Sargassum Sp Research Articles

Sensitivity and Acclimation of Three Canopy-Forming Seaweeds to UVB Radiation and Warming.

Canopy-forming seaweeds, as primary producers and foundation species, provide key ecological services. Their responses to multiple stressors associated with climate change could therefore have important knock-on effects on the functioning of coastal ecosystems. We examined interactive effects of UVB radiation and warming on juveniles of three habitat-forming subtidal seaweeds from Western Australia–Ecklonia radiata, Scytothalia dorycarpa and Sargassum sp. Fronds were incubated for 14 days at 16–30°C with or without UVB radiation and growth, health status, photosynthetic performance, and light absorbance measured. Furthermore, we used empirical models from the metabolic theory of ecology to evaluate the sensitivity of these important seaweeds to ocean warming. Results indicated that responses to UVB and warming were species specific, with Sargassum showing highest tolerance to a broad range of temperatures. Scytothalia was most sensitive to elevated temperature based on the reduced maximum quantum yields of PSII; however, Ecklonia was most sensitive, according to the comparison of activation energy calculated from Arrhenius’ model. UVB radiation caused reduction in the growth, physiological responses and thallus health in all three species. Our findings indicate that Scytothalia was capable of acclimating in response to UVB and increasing its light absorption efficiency in the UV bands, probably by up-regulating synthesis of photoprotective compounds. The other two species did not acclimate over the two weeks of exposure to UVB. Overall, UVB and warming would severely inhibit the growth and photosynthesis of these canopy-forming seaweeds and decrease their coverage. Differences in the sensitivity and acclimation of major seaweed species to temperature and UVB may alter the balance between species in future seaweed communities under climate change.

Pengaruh Pemberian Ekstrak Sargassum sp. dengan Pelarut Metanol pada Pakan terhadap Jumlah Eritrosit dan Differensial Leukosit Ikan Lele Dumbo (Clarias gariepinus) <Br><I>[Effect Of Extract Sargassum sp. with Methanol Solvent in Feed on Erythrocythes and Differential Leucocythes of African Catfish (Clarias gariepinus) ]<I>

Abstract The main obstacles in successful production is the attack of a disease. Preventive measures in reducing disease one of them is by means an increase in immunity the body of fish use natural compound (Parelberg et al., 2005). One of the natural compound that can be used as obtaining the immune system fish are the vitamin C, iodine, tannin (Bachtiar, dkk., 2012) and polysaccharides and derivatives as fucoidan. (Castro et. al., 2006) which is in Sargassum sp. With the provision of extract Sargassum sp. in feed is expected to optimize the number of erythrocytes and differensial leukocytes (number of neutrophils , monocytes and lymphocytes) at african catfish (Clarias gariepinus) so that it may optimize immunity the body of fish (Brown, 2000). The result of the observation of the blood of fish test is the use of extract Sargassum sp. wich get mixed in feed with doses 5, 10 and 15 g/kilogram feed during 21 days impact on the number of erythrocytes and prosentase lymphocytes blood catfishes. In doses C (15 g extract/ kilogram feed) put a value erythrocytes highest in day 21 namely 16.84 ± 1.56 x 105 cells/mm3, and give prosentase lymphocytes highest in day 21 than those wich others are 86.00 ± 3.81 %. But in a post test, B and C not seen the real difference between treatment. While for statistics the addition of extract not indicates the influence of real to the percentage neutrophils and monocytes of the blood fish. So that it can be said that addition extract Sargassum sp. with doses 15 g extract/kilogram feed give the effectiveness of the optimal response immune system than second doses other.

Investigation of Marine-Derived Fungal Diversity and Their Exploitable Biological Activities.

Marine fungi are potential producers of bioactive compounds that may have pharmacological and medicinal applications. Fungi were cultured from marine brown algae and identified using multiple target genes to confirm phylogenetic placement. These target genes included the internal transcribed spacer (ITS), the nuclear large subunit (LSU), and the β-tubulin region. Various biological activities of marine-derived fungi were evaluated, including their antifungal, antioxidant and cellulolytic enzyme activities. As a result, a total of 50 fungi was isolated from the brown algae Sargassum sp. Among the 50 isolated fungi, Corollospora angusta was the dominant species in this study. The genus Arthrinium showed a relatively strong antifungal activity to all of the target plant pathogenic fungi. In particular, Arthrinium saccharicola KUC21221 showed high radical scavenging activity and the highest activities in terms of filter paper units (0.39 U/mL), endoglucanase activity (0.38 U/mL), and β-glucosidase activity (1.04 U/mL).

Removal of sulphur black dye from its aqueous solution using alginate from Sargassum sp. (Brown algae) as a coagulant

This study deals with the coagulation potential of alginate extracted from marine algae Sargassum sp. for the removal of sulphur black dye from its aqueous solution. The alginate extraction was found to be efficient (44.8%) and the extracted alginate was characterized by Fourier transform infrared spectroscopy and Scanning electron microscopy techniques. Batch experiments (coagulation test) were performed to investigate the effect of alginate dose (10‐60 mg/L), calcium dose (1‐6 g/L) and initial dye concentration (50‐250 mg/L) on dye removal. The maximum dye removal (98.2%) was achieved under the optimal conditions of the initial dye concentration 200 mg/L, Calcium dose 6 g/L, and alginate dose 30 mg/L. The kinetics study for the coagulation process was carried out and found that it suitably fits in second‐order kinetics model. The literature on application of algal alginate for various wastewater treatment methods were compared with the present study and the results obtained revealed that the alginate from Sargassum sp. can be used as an efficient coagulant for the removal of sulphur black dye. © 2015 American Institute of Chemical Engineers Environ Prog, 34: 1427–1434, 2015

Oral Administration of Alginate From A Tropical Brown Seaweed, Sargassum sp. To Enhance Non-Spesific Defense In Walking Catfish (Clarias sp.)

Alginate that is usually extracted from brown seaweeds, is a bioactive substance with immunomodulator activity. The purpose of this research was to evaluate the effect of oral administration of alginate on the non-specific immune system of walking catfish ( Clarias sp.). An experiment was conducted in a Completely Randomized Design with five treatments in triplicates. Alginate was supplemented in feed at doses of 0 (control treatment), 2, 4, 6 and 8 g/kg of feed. The walking catfish was fed twice daily at feeding rate of 5%. Non-specific defense parameters evaluated were Nitroblue Tetrazolium (NBT) activity, phagocytic activity (PA), phagocytic index (PI), leukocyte differentiation, hematocrit and leucocrit. The parameters were observed prior to be treated, 5 th , 10 th and 15 th days after treatments. Oral administration of alginate at 4 g/kg of feed increased NBT and PA activities significantly ( P <0.05) in 5 days, while alginate at a dose of 6 g/kg increased NBT activity and monocyte percentage significantly ( P <0.05) in 5 days. These results suggested that oral administration of alginate at 4 g/kg effectively increased non-specific immune system of walking catfish.

Study of cobalt (II) biosorption on Sargassum sp. by experimental design methodology

In this study, the biosorption of cobalt (II) ions on Sargassum sp., brown algae, was investigated in a batch system. The combined effects of operating parameters such as biomass treatment, initial pH, temperature, initial metal ion concentration, and biosorbent dosage on the biosorption of cobalt (II) ions on Sargassum sp. were analyzed using response surface methodology. The Mg(NO3)2 was used as a pretreatment agent for Sargassum sp. The optimum biosorption conditions were determined as Mg-treated biomass, initial pH 7.0, temperature 45 °C, biosorbent dosage 0.1 g, and initial cobalt (II) concentration 300 mg/l. At optimum biosorption conditions, the biosorption capacity of Mg-treated biomass for cobalt (II) ions was found to be 80.27 mg/g after 90-min biosorption. The Langmuir and Freundlich isotherm models were applied to the equilibrium data. The results are best fitted by the Freundlich model.

Encapsulation of Brown Seaweed Pigment by Freeze Drying: Characterization and its Stability during Storage

The general objective of this study was to evaluate the stability of encapsulated pigment prepared from brown seaweed (Sargassum sp). A simple and rapid extraction procedure was employed to collect brown–algal photosynthetic pigments, followed by encapsulation using freeze drying with maltodextrin and Tween–80 as the encapsulating agents. The product showed yellowish green powdery pigments. Chromatography analysis confirmed the presence of trans–fucoxanthin as major carotenoid. Then, the stability of pigment in the freeze–dried powder during dark storage was studied at 28°C, 45°C, and 65°C. The degradation of the surface of the encapsulated pigments was evaluated chromametrically by measuring the L*, a*, and b* values. Finally, the half–life of the encapsulated pigment was estimated.

Gastropoda (Mollusca) associated to Sargassum sp. beds in São Sebastião Channel - São Paulo, Brazil

The phytal is characterized by the formation of seaweed beds and a great diversity of associated species, the malacofauna being one of its main components. Aiming to record the species of Gastropoda associated to the brown algae Sargassum sp. C. Agardh, 1820, this study was carried out in São Sebastião Channel, northern coast of São Paulo, and nearby areas. A total of 13945 individuals were identified, belonging to 35 families and 62 species. Cerithiidae, Phasianellidae and Columbellidae were the most abundant families, represented by 34, 33 and 17% of the total collected individuals, respectively. Bittiolum varium (Pfeiffer, 1840) (Cerithiidae) and Eulithidium affine (C. B. Adams, 1850) (Phasianellidae) are the dominant species, followed by the columbelids Mitrella dichroa (G. B. Sowerby I, 1844), Anachis fenneli Radwin, 1968 and Costoanachis sertulariarium (d'Orbigny, 1839). Among the least abundant species, some of them may be considered as of fortuitous occurrences, while others seem to be typical in those habitats, although rare. The presence of juvenile specimens was recurrent, this indicating that the algae can function as a nursery for most of these species. The expressive values found, for both abundance and number of species, illustrate the great ecological importance of the phytal habitats for the gastropod species.

Optimization of biogas production from Sargassum sp. using a design of experiments to assess the co-digestion with glycerol and waste frying oil

A design of experiments was adopted to assess the optimal conditions for methane production from the macroalgae Sargassum sp. co-digested with glycerol (Gly) and waste frying oil (WFO). Three variables were tested: % total solids of algae (%TSSargassumsp.), co-substrate concentration (gGly/WFOL−1), and co-substrate type (Gly or WFO). The biochemical methane potential (BMP) of Sargassum sp. was 181±1L CH4kg−1 COD. The co-digestion with Gly and WFO increased the BMP by 56% and 46%, respectively. The methane production rate (k), showed similar behaviour as the BMP, increasing 38% and 19% with Gly and WFO, respectively. The higher BMP (283±18L CH4kg−1 COD) and k (65.9±2.1L CH4kg−1 CODd−1) was obtained in the assay with 0.5% TS and 3.0gGlyL−1. Co-digestion with glycerol or WFO is a promising process to enhance the BMP from the macroalgae Sargassum sp.

A-Amylase and a-Glucosidase Inhibition by Brown Seaweed (Sargassum sp) Extracts

Inhibition of intestinal α-amylase and α-glucosidase is an important strategy to control post-prandial hyperglycemia associated with diabetes mellitus. In vitro inhibitory effects of crude extracts of seaweed against α-amylase and α-glucosidase were studied. Crude ethyl acetate extracts of Sargassum aquifolium were the stronger inhibitor to α-amylase and α-glucosidase than others. Furthermore, Sargassum aquifolium ethyl acetate extract significantly suppressed the rise in postprandial glucose level after oral administration of glucose in normal rats. The results of this study suggest that the crude Sargassum aquifolium extract may suppress the rise in postprandial hyperglycemia in vivo in part, through inhibition of alpha amylase and glucosidase.

Antioxidant and anticoagulant activity of polyphenol and polysaccharides from fermented Sargassum sp.

The current investigation was carried out with an objective of determining the structural characteristic of polysaccharides extracted from fermented Sargassum sp. to be used as potent natural heparin substitute anticoagulant compound. Sargassum sp. fermented with marine lactic acid bacteria was initially subjected to ethanol precipitation for the recovery of bioactive compounds. Antioxidant activity was maximum in the soluble fraction whereas anticoagulant activity was observed to be high in the precipitate which correlated with the increased polyphenols and total sugars respectively. The precipitate was purified by anion exchange chromatography and the fractions collected were analyzed for total sugars and anticoagulant activity. There was 2.6–3.9-folds increase in anticoagulant activity in the final purified fractions, with a maximum activity in case of sample fermented with Enterococcus faecium (6.7±0.22IU/mg). Structural elucidation of potential anticoagulant polysaccharide by Fourier Transform Infrared Spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR) analysis indicated the presence of alginate rich in mannuronic acid.

Rekayasa Teknologi Fermentasi Limbah Rumput Laut sebagai Probiotik pada Budidaya Intensif Ikan Sistem Akuaponik &lt;br&gt;&lt;i&gt;[Engineering Technology Fermentation Waste Seaweed As Probiotics On The Cultivation Of Intensive Fish Aquaponic System]&lt;I&gt;

Abstract This research is done by doing a test fermentation technology in order to manufacture enriched media probiotic (probiotic 's) and the identification of the type and number of probiotic bacteria, and nutrient elements absorption of N and P. Results obtained shows that the number of microbes Lactobacillus sp. as probiotic bacteria may increase on media waste Gracilaria sp., Kappaphycus sp., Sargassum sp. and the levels of nitrogen and phosphor is high from fermented waste with media indicating that the fermentation kelp Gracilaria sp. waste provides the best results compared to fermentation waste Kappaphycus sp. and Sargassum sp.

Pengaruh Suhu Ekstraksi Terhadap Kualitas Natrium Alginat Rumput Laut Sargassum sp.

Sargassum sp. are the living marine resource that are potential as alginate resources. Sodium alginate is used in the food industry, and non food industry, such as for a coagulant, suspending, stabilizers, film formers, gel formation, and emulsifier. The objective of research was to know the effect of temperature exstraction toward quality of alginate, such as rendement, moisture, ashes content, and viscosity of Sargassum sp. Sampling of Sargassum sp. was done in Krakal Beach Gunung Kidul, Yogyakarta. The research method used experimental method and was design completaly Randomized Design (CRD). Samples were extracted by Na2CO3 7 % solution under diffirencees temperature from 40 oC - 80 oC, temperature range 10 °C, each treatment 3 times replicated. Correlation between extraction temperature and sodium alginate qualities were showed positive linier model regression for rendement sodium alginate, negative linier regression model for viscosity and moisture, but not significant for ash content. The result showed the highest rendement of sodium alginate was produced on temperature of 80 oC, that was 28.31% ± 0.48%. The lowest moisture was produced on temperature of 80 oC, that was 14.97 ± 1.55%. Highest viscosity was produced on temperature 40 oC, that was 27.25 ± 0.93 cPs.

Pengaruh Lama Penyinaran dan Salinitas terhadap Pertumbuhan dan Jumlah Klorofil A Sargassum sp. &lt;br&gt;&lt;i&gt;[Effect Of Photoperiod And Salinity On The Growth And Chlorophyll A From Sargassum sp. ]&lt;i&gt;

Abstract Growth of Sargassum sp. in the waters of the world is different in each of its waters, it is strongly influenced by several factors, namely salinity and photoperiod. Salinity is closely related to the osmotic pressure that effects the body's balance of aquatic organisms. Photoperiod effects directly or indirectly, in particular on the algae, namely as a source of energy for photosynthesis. Photosynthesis process will occur not only with light, but also with the help of chlorophyll. The aim of this study was to determine the effect of photoperiod and salinity on growth and chlorophyll a Sargassum sp. and to determine the interaction relationship between photoperiod and of salinity on growth and chlorophyll a Sargassum sp. The design of the study is a Completely Randomized Design (CRD) Factorial test followed by Duncan's Multiple distance. Materials used in this study is Sargassum sp. with 9 treatments and 3 replications. Data results showed that the effect of salinity was not significantly different (F calculated &lt; F table 0.05) on the growth of Sargassum sp. Effect of photoperiod did not different significant on the growth of Sargassum sp. in the first week until the third week, but different very significant (F calculated&gt; F table 0.01) in the fourth week of treatment where E is the salinity of 30 ppt with photoperiod light 16 and 8 hours dark that significantly different with A, C, D, F, and I which not significantly different with B, G, H. On growth, there is no interaction of salinity and photoperiod. On chlorophyll a there is no difference significant in treatment A, B, C, D, E, F, G, H, and I. Salinity and photoperiod had no effect on chlorophyll a Sargassum sp., and there is no interaction of salinity and photoperiod.

Screening for in vitro Cytotoxic Activity of Seaweed, Sargassum sp. Against Hep-2 and MCF-7 Cancer Cell Lines

Discovery of anticancer drugs that kill or disable tumor cells in the presence of normal cells without undue toxicity is a potential challenge for therapeutic care. Several papers in the literature have emphasized the potential implications of marine products such as seaweeds which exhibit antitumor activity. Study attempts to screen the antitumor effect of Sargassum sp, against chosen cell lines such as MCF-7 (Breast cancer) and Hep-2 (Liver Cancer). Ethanol extract of Sargassum sp. was concentrated using a Soxhlet apparatus and dissolved in DMSO. In vitro cytotoxic activity of Sargassum sp at various concentrations (100 μg/ml-300 μg/ml) screened for antitumor effect against the chosen cell lines using MTT assay (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a yellow tetrazole). The study documented that the percentage of cell viability has been reduced with increased concentration, as evidenced by cell death. Sargassum sp extract shows potential cytotoxic activity (P≤0.05) with IC50 of 200 μ g/ml and 250 μg/ml against Hep-2 and MCF-7 cell lines respectively. The ethanol fraction of Sargassum sp induced cell shrinkage, cell membrane blebbing and formation of apoptotic bodies with evidence of bioactive components as profound influencing factors for anti-tumor effects. Further research need to be explored for the successful application of Sargassum sp as a potent therapeutic tool against cancer.

KAROTENOID DARI MAKROALGAE DAN MIKROALGAE: POTENSI KESEHATAN APLIKASI DAN BIOTEKNOLOGI

SUMMARY Algae, both micro and macroalgae, is one of the largest producers of carotenoids. The major composition of carotenoid on algae are βcarotene, astaxanthin, luthein, zeaxanthin, cryptoxanthin, and fucoxanthin which have important roles for human health. Carotenoids were produced by several microalgae species such as Dunaliella sallina, Haemotococcus pluvialis, Chlorella pyrenoidosa, Spirulina platensis, Nannnochloropsis oculata, and also from some macroalgae species such as Kappaphycus alvarezii, Sargassum sp, and Caulerpa sp. Carotenoids from algae has been proven as a powerful antioxidant and may prevent some degenerative diseases, cardiovascular, and cancer. Carotenoid also has been applied as a natural dye and dietary supplements. Biotechnology has been developed to increase the production of carotenoids from micro- and macroalgae. The large-scale cultivation of microalgae, either in open or closed system are shown to increase carotenoid production. During cultivation, some stress conditions can be specifically manipulated to optimize carotenoid production from microalgae.

Phenology and alginates of two Sargassum species from the Caribbean coast of Colombia

The genus Sargassum (Phaeophyceae) forms large beds on rocky shores of the Colombian Caribbean, showing large seasonal fluctuations in biomass that are related to upwelling events in the area. This study describes the phenology of two benthic species of Sargassum from the Caribbean coast of Colombia, and presents a preliminary assessment of the alginate concentration and properties of viscosity and gel strength to determine their potential as an exploitable resource. Samples were collected during 2007 in the Santa Marta area. The first species was identified at genus level as Sargassum sp. The highest values of biomass and abundance of receptacles were recorded in April and the lowest between June and February. The other species was Sargassum cymosum. It showed higher biomass and persisted a longer time than Sargassum sp. In spite of this, the biomass and reproductive period of both species were markedly seasonal and low during most of the year. Alginate yield was relatively high in both species, compared to that reported for other Sargassum species. The viscosity of the alginates was very low and may thus have only few applications in the industry; however, the gel strength of the alginates of both species was competitive, compared with other commercial algal products. This study suggests that while these two species of Sargassum in Colombia are potential candidates for the commercial production of alginates in regard to some applications, the marked seasonality of the species advises caution for a sustainable exploitation.

Potensi Antibakteri Ekstrak Rumput Laut Terhadap Bakteri Penyakit Kulit Pseudomonas aeruginosa, Staphylococcus epidermidis, dan Micrococcus luteus

Along with the increasing resistance of bacteria in the health world, it is necessary discover a new drug. Sources of new antibacterial bioactive compounds can be obtained from seaweeds. The purposes of the research were to determine the antibacterial activity of crude extract seaweed againts the skin disease bacteria, Pseudomonas aeruginosa, Staphylococcus epidermidis and Micrococcus luteus, and to identify classes of compounds contained in crude extract of seaweed. This research was done from January to Mei 2012. Seaweeds sampling (Caulerpa sp, Eucheuma sp, Gracilaria sp dan Sargassum sp) was conducted in territorial water of Jepara. Extraction process and analysis of bioactive compound of crude extracts was conducted at Laboratory, Marine Science Department, Diponegoro University, Semarang. Test antibacterial activity was conducted at Medicine Laboratory, Ungaran. The method used in this research was experimental laboratories. Extraction process was conducted with solid-liquid extraction method. Extract were tested for antibacterial activity by agar diffusion method according to Kirby-baurer. Based on the antibacterial activity’s assay, 12 extracts which have antibacterial activity against test bacteria P. aeruginosa, S. epidermidis and M. luteus were obtained the 12 seaweed extracts the highest consentration of inhibition zone was 200 µg/disk in ethyl acetate extract of the Sargassum sp was the most active extracts against bacteria P. aeruginosa and M. luteus, while the methanol extract of Sargassum sp was the most active extracts against the bacteria S. epidermidis. Steroids was the most dominant compounds on all of 12 seaweed extracts.

Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

Fucose-containing sulfated polysaccharides can be extracted from the brown seaweed, Sargassum sp. It has been reported that fucose-rich sulfated polysaccharides from brown seaweeds exert different beneficial biological activities including anti-inflammatory, anticoagulant, and anti-viral effects. Classical extraction of fucose-containing sulfated polysaccharides from brown seaweed species typically involves extended, multiple-step, hot acid, or CaCl2 treatments, each step lasting several hours. In this work, we systematically examined the influence of acid concentration (HCl), time, and temperature on the yield of fucose-containing sulfated polysaccharides (FCSPs) in statistically designed two-step and single-step multifactorial extraction experiments. All extraction factors had significant effects on the fucose-containing sulfated polysaccharides yield, with the temperature and time exerting positive effects, and the acid concentration having a negative effect. The model defined an optimized single-step FCSPs extraction procedure for Sargassum sp. (a brown seaweed). A maximal fucose-containing sulfated polysaccharides yield of ∼7% of the Sargassum sp. dry matter was achieved by the optimal extraction procedure of: 0.03 M HCl, 90°C, 4 h. HPAEC-PAD analysis confirmed that fucose, galactose, and glucuronic acid were the major constituents of the polysaccharides obtained by the optimized method. Lower polysaccharide yield, but relatively higher fucose content was obtained with shorter extraction time. The data also revealed that classical multi-step extraction with acid ≥0.2 M HCl at elevated temperature and extended time had a detrimental effect on the FCSPs yield as this treatment apparently disrupted the structural integrity of the polymer and evidently caused degradation of the carbohydrate chains built up of fucose residues.

Pengaruh Ekstrak Alga Cokelat (Sargassum sp.) terhadap Pertumbuhan Bakteri Escherichia coli Secara In Vitro &lt;br&gt;&lt;i&gt;[Effect of Brown Algae Extract (Sargassum sp.) on Growth of Escherichia coli In Vitro]&lt;i&gt;

Abstract Algae Sargassum sp. showed the ability to inhibit the growth of a maximum of several types of pathogenic bacteria such as Escherichia coli. Because of Sargassum sp. have antibacterial with 2 active ingredients are compounds of phenol and tannin fiber. So Sargassum sp. potential to serve as antibakteri substances against several types of bacterial pathogens such as bacteria that cause diarhea.The aim of the study was to determine antibacterial activity brown algae exstract (Sargassum sp.) and determine best concentration from brown algae exstract (Sargassum sp.) to kill of E. coli. This study was conducted at several places such as Saints and Technology Faculty and Veterinary Faculty, Airlangga University Surabaya. Research was done September 2010. This study was conducted in the laboratory using experimental method completely randomized design with twelve treatments and there replications. The main objectives of this study were MBC extract of Sargassum sp. Result of the study analyzed with Khi-Khuadrat. Result of the study showed that extract Sargassum sp. has antimicrobial activity against E. coli were determined with in vitro method. Based on MBC data were found to be effective to kill of E. coli at 50 %. Exploration potential of extracts from brown algae (Sargassum sp.) against various species of bacteria that cause diarrhea are expected to become a new information about the content of extracts of Sargassum sp. as an antibacterial. Also from this research will be obtained an alternative treatment of diarrheal diseases that come from living marine resources and will eventually increase the economic value of fishery commodities which have not explored its use as Sargassum sp.