This study aims to determine the structure and composition of the natural forest vegetation of Negeri Amahusu, Nusaniwe District, Ambon City. The sampling method used in this study is a combination of the grid line method and the path method to study the composition and structure of vegetation at the research location. In this study, 5 (five) observation paths were made, each path consisting of 5 plots with a size of 20 x 20 m for the tree level so that the observation path measures 20 x 100 m. While the sample plots for the pole level were made 10 x 10 m, the sapling level 5 x 5 m, and the seedling level 2 x 2 m. The results of the study showed that the Amahusu natural forest consists of 4 Stratifications (Strata A, B, D, and E) dominated by stratum B, where the height of stratum A trees ranges from 20-25 m while stratum B ranges from 8-19 m with dominant species including: Durian (Durio zibethinus), Canary (Canarium commune), and Linggua (Pterocarpus indicus). The composition of Amahusu forest species at the seedling level consists of 18 species, 20 species of saplings, 15 species of poles and 15 species of trees. The species diversity index is included in the medium category with an H' value at the seedling level of 1.148, saplings 1.174, poles 1.018 and trees 1.072.
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