating the antitumor immune responses and consequently the SCC development. Because the accumulation of Tregs at the tumor site is due in part to selective recruitment through the CCR5 and the CCR5-associated chemokines, we investigated the role of CCR5 in the SCC development. The results showed that CCR5 deficient mice are more efficient in controlling papilloma incidence than wild-type (WT) mice. In the absence of CCR5, the percentage of leukocytes in the lymph nodes and tumor was significantly increased. Moreover, the adoptive transfer of CD4+CD25+CCR5+, CD4+CD25−CCR5+ and CD8+CCR5+ T cells to CCR5−/− mice resulted in an increased papilloma incidence. Interestingly, adoptive transfer of Treg cells induced a SCC lesion more undifferentiated than all other groups and a higher infiltration of macrophage, dendritic cells and myeloid suppressive cells. Overall, CCR5 is a key receptor for the migration of T cells to the SCC lesions. Thus, a tight control of CCR5+ T cells migration, especially Treg cell, to the SCC lesions could be an important mechanism for avoiding tumor progression. Keywords: Squamous cell carcinoma, chemokine, regulatory T cell., CCR5, tumor Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Oliveira CE, Gasparoto TH, Pinheiro CR, Cavassani KA, Garlet GP, Silva JS and Campanelli AP (2013). CCR5-dependent recruitment of T cells and regulatory T cells contribute to squamous cell carcinoma development. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00878 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Ana P Campanelli, Bauru School of Dentistry – University of São Paulo, Department of Biological Sciences, Bauru, Brazil, apcampan@usp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Carine E Oliveira Thais H Gasparoto Caudia R Pinheiro Karen A Cavassani Gustavo P Garlet João S Silva Ana P Campanelli Google Carine E Oliveira Thais H Gasparoto Caudia R Pinheiro Karen A Cavassani Gustavo P Garlet João S Silva Ana P Campanelli Google Scholar Carine E Oliveira Thais H Gasparoto Caudia R Pinheiro Karen A Cavassani Gustavo P Garlet João S Silva Ana P Campanelli PubMed Carine E Oliveira Thais H Gasparoto Caudia R Pinheiro Karen A Cavassani Gustavo P Garlet João S Silva Ana P Campanelli Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

C. albicans phagocytosis by mast cells via Toll like receptor-2: protection or immune escape?

Event Abstract Back to Event C. albicans phagocytosis by mast cells via Toll like receptor-2: protection or immune escape? Karen H. Pinke1*, Heliton G. Lima1, Fernando Q. Cunha2 and Vanessa S. Lara1 1 Bauru School of Dentistry. University of São Paulo., Department of Stomatology, area of Pathology, Brazil 2 Faculty of Medicine of Ribeirão Preto. University of São Paulo., Department of Pharmacology., Brazil Candida albicans (C. albicans) is a fungus commonly found in the mucosa of the gastrointestinal tract, including the mouth, and may causecandidiasis, especially in immunosuppression. Mast cells reside in the subepithelial region and actively participate in innate immunity, like one of the first lines of defense against C. albicans. The recognition of this fungus can occur via different receptors, e.g. Toll-like receptor 2 (TLR2), present on the surface of mast cells. Thus, this study evaluated in vitro the relationship of the phagocytosis, intracellular oxide nitric production by mast cells challenged with C. albicans and the involvement of TLR2 in these mechanisms. For this, Murine bone marrow cells (BMMC) wild type (TLR2+ /+) or knockout (TLR2-/-) were cultured in presence of stem cell factor and interleukin-3. After, the BMMCs were challenged with FITC-labeled C. albicans by 60 minutes and the phagocytosis analyzed by confocal laser scanning microscopy. Furthermore, intracellular nitric oxide production was measured used the DAF-FM diacetate and spectrophotometer. Our results showed that BMMC TLR2+ /+ phagocytose C. albicans but did not showed overproduction of the nitric oxide after stimulation with fungus, in relation with the basal production. However, in the absence of the TLR2, the nitric oxide production is increased, while the phagocytosis is reduced, in comparison with wild mouse. Therefore, we conclude that the C. albicans recognition by TLR2 results in the increased phagocytosis, but without nitric oxide stimulous. This can represent an escape way of the fungus of the defense mechanisms developed by mast cells via TLR2. Keywords: Mast Cells, immune escape, Candida albicans, TLR2 signaling, Nitric Oxide Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Pinke KH, Lima HG, Cunha FQ and Lara VS (2013). C. albicans phagocytosis by mast cells via Toll like receptor-2: protection or immune escape?. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00054 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Karen H Pinke, Bauru School of Dentistry. University of São Paulo., Department of Stomatology, area of Pathology, Bauru, Brazil, karen.pinke@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Karen H Pinke Heliton G Lima Fernando Q Cunha Vanessa S Lara Google Karen H Pinke Heliton G Lima Fernando Q Cunha Vanessa S Lara Google Scholar Karen H Pinke Heliton G Lima Fernando Q Cunha Vanessa S Lara PubMed Karen H Pinke Heliton G Lima Fernando Q Cunha Vanessa S Lara Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Influence of tinnitus on pain severity and quality of life in patients with temporomandibular disorders

Objective The aim of this cross-sectional study was to evaluate the relationship among pain intensity and duration, presence of tinnitus and quality of life in patients with chronic temporomandibular disorders (TMD). Material and Methods Fifty-nine female patients presenting with chronic TMD were selected from those seeking for treatment at the Bauru School of Dentistry Orofacial Pain Center. Patients were submitted to the Research Diagnostic Criteria anamnesis and physical examination. Visual analog scale was used to evaluate the pain intensity while pain duration was assessed by interview. Oral Health Impact Profile inventory modified for patients with orofacial pain was used to evaluate the patients' quality of life. The presence of tinnitus was assessed by self report. The patients were divided into: with or without self report of tinnitus. The data were analyzed statistically using the Student's t-test and Pearson's Chi-square test, with a level of significance of 5%. Results The mean age for the sample was 35.25 years, without statistically significant difference between groups. Thirty-two patients (54.24%) reported the presence of tinnitus. The mean pain intensity by visual analog scale was 77.10 and 73.74 for the groups with and without tinnitus, respectively. The mean pain duration was 76.12 months and 65.11 months for the groups with and without tinnitus, respectively. The mean OHIP score was 11.72 and 11.74 for the groups with and without tinnitus, respectively. There was no statistically significant difference between groups for pain intensity, pain duration and OHIP scoreS (p>0.05). Conclusion Chronic TMD pain seems to play a more significant role in patient's quality of life than the presence of tinnitus.

The 50th anniversary and the 25th Dental Congress of the Bauru School of Dentistry of the University of São Paulo

The 50th anniversary and the 25th Dental Congress of the Bauru School of Dentistry of the University of São Paulo

Bauru School of Dentistry Tele-Health League: an educational strategy applied to research, teaching and extension among applications in tele-health

Tele-health is more than an innovative alternative; it is an excellent tool that enables access to health and education in health, making it possible to minimize distances, optimize time and reduce costs. Based on these advantages, some Brazilian Universities have used these actions in strategies of education, research and extension, aiming at the application of Tele-health in Brazil. In that way, the Bauru School of Dentistry - University of São Paulo (FOB-USP) has applied the use of information and communication technologies in health by means of a "Tele-Health League" (TL), in order to diagnose, prevent and treat diseases, in addition to educate the population and health services.ObjectiveThe present study aims to introduce the characteristics of the Tele-Health League of FOB-USP, as well as the development of its projects. Material and MethodsThe Tele-Health League consisted as a Diffusion Course approved by the Provost of Culture and Academic Extension of the University of São Paulo. It is composed as a large group enclosing professoriate coordinator, academician principal, contributing professors and league members, those, diversified between undergraduates students, graduated, health employees, technology and information areas. The participant members are evaluated by the presence frequency (minimum of 85%), and by the performance of tests and paperwork about the theoretical content provided. ResultsIn four years of activities, the TLFOB-USP obtained a high satisfaction index (90%), an increased number of vacancies due to the interest to become a member, more commitment of the professors of the University and the accomplishment of association with other Brazilian leagues. It is emphasized that the approval percentage of the course results in approval from approximately half of its members. Also, it is important to identify and repair the causes related to the quitting of some members. ConclusionsThe results showed that the TLFOB-USP members, adjoining to the professor's participants, develop projects in Tele-helth, in Tele-aid and Tele-education areas, thus resulting in the involvement of the University and the community.