Objective: This study aimed to monitor the use of a short-wave ultraviolet (UV-C) decontamination device for N95 masks at the João de Barros Barreto University Hospital, Belém, Pará, Brazil. The research investigated the reuse of masks, storage methods, and the perception of healthcare professionals regarding the decontamination protocol using UV-C after each work shift. Theoretical Framework: The research is based on theories related to biosafety and occupational health, focusing on the importance of decontamination processes in hospital settings to enhance the protection of healthcare workers and reduce infection risks. Method: A cross-sectional study was conducted with 295 nursing professionals, including nurses, technicians, and assistants. Data were collected through a questionnaire addressing the reuse of N95 masks, storage between uses, and opinions on the use of the UV-C device for decontamination. Results and Discussion: All participants (n=295) agreed that UV-C decontamination improves biosafety by reducing the handling of potentially contaminated masks. The acceptance of the device was unanimous, confirming its feasibility both in high-demand scenarios, such as the Covid-19 pandemic, and in routine hospital practices. Research Implications: This study demonstrates that implementing UV-C decontamination systems is a practical and effective solution for hospitals, with the potential to increase occupational safety and reduce infectious waste generation. Originality/Value: The study contributes to the literature by highlighting the feasibility and benefits of reusing N95 masks with UV-C decontamination, promoting greater safety in hospital environments.