We first briefly review the adventure of scale invariance in physics, from Galileo Galilei, Weyl, Einstein, and Feynman to the revival by Dirac (1973) and Canuto et al. (1977). In the way that the geometry of space–time can be described by the coefficients gμν, a gauging condition given by a scale factor λ(xμ) is needed to express the scaling. In general relativity (GR), λ=1. The “Large Number Hypothesis” was taken by Dirac and by Canuto et al. to fix λ. The condition that the macroscopic empty space is scale-invariant was further preferred (Maeder 2017a), the resulting gauge is also supported by an action principle. Cosmological equations and a modified Newton equation were then derived. In short, except in extremely low density regions, the scale-invariant effects are largely dominated by Newtonian effects. However, their cumulative effects may still play a significant role in cosmic evolution. The theory contains no “adjustment parameter”. In this work, we gather concrete observational evidence that scale-invariant effects are present and measurable in astronomical objects spanning a vast range of masses (0.5 M⊙< M <1014M⊙) and an equally impressive range of spatial scales (0.01 pc < r < 1 Gpc). Scale invariance accounts for the observed excess in velocity in galaxy clusters with respect to the visible mass, the relatively flat/small slope of rotation curves in local galaxies, the observed steep rotation curves of high-redshift galaxies, and the excess of velocity in wide binary stars with separations above 3000 kau found in Gaia DR3. Last but not least, we investigate the effect of scale invariance on gravitational lensing. We show that scale invariance does not affect the geodesics of light rays as they pass in the vicinity of a massive galaxy. However, scale-invariant effects do change the inferred mass-to-light ratio of lens galaxies as compared to GR. As a result, the discrepancies seen in GR between the total lensing mass of galaxies and their stellar mass from photometry may be accounted for. This holds true both for lenses at high redshift like JWST-ER1 and at low redshift like in the SLACS sample. Of note is that none of the above observational tests require dark matter or any adjustable parameter to tweak the theory at any given mass or spatial scale.