Scatter Measurements Research Articles

An analytical observational study for diagnostic accuracy of volume, conductivity & scatter (VCS) indices of neutrophils for diagnosis of sepsis in an emergency hospital setting.

Background & objectives The newer technique using an innovative volume conductivity scatter (VCS) technology is emerging as a surrogate for sepsis diagnosis. The VCS technology offers a more objective method to measure cell volume (V), characterize conductivity (C) and light scatter (S) directly from more than 8,000 white blood cells (WBCs). However, diagnostic performance of VCS parameters in sepsis has not been extensively tested in routine hospital emergency settings. Therefore, the present study aimed to investigate the diagnostic and prognostic performance of VCS markers of neutrophils in our local hospital emergency setting. Methods It was an observational analytical study with 41 cases of sepsis and 43 healthy controls aged above 18 yr. Individuals with acute coronary syndrome and individuals with already diagnosed Human Immunodeficiency Virus (HIV) infection were excluded from the study. Results The mean neutrophil volume (MNV) values were not significantly different between cases and controls (P 0.138) whereas mean neutrophil conductance (MNC) and mean neutrophil scatter (MNS) measurements were significantly higher among cases as compared to controls (both P-values <0.001). According to Receiver Operating Characteristics (ROCs) curve analysis, MNV in our study failed to show statistically significant discriminatory ability in sepsis (AUC 0.54) whereas MNC (AUC 0.98) and MNS (AUC 0.95) showed marked discriminatory ability in diagnosing sepsis in this study cohort. Interpretation & conclusions Among VCS parameters, MNV failed as a standalone biomarker of sepsis in routine emergency setting whereas MNC and MNS had statistically significant diagnostic and discriminatory accuracies among hospitalized affected individuals with sepsis.

Monitoring small-scale bioreactor studies for media development using polarized total synchronous fluorescence spectroscopy (pTSFS) and synchronous light scattering (SyLS)

Biopharmaceutical process development often involves the use of small-scale bioreactors (SSBR) for optimizing media formulations and process conditions during scale up to commercial scale production. Two key process parameters (CPP) used in SSBR studies are protein titre and viable cell density (VCD). Here, we explore the efficacy of parallel polarized total synchronous fluorescence spectroscopy (TSFS||) and Synchronous Light Scattering (SyLS||) to qualitatively monitor these CPPs and quantitatively predict titre and VCD for a large-scale cell culture media optimization SSBR study. The study involved 71 different media formulations (50+ components each), and the bioprocess was run for 13 days or more. Samples were extracted at set times (Day 0, 3, 9, and 13) and clarified by centrifugation. TSFS|| spectra showed significant emission changes along with increased light scatter over the course of the bioprocess. SyLS|| measurements strongly correlated with particle size data obtained from Dynamic Light Scattering but did not correlate well with VCD probably because of the centrifugation-based sample preparation. Statistical and principal component analysis (PCA) of the pTSFS data showed that spectral variation was greater between media formulations than due to the evolving bioprocess. This prevented the development of accurate global prediction models for media performance (e.g., predicting product titre at day 9 from media spectra measured at day 0). However, classification methods were successfully used to select media subsets with better quantitative prediction accuracy based on spectral similarities. A practical binary (high/low performance) classification model based on Support Vector Machines was generated for media formulation screening. Combining emission and scatter measurements with multivariate data analysis provides a more holistic, multi-attribute bioprocess monitoring method that minimizes the need to use different offline analytical methods. This methodology can be used to monitor process trajectories and deviations, and ultimately be used to predict bioprocess CPPs when implemented on production scale processes where there is much less compositional variation in the media. We believe this SSBR-pTSFS/SyLS approach will provide a valuable resource to develop the design/parameter space for in-process monitoring at production scale from early-stage process/media development studies.

A-153 Study of the evaluation of fragment flag and percentage schistocyte count in MINDRAY® BC-6800 PLUS in a public pediatric hospital in Argentina

Abstract Background Schistocytes are fragmented red blood cells (FRCs) that arise from the mechanical destruction of red blood cells (RBCs). They indicate the possible presence of a thrombotic microangiopathy (TMA). TMA includes hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura(TTP). ICSH defined schistocytes as 4 well stablish shapes always smaller than intact RBCs. The gold standard for the identification of schistocytes is microscopic evaluation. A schistocyte count should be considered clinically meaningful if it represent the main morphological RBCs abnormality in the periferic blood smear (PBS). The results should be expressed as a percentage, after counting at least 1000 RBCs in optimal areas of the PBS. The cut off for TMA was previously stablished in above 1% (adult population) and a higher percentaje in pre term newborns (above 5%). Some blood cell count analyzers have a flag for FRC, and some of them also provide methods for an automated FRC count, through the measurement of forward scatter and the intensity of fluorescence or 2-dimensional optical analysis. The Mindray® BC 6800 PLUS provides a flag for fragments and the research FRC% parameter that quantifies fragmented RBCs. When the flag and the FRC% are combined, can bring value to diagnostic TMA. Objetives Evaluate the sensitivity and specificity of the fragment flag to predict schistocyte on the PBS of pediatric samples. Evaluate the utility of FRC% as TMA diagnostic tool and compare with the gold standard. Methods 725 pediatric samples were analyzed (0-18 years).16 of them was patient samples diagnosed with TMA. All blood cell counts were determined using Mindray®BC-6800 PLUS. Cut off 1% was considered for FRC% in TMA. Inclusion criteria for TMA samples include thrombocytopenia, increased lactate dehydrogenase and decreased hemoglobin concentration. Schistocyte counting was carried out from May-Grunwald-Giemsa-stained PBS according to the recommendations proposed by ICSH. Only ICSH established shapes were kept as valid. Results Regarding the sensitivity of the fragment flag, it was 84.62% with a specificity of 91.13% to detect the presence of schistocytes in PBS samples. In the Bland-Altman analysis, the FRC% shows a difference with a tendency to underestimation with respect to the observation on the PBS, which was less at lower percentages of schistocytes. The sensitivity of the FRC% to TMA was 75% while the specificity was 94.36% and a negative predictive value (NPV) of 99.41%. Conclusions Fragments flag represents a potentially powerful tool for screening of squistocytes in pediatric samples. Although sensibility and NPV for FRC% authomatical counts were acceptable, a few false-negative samples were found. Presence of FRC% below a threshold of 1% could exclude TMA.However PBS microscopic observation is still the gold standard to determine squistocytes presence. For the purpose of complete our study and to get further larger scale prospective data, we started collecting all data for all samples entering the laboratory in order to improve an authomatical method that should have a more rapid turn-around time at lower costs.

Measuring Dwarf Galaxy Intrinsic Abundance Scatter with Mid-resolution Spectroscopic Surveys: Calibrating APOGEE Abundance Errors

The first generations of stars left their chemical fingerprints on metal-poor stars in the Milky Way and its surrounding dwarf galaxies. While instantaneous and homogeneous enrichment implies that groups of conatal stars should have the same element abundances, small amplitudes of abundance scatter are seen at fixed [Fe/H]. Measurements of intrinsic abundance scatter have been made with small high-resolution spectroscopic data sets where measurement uncertainty is small compared to this scatter. In this work, we present a method to use mid-resolution survey data, which have larger errors, to make this measurement. Using APOGEE Data Release 17, we calculate the intrinsic scatter of Al, O, Mg, Si, Ti, Ni, and Mn relative to Fe for 333 metal-poor stars across six classical dwarf galaxies around the Milky Way, and 1604 stars across 19 globular clusters (GCs). We calibrate the reported abundance errors in bins of signal-to-noise ratio and [Fe/H] using a high-fidelity halo data set. Applying these calibrated errors to the APOGEE data, we find small amplitudes of average intrinsic abundance scatter in dwarf galaxies ranging from 0.03 to 0.09 dex, with a median value of 0.047 dex. For the GCs, we find intrinsic scatters ranging from 0.01 to 0.11 dex, with particularly high scatter for Al and O. Our measurements of intrinsic abundance scatter place important upper bounds, which are limited by our calibration, on the intrinsic scatter in these systems, as well as constraints on their underlying star formation history and mixing that we can look to simulations to interpret.

Quadrant darkfield (QDF) for label-free imaging of intracellular puncta.

Measuring changes in cellular structure and organelles is crucial for understanding disease progression and cellular responses to treatments. A label-free imaging method can aid in advancing biomedical research and therapeutic strategies. This study introduces a computational darkfield imaging approach named quadrant darkfield (QDF) to separate smaller cellular features from large structures, enabling label-free imaging of cell organelles and structures in living cells. Using a programmable LED array as illumination source, we vary the direction of illumination to encode additional information about the feature size within cells. This is possible due to the varying level of directional scattering produced by features based on their sizes relative to the wavelength of light used. QDF successfully resolved small cellular features without interference from larger structures. QDF signal is more consistent during cell shape changes than traditional darkfield. QDF signals correlate with flow cytometry side scatter measurements, effectively differentiating cells by organelle content. QDF imaging enhances the study of subcellular structures in living cells, offering improved quantification of organelle content compared to darkfield without labels. This method can be simultaneously performed with other techniques such as quantitative phase imaging to generate a multidimensional picture of living cells in real-time.

Influence of anatomical structure on antiscatter grid performance in 2D: application to x-ray angiography and a prototype 29:1 ratio grid

Objective. The use of uniform phantoms to assess the influence of x-ray scatter and antiscatter grids on x-ray angiography and fluoroscopy image quality disregards the influence of spatially variable x-ray attenuation of patients. The purpose of this work was to measure scatter to primary ratio (SPR) and antiscatter grid SNR improvement factor (K SNR) using experimental conditions which better mimic patient imaging conditions. Approach. Three adult-sized anthropomorphic phantoms were used. AP and lateral projection images of the thorax and abdomen were acquired with and without an antiscatter grid. Grids with ratio 15:1 and 29:1 (r15, r29) and x-ray fields of view 20, 25 (thorax) and 32, 42 cm (abdomen) were tested. Combined with a-priori measurements of grid scatter and primary transmission fractions, these images were used to calculate 2D SPR and K SNR maps. Main results. Results demonstrated that measurements by uniform phantom do not describe the complex 2D SPR and K SNR distributions associated with anthropomorphic phantoms. The regions of the images with the lowest primary x-ray intensity (greatest attenuation) had the highest SPR and the highest K SNR attributable to the grids. Considering all conditions, the 95th percentile of the SPR maps was in the range 42%–185% greater than the median values and that of the K SNR maps was 4%–20% higher than the median values. The combined influences of SID 120 vs. 107 cm and r29 vs. r15 grid resulted in K SNR in the range 1.05–1.49. Significance. Performance of anti-scatter grids using anatomically complex phantoms highlights the substantial variation of SPR and K SNR within 2D images. Also, this work demonstrates the benefit of the prototype r29 grid for thoracic and abdominal angiography imaging conditions is substantial, especially for large patients and radiodense image regions.

An investigation of internal scatter during computed tomography using an anthropomorphic phantom

BackgroundThe general practice for all radiographic procedures is to use Lead Shielding to optimise radiation dose to the patient. The American Association of Physicists (AAPM) in Medicine in 2019 however, made a recommendation on the use of gonad and foetal shielding. The authors have noticed that very few papers on this topic come from developing countries, hence this study embarked on. The aim of our study was to evaluate internal scatter with Lead and without Lead Shielding in an anthropomorphic phantom during Computed Tomography of the brain, chest, abdomen, and pelvis.MethodsThe methodology was based on examinations of a RANDO phantom brain, chest, abdomen and pelvis on a General Electric Optima 660 scanner; which had a 128-channel multidetector row. Examinations were performed with Lead and without Lead Shielding equivalence of 0.35 mm, and the internal scatter measured using Thermoluminescent Dosimeters. The collected data was analysed descriptively to determine the mean and standard deviation. The T-tests and two-way analysis of variance (ANOVA) were used to compare the means.ResultsThe findings of this study revealed that internal scatter was highest closest to the exposed area with higher internal scatter observed for thicker areas of the phantom. Although slightly higher readings were recorded without Pb shielding, a non-statistical significance was observed for all internal scatter measurements regardless of whether Lead Shielding was used or not.ConclusionsA non-statistical significance for Computed Tomography examinations with and without Lead Shielding in confirmation with the AAPM positional statement.

Метод эффективных вычитаний: работа с данными Иркутского радара некогерентного рассеяния

For incoherent scatter measurements, the effective subtraction technique is to alternate the duration of amplitude-modulated signals between a pair of consequently radiated pulses. The resulting gain of spatial resolution enables us to steadily assess the electron density profile by the Faraday rotation method. The paper describes the electron density measurement technique, which involves analyzing narrow-band signals from Irkutsk Incoherent Scatter Radar, and proposes an automated method of determining the electron density for the problem in which the convolution of the radiated signal waveform with backscatter signal cannot be neglected. The inverse problem of electron density recovery is considered as a standard nonlinear optimization problem, which is solved using the algorithms for global and local optimization applied consequently. We compare the electron density profiles obtained by analyzing different pulse waveforms and from Irkutsk ionosonde data.

Effective subtraction technique: implementation for Irkutsk Incoherent Scatter Radar

For incoherent scatter measurements, the effective subtraction technique is to alternate the duration of amplitude-modulated signals between a pair of consequently radiated pulses. The resulting gain of spatial resolution enables us to steadily assess the electron density profile by the Faraday rotation method. The paper describes the electron density measurement technique, which involves analyzing narrow-band signals from Irkutsk Incoherent Scatter Radar, and proposes an automated method of determining the electron density for the problem in which the convolution of the radiated signal waveform with backscatter signal cannot be neglected. The inverse problem of electron density recovery is considered as a standard nonlinear optimization problem, which is solved using the algorithms for global and local optimization applied consequently. We compare the electron density profiles obtained by analyzing different pulse waveforms and from Irkutsk ionosonde data.

Investigating Spatial and Temporal Structuring of E‐Region Coherent Scattering Regions Over Northern Norway

AbstractRecently, it has been shown that the Spread Spectrum Interferometric Multistatic meteor radar Observing Network radar system located in northern Norway is capable of measuring ionospheric E‐region coherent scatter with spatial and temporal resolutions on the order of 1.5 km and 2 s, respectively. Four different events from June and July of 2022 are examined in the present study, where the coherent scatter measurements are used as a tracer for large‐scale ionospheric phenomena such as plasma density enhancements and ionospheric electric fields. By applying a two‐dimensional Fourier analysis to range‐time‐intensity data, we perform a multi‐scale spatial and temporal investigation to determine the change in range over time of large‐scale ionospheric structures (&gt;3 km) which are compared with line‐of‐sight velocities of the small scale structures (∼5 m) determined from the Doppler shift of the coherent scatter. The spectral characteristics of the large‐scale structures are also investigated and logarithmic spectral slopes for scale sizes of 100–10 km were found to be between −3.0 and −1.5. This agrees with much of the previous work on the spatial spectra scaling for ionospheric electric fields. This analysis aids in characterizing the source of the plasma turbulence and provides crucial information about how energy is redistributed from large to small scales in the E‐region ionosphere.

Comprehensive Beam Modeling, Secondary Dose Computations and Treatment Planning for a 1.5 T MRgRT System

Magnetic resonance imaging guided radiotherapy (MRgRT) combines soft tissue visualization with online adaptive radiotherapy. The MRgRT workflow is characterized by many steps which needs to be performed swiftly and therefore greatly benefits from automation. The purpose of this study was to model a MRgRT system for dose computation and intensity modulated radiotherapy (IMRT) planning within a 1.5 T magnetic field in an independent treatment planning system (iTPS) with automation functionalities. A beam model for a 1.5 T MRgRT system was commissioned in an iTPS using beam profiles and output factor measurements. The cryostat of the MRgRT system was modeled using a cylindrical multi-layer combination of epoxy and aluminum and matched to source-to-surface dependent cryostat scatter measurement in air, and corrected for angular dependent cryostat transmission readings. The beam model was used with a fast Monte Carlo dose engine to account for the electron return and electron streaming effects within an external magnetic field. The multi-leaf collimator (MLC) was characterized with a novel synchronous and asynchronous sweeping gap (aSG) test. The aSGs have a shift (between 0 - 40 mm) between adjacent leaves which remains constant over the sweep. Measured doses map the shadowing due to the tongue-and-groove (TG) exposure and TG and leaf tip (LT) parameters are derived to match this within the iTPS. Clinical IMRT plans of 10 prostate, 5 lymph node and 5 rectal plans created in the default clinical TPS were recomputed in the iTPS and evaluated with the corresponding plan-specific quality assurance measurements. In addition, three IMRT plans were optimized within the iTPS, delivered with the MRgRT system and evaluated using PSQA using a 3%/3mm gamma criterium with a 10% threshold. Monte Carlo dose computation in the iTPS closely resembled the measured profiles in the presence of the 1.5 T magnetic field. The cryostat scatter simulations were <0.7% compared to the measurements and literature. The aSG/SGs test demonstrated that the TG width gradually increased up to 1 mm for a distance of 15 mm away from the leaf tip end. These effects were closely replicated in the iTPS with a difference of <1.5% for 95% of the aSGs. The difference between the clinical dose and the dose calculation in the iTPS was, on average, <0.5% within the volume receiving 50% of the maximum dose. The gamma pass rate was 100% for 98% of the PSQA results. The plans optimized within the iTPS were successfully delivered at the MRgRT system. We successfully modeled and commissioned a 1.5 T MRgRT system in an independent TPS. The beam model can be used for a second opinion dose computation in a magnetic field and for direct treatment planning. Next, the beam model will be incorporated in a semi-automated and adaptive workflow using the iTPS.

A long path-length optical property measurement device for highly transparent detector media

Measurements of the optical properties of highly transparent liquids are necessary for large-scale detectors that seek to detect optical signatures in kiloton-scale or larger volumes. Detailed understanding of the attenuation and scattering properties of fill media is critical to the ability to accurately simulate detector performance, and yet is often unavailable until in-situ measurements can be performed. Moreover, in-situ measurements may rely on effective attenuation lengths or other approximations to characterize performance, rather than absolute measurements of attenuation and scattering. To better understand the optical properties of potential fill media and purification schemes, a system has been developed which provides simultaneous attenuation and differential scattering cross-section measurements for highly transparent liquids. This horizontal, adjustable path-length “long-arm” system provides excellent isolation from the atmosphere, a high degree of vibration insensitivity, and a simple and reliable method of calibration. Further, scattering measurements can be carried out simultaneously at ports along the beamline equipped with optics allowing the selection of scatter angle and polarization. This enables both reliable quantitative scatter measurements and phase-function separation. This system has been extensively tested using deionized (DI) water as a benchmark, and has demonstrated repeatable measurements of attenuation lengths exceeding 100 m and scattering lengths approaching 1 km.

Radiation dose to staff from scatter radiation in the post-anaesthetic recovery ward

IntroductionThough recovery is a significant aspect of the post-surgical orthopaedic patient pathway, radiation dose from medical imaging to staff within the post-anaesthetic recovery unit, is not extensively researched. This study aimed to quantify the distribution of scatter radiation for common post-surgical orthopaedic examinations. MethodsA Raysafe Xi survey meter was used to record scattered dose at various locations around an anthropomorphic phantom, with positions simulating the potential positions of nearby staff and patients. X-ray projections of the AP Pelvis, Lateral Hip, AP and lateral knee were simulated using a portable x-ray machine. Readings were tabulated and diagrams drawn representing the distribution of scatter measurements from each of the four procedures. ResultsMagnitude of dose was dependent on imaging parameters (ie. kVp and mAs), the area of body exposed (ie. hip or knee), and the type of projection (ie. AP or lateral). Knee exposures proved much lower than hip exposures at any distance from the radiation source. ConclusionMaintaining a two-metre distance from the x-ray source was justified most profoundly by the hip exposures. Staff should have confidence that occupational limits will not be reached with adherence to the practices suggested. This study provides comprehensive diagrams and dose measurements with the aim of educating staff working around radiation.

Radiation dose to staff from medical X-ray scatter in the orthopaedic theatre

PurposeGiven the growing demand for intraoperative imaging, there is increased concern for radiation dose for orthopaedic surgical staff. This study sought to determine the distribution of scatter radiation from fluoroscopic imaging in the orthopaedic surgical environment, with particular emphasis on the positions of personnel and the type of orthopaedic surgery performed.MethodsA radiation survey detector was deployed at various angles and distances around an anthropomorphic phantom. The scatter dose rate in microsieverts per hour (µSv/h) was recorded using consistent exposure parameters for five common surgical procedures. A C-arm unit produced radiation for the hip arthroscopy, hip replacement and knee simulations, whilst a mini C-arm unit produced fluoroscopy for the foot and hand simulations.ResultsReadings were tabulated, and coloured heatmaps were generated from scatter measurements for each of the five procedures. Positions corresponding to the typical location of the surgical staff (surgeon, surgical assistant, anaesthetist, instrument (scrub) nurse, circulation (scout) nurse and anaesthetic nurse) were superimposed on heatmaps. The surgeon’s proximity to the radiation source meant this position experienced the greatest amount of radiation in all five surgical procedures. Mini C-arm doses were considered low in all procedures for positions, with and without lead protection.ConclusionThis investigation demonstrated the distribution of scattered radiation dose experienced at different positions within the orthopaedic surgical theatre. It reinforces the importance of staff increasing their distance from the primary beam where possible, reducing exposure time and increasing shielding with lead protection.

Effect of Violet Light-Filtering and Manufacturing Improvements in an Extended Depth-of-Focus Intraocular Lens on Visual Performance.

To assess the experimental visual performance and dysphotopsia characteristics of the new Tecnis Symfony OptiBlue extended-depth-of-focus with violet light-filtering (ZXR00V) intraocular lens (IOL) compared with the colorless Tecnis Symfony (ZXR00) IOL. Range of vision was assessed with simulated visual acuity defocus curves, predicted by white light through focus modulation transfer function (MTF) measurements. The clinical visual acuity defocus curve of the ZXR00 IOL was used to validate the predicted range of vision. Image quality was compared by measuring white light MTF at a spatial frequency of 15 cycles per degree (c/deg) for 3 mm and 5 mm pupil diameters with optical powers of 5 D, 20 D, and 34 D using the average corneal eye (ACE) model with the average spherical and chromatic aberration of the cataract population. Effects on dysphotopsias were predicted by measurement and computer simulation of light scatter (straylight parameter) and subsequent determination of retinal veiling luminance (RVL) in vitro. Contrast enhancement under challenging light conditions was calculated based on the effects in RVL. The simulated visual acuity defocus curves and image quality outcomes were comparable between the ZXR00V and ZXR00 IOLs. The area under the straylight curve for the straylight parameter showed a 19% improvement in halo performance with ZXR00V versus ZXR00. A 12% to 17% reduction in RVL was achieved in favor of ZXR00V over ZXR00, which enhanced contrast vision by 9% to 13% under challenging light conditions. The violet light-filtering technology and improved manufacturing of ZXR00V delivers a comparable range of vision and tolerance to refractive error to ZXR00 while mitigating dysphotopsias and enhancing contrast vision.

Assessment of the error budget for stratospheric ozone profiles retrieved from OMPS limb scatter measurements

Abstract. This study presents an error budget assessment for the ozone profiles retrieved at the University of Bremen through limb observations of the Ozone Mapper and Profiler Suite – Limb Profiler Suomi National Polar-orbiting Partnership (OMPS-LP SNPP) satellite instrument. The error characteristics are presented in a form that aims at being compliant with the recommendations and the standardizing effort of the Towards Unified Error Reporting (TUNER) project. Besides the retrieval noise, contributions from retrieval parameters are extensively discussed and quantified by using synthetic retrievals performed with the SCIATRAN radiative transfer model. For this investigation, a representative set of OMPS-LP measurements is selected to provide a reliable estimation of the uncertainties as a function of latitude and season. Errors originating from model approximations and spectroscopic data are also taken into account and found to be non-negligible. The choice of the ozone cross section is found to be relevant, as expected. Overall, we classify the estimated errors as random or systematic and investigate correlations between errors from different sources. After summing up the relevant error components, we present an estimate of the total random uncertainty on the retrieved ozone profiles, which is found to be in the 5 %–30 % range in the lower stratosphere, 3 %–5 % in the middle stratosphere, and 5 %–7 % at upper altitudes. The systematic uncertainty is mainly due to cloud contamination and model errors in the lower stratosphere and due to the retrieval bias at higher altitudes. The corresponding total bias exceeds 5 % only above 50 km and below 20 km. After computing the estimate of the overall random and systematic error components, we also provide an ex-post assessment of the uncertainties using self-collocated OMPS-LP observations and collocated Microwave Limb Sounder (MLS) data in a χ2 fashion.

Comparative analysis of patient scatter to phantom scatter for computed tomography systems.

Current computed tomography (CT) shielding practices are largely based on calculations of scattered radiation emitted from an acrylic head or body phantom, such as the Computed Tomography Dose Index (CTDI) phantom, or anthropomorphic phantoms of these two anatomical categories. This report considers the difference in scattered air kerma or dose from phantom models, to actual patient scatter under a variety of clinical scan conditions. Empirical patient scatter measurements recorded at different positions around the gantry, for 3 different CT scanners, resulted in average patient scatter fractions per unit Dose Length Product (DLP: mGycm) at 1m from the isocentre of 0.09 ± 0.03 and 0.17 ± 0.04 µGy (mGycm)-1 for head and body scans respectively. For the purposes of shielding design and scattered dose estimates to staff it is recommended that a single standard deviation be applied to these averages in the continued interest of conservatism. These values are reasonably comparative to the widely published scatter fractions by the National Council of Radiological Protection using the CTDI phantom, and the British Institute of Radiology using head and body anthropomorphic phantoms.

Analysis of limited coverage effects on areal density measurements in inertial confinement fusion implosions

Accurate diagnosis of areal density (ρR) is critical for the inference of performance metrics in inertial confinement fusion implosions. One potential source of error in this diagnosis is the existence of low mode perturbations in the imploding target, which lead to asymmetries in the inference of the ρR from different lines of sight. Here, the error accrued as a result of limited coverage of the sphere due to a finite number of detectors is quantified, and the development of a forward scatter measurement from the OMEGA neutron time-of-flight detectors is motivated. A method by which the 1D-equivalent 4π-averaged ⟨ρR⟩ can be reconstructed, if accurate mode information can be diagnosed by other means, is validated.

Abstract 5327: Characterization of a green, temperature activated nano formulation that drives the mechanism of doxorubicin toxicity from apoptosis to ferroptosis

Abstract Introduction: Nano formulations continue to be a very attractive venue for the development of “smart” drug delivery systems. Emerging green (organic) formulations do not require harsh chemical solvents and are easy to prepare. Solid Core Lipid Formulations: The lipid phase (l.p.) contained a solid fatty acid (lauric acid) and liquid lipid (oleic acid) with or without Doxorubicin. 55° surfactant containing an emulsifying agent (Brij-58) mixed with nonionic surfactant (Span 80) was dropwise added. The emulsion was cooled to form temperature-activated nanoparticles (TAN). Before usage, the emulsion was activated by heating to 41°C. Experiments: B16F10 melanoma, U87-MR glioblastoma, MDA-MB-231 and MCF-7 breast cancer cell lines were analyzed on a variety of quantitative Imaging platforms. Results iCyte (CompuCyte): Laser based instrument for obtaining quantitative fluorescence and light scatter measurements. We obtained single endpoint dosage response curves to compare the TAN with free Dox, and found the TANs were effective at 4-6 times lower equivalent Dox concentrations. Holomonitor (Phase Holographic Imaging): The HM4 resides in tissue culture incubators and allows long-term medium resolution holographic imaging. It offersa complete image processing library including routines for proliferation and cell tracking. We devised methods for 4-dimensional imaging (X pos. vs. Y pos. vs Time, vs. Density). Thus, we are able to monitor the temporal effects of the compounds. Cell Explorer (Nanolive): The Cell Explorer has a a rotating mirror that directs the laser in an orbit around the sample to obtain super-resolution 3D tomographs. It enables long-term time-lapse imaging via stage-top environmental chamber. It is well suited for quantifying sub cellular components such as mitochondria and lipids. HT-2 (Tomocube): The HT-2 is a label-free ultra-high resolution holotomography system complemented by fluorescence capabilities. Combined RI and fluorescence images give the highest information content of any of the platforms. Discussion: In 2012, Dixon et al described ferroptosis as a distinct new form of cell death through the iron dependent accumulation of oxidatively damaged phospholipids. They state that a diagnostic feature is mitochondrial shrinkage and collapse. We confirmed this in our tomographic imaging images. Other features that were detected and consistent with ferroptosis are loss of nuclear contents, a thickening of the nuclear membrane, and peri-nuclear mitochondrial accumulation. In 48-hour HM4 plots control cells show numerous mitotic events. Dox treated cells show cellular enlargement but no mitotic events. In the TAN population at about 20 hours, there is sudden death occurring in the entire population. Conclusion: Our experiments suggest a high potency of the newly developed TANs. Ferroptosis is the most likely mechanism of action. Citation Format: Farzana Parveen, Nina Filipczak, Asadullah Madni, Vladimir P. Torchilin, Ed Luther. Characterization of a green, temperature activated nano formulation that drives the mechanism of doxorubicin toxicity from apoptosis to ferroptosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5327.

Instantaneous flame front identification by Mie scattering vs. OH PLIF in low turbulence Bunsen flame

Simultaneous OH-PLIF and Mie scatter imaging were used to investigate turbulent premixed flame edge detection under a range of turbulence characteristics on a stabilised piloted Bunsen burner. A 527 nm wavelength laser beam is used to generate a Mie scattering sheet at 500 Hz, and a 283 nm wavelength laser sheet is created using 355 nm wavelength laser to pump an optical parametric oscillator to induce florescence from OH radicals at 5 Hz. A phase-locking technique is applied to synchronize and lock the two laser systems. The number density method has been used to detect flame edges in Mie scattering images, and three algorithms were applied to OH-PLIF images as a reference. A comparison of the methods and different parameter setting is made by using the metrics of location difference, flame surface density and curvature of flame edges. The processed data show that once a well-tuned window size is determined by applying the number density method, averaged spatial differences between Mie scattering images and OH-PLIF images are of the order of or smaller than the laminar flame thickness, demonstrating that under these conditions, high frequency Mie scatter measurements can be used as well as OH-PLIF images to define the flame edge at that spatial resolution. The positive result confirms that double-frame Mie scattering allows the measurement of high frequency conditional velocity distributions and flame properties simultaneously using solely Mie scattering, provided that the particle density is suitably designed to be around 16 px^2 per particle.Graphic