Sap Collected Research Articles

Collection methods to preserve nutritive and physicochemical properties of unfermented coconut (Cocos nucifera) sap

Quality evaluation of unfermented coconut (Cocosnucifera) sap was done using two sap collection methods of application of Hal bark (Vateria acuminate) to the clay pots of 4L (TM) and novel sap collection method (NSCM), during 12 hours (from 6pm to 6 am). The novel sap collecting device is a recent innovation by Coconut Research Institute(CRI) – Sri Lanka. Twelve tapped coconut palms (Dwraft variety, 45 years old) were used and filtered sap was stored (-18oC). Volume, pH, total soluble solids, alcohol content, total acidity, color, sugar profile, total phenols, EC50, Ascorbic acid content and mineral in two types of unfermented coconut sap samples were determined. The results revealed that coconut sap collected from NSCM has significantly high pH (5.99), moisture (83.20%), sucrose (13.71%), total sugar (19.99%) compared to the collected sap from TM. In contrast, the sap of TM method were significantly rich with total phenolic (65.90 mgGAE/100ml), EC50 (143.03 mg/ml), AEAC (0.2568 mg Vit C in 1g sample), browning index (6.76) and yellowing index (15.92). Moreover, Ca (39.3mg/L), Fe (3.08mg/L), Mn (0.96mg/L), Sr (0.14mg/L) Ba (0.33mg/L) were significantly high in TM. Hence, the novel sap collection method can be concluded as the best approach for collecting quality unfermented coconut sap with its natural quality.

The effect of tree age, daily sap volume and date of sap collection on the content of minerals and heavy metals in silver birch (Betula pendula Roth) tree sap.

The aim of this study was to determine the effect of the age of trees, daily sap volume as well as the term of tapping birch sap collected in the forest environment on the content of selected minerals (zinc, copper and manganese) and heavy metals (lead, nickel, chromium and cadmium). The study was performed on material taken from two stands (aged 34 and 84 years) in a moist broadleaved forest habitat with a dominant share of silver birch (Betula pendula Roth).The research results confirmed the presence of both nutritional essential minerals and hazardous heavy metals in the birch sap. At the same time, the content of minerals and heavy metals was found to be very variable and the differences between their concentrations, recorded on the same day of collecting in several trees of the same age group, can be even several dozen times higher. Depending on the examined elements, the factors influencing their content vary. The age of the trees determines only the manganese content; daily sap volume significantly affects the content of manganese and copper, and date of collection differentiates the content of zinc, lead, nickel and cadmium.The results may be interesting in the context of developing procedures for collecting birch sap for the purpose of obtaining raw material with beneficial nutritional values and a high level of health safety. For this reason, our recommendation for guaranteeing the health safety and high nutritional value of birch sap is to combine batches of raw material taken from as many trees as possible, and at the same time to publicize the fact that collecting birch sap from just one single tree may result in a raw material that is both dangerous and has no nutritional benefits.

DEVELOPMENT OF NIPA (Nypa fruticans) SAP CLOSED COLLECTION VESSEL

The ultimate goal of the present study was to develop a secure, safe, and hygienic nipa sap collection system for bioethanol production, with the aim of preserving its physico-chemical properties such as physical appearance, pH and sugar brix by reducing the rate of fermentation while attached to the peduncle. The developed collection system was evaluated in terms of the physical and chemical properties of nipa sap collected and ethanol yield in comparison to the traditional and existing collection system used by the nipa community which utilizes bamboo shingle as their collector. Physical appearance of the sap collected using the designed collection system had no foreign materials after harvesting while the traditional collection system had shown traces of insect infestation. The sap that was produced for both of the designed and traditional collection system was milky-white and yellowish-white in color respectively. There was a significant difference in terms of pH concentration of the sap collected using the designed collection system compared to the sap collected using the traditional system overtime. Sugar brix of nipa sap collected using designed collection system is significantly higher than the sap collected using traditional system. A total ethanol yield of 32.25% and 75.54% was obtained for the designed and traditional collection system respectively. Cost Analysis revealed that the designed collection system was found to be cheaper (PhP 11.93) than the traditional collection system (PhP. 20.00). The developed closed collection system can preserved the chemical properties of the nipa sap and could prevent acceleration of fermentation and the deterioration of its potential to yield more ethanol.

Nutritional profiling of coconut (Cocos nucifera L.) inflorescence sap collected using novel coco-sap chiller method and its value added products

Coconut inflorescence sap is a phloem sap collected from the unopened coconut spadix. A new ‘coco-sap chiller method’ (CSCM) is developed to collect fresh and unfermented sap devoid of extraneous matter. A study was conducted to identify and compare the nutrients present in the sap collected by CSCM and the sap collected by traditional method (TM) using lime coated earthen pot, and also in the sap concentrate and coconut sugar prepared from sap collected by CSCM. Profiling of amino acids, phenolic acids, flavonoids and vitamins was also done using Ultra-Performance Liquid Chromatography/ Tandem Mass Spectrometry (UPLC coupled with TQD-MS/MS). Distinct variation in physical and biochemical properties were recorded between the sap collection methods. The sap collected by CSCM was slightly alkaline (pH 7–8), golden brown in color, sweet and delicious and rich in nutrients and zero alcoholic. While the sap collected by TM was acidic (pH below 6), oyster white in color and gave astringent smell and had about 2.32% alcohol. Sap collected by CSCM was rich in essential amino acids like lysine, threonine and histidine. A total number of thirteen phenolic acids and seven flavonoids were identified from coconut sap, sap concentrate and sap sugar. Vanilic acid, syringic acid, trans-cinnamic acid and p-hydroxy benzoic acid are the major phenolic acids and catechin, hesperitin, and myricetin are the major flavonoids identified. Vitamin C, niacin, and tocopherol are the major vitamin identified. Except few vitamins, all the nutrients present in the sap were preserved in the sap concentrate and coconut sugar. In summary, coconut sap collected by CSCM was fresh and contained numerous health promoting biochemical constituents such as phenolics, flavonoids and vitamins. Hence it can be consumed as ready to serve non-alcoholic health drink. Similarly, nutrient rich sap concentrate and coconut sugar can be prepared from fresh sap collected by CSCM without the addition of any chemicals.

Raw Sap Consumption Habits and Its Association with Knowledge of Nipah Virus in Two Endemic Districts in Bangladesh.

Human Nipah virus (NiV) infection in Bangladesh is a fatal disease that can be transmitted from bats to humans who drink contaminated raw date palm sap collected overnight during the cold season. Our study aimed to understand date palm sap consumption habits of rural residents and factors associated with consumption. In November-December 2012 the field team interviewed adult respondents from randomly selected villages from Rajbari and Kushtia Districts in Bangladesh. We calculated the proportion of people who consumed raw sap and had heard about a disease from raw sap consumption. We assessed the factors associated with raw sap consumption by calculating prevalence ratios (PR) adjusted for village level clustering effects. Among the 1,777 respondents interviewed, half (50%) reported drinking raw sap during the previous sap collection season and 37% consumed raw sap at least once per month. Few respondents (5%) heard about NiV. Thirty-seven percent of respondents reported hearing about a disease transmitted through raw sap consumption, inclusive of a 10% who related it with milder illness like diarrhea, vomiting or indigestion rather than NiV. Respondents who harvested date palm trees in their household were more likely to drink sap than those who did not own date palm trees (79% vs. 65% PR 1.2, 95% CI 1.1–1.3, p<0.001). When sap was available, respondents who heard about a disease from raw sap consumption were just as likely to drink it as those who did not hear about a disease (69% vs. 67%, PR 1.0, 95% CI 0.9–1.1, p = 0.512). Respondents’ knowledge of NiV was low. They might not have properly understood the risk of NiV, and were likely to drink sap when it was available. Implementing strategies to increase awareness about the risks of NiV and protect sap from bats might reduce the risk of NiV transmission.

Xylem sap collection and extraction methodologies to determine in vivo concentrations of ABA and its bound forms by gas chromatography-mass spectrometry (GC-MS)

BackgroundAccurate quantification of xylem sap ABA concentrations is important to underpin models of root-to-shoot ABA signalling to predict the physiological effects of soil drying. Growing tomato plants in a whole plant pressure chamber allowed sequential xylem sap collection from a detached leaf, the petiole stub of an otherwise intact plant and finally the de-topped root system of the same plant, to determine the impact of xylem sap sampling methodology on xylem ABA concentration. Since xylem sap can contain bound forms of ABA, a novel gas chromatography-mass spectrometry (GC-MS) procedure was developed to chemically separate free ABA from two in planta bound ABA forms known as Adducts I and II and ABA-glucose-ester (ABA-GE).ResultsXylem sap ABA concentrations were highly dependent on the sampling methodology used: the highest concentrations were detected in sap collected by applying an overpressure to detached leaves following the measurement of leaf water potential. Irrespective of xylem sap source, the wild-type cultivars Ailsa Craig and Rheinlands Ruhm had higher free ABA concentrations than a range of ABA-deficient mutants (notabilis, flacca and sitiens). However, in the mutants, concentrations of bound forms of ABA were similar to wild-type plants, and similar to free ABA concentrations.ConclusionsAlthough xylem concentrations of these bound ABA forms and ABA-GE suggest they have a limited physiological impact on ABA homeostasis in tomato, the methods developed here will allow a more complete understanding of ABA biochemistry and root-to-shoot signalling in species known to have higher concentrations of these compounds.

고로쇠나무 수액 채취를 위한 적정 천공 크기와 천공 수

Optimum tapping size and number for sap collection were investigated to minimize damage on tree growth and secure resources for sap by sap collection of Acer mono. The amounts of sap collected by tapping size of 12 mm, 10 mm and 8 mm was 189 L, 140 L and 193 L, respectively. Fusion rates by tapping size were 100% for 8 mm, 89% for 10 mm and 85% for 12 mm, respectively. Amount of sap by size and number of tapping were much at three, two and three tappings to small, middle and large diameter tree in case of 8 mm tapping, and it was large in quantity at two tappings of 12 mm tapping to large diameter tree, respectively. Trees tapped from one to three tappings of 8 mm size for small diameter tree with treatment of DB ointment (mixture of thiophane ointment and 2% bamboo charcoal powder) were completely filled up within 6 months. Diameter growth by number of tapping of 8 mm size was 0.60 mm for one tapping, 1.12 mm for two tappings and 0.47 mm for three tappings to small, middle and large diameter tree, respectively. In case of 12 mm tapping size, diameter growth was fast in the order of large (0.55 mm), middle (0.30 mm) and small (0.23 mm) diameter tree, respectively.

Melon resistance to the aphid Aphis gossypii: behavioural analysis and chemical correlations with nitrogenous compounds

AbstractIn the melon, the Vat (monogenic, dominant) resistance gene governs both an antixenotic reaction to the melon aphid Aphis gossypii Glover (Homoptera, Aphididae) and a resistance to non‐persistent virus transmission, restricted to this vector species. We investigated the behavioural features and tissue localisation of the antixenosis resistance by the electrical penetration graph technique (EPG, DC system). We also compared the chemical composition in amino compounds and proteins of the phloem sap collected from two isogenic lines of melon (Cucumis melo L.), carrying the Vat gene or not. All behavioural and chemical data indicated that this resistance is constitutive. EPG analysis clearly showed that access to phloem, although delayed by alterations in pathway activities, was not impaired in terms of frequency of access or initiation of feeding. The most striking feature was, however, a very reduced duration of ingestion from phloem of resistant plants, making this compartment one of the tissues where the effects of the Vat gene are unambiguously expressed. This was confirmed by clear differential activity of phloem extracts in artificial no‐choice bioassays. Chemical analyses have shown that phloem saps from the two isogenic lines were extremely similar in profiles of ninhydrin positive compounds, and contained a low total amount of free amino acids (less than 10 mM). Out of more than 40 distinguishable peaks in the chromatograms (protein and non‐protein amino acids, as well as small peptides), only five differentiated the two genotypes. Two of them were increased in the resistant genotype: glutamic acid and a major unknown peak, probably a non‐protein amino acid (different from β pyrazolyl‐alanine, a Cucumis‐specific amino acid). The three others were depressed in resistant plants, and included the sulphur amino acid cystine and a peptide peak partly composed of the cysteine‐containing peptide glutathione (reduced form). Sap collection also showed that phloem exudation rates, as well as total protein and glutathione levels, were depressed in phloem sap from resistant plants. Such data are all indicative of a modified phloem‐sealing physiology, linked to sulfhydryl oxidation processes, in plants carrying the Vat gene. The originality of the mechanism of Vat resistance to aphids is discussed.

High-Vacuum Pumping Effects on Maple Sap Sugar Yield

Abstract Some maple producers are reluctant to take advantage of high-vacuum pumping to increase yields of sugar maple sap in their closed-tubing sap collection systems. They believe that only sap of significantly lower sugar content will be obtained. During 1985 and 1986, at Lake Placid, NY, sap collected from sugar maple trees subjected to three levels of vacuum pumping (10-, 15-, and 20-in. Hg) was compared to sap obtained by gravity flow. In most instances the higher levels of vacuum resulted in significantly higher sap volumes with no significant decrease in sap sugar content. Application of 15-in. Hg pumped vacuum at the taphole in a well-maintained tubing system should result in a significant increase in syrup production. North. J. Appl. For. 6:126-129, September 1989.