Scenedesmus Dimorphus Research Articles

Growth potential, biochemical properties and nutrient removal efficiency of some freshwater microalgae and their consortia from wastewater

Impact of varying nitrate (NO3-N) and phosphate (PO4-P) concentrations and sewage water (SW) on the growth, nutrient removal, lipid accumulation, enzymatic antioxidant activity and phytochemical contents of the microalgae Scenedesmus dimorphus, Coelastrella tenuitheca, Chroococcus turgidus and Parachlorella kessleri under monoculture and their consortia have been investigated. High growth rates were observed for all the four algae in both mono and mixed culture conditions at enhanced concentrations of N (1500 mg/L NO3-N) and P (40 mg/L PO4-P). The species Scenedesmus dimorphus outperformed other microalgae growing in SW in efficiently removing nitrogen. The algal consortia of mixed species was found to be more effective in phosphorus removal. The carbohydrate and protein contents were highest in Parachlorella kessleri, about 37% and 44%, respectively, in SW cultivation. The algal consortia demonstrated highest starch content (4%) in nitrogen deprived growth medium. Highest lipid production (43%) was observed in the SW culture. The species Coelastrella tenuitheca, Chroococcus turgidus and Scenedesmus dimorphus irrespective of the growth media indicated significant accumulation of phenol, flavonoid and tannin. The DPPH, catalase and ascorbic peroxidase assay showed pronounced antioxidant activity. Nutrient (N and P) enrichment exhibited enhanced antioxidant enzymatic activity and accumulation of cell storage products.

Improvising Biodiesel Production from Scenedesmus dimorphus via Nutrient Starvation and Optimized Pretreatment Process

Improvising Biodiesel Production from Scenedesmus dimorphus via Nutrient Starvation and Optimized Pretreatment Process

Molecular Phylogeny of Chlorophyta Species Isolated from a Shallow Lake Ecosystem

Studying the algal diversity of lentic ecosystems is an important issue for identifying the alteration in aquatic ecosystems. Morphological characterization of algae is inadequate to identify different types of microalgae and it is also necessary to depend on molecular evidence which is more crucial and a precise tool. In this study, two Chlorophyta species were identified; Tetradesmus dimorphus (Turpin) M.J.Wynne (Basionym: Scenedesmus dimorphus) and Chlorella sorokiniana Shihira & R.W.Krauss), in a shallow lake. The evolutionary tree was constructed for both species and the results showed that the Iraqi Tetradesmus dimorphus isolate was placed in clade 4, more closely related to the Tetradesmus dimorphus from USA, China, Pakistan, and Sweden with similarity 99 %, 99%, 97 %, and 96 % for all respectively. The Iraqi isolated Chlorella sorokiniana (OP718564.1) is within the clade 2, and composed sister group with Chlorella sorokiniana (KU29188.1) from the USA, with 99 % similarity.

Accessing biochemical shifts in a novel Scenedesmus strain via acetaminophen detoxification: Experiment utilizing Box-Behnken optimization and isotherm analysis

Acetaminophen's inherent solubility and hydrophilic nature facilitate its accumulation in aquatic ecosystems. Herein, Scenedesmus dimorphus IITISM-DIX1 demonstrates efficient acetaminophen removal, concurrently serving as a substrate for lipid biosynthesis. Employing Box-Behnken design, optimization of parameters like pH, light duration and concentration of acetaminophen influencing its elimination is executed. Characterization of pre- and post-algal biomass involves FE-SEM, FTIR, and BET analysis. Kinetic and adsorption analyses reveal pseudo-first-order kinetics (R2 = 0.99) and adherence to the Freundlich isotherm (R2 = 0.94). FTIR spectroscopy demonstrates subtle shifts in IR bands post-sorption, indicative of biomass involvement in adsorption processes. Biodegradation and biosorption serve as the main removal pathways, facilitated by exopolysaccharides, generating by-products such as 4-aminophenol, hydroquinone, and formic acid. The Akaike Information Criterion (AIC) and Bayesian Information Criterion (BIC) values obtained for the Freundlich isotherm validate it as the optimal model, indicating heterogeneous multilayered sorption with efficiency ranging from 44% to 100%. Additionally, exposure to acetaminophen-contaminated media leads to biochemical alterations in Scenedesmus dimorphus IITISM-DIX1. The findings of this study unveil the first elucidated pathway for acetaminophen degradation by any Scenedesmus species, delivering essential knowledge about microalgae-mediated acetaminophen degradation and lipid enrichment mechanisms.

Optimization of microalgal biomass (Scenedesmus dimorphus) for maximized bioethanol production through response surface methodology (RSM)

Optimization of microalgal biomass (Scenedesmus dimorphus) for maximized bioethanol production through response surface methodology (RSM)

Microalgae production in olive mill wastewater fractions and cattle digestate slurry: Bioremediation effects and suitability for energy and feed uses

The possibility of obtaining energy or nutritive streams and bioremediation as an add-on opens new perspectives for the massive culturing of microalgal biomass on waste waters generated by the agro-food sector. Ordinary revenue streams are fully preserved, or even boosted, if they are used in microalgal cultivation; however, the suitability of wastewaters depends on multiple nutritional and toxic factors. Here, the effect of modulating the Olive Mill Wastewater (OMW) and cattle digestate (CD) fraction in the formulation of a growth medium on biomass accumulation and productivity of selected biomass fractions and their relevance for biofuel and/or feed production were tested for the microalga Scenedesmus dimorphus and for the cyanobacterium Arthrospira platensis (Spirulina). Tests highlighted the strong S. dimorphus adaptability to digestate, as on OMW, compared to A. platensis, with the maximum lipid storage (48 %) when culture medium was composed by 50 % of cattle digestate.

Effect of organic carbon sources on growth, lipid production and fatty acid profile in mixotrophic culture of Scenedesmus dimorphus (Turpin) Kützing

Due to non-renewability of fossil fuels and various environmental problems associated with their use, there is a growing interest in producing biodiesel from microalgae. Finding novel microalgal strains with desired characteristics such as rapid growth rates, high lipid productivity, and suitable fatty acid compositions is important for the production of biodiesel. Comparatively, photoautotrophic cultivation of microalgae results in low biomass yields and lipid productivities. Mixotrophic and heterotrophic cultures, in contrast, lead to higher biomass and lipid productivities but their productivities are species specific and depend a lot on the organic carbon source and culture conditions. In the present study, the potential of Scenedesmus dimorphus for biodiesel oil production under mixotrophic culture was investigated. The effects of organic carbon sources (glucose, ethanol and glycerol) on growth, biomass yields, lipid contents, lipid productivities and fatty acids compositions of Scenedesmus dimorphus in mixotrophic cultures were investigated. In comparison to photoautotrophic cultures, mixotrophic cultures produced significantly higher biomass concentration, lipid content, and lipid productivities (P≤0.05) under all the cultivation conditions investigated. The maximum biomass and lipid productivities achieved by mixotrophic culture were 83.88±0.05 and 17.11±0.04 mg L−1 day−1 respectively which are more than 2.6 and 3.1 folds higher than those obtained under photoautotrophic condition. Using glucose as the organic carbon source was better than ethanol and glycerol in terms of biomass and lipid productivities (glucose > ethanol > glycerol). The majority of the produced fatty acids were composed of C16 and C18 fatty acids. The two most abundant fatty acids in all the cultures were linolenic and palmitic acids. The values of biodiesel properties of oil obtained from S. dimorphus are within the American (ASTM D6751) and European (EN 14214) biodiesel specifications. The results show that S. dimorphus has good potentials for biodiesel oil production and that the productivity can be enhanced by mixotrophic cultivation.

Microalgae cultivation using ammonia and carbon dioxide concentrations typical of pig barns

ABSTRACT While global population growth drives increased production efficiency in animal agriculture, there is a growing demand for environmentally friendly practices, particularly in reducing air pollutant emissions from concentrated animal feeding operations. This study explores the potential of cultivating microalgae in photobioreactors (PBRs) as an eco-friendly and cost-effective approach to mitigate NH3 and CO2 emissions from pig barns. Unlike traditional physicochemical mitigation systems, microalgae offer a renewable solution by converting pollutants into valuable biomass. The research focused on Scenedesmus dimorphus growth under typical NH3 and CO2 concentrations found in the indoor air of pig barns. Four NH3 (0, 12, 25, and 50 ppm) and four CO2 concentrations (350, 1200, 2350, and 3500 ppm) were tested using photobioreactors. Results showed a maximum specific growth rate of 0.83 d−1 with 12 ppm NH3 and 3500 ppm CO2. The dry biomass concentration was significantly higher (1.16 ± 0.08 g L−1; p < 0.01) at 25 ppm NH3 and 2350 ppm CO2 than other test conditions. S. dimorphus demonstrated the peak NH3 and CO2 fixation rates (23.8 ± 2.26 mg NH3 L−1 d−1 and 432.24 ± 41.09 mg CO2 L−1 d−1) at 25 ppm NH3 and 2350 ppm CO2. These findings support the feasibility of using algae to effectively remove air pollutants in pig barns, thereby improving indoor air quality.

Physiological responses and removal mechanisms of ciprofloxacin in freshwater microalgae

Antibiotics, such as ciprofloxacin (CIP), are frequently detected in various environmental compartments, posing significant risks to ecosystems and human health. In this study, the physiological responses and elimination mechanisms of CIP in Chlorella sorokiniana and Scenedesmus dimorphus were determined. The exposure CIP had a minimal impact on the growth of microalgae, with maximum inhibit efficiency (IR) of 5.14% and 22.74 for C. sorokiniana and S. dimorphus, respectively. Notably, the photorespiration in S. dimorphus were enhanced. Both microalgae exhibited efficient CIP removal, predominantly through bioaccumulation and biodegradation processes. Intermediates involved in photolysis and biodegradation were analyzed through Liquid Chromatography High Resolution Mass Spectrometer (HPLC-MS/MS), providing insights into degradation pathways of CIP. Upregulation of key enzymes, such as dioxygenase, oxygenase and cytochrome P450, indicated their involvement in the biodegradation of CIP. These findings enhance our understanding of the physiological responses, removal mechanisms, and pathways of CIP in microalgae, facilitating the advancement of microalgae-based wastewater treatment approaches, particularly in antibiotic-contaminated environments.

Boosting Antioxidant Activity and Phenolic Content in Scenedesmus dimorphus

Scenedesmus dimorphus, a microalgae from the Chlorophyceae class, possesses compounds with antioxidant potential. This study focuses on determining the antioxidant activity of the methanol extract of *Scenedesmus dimorphus* cultured in Bold Basal Medium (BBM) with NaCl concentrations of 10 g/L, 12.5 g/L, and 15 g/L. NaCl was added after 3, 6, and 9 days of cultivation to evaluate the impact on antioxidant activity. The antioxidant activity was assessed using the DPPH (1,1-diphenyl-2- picrylhydrazyl) assay. The results showed that the highest antioxidant activity occurred in cultures with 15 g/L NaCl, with DPPH inhibition of 13.71% and a biomass yield of 0.1914 g in 500 mL of medium. The microalgae demonstrated the ability to withstand NaCl stress, showing improved antioxidant activity over time. On day 9, with the addition of 15 g/L NaCl, the antioxidant activity increased to 26.82%, the IC50 value was 183.63 mg/L, and the biomass weight reached 0.2568 g in 500 mL of medium. Additionally, the total phenolic content increased to 70.10 mg GAE/g extract on day 9. These findings suggest that NaCl stress at specific intervals enhances both antioxidant activity and phenolic content in Scenedesmus dimorphus.

Evaluation on microalgae for the production of bio-chemicals and electricity

Recent advancement in biophotovoltaic systems using microalgae, coupled with biorefinery approach, would improve economy-feasibility in production. The major concern is its commercial strength in terms of scalability, strain selection and extraction procedure cost. It must compete with conventional feedstocks such as fossil fuels. This project proposes to enhance the economic feasibility of microalgae-based biorefinery by evaluating their performance for bio-electricity, bio-diesel and carotenoids production in a single cycle. The first part of the study was to construct and select a Bio-bottle Voltaic (BBV) device that would allow microalgae to grow and produce bioproducts, as well as generate the maximum current output reading derived from the microalgae's photosynthesis process. The second phase consisted of a 25-day investigation into the biorefinery performance of six different microalgal species in producing bio-electricity, bio-diesel and carotenoid in a prototype BBV device. The prototype BBV device with aluminium foil and pencil lead as its anode and cathode produced the highest carotenoid and biodiesel component production from the two microalgae tested, according to the results of the first phase of the experiment. In the second portion of the study, Scenedesmus dimorphus and Chlorella vulgaris were identified as the two microalgae most capable of maintaining their growth throughout the experiment. The maximum current reading observed for C. vulgaris was 653 mV. High Performance Liquid Chromatography analysis showed four major carotenoid compounds found which were Neoxanthin, Cantaxanthin, Astaxanthin and 9-cis antheraxanthin, and the highest carotenoid producer was C. vulgaris which recorded at 1.73 μg/mL. C. vulgaris recorded as the most alkanes producer with 22 compounds detected and Heptacosane and Heneicosane as the two major biodiesel compounds found in the extracts. Evaluation of C. vulgaris data showed that it has enormous potential for microalgal biorefinery candidates. Further ongoing research and development efforts for C. vulgaris will improve the economic viability of microalgae-based industries and reduce reliance on depleted fossil fuels.

Microalgae Production on Biogas Digestate in Sub-Alpine Region of Europe—Development of Simple Management Decision Support Tool

In a one-and-a-half-year study conducted in the ALS6 region in Europe (Ljubljana, Slovenia), the cultivation of microalgae in anaerobic digestate from food waste, mainly Scenedesmus dimorphus and Scenedesmus quadricauda, was investigated in three ponds (1260 L each) under a greenhouse. The effects of changing digestate quality and quantity as well as seasonal fluctuations on the productivity of the microalgae were investigated in three stages: Learning/Design (SI), Testing (SII), and Verification/Calibration (SIII). A decision support tool (DST) was developed using easy-to-measure parameters such as pH, temperature, electrical conductivity, mineral nitrogen forms and physical, biological parameters (OD, delayed fluorescence intensity). To control optimal pond operation, we proposed the photosynthetic culture index (PCI) as an early indicator for necessary interventions. Flocculation and nitrite levels (above 3 mg NO2-N L−1) were signals for the immediate remediation of the algae culture. Under optimal conditions in summer SIII, an average algal biomass production of 11 ± 1.5 g m−2 day−1 and a nitrogen use efficiency of 28 ± 2.6 g biomass/g N-input were achieved with the developed DST. The developed DST tool was, in this study, successfully implemented and used for the cultivation of microalgae consortia predominated by Scenedesmus dimorphus and S. quadricauda with biogas digestate. DST offers the possibility to be modified according to producers’ specific needs, facility, digestate and climate conditions, and as such, could be used for different microalgae cultivation processes with biogas digestate as a food source.

Evaluation of Scenedesmus dimorphus under Different Photoperiods with Eutrophicated Lagoon Water

Given the need to improve bioenergy production processes, it is necessary to focus on low-cost culture media and environmental conditions of radiation and temperature. The Scenedesmus dimorphus species was cultured in eutrophicated lagoon water and Bayfolan 0.3% as culture media under four photoperiods with the objective of evaluating the biomass productivity, bioremediation capacity and influence of illumination on the composition and lipid content. It is concluded that the increase of light hours in the culture with eutrophicated lagoon water produces a decrease in the biomass productivity and COD removal percentage. The highest biomass productivity was obtained in photoperiod F1 (10.5:13.5) hours L:O, 0.053 ± 0.0015 g/L day and a removal of 95.6%. Bayfolan 0.3% with F2 (11.5:12.5) and F3 (12.5:11.5) did not show significant differences in the biomass productivity and COD removal. The increase in light hours in the photoperiod induced an increase of 1.01% and 2.84% of saturated fatty acids and 0.8% and 2.14% of monounsaturated fatty acids, as well as a decrease of 3.85% and 2.88% of polyunsaturated fatty acids in eutrophicated lagoon water and Bayfolan 0.3%, respectively.

The effects of different ratios of mixed feeding ofScenedesmus dimorphustoCyclotellasp. on the growth performance, pearl production ability, and biochemical components of the new shell tissue ofHyriopsis cumingii

AbstractIn order to screen out the feeding ratio of high‐quality algae strains inHyriopsis cumingii, this experiment investigated the effects of different ratio of mixed feeding of two algae strains (Scenedesmus dimorphusandCyclotellasp.) on the growth, bead production performance, trace elements, and amino acid composition of the newborn shell ofH. cumingii. The results showed that the mixed feeding of two types of microalgae with different densities had significant effects on the growth performance, bead production quality, trace elements, and amino acid composition of the newly formed shell ofH. cumingii(p &lt; 0.05); the best body weight gain rate and shell growth rate were obtained by feedingS. dimorphus(30 × 105cell/mL) andCyclopellasp.(8 × 105cell/mL); the pearl weight gain rate, amino acid ratio, Ca2+and Mn2+content were best when feedingCyclotellasp. (16 × 105cell/mL), but there was no significant difference compared withS. dimorphus(30 × 105cell/mL) andCyclopellasp.(8 × 105cell/mL). In summary, the optimal feeding amount for the intensive breeding process ofH. cumingiiisS. dimorphus(30 × 105cell/mL) andCyclopellasp. (8 × 105cell/mL).

Construction of an efficient microalgal-fungal co-cultivation system for swine wastewater treatment: Nutrients removal and extracellular polymeric substances (EPS)-mediated aggregated structure formation

Microalgal-fungal co-cultivation technology has demonstrated outstanding efficacy in wastewater treatment and valuable resources recovery. However, it is still unclear how extracellular polymeric substances (EPS) could potentially affect the formation and development of co-cultivation aggregates. In this work, efficient microalgal-fungal co-cultivation systems (MFCS) were developed for swine wastewater treatment. Different co-cultivation systems were established using various microalgae (Chlorella sorokiniana and Scenedesmus dimorphus) with fungi (Aspergillus oryzae or Aspergillus niger). The results showed that the introduction of fungi improved nutrient removal, and C. sorokiniana-A. oryzae co-cultivation system demonstrated the highest removal efficiency for chemical oxygen demand (COD) (85.9 %), ammonium (NH4+-N) (76.5 %), and total phosphorus (TP) (60.3 %). Biomass production in co-cultivation system was higher, and S. dimorphus-A. oryzae co-cultivation system achieved the highest biomass production of 0.74 g/L. In addition, the lipid content in co-cultivation system was up to 33.3 %, which was 1.8 times higher than that in the mono-cultivation group. Besides biomass production, A. oryzae also promoted the photosynthetic activity of C. sorokiniana, showing higher pigments content and electron transport efficiency. The morphology of co-cultivation systems displayed complex interactions between fungi and microalgae, forming aggregated structures. The analysis of EPS demonstrated their importance in the formation and stabilization of microalgae-fungal aggregates. Moreover, proteins and polysaccharides, along with chemical bonds (N-H, C = O and C-O-C), played a crucial role in the formation and development of aggregates. This study provides insights into the potential of MFCS for swine wastewater treatment, highlighting the importance of species selection and EPS-mediated interactions in enhancing system performance.

ALGINATE BEADS UTILIZATION FOR LONG-TERM STORAGE OF MICROALGAL ISOLATES

Microalgae reckon one of the most promising organisms due to their numerous applications in biotechnology, such as, their biomass utilization to extract various primary and secondary metabolites. These compounds benefit the food and pharmaceutical industries and the energy field, such as, biofuel and hydrogen gas production. Therefore, it is necessary to find various sustainable ways to actively preserve the isolates and productive strains with purity for an extended period without affecting their genetic characteristics and productive ability to grow and regenerate. The latest study aimed to compress several microalgae to form alginate beads using sodium alginate with five types of pure algal cultures, i.e., Scenedesmus quadricauda, Scenedesmus dimorphus, Chlorella vulgaris, Chlorococcum humicola, and Chlamydomonas sp. The vitality and activity of all the strains studied through the storage period showed the success of preparing alginate beads and staying viable for 18 months at 4 °C in the refrigerator under dark conditions. Therefore, encapsulating microalgae with sodium alginate is a possible and helpful method for preserving algae isolates for a prolonged period in a pure form. The survival of algae in alginate beads is an essential step to apply in the future as one of the viable methods to preserve pure algae isolates for a long time.

Effects of Scenedesmus dimorphus, spirulina biodiesel, hydrogen and nanoparticles fuel blends on mass burn fraction, emission, noise and vibration characteristics

Severe environmental implications, such as those connected to greenhouse gas emissions and widespread pollution, have resulted from the unsustainable demand for fossil fuels. In order to find and implement technological, sustainable, and cost-effective alternatives to fossil fuel production and use, it is necessary to first identify the primary challenges originating from these activities. The significant potential for their production from various types of biomass has led to a rise in their use in recent years, and this has coincided with the advent of current technological breakthroughs in the field. Scenedesmus dimorphus The energy sector has a strong interest in the process of making biodiesel from and spirulina oil because of its potential to provide clean and sustainable energy.The combustion, emission, vibration and noise characteristics of Scenedesmus Dimorphus and spirulina based microalgae biodiesel were studied in this study, both with and without the addition of nanoparticles and hydrogen. Hydrogen and nanoparticles were employed at a rate of 5 L/min and 100 ppm respectively. Biodiesel blends B10 (5 % spirulina + 5 % Scenedesmus Dimorphus + 90 % diesel) and B20 (10 % spirulina + 10 % Scenedesmus Dimorphus + 90 % diesel) were used in this study. The test fuels prepared from biodiesel, hydrogen and nanoparticles has been compared with conventional diesel.The experiment was carried out on four stroke diesel engine at varying load conditions ranging from 0 % to 100 % at an interval of 25 %. Incorporation of nanoparticles and hydrogen on microalgae based biodiesel reduces the emission, vibration and noise generated by the engine.

Multi-Criteria Decision Analysis for Optimizing CO2 and NH3 Removal by Scenedesmus dimorphus Photobioreactors

Numerous technologies have been investigated for mitigating air pollutant emissions from swine barns. Among them, algal photobioreactors (PBRs) can remove and utilize air pollutants such as CO2 and NH3 from barn exhaust. However, a challenge to PBR operation is that it involves multiple system input parameters and output goals. A key question is then how to determine the appropriate CO2 and NH3 concentrations in this case. Conventional statistical methods are inadequate for handling this complex problem. Multi-criteria decision-making (MCDM) emerges as a practical methodology for comparison and can be utilized to rank different CO2–NH3 interactions based on their environmental and biological performance. By employing MCDM methods, producers can effectively control the ratio of CO2 and NH3 concentrations, enabling them to identify the optimal range of operating parameters for various housing types, ensuring efficient pollutant mitigation. In this study, a multi-criteria decision-making (MCDM) approach was employed to support operation management. Specifically, influent CO2 and NH3 concentrations were optimized for three scenarios (the best biological, environmental, and overall performance), using a combination of two MCDM techniques. This study is anticipated to facilitate the system analysis and optimization of algae-based phytoremediation processes.

Makeup Water Addition Can Affect the Growth of Scenedesmus dimorphus in Photobioreactors

Makeup water constitutes a key component in the water management of microalgal cultivation systems. However, the effect of makeup water addition on microalgal growth remains largely unexplored. This study compared two deionized water addition intervals (1 day and 4 days) for their effect on the growth of Scenedesmus dimorphus (S. dimorphus hereafter) in 2000 mL Pyrex bottles under controlled conditions. Cell counts and dry algal biomass (DAB) were measured to characterize the microalgal growth rate. Water addition intervals impacted algal cell counts but had little effect on DAB. Adding makeup water every day resulted in a higher growth rate (8.80 ± 1.46 × 105 cells mL−1 day−1; p = 0.22, though) and an earlier occurrence of the peak cell count (day 9) than adding it every 4 days (6.95 ± 1.68 × 105 cells mL−1 day−1 and day 12, respectively). It is speculated that water loss over an extended period and the following makeup water addition posed stress on S. dimorphus. Surpassing the peak cell count, S. dimorphus continued to grow in DAB, resulting in an increased cell weight as a response to nutrient starvation. Optical density at 670 nm (OD670) was also measured. Its correlation with DAB was found to be affected by water addition intervals (R2 = 0.955 for 1 day and 0.794 for 4 days), possibly due to a water loss-induced change in chlorophyll a content. This study is expected to facilitate the makeup water management of photobioreactor and open pond cultivation systems.

Heavy metal pollution of river water and eco-friendly remediation using potent microalgal species

Pollution of rivers is mainly caused by anthropogenic activities such as discharge of effluent from industrial facilities, maintenance of sewage/effluent treatment plants, and dumping of solid waste on river banks. This study dealt with the pollution issues of the Cooum River in the well-known city of Chennai in South India. Water samples from 27 locations were collected and analyzed for 12 elements, including Ba, B, and Al, as well as heavy metals such as Pb, Cr, Mn, Fe, Co, Ni, Cu, Zn, and Cd. The samples showed levels of these elements that exceeded World Health Organization recommendations. Pearson correlation analysis revealed the inter-dependency among elements, and the contribution of each element based on factor loadings showed its percentage contribution compared to others. Water samples from six significant locations were chosen for remediation with three algae: Chlorella vulgaris, Scenedesmus dimorphus, and Phormedium sp. The uptake of pollutants led to the continuous growth of algae during the incubation period of 15 d, effectively removing heavy metals from the river water. The increasing levels of algal counts and the chlorophyll a content confirmed the algal growth during the incubation period, followed by a declining stage after the incubation period. The scanning electron microscopic images of algae before and after the remediation showed no remarkable modification of morphological patterns. This study showed that the uptake of heavy metals using algae is an effective water pollution remediation measure, making the process practicable in the field on a large scale in the near future.