River Sampling Sites Research Articles

Distributions of Fecal Indicators at Aquaculture Areas in a Bay of Republic of Korea.

Aquaculture products, such as clams, scallops, and oysters, are major vectors of fecal-derived pathogens. Male-specific and somatic coliphages are strongly correlated with human noroviruses, the major enteric viruses worldwide. Geographic information system with local land-use patterns can also provide valuable information for tracking sources of fecal-derived pathogens. We examined distributions of four fecal indicator microorganisms, i.e., male-specific and somatic coliphage, total coliform, and Escherichia coli (E. coli) in three river and seawater sampling sites located on the coast of Gomso Bay in the Republic of Korea during the sampling period (from March 2015 to January 2016). Geospatial analyses of fecal indicators and correlation between environmental parameters and fecal indicators or among fecal indicators were also performed. Overall, river water samples showed highest concentrations of both types of coliphage in summer (July 2015). High concentrations of both total coliform and E. coli were detected in river water during the period from July to September 2015. High concentrations of all fecal indicators were found at site GL02, located in the innermost part of Gomso Bay, which has high-density agriculture and residential areas. Environmental factors related to precipitation-cumulative precipitation on and from 3 days before the sampling day (Prep-0 and Prep-3, respectively)-and salinity were strongly correlated with concentrations of all fecal indicators. The present results suggest that investigations of multiple fecal indicators with a systemic geospatial information are necessary for precisely tracking fecal contaminations of aquaculture products.

Pollution characterization and multi-index ecological risk assessment of microplastics in urban rivers from a Chinese megacity

Currently, a comprehensive understanding of the pollution risks of microplastics (MPs) in urban river ecosystems is still lacking. This study investigated the spatial distribution and morphological characteristics of MPs in surface waters of major rivers in Shenzhen, a megacity in China, using laser direct infrared (LDIR) imaging. A promisingly comprehensive risk assessment method, MultiMP, was first proposed in this study, taking into account the multidimensional characteristics of MPs including abundance, size, shapes, and polymer types. The results showed that MPs were widespread and highly heterogeneous, and the abundance of MPs ranged from 38 to 18380 particles/L, with an average of 2305 particles/L. Morphologically, polyamide (PA) (average 53.7 %), 30–50 µm (73.8 %), and pellet (65.7 %) were the predominant MPs types. Driving factors analyses revealed geographical distance, salinity, water temperature, and total nitrogen had relatively higher impacts on the abundance and morphology of MPs. The MultiMP results indicated that most of the river sampling sites and five major basins in Shenzhen were at moderate to high-risk levels. Polymer type and abundance had a relatively high impact on the environmental risk of MPs in the region. These findings contribute to improving the insights and management of the MPs pollution risks in megacity water bodies.

QPCR-based phytoplankton abundance and chlorophyll a: A multi-year study in twelve large freshwater rivers across the United States

Phytoplankton overgrowth, which characterizes the eutrophication or trophic status of surface water bodies, threatens ecosystems and public health. Quantitative polymerase chain reaction (qPCR) is promising for assessing the abundance and community composition of phytoplankton. However, applications of qPCR to indicate eutrophication and trophic status, especially in lotic systems, have yet to be comprehensively evaluated. For the first time, this study correlates qPCR-based phytoplankton abundance with chlorophyll a (the most widely used indicator of eutrophication and trophic status) in multiple freshwater rivers. From early summer to late fall in 2017, 2018, and 2019, we evaluated phytoplankton, chlorophyll a, pheophytin a, and the Trophic Level Index (TLI) in twelve large freshwater rivers in three regions (western, midcontinent, and eastern) in the United States. qPCR-based phytoplankton abundance had positive allometric correlations with chlorophyll a concentration (adjusted R2 = 0.5437, p-value < 0.001), pheophytin a concentration (adjusted R2 = 0.3378, p-value <0.001), and TLI (adjusted R2 = 0.4789, p-value < 0.001). Thus, a greater phytoplankton abundance suggests a higher trophic status. This work also presents the numerical values of qPCR-based phytoplankton abundance defining the boundaries among trophic statuses (e.g., oligotrophic, mesotrophic, and eutrophic) of freshwater rivers. The sampling sites in the midcontinent rivers were more eutrophic because they had significantly higher chlorophyll a concentrations, pheophytin a concentrations, and TLI values than the sites in the western and eastern rivers. The higher phytoplankton abundance at the midcontinent sites confirmed their higher trophic status. By linking qPCR-based phytoplankton abundance to chlorophyll a, this study demonstrates that qPCR is a promising avenue to investigate the population dynamics of phytoplankton and the trophic status (or eutrophication) of freshwater rivers.

Bioaccumulation of carcinogenic metals in river fish: A quantitative investigation of public health risk

The rivers are severely contaminated by human activities due to its massive outflow and industrial effluents. Three economically significant fish species Sperata seenghala, Wallago attu, and Cyprinus carpio from the river water were selected for the assessment of heavy metals (HMs). The edible part of the collected fish from the river sampling sites were analyzed to determine the metal concentrations and potential health risks to humans. The Atomic Absorption Spectroscopy (AAS) technique was used to measure HMs including lead (Pb), chromium (Cr), and copper (Cu) for both water and fish samples. Order of HMs in the fish was found as Pb > Cd > Cr. Levels of HMs in all species were found higher than the European Commission (EC) recommended acceptable levels. The metal pollution index (MPI) indicates that W. attu and S. seenghala, two highly consumed fish species, have accumulated HMs in higher concentrations. Wallago attu has the highest bioaccumulation factor (BAF) for both Pb and Cd permissible levels. For adult and children, the Hazard Index (HI) and Targeted Hazard Quotient (THQ) values were less than 1, which indicated that there would be no non-carcinogenic health consequences. Carcinogenic risk (CR) exposure to Pb, Cd, and Cr for all consumers was found to be higher than the permitted limit (10−6-10−4), while children were found more vulnerable than adults in terms of CR values. The study findings identified several sources of contaminants in the area, including agricultural waste, lubricant usage, and electroplating material production dumping near into the river water. Based on the results of the analysis, metal concentrations exceeded the thresholds for carcinogenic health risks, indicating that fish from the area are generally unsafe to consume. Therefore, immediate action must be taken to reduce or prevent the discharge of contaminants into the river. Additionally, regular monitoring of the river's water quality should be conducted to control the contamination of water.

Water quality assessment of Elgo river in Ethiopia using CCME, WQI and IWQI for domestic and agricultural usage

The increasing demand for water due to the escalation in population and aggressive agricultural activities for drinking and irrigation purposes in the rural areas of Ethiopia has put tremendous stress on water requirements. The Elgo River in southern Ethiopia is deteriorating due to sedimentation, soil erosion, stormwater runoff, and anthropogenic activities. Elgo village faces water shortages and a lack of safe drinking water. The purpose of this research was to identify the extent of pollution in Elgo River water using the Canadian Council of Ministers of the Environment (CCME), Water Quality Index (WQI), and Irrigation Water Quality Index (IWQI). A total of 12 water samples were collected from 3 river sampling sites for the dry and wet seasons to test the physicochemical and biological parameters. Results obtained were: turbidity (46.5–156) NTU, colour (103.65–606.5) TCU, EC (182–268) μS/cm, TDS (192.5–275.5) mg/l, TSS (680–2774) mg/l, Ca2+ (22–45) mg/l, Mg2+ (19.5–23.5) mg/l, Cl- (10.5–16.65) mg/l, and SO42- (17.18–47) mg/l for both the dry and wet seasons, respectively. The CCME WQI revealed that the overall results were 38.38 for the dry season and 36.6 for the wet season for drinking water parameters. The CCME WQI categorization indicates that the Elgo River water is classified as poor, with results ranging from 0 to 44. For irrigation purposes 10, parameters such as SAR, PS, PI, MAR, KI, RSC, EC, SSP, TH, and %Na were examined to compute indices using the IWQI model. The overall result of water quality indicated that IWQIs of 81.4 and 62.14 are good for the dry season and poor for the wet season, respectively. This research provides a thorough analysis through modelling to determine the suitability of water for different purposes for the tribal and backward communities of the area.

Using cyanobacteria and other phytoplankton to assess trophic conditions: A qPCR-based, multi-year study in twelve large rivers across the United States

Phytoplankton is the essential primary producer in fresh surface water ecosystems. However, excessive phytoplankton growth due to eutrophication significantly threatens ecologic, economic, and public health. Therefore, phytoplankton identification and quantification are essential to understanding the productivity and health of freshwater ecosystems as well as the impacts of phytoplankton overgrowth (such as Cyanobacterial blooms) on public health. Microscopy is the gold standard for phytoplankton assessment but is time-consuming, has low throughput, and requires rich experience in phytoplankton morphology. Quantitative polymerase chain reaction (qPCR) is accurate and straightforward with high throughput. In addition, qPCR does not require expertise in phytoplankton morphology. Therefore, qPCR can be a useful alternative for molecular identification and enumeration of phytoplankton. Nonetheless, a comprehensive study is missing which evaluates and compares the feasibility of using qPCR and microscopy to assess phytoplankton in fresh water. This study 1) compared the performance of qPCR and microscopy in identifying and quantifying phytoplankton and 2) evaluated qPCR as a molecular tool to assess phytoplankton and indicate eutrophication. We assessed phytoplankton using both qPCR and microscopy in twelve large freshwater rivers across the United States from early summer to late fall in 2017, 2018, and 2019. qPCR- and microscope-based phytoplankton abundance had a significant positive linear correlation (adjusted R2 = 0.836, p-value < 0.001). Phytoplankton abundance had limited temporal variation within each sampling season and over the three years studied. The sampling sites in the midcontinent rivers had higher phytoplankton abundance than those in the eastern and western rivers. For instance, the concentration (geometric mean) of Bacillariophyta, Cyanobacteria, Chlorophyta, and Dinoflagellates at the sampling sites in the midcontinent rivers was approximately three times that at the sampling sites in the western rivers and approximately 18 times that at the sampling sites in the eastern rivers. Welch's analysis of variance indicates that phytoplankton abundance at the sampling sites in the midcontinent rivers was significantly higher than that at the sampling sites in the eastern rivers (p-value = 0.013) but was comparable to that at the sampling sites in the western rivers (p-value = 0.095). The higher phytoplankton abundance at the sampling sites in the midcontinent rivers was presumably because these rivers were more eutrophic. Indeed, low phytoplankton abundance occurred in oligotrophic or low trophic sites, whereas eutrophic sites had greater phytoplankton abundance. This study demonstrates that qPCR-based phytoplankton abundance can be a useful numerical indicator of the trophic conditions and water quality in freshwater rivers.

Abundance trends for river macroinvertebrates vary across taxa, trophic group and river typology.

There is mounting evidence that terrestrial arthropods are declining rapidly in many areas of the world. It is unclear whether freshwater invertebrates, which are key providers of ecosystem services, are also declining. We addressed this question by analysing a long-term dataset of macroinvertebrate abundance collected from 2002 to 2019 across 5009 sampling sites in English rivers. Patterns varied markedly across taxonomic groups. Within trophic groups we detected increases in the abundance of carnivores by 19% and herbivores by 14.8%, while we estimated decomposers have declined by 21.7% in abundance since 2002. We also found heterogeneity in trends across rivers belonging to different typologies based on geological dominance and catchment altitude, with organic lowland rivers having generally higher rates of increase in abundance across taxa and trophic groups, with siliceous lowland rivers having the most declines. Our results reveal a complex picture of change in freshwater macroinvertebrate abundance between taxonomic groups, trophic levels and river typologies. Our analysis helps with identifying priority regions for action on potential environmental stressors where we discover macroinvertebrate abundance declines.

Water pollution at Lake Natron Ramsar site in Tanzania: A threat to aquatic life

Lake Natron Ramsar site is a recognized Ramsar site and Important Bird and Biodiversity Area (IBA) in Tanzania. It is a breeding site for 75% of the global population of lesser flamingo (Phoeniconaias minor). Water quality changes can affect the plankton composition and diversity, causing alteration of LNRS ecosystem abundance. Water quality assessment at LNRS was conducted after reported alkaline tilapines (Alcolapia species) and pelican (Pelecanus onocrotalus) mortalities. Water samples were collected from five sampling sites in Engaresero and Pinyinyi rivers during wet and dry seasons. Physico-chemical parameters including pH, turbidity, dissolved oxygen (DO), biological oxygen demand (BOD), chemical oxygen demand (COD), nitrates, chloride, sodium, ammonium, electrical conductivity and total alkalinity were assessed. Additionally, plankton composition and abundance were analyzed to investigate the cause of reported mortalities. Observation method was used to assess the anthropogenic activities taking place along inlet rivers. BOD, COD, ammonium and pH values were higher compared to the Tanzania Environmental Management Act of 2004 and World Health Organization recommended values for freshwater, resulting into low DO levels. There were no zooplankton or phytoplankton colonies observed from sampled water. The study observed anthropogenic activities (unsustainable crop cultivation, deforestation and overgrazing) at the LNRS catchment. The reported physico-chemical changes could be due to such anthropogenic activities at the LNRS catchment which could have resulted into the reported fish mortalities. Watershed management using nature-based solutions such as restoration of riparian vegetation and environment management awareness are recommended for LNRS ecosystem sustainability.

Human land use impacts viral diversity and abundance in a New Zealand river.

Although water-borne viruses have important implications for the health of humans and other animals, little is known about the impact of human land use on viral diversity and evolution in water systems such as rivers. We used metatranscriptomic sequencing to compare the diversity and abundance of viruses at sampling sites along a single river in New Zealand that differed in human land-use impacts, ranging from pristine to urban. From this, we identified 504 putative virus species, of which 97 per cent were novel. Many of the novel viruses were highly divergent and likely included a new subfamily within the Parvoviridae. We identified at least sixty-three virus species that may infect vertebrates—most likely fish and water birds—from the Astroviridae, Birnaviridae, Parvoviridae, and Picornaviridae. No putative human viruses were detected. Importantly, we observed differences in the composition of viral communities at sites impacted by human land use (farming and urban) compared to native forest sites (pristine). At the viral species level, the urban sites had higher diversity (327 virus species) than the farming (n = 150) and pristine sites (n = 119), and more viruses were shared between the urban and farming sites (n = 76) than between the pristine and farming or urban sites (n = 24). The two farming sites had a lower viral abundance across all host types, while the pristine sites had a higher abundance of viruses associated with animals, plants, and fungi. We also identified viruses linked to agriculture and human impact at the river sampling sites in farming and urban areas that were not present at the native forest sites. Although based on a small sample size, our study suggests that human land use can impact viral communities in rivers, such that further work is needed to reduce the impact of intensive farming and urbanisation on water systems.

Mixture of Toxic Metals and Volatile Organic Compounds in a River Induces Cytotoxicity

Pollution of aquatic ecosystems due to toxic mixtures is a worldwide problem associated with the increase of wastewater discharges that causes problems to human health and biodiversity. This study aims to evaluate the cytotoxic potential of water from the Atoyac River. Meristems of Allium cepa L. were exposed to water samples from the Atoyac River with different concentrations for 120 hours. Pearson correlation was used to investigate the relationship between contaminants and cytotoxicity. The results corroborated the cytotoxic effect of the mixture of agents such as toxic metals and volatile organic compounds found in all river sampling sites. The Allium cepa test showed decreased mitotic alterations in prophase and metaphase indices. There was a strong negative association between the concentration of toxic metals and volatile organic compounds and the cytotoxic effect. The observations of cytotoxic effects show that the contaminant mixture contains aneugenic agents which prevent the synthesis and fixation of fibers of the mitotic spindle to the kinetochore, which prevents the displacement of the chromosomes. This study shows the need to study the effects at the cellular and molecular level in heavily polluted rivers to prevent negative effects on exposed ecosystems and populations.

Development and testing of an environmental DNA (eDNA) assay for endangered Atlantic sturgeon to assess its potential as a monitoring and management tool

AbstractSignificant declines in Atlantic sturgeon (Acipenser oxyrhynchus oxyrhynchus) abundances along the US east coast have spurred major research efforts and management actions over the last 50 years, yet information on spawning stock abundances and habitat use is still lacking for many river systems, including in the Chesapeake Bay, USA. Here, we developed and tested a new quantitative PCR (qPCR) assay to detect Atlantic sturgeon environmental DNA (eDNA) in water samples with the goal of providing an alternative method to monitor presence and relative abundance. We also examined Atlantic sturgeon eDNA shedding rates in laboratory experiments. A qPCR‐probe assay targeting Cytochrome B was developed and showed no amplification of other related and co‐occurring fishes. Pond trials at a density of ~0.2 g/L sturgeon produced relatively strong eDNA detections (~1,000–25,000 copies/L) in all seven water samples assayed. Water samples taken from two river systems in the Chesapeake Bay produced zero eDNA detections in the summer, while fall sampling during sturgeon spawning produced positive eDNA detections in 26% of samples, though at much lower concentrations (400–1,800 copies/L) compared with the pond (mesocosm) detections. Acoustic detections of sturgeon near river sampling sites were positively associated with eDNA detections. However, the eDNA assay failed to detect the presence of sturgeon in some samples when abundances were very low or when fish were in deep water. Finally, Atlantic sturgeon eDNA shedding rates were estimated to be on the order of estimates for other fish species, which suggests that relatively weak detections in the field are not necessarily driven by low rates of eDNA shedding. Overall, eDNA analysis represents a promising new monitoring tool for Atlantic sturgeon. Applying these methods in other rivers along the US east coast is an important next step in documenting Atlantic sturgeon distribution for management and conservation purposes.

Application of GIS for water quality monitoring in the aquaculture impacted Rawang sub-basin of the Selangor river, Malaysia

Freshwater aquaculture is a prominent activity in Rawang sub-basin of the Selangor River. Despite the importance good water quality in daily life, there is limited study on the impact of aquaculture activities on water quality. This paper discusses water quality parameter status (pH, dissolved oxygen (DO), ammoniacal nitrogen, turbidity, total suspended solids (TSS), chemical oxygen demand (COD) and biochemical oxygen demand (BOD)) based on Water Quality Index (WQI) in the aquaculture-impacted Rawang sub-basin of Selangor River and develops the Inverse Distance Weighted (IDW) maps showing water quality status by using GIS (ArcGIS 10.2.1 software) in order to identify the potential aquaculture impacted sites. Seven river sampling sites were selected including Guntong (SR1), Guntong’s tributaries (SR2/control), Kuang (SR3 and SR7), Gong (SR4), Buaya (SR5), and Serendah (SR6) using random sampling techniques based on accessibility and proximity to aquaculture farms. Seven water quality parameters were recorded and analysed on a bi-monthly basis. Results revealed that Guntong, Kuang, Buaya and Serendah rivers had moderate water quality, whereas Gong River was significantly polluted. The control river recorded clean water quality status. One-way analysis of variance (ANOVA) showed that there were significant differences in all measured water quality parameters among sampling sites (P<0.05).

崇明岛内河夏季鱼类群落组成及分布特征

PDF HTML阅读 XML下载 导出引用 引用提醒 崇明岛内河夏季鱼类群落组成及分布特征 DOI: 10.5846/stxb202002240321 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 上海市科委项目（19DZ1203801）；国家自然科学基金（41977344，41761144062） Community composition and distribution characteristics of the fish assemblages in the rivers of Chongming Island in summer Author: Affiliation: Fund Project: Science and Technology Committee of Shanghai Municipality, The National Natural Science Foundation of China 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:崇明岛为世界最大的河口冲积沙岛，岛内河网水系发达。2018年6月，选择崇明岛内主要代表性河道包括北横引河、南横引河和竖河（庙港、堡镇港、八滧港），设置16个固定站点，对内河鱼类开展了拖网调查，分析了崇明岛内河夏季鱼类群落组成及分布特征。调查期间共采集到鱼类4417尾，分属6目9科30种，主要为鲤形目鲤科鱼类；其中，淡水鱼类27种，洄游鱼类2种，近海鱼类1种。从空间分布来看，北横引河记录鱼类20种，南横引河记录鱼类17种，竖河记录鱼类18种，不同河道鱼类种类组成及优势种特征存在明显差异，其共有优势种为鲫Carassius auratus、似鳊Pseudobrama simoni和翘嘴红鲌Erythroculter ilishaeformis，部分种类只在特定的河段有记录。北横引河各站点平均物种数、丰度和生物量均高于南横引河和竖河，但3类河道间不存在显著差异（P>0.05）；主要优势种鲫、翘嘴红鲌和似鳊平均体长均以北横引河为最大，且在南横引河与北横引河中的个体体长均存在极显著差异（P<0.01）。聚类和排序分析结果表明，北横引河和南横引河鱼类组成差异较大，而竖河介于两者之间。相似性分析的结果也表明，南、北横引河鱼类群落结构差异极显著（R=0.524，P=0.008），且这两组间鱼类种类组成的平均相异性最高，为43.92%，造成两组间差异的主要物种有10种（贡献率均大于5%）。综合来看，崇明岛内河鱼类群落组成与分布特征不同于一般的内陆河流，而具有河口型岛屿的特点。未来需要在长期监测基础上，综合多种因素，对崇明岛内河鱼类组成、分布、变化趋势及内在机理开展系统研究。 Abstract:Chongming Island is the largest estuarine alluvial island in the world. It has a well-developed river network. In June 2018, trawl surveys on the fish were carried out in 16 fixed sampling sites in the representative rivers of Chongming Island, including the Nanheng Canal, Beiheng Canal and also the vertical canals Miaogang, Baozhengang, and Bayaogang. The composition and distribution characteristics of the fish communities in summer were analyzed based on these surveys. A total of 4417 specimens belonging to 6 orders, 9 families and 30 species of the fishes were collected in the surveys. Among them, 27 species were freshwater fish, 2 species were migratory fish and 1 species was coastal marine fish. And 20, 17 and 18 fish species were recorded in the Beiheng Canal, Nanheng Canal, and the vertical canals respectively. The characteristics of the fish assemblages, including the dominant species, varied among the rivers. The common dominant species of all the rivers were Carassius auratus, Pseudogramma simoni and Erythroculter ilishaeformis. Some species were only recorded in some specific reaches. The average species number, abundance and biomass of the fish in the Beiheng Canal were much higher than those in the Nanheng Canal and also the vertical canals, but there was no significant difference among the rivers (P>0.05). The average body length of major dominant species, Carassius auratus, Pseudogramma simoni and Erythroculter ilishaeformis in the Beiheng Canal were also the largest, and there was a very significant difference in individual body length between the Nanheng Canal and the Beiheng Canal (P<0.01). Cluster and nMDS analysis showed the greater difference between the fish assemblages in the Beiheng Canal and the Nanheng Canal, while those in the vertical canals were between these two. The results of ANOSIM also showed that the difference of the fish community structure between the Beiheng Canal and the Nanheng Canal was extremely significant (R=0.524, P=0.008), the average dissimilarity between these two groups appeared to be the highest at 43.92%, and there were 10 main species causing such difference (contribution rate was greater than 5%).In general, we found out that the community composition and distribution characteristics of the fish assemblages in the rivers of Chongming Island were different from those of general inland rivers, while had some characteristics of the estuarine islands. In the future, comprehensive studies on the community composition, distribution characteristics, developing trends and the corresponding mechanisms of the fishes in the rivers of Chongming Island should be carried out based on the long term monitoring. 参考文献 相似文献 引证文献

Spatiotemporal Differentiation and Degradation Analysis of Polybrominated Diphenyl Ethers in Sediments of Shanmei Reservoir and Its Inflowing River, Quanzhou, China

To investigate the spatiotemporal differentiation of polybrominated diphenyl ethers (PBDEs) in urban water-source reservoirs and degradation sources of BDE homologues and their contributions, we analyzed the contents, pollution degrees, spatial distributions, hydrological period changes, inventories, profiles, and degradation source contributions of PBDEs in the surface sediments of Shanmei Reservoir and its inflowing river, Quanzhou, China. The results showed that the median ∑PBDEs (1072.1 ng ·g-1) in the inflowing river sediment was 6.7 times than that of the reservoir (160.4 ng ·g-1) and the total amount of ∑PBDEs in sediments per unit area (80.3 kg ·km-2) was 6.3 times than that of Taihu Lake and 188 times than that of the Great Lakes in North America. The pollution degrees of PBDEs in Shanmei Reservoir were more severe than those of most lakes and reservoirs at home and abroad, which was dominated by BDE-209 (84.5%-99.2%). Most of the sampling sites in the reservoir (r 0.564-0.994, P<0.034) and the inflowing river (r 0.953-1.0, P<0.000) had high similarity in the composition of PBDEs. Significantly positive correlations (r 0.779-0.964, P<0.005) were observed between the reservoir entry area and river sampling sites, which were stronger than the other functional areas, indicating that the inflowing river was a major pollution source of PBDEs in the Shanmei Reservoir. The tail region of the reservoir had low correlations with the inflowing river (r 0.454-0.915, P≤0.128), and was relatively much more affected by Jiudu Town. The changes in hydrological period of the ∑PBDEs were relatively consistent at each sampling site (r 0.617-0.714, P≤0.077), but the impact of the changes in the hydrological period on the ∑PBDEs was not statistically significant (P=0.178, Two-Way ANOVA). However, the site changes had a significant influence on the ∑PBDEs (P=0.0001), and significant or nearly differences were observed between the reservoir entry area and other functional areas (P 0.019-0.061), indicating that the spatial distribution variations of the PBDEs in reservoir sediments were greater than the changes in hydrological period. The natural degradation of the PBDEs gradually increased from the river to the reservoir entry area and then to the central reservoir area. The reductive debromination rates varied at different brominated levels, and some BDE homologues accumulated due to their slowly continued degradation velocities. Research on abundance ratios indicated that the lower brominated BDE homologues were mainly derived from the natural degradation of decabromodiphenyl ether by stepwise reductive debromination. Approximately 70% of Nona-BDE produced by Deca-BDE degradation could rapidly be degraded to form Octa-BDE. Approximately 85% of BDE-208 was derived from the degradation of BDE-209. During the degradation process from Octa-BDE to Penta-BDE, some Octa-BDE and Hexa-BDE homologues accumulated due to relatively slower degradation velocities, and the degradation rates of Penta-BDE to Tri-BDE were above 70%.

Chemometrics characterization of The Llobregat river dissolved organic matter

The potential use of multivariate chemometric methods for the investigation of the dissolved organic matter (DOM) sources in surface river waters monitored by UV absorbance and excitation-emission fluorescence spectroscopy is shown. The Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) method is applied to the analysis of the excitation-emission multidimensional data matrices acquired at ten different river sampling sites along the basin of the Llobregat river (located at the north-west of the Iberian Peninsula), from its origin to its mouth, during three different monitoring campaigns. The results obtained by MCR-ALS provided information about the compositional changes of the different DOM sources (i.e. humic-, fulvic- or protein-like sources) present in the investigated river samples, about their site distribution along the river basin and about their changes in three different monitoring campaigns. Eight different MCR-ALS components were resolved describing several types of DOM, with different excitation and emission spectra and with distinct distribution profiles along the river sampling sites. A comparative study of each single sampling site describing the changes in their DOM composition during the three monitoring campaigns was also performed.

Survey and assessment of metals distribution in the overlying water of the representative lake and rivers in Zhenjiang, China

In the current study, overlying water samples were collected from 21 sampling sites in representative lake and rivers in Zhenjiang, China, during winter and summer. The concentrations of As, Cd, Cr, Cu, Pb and Zn were determined, and the pollution index was assessed using single-factor and Nemerow pollution indices. Results showed that (1) the average As, Cd, Cr, Cu, Pb and Zn concentrations were 3.75, 0.50, 2.25, 3.33, 1.30 and 21.92 μg/L in the winter samples and 3.62, 0.40, 1.91, 2.72, 1.23 and 30.76 μg/L in the summer samples, respectively. The concentrations of these elements were within the concentration ranges of published data on rivers or lakes in China. (2) ANOVA revealed obvious spatial, not seasonal, variations in the overlying water in this research area, except for Cd and Zn. (3) The single-factor and Nemerow pollution index values were below 0.7 in all sites in the winter and summer samples, indicating that the water body had not been affected by As, Cd, Cr, Cu, Pb and Zn yet; the water quality was relatively good and free from the influence of these pollutants. (4) A significant correlation was found amongst As, Cd, Cr, Cu and Pb (P 0.05), except for Zn and Cu (P < 0.05) in the winter samples. Principal component analysis (PCA) verified that As, Cd, Cr, Cu and Pb had similar distribution patterns. The correlations and PCA results indicated that the possible source of As, Cd, Cr, Cu and Pb was geochemical role, whereas complex original sources caused by human activities may have affected Zn concentrations in this research region.

Spatio-temporal variability of water pollution by chlordecone at the watershed scale: what insights for the management of polluted territories?

Chlordecone, applied on soils until 1993 to control banana weevil, has polluted water resources in the French West Indies for more than 40years. At the watershed scale, chlordecone applications were not homogenous, generating a spatial heterogeneity of the pollution. The roles of climate, hydrology, soil, agronomy, and geology on watershed functioning generate a temporal heterogeneity of the pollution. This study questions the interactions between practices and the environment that induce such variability. We analyzed hydrological and water pollution datasets from a 2-year monitoring program on the Galion watershed in Martinique (French West Indies). We conjointly analyzed (i) weekly chlordecone (CLD) concentration monitored on 3 river sampling sites, (ii) aquifer piezometric dynamics and pollutions, and (iii) agricultural practices on polluted soils. Our results showed that chlordecone pollution in surface waters are characterized by annual trends and infra-annual variations. Aquifers showed CLD concentration 10 times higher than surface water, with CLD concentration peaks during recharge events. We showed strong interactions between rainfall events and practices on CLD pollution requiring a systemic management approach, in particular during post-cyclonic periods. Small sub-watershed with high CLD pollution appeared to be a substantial contributor to CLD mass transfers to the marine environment via rivers and should therefore receive priority management. We suggest increasing stable organic matter return to soil as well as external input of organic matter to reduce CLD transfers to water. We identified hydrological conditions-notably drying periods-and tillage as the most influential factors on CLD leaching. In particular, tillage acts on 3 processes that increases CLD leaching: organic matter degradation, modification of water paths in soil, and allophane clay degradation.

Prevalence, levels and seasonal variations of human enteric viruses in six major rivers in Alberta, Canada

The prevalence and seasonal variation of 7 viruses in 6 major rivers in Alberta were assessed using a combination of qPCR, cell culture and integrated cell culture with qPCR (ICC-qPCR). Water samples were collected monthly from rivers at different sites upstream and downstream of major urban centers. Seven viruses including rotavirus, adenovirus, astrovirus, norovirus, sapovirus, JC virus and enterovirus, were detected in at least one of the water samples at each site using qPCR. Rotavirus was most common with concentration ranging from 2.3 to 4.5 log10 genomic equivalent (GE) copies/L. Norovirus, sapovirus, astrovirus, adenoviruses and JC virus peaked during the winter (November to March). Viruses were most prevalent at the Bow River sampling site downstream of the City of Calgary, followed by the North Saskatchewan River site downstream of the City of Edmonton and the Red Deer River site downstream of the City of Red Deer. The detection rates and quantity of viruses had significant difference in the sampling sites between upstream and downstream of major urban centers (p < 0.001). 14% of the samples tested positive using viral culture indicating the presence of infectious viruses in river. Sequencing analysis identified human rotavirus in 75% of the samples collected from downstream versus 37% of the samples collected from upstream sites (p < 0.02). Multivariate binary regression showed that human activity in watersheds is a significant determinant of viruses in Alberta's Rivers. The discharge from wastewater treatment plants may be the possible sources of viral contamination. Seasonal coincidence of acute viral gastroenteritis outbreaks and monthly peak occurrence of enteric viruses in river water implies potential impact of waterborne viruses on human health.

Arsenic Geochemistry of Alluvial Sediments and Pore Waters Affected by Mine Tailings along the Belle Fourche and Cheyenne River Floodplains

Gold mining operations in the northern Black Hills of South Dakota resulted in the discharge of arsenopyrite-bearing mine tailings into Whitewood Creek from 1876 to 1977. Those tailings were transported further downstream along the Belle Fourche River, the Cheyenne River, and the Missouri River. An estimated 110 million metric tons of tailings remain stored in alluvial deposits of the Belle Fourche and Cheyenne Rivers. Pore-water dialysis samplers were deployed in the channel and backwaters of the Belle Fourche and Cheyenne Rivers to determine temporal and seasonal changes in the geochemistry of groundwater in alluvial sediments. Alluvial sediment adjacent to the dialysis samplers were cored for geochemical analysis. In comparison to US Environmental Protection Agency drinking water standards and reference concentrations of alluvial sediment not containing mine tailings, the Belle Fourche River sites had elevated concentrations of arsenic in pore water (2570 μg/L compared to 10 μg/L) and sediment (1010 ppm compared to < 34 ppm), respectively. Pore water arsenic concentration was affected by dissolution of iron oxyhydroxides under reducing conditions. Sequential extraction of iron and arsenic from sediment cores indicates that substantial quantities of soluble metals were present. Dissolution of arsenic sorbed to alluvial sediment particles appears to be affected by changing groundwater levels that cause shifts in redox conditions. Bioreductive processes did not appear to be a substantial transport pathway but could affect speciation of arsenic, especially at the Cheyenne River sampling sites where microbial activity was determined to be greater than at Belle Fourche sampling sites.

Evaluating organochlorine pesticide residues in the aquatic environment of the Lake Naivasha River basin using passive sampling techniques

Passive sampling techniques can improve the discovery of low concentrations by continuous collecting the contaminants, which usually go undetected with classic and once-off time-point grab sampling. The aim of this study was to evaluate organochlorine pesticide (OCP) residues in the aquatic environment of the Lake Naivasha river basin (Kenya) using passive sampling techniques. Silicone rubber sheet and Speedisk samplers were used to detect residues of α-HCH, β-HCH, γ-HCH, δ-HCH, heptachlor, aldrin, heptachlor epoxide, pp-DDE, endrin, dieldrin, α-endosulfan, β-endosulfan, pp-DDD, endrin aldehyde, pp-DDT, endosulfan sulfate, and methoxychlor in the Malewa River and Lake Naivasha. After solvent extraction from the sampling media, the residues were analyzed using gas chromatography electron capture detection (GC-ECD) for the OCPs and gas chromatography-mass spectrometry (GC-MS) for the PCB reference compounds. Measuring the OCP residues using the silicone rubber samplers revealed the highest concentration of residues (∑OCPs of 81 (± 18.9 SD) μg/L) to be at the Lake site, being the ultimate accumulation environment for surficial hydrological, chemical, and sediment transport through the river basin. The total OCP residue sums changed to 71.5 (± 11.3 SD) μg/L for the Middle Malewa and 59 (± 12.5 SD) μg/L for the Upper Malewa River sampling sites. The concentration sums of OCPs detected using the Speedisk samplers at the Upper Malewa, Middle Malewa, and the Lake Naivasha sites were 28.2 (± 4.2 SD), 31.3 (± 1.8 SD), and 34.2 (± 6.4 SD) μg/L, respectively. An evaluation of the different pesticide compound variations identified at the three sites revealed that endosulfan sulfate, α-HCH, methoxychlor, and endrin aldehyde residues were still found at all sampling sites. However, the statistical analysis of one-way ANOVA for testing the differences of ∑OCPs between the sampling sites for both the silicone rubber sheet and Speedisk samplers showed that there was no significant difference from the Upper Malewa to the Lake site (P < 0.05). Finally, the finding of this study indicated that continued monitoring of pesticides residues in the catchment remains highly recommended.